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Growth of Candida albicans in dexamethasone-supplemented media. 白色念珠菌在添加地塞米松培养基中的生长。
Sabouraudia Pub Date : 1985-07-01 DOI: 10.1080/00362178585380441
M. Ghannoum, G. Burns, K. Abu Elteen
{"title":"Growth of Candida albicans in dexamethasone-supplemented media.","authors":"M. Ghannoum, G. Burns, K. Abu Elteen","doi":"10.1080/00362178585380441","DOIUrl":"https://doi.org/10.1080/00362178585380441","url":null,"abstract":"Candida albicans grown in dexamethasone (DXM) shows an apparent increase in dry weight. This increase, however, represents an artefact due to entrapment and incorporation of DXM by the yeast. Thus opportunistic infections by C. albicans which are promoted by DXM must be due entirely to effects other than growth enhancement of the organism.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"115 1","pages":"313-5"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82029315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Corneal transplant infection by Paecilomyces lilacinus. 淡紫拟青霉角膜移植感染。
Sabouraudia Pub Date : 1985-07-01 DOI: 10.1080/00362178585380421
Morris A. Gordon, Sylvia W. Norton
{"title":"Corneal transplant infection by Paecilomyces lilacinus.","authors":"Morris A. Gordon, Sylvia W. Norton","doi":"10.1080/00362178585380421","DOIUrl":"https://doi.org/10.1080/00362178585380421","url":null,"abstract":"Paecilomyces lilacinus was cultured from an ulcerated corneal transplant, sections of which, following surgical removal, revealed dense mycelial growth throughout the corneal remnant and penetrating Descemet's membrane. PAS-stained sections showed, in addition to the hyphae, numerous small, ovoid elements consistent with conidia of P. lilacinus. Infection apparently had spread to the transplant from the recipient's cornea. The fungal isolate proved resistant, in vitro, to amphotericin B, 5-fluorocytosine, and pimaricin, but sensitive to ketoconazole and moderately sensitive to miconazole. Following a second transplant and intensive antifungal therapy, the infection appears to have been eliminated.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"432 1","pages":"295-301"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82873164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 53
Oral treatment with ketoconazole in systemic candidosis of guinea-pigs: microbiology, hematology and histopathology. 酮康唑口服治疗豚鼠全身念珠菌病:微生物学、血液学和组织病理学。
Sabouraudia Pub Date : 1985-06-01 DOI: 10.1080/00362178585380291
J Van Cutsem, J Fransen, F Van Gerven, P A Janssen
{"title":"Oral treatment with ketoconazole in systemic candidosis of guinea-pigs: microbiology, hematology and histopathology.","authors":"J Van Cutsem,&nbsp;J Fransen,&nbsp;F Van Gerven,&nbsp;P A Janssen","doi":"10.1080/00362178585380291","DOIUrl":"https://doi.org/10.1080/00362178585380291","url":null,"abstract":"<p><p>Non-pretreated Albino guinea-pigs were infected intravenously with Candida albicans and treated orally either with placebo or ketoconazole. The 17-day follow-up of the fungal dissemination was based upon hematology, on gross and microscopic lesions and on the demonstration of the fungus by culture techniques. The efficacy of ketoconazole, both with regard to the quantity and the morphology of fungi in various organs and the tissue-healing process are discussed. The treatment of human systemic candidosis will also be considered. No side-effects due to the therapy were observed in these experiments.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"189-98"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15136827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Purification and partial characterization of an exocellular proteinase from Trichophyton rubrum. 红毛癣菌胞外蛋白酶的纯化及部分特性研究。
Sabouraudia Pub Date : 1985-06-01 DOI: 10.1080/00362178585380271
A K Sanyal, S K Das, A B Banerjee
{"title":"Purification and partial characterization of an exocellular proteinase from Trichophyton rubrum.","authors":"A K Sanyal,&nbsp;S K Das,&nbsp;A B Banerjee","doi":"10.1080/00362178585380271","DOIUrl":"https://doi.org/10.1080/00362178585380271","url":null,"abstract":"<p><p>An exocellular proteinase produced by Trichophyton rubrum in a glucose-peptone broth was purified from lyophilized and dialysed culture filtrate of the dermatophyte by Sephadex G-100 gel filtration and preparative polyacrylamide gel electrophoresis. The purified enzyme was a homogeneous protein of molecular weight 34700 and it could hydrolyse azoalbumin, casein, bovine serum albumin, alpha-N-benzoyl-L-arginine ethyl ester and p-toluenesulfonyl-L-arginine methyl ester but not N-benzoyl-L-tyrosine ethyl ester, alpha-N-benzoyl-DL-arginine-p-nitroanilide and keratin. The enzyme showed an alkaline pH optimum and was not activated by divalent metal ions but inhibited strongly by phenylmethylsulfonylfluoride. Thus the enzyme was identified as an alkaline serine proteinase.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"165-78"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15012181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 70
Effect of mycolase and amphotericin B on Candida albicans and Candida pseudotropicalis in vitro and in vivo. 霉菌酶和两性霉素B对白色念珠菌和假热带念珠菌体外和体内的影响。
Sabouraudia Pub Date : 1985-06-01
L J Chalkley, A P Trinci, A M Pope
{"title":"Effect of mycolase and amphotericin B on Candida albicans and Candida pseudotropicalis in vitro and in vivo.","authors":"L J Chalkley,&nbsp;A P Trinci,&nbsp;A M Pope","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A mixture of enzymes (mycolase) capable of lysing yeast cell walls was prepared from culture filtrates of Physarum polycephalum. The enzymes present in mycolase included chitinase, beta-1,3-glucanases and exo-glycosidases. The pH optima of these enzymes were in the range 3.5-5.0 and they had low activities at pH 7.0. Mycolase produced spheroplasts from Candida pseudotropicalis and, unlike commercial enzyme preparations such as L1, chitinase, beta, 1,3-glucanase and beta-glucosidase, had some candicidal activity in vitro against C. pseudotropicalis and C. albicans. Mycolase potentiated the antifungal activity of amphotericin B against C. pseudotropicalis grown in shake flask culture but did not potentiate the antifungal activity of the antibiotic against similar cultures of C. albicans; indeed antagonism between mycolase and amphotericin B was sometimes observed with the latter yeast. Mycolase caused an approximately two-fold increase in the total and viable counts of cultures of C. albicans inoculated with stationary phase cells. These increases, which were observed within about 30 min, were attributed to mycolase inducing the premature release of viable buds from 'lag' phase cells. Mycolase also increased the rate at which C. albicans formed germ tubes when the yeast was cultured in a medium containing serum. Mycolase alone or in combination with amphotericin B did not appreciably enhance phagocytosis or intracellular killing of the yeasts by unstimulated mouse peritoneal macrophages. Studies on mice infected systemically with C. albicans showed that mycolase only slightly enhanced amphotericin B therapy.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"147-64"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15012180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular and cellular events during the yeast to mycelium transition in Sporothrix schenckii. 申氏孢子丝菌酵母向菌丝转化过程中的分子和细胞事件。
Sabouraudia Pub Date : 1985-06-01 DOI: 10.1080/00362178585380311
S Betancourt, L J Torres-Bauzá, N Rodríguez-Del Valle
{"title":"Molecular and cellular events during the yeast to mycelium transition in Sporothrix schenckii.","authors":"S Betancourt,&nbsp;L J Torres-Bauzá,&nbsp;N Rodríguez-Del Valle","doi":"10.1080/00362178585380311","DOIUrl":"https://doi.org/10.1080/00362178585380311","url":null,"abstract":"<p><p>Unbudded singlets from exponentially growing yeast cells of Sporothrix schenckii were harvested, selected by filtration and allowed to form germ tubes in a basal medium with glucose at pH 4.0 and 25 degrees C. These conditions supported only the development of the mycelial form of S. schenckii in a reproducible manner which allowed further analysis of the early cellular events occurring during the yeast-to-mycelium transition. The relationship between macromolecular synthesis (DNA and RNA synthesis) and nuclear division, hyphal growth and septum formation were investigated during germ tube formation. RNA synthesis started 0 to 3 h after the induction of germ tube formation, followed by DNA synthesis and the first nuclear division, which took place between 3 and 6 h. Germ tube formation followed nuclear division and was first evidenced 6 h after the induction of germ tube formation, but was not completed until 12 h after inoculation. Septation was first observed in these germ tubes at the mother cell-germ tube junction 6 h after induction. Addition of hydroxyurea, an inhibitor of DNA synthesis, to the medium, also inhibited nuclear division and germ tube growth, suggesting that these processes in S. schenckii are dependent upon DNA synthesis.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"207-18"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15136829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Contribution of complement component C5 to the pathogenesis of experimental murine cryptococcosis. 补体组分C5在实验性小鼠隐球菌病发病机制中的作用。
Sabouraudia Pub Date : 1985-06-01 DOI: 10.1080/00362178585380331
J C Rhodes
{"title":"Contribution of complement component C5 to the pathogenesis of experimental murine cryptococcosis.","authors":"J C Rhodes","doi":"10.1080/00362178585380331","DOIUrl":"https://doi.org/10.1080/00362178585380331","url":null,"abstract":"<p><p>C5-deficient (C5-) mice succumb much sooner after intravenous inoculation with Cryptococcus neoformans than do C5-sufficient (C5+) mice. The C5- mice developed acute, fatal cryptococcal pneumonia, whereas the C5+ mice did not. The pneumonia was characterized by lung viable counts in C5- mice up to 1000-fold higher than in C5+, initial sequestration of twice as much 59Fe-labeled C. neoformans, and subsequent development of pulmonary edema. Chemotaxis of heterophils (PMNs) and mononuclear cells in response to C. neoformans was markedly greater in C5+ mice than in C5- animals. The effect of C5 on localization and growth of C. neoformans in the lung appeared to account for the disparate survival times of C5+ and C5+ mice after intravenous inoculation with C. neoformans.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"225-34"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15136715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 61
Itraconazole treatment of murine aspergillosis. 伊曲康唑治疗小鼠曲霉病。
Sabouraudia Pub Date : 1985-06-01 DOI: 10.1080/00362178585380321
J R Graybill, J Ahrens
{"title":"Itraconazole treatment of murine aspergillosis.","authors":"J R Graybill,&nbsp;J Ahrens","doi":"10.1080/00362178585380321","DOIUrl":"https://doi.org/10.1080/00362178585380321","url":null,"abstract":"<p><p>ICR mice were challenged with conidia of Aspergillus fumigatus given either intranasally or intravenously, and treated beginning 1 day later with itraconazole, amphotericin B, or the vehicles for these two agents. Mice challenged intravenously and treated with either antifungal drug had prolonged survival over controls, and had lower tissue counts in the kidneys than the controls. However, mice challenged intranasally had neither prolonged survival nor lower lung tissue counts than the controls.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"219-23"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14126677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Experimental chronic vaginal candidosis in rats. 实验性大鼠慢性阴道念珠菌病。
Sabouraudia Pub Date : 1985-06-01 DOI: 10.1080/00362178585380301
J D Sobel, G Muller, J F McCormick
{"title":"Experimental chronic vaginal candidosis in rats.","authors":"J D Sobel,&nbsp;G Muller,&nbsp;J F McCormick","doi":"10.1080/00362178585380301","DOIUrl":"https://doi.org/10.1080/00362178585380301","url":null,"abstract":"<p><p>In the past, the rat model of experimental vaginal candidosis has been used to study the efficacy of antifungal agents in eradicating acute vaginitis. In the present study, chronic vaginal candidosis was induced to study the natural history of the infection. Results indicate that chronic infection is readily achieved and is strictly dependent on oophorectomy and hormonal maintenance of pseudoestrous. Histologic studies confirm that rats so infected and with long term vaginal carriage of Candida albicans have true chronic infection with extensive mycelial formation and superficial mucosal invasion.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"199-206"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380301","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15136828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 65
Phaeoannellomyces and the Phaeococcomycetaceae, new dematiaceous blastomycete taxa. phaoannellomyces和phaococcomycetaceae，新母芽菌分类群。
Sabouraudia Pub Date : 1985-06-01
M R McGinnis, W A Schell, J Carson
{"title":"Phaeoannellomyces and the Phaeococcomycetaceae, new dematiaceous blastomycete taxa.","authors":"M R McGinnis,&nbsp;W A Schell,&nbsp;J Carson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Phaeoannellomyces McGinnis et Schell gen. nov., which is based upon P. elegans McGinnis et Schell sp. nov., is proposed for dematiaceous yeasts which produce percurrently proliferating conidiogenous cells. Cladosporium werneckii Horta is transferred to Phaeoannellomyces as P. werneckii (Horta) McGinnis et Schell comb. nov. because the most stable and distinctive synanamorph produced by this fungus consists of annellidic yeast cells. The Phaeococcomycetaceae McGinnis et Schell fam. nov. is proposed in the class Blastomycetes, division Fungi Imperfecti for the dematiaceous yeast genera Phaeoannellomyces and Phaeococcomyces de Hoog.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 3","pages":"179-88"},"PeriodicalIF":0.0,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15153579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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