{"title":"Corneal transplant infection by Paecilomyces lilacinus.","authors":"M A Gordon, S W Norton","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Paecilomyces lilacinus was cultured from an ulcerated corneal transplant, sections of which, following surgical removal, revealed dense mycelial growth throughout the corneal remnant and penetrating Descemet's membrane. PAS-stained sections showed, in addition to the hyphae, numerous small, ovoid elements consistent with conidia of P. lilacinus. Infection apparently had spread to the transplant from the recipient's cornea. The fungal isolate proved resistant, in vitro, to amphotericin B, 5-fluorocytosine, and pimaricin, but sensitive to ketoconazole and moderately sensitive to miconazole. Following a second transplant and intensive antifungal therapy, the infection appears to have been eliminated.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 4","pages":"295-301"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15015428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of ELISA-inhibition to Aspergillus antigen standardization for immunodiagnosis.","authors":"J Martínez, A Nieto, J Vives, J M Torres","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An inhibition ELISA method was used to test culture filtrate antigens of Aspergillus fumigatus for the avidity of antibodies to them. Four different culture media were used to check the ability of this assay to distinguish between similar antigenic preparations. Differences between antigens exhibiting similar crossed immunoelectrophoresis patterns were observed by inhibition ELISA.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 4","pages":"317-20"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15016102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methods for reducing particle concentrations of Aspergillus fumigatus conidia and mouldy hay dust.","authors":"J H Edwards, D M Trotman, O F Mason","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effectiveness of commercially available domestic air purifiers to reduce airborne Aspergillus fumigatus conidia and mouldy hay dust was investigated. It was found that the rate of particle clearance is a function of the volume of air passing through the purifiers but that the low throughflow of air makes their use of little value in clearing particles from a normal sized room. Vacuum cleaners were more effective than air purifiers because of their higher air throughput, so too were high volume fan systems in conjunction with simple filtration units. Ionizers had no effect but steam condensation was very efficient at clearing airborne particles.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 4","pages":"237-43"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15015425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SabouraudiaPub Date : 1985-08-01DOI: 10.1080/00362178585380431
P Talwar, B Kumar, A Ayyagiri, S Kaur
{"title":"Prevalence of bacteria and fungi in athlete's foot of varying severity and response to topical antibacterial and antifungal therapies.","authors":"P Talwar, B Kumar, A Ayyagiri, S Kaur","doi":"10.1080/00362178585380431","DOIUrl":"https://doi.org/10.1080/00362178585380431","url":null,"abstract":"<p><p>Ninety-six patients with clinical evidence of interdigital lesions classified as mild, moderate and severe athlete's foot were investigated for bacterial and fungal populations in the interspaces. Gram-negative bacteria, which were not found in the toe spaces of 50 normal controls, were grown in increasing numbers and with increasing frequency as the symptoms progressed from mild to severe. Gram-positive bacteria were also isolated regularly and in increasing numbers commensurate with the severity of the disease. Similarly the isolation rates of dermatophytes and Candida species were higher in patients with moderate and severe disease compared to those with mild disease. Clinical and culture responses to topical applications with framycetin, tolnaftate, miconazole and clotrimazole were also studied. In some patients the prevalence of pathogenic fungi increased as bacterial numbers decreased. The pure antibacterial framycetin brought symptomatic relief, as did the purely anti-dermatophyte substance tolnaftate, but best results were seen with two azole compounds having mixed antibacterial and antifungal properties.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 4","pages":"303-12"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15160832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protection against systemic infections with various Candida species elicited by vaccination with Candida albicans ribosomes.","authors":"E Segal, S Nussbaum, L Barr-Nea","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study investigated whether subcutaneous vaccination of mice with ribosomes from Candida albicans strain CBS 562 would also provide protection against infections by other isolates of Candida. Experiments with a total of 628 mice demonstrated that vaccination induced significant protection against heterologous C. albicans (serotypes A and B) and C. tropicalis isolates in terms of their 30 day survival rates. In all instances, however, protection was lower than that obtained against the homologous strain. In addition, a significant decrease in fungal colonization of the kidneys was found in immunized animals as compared to the non immunized controls. Cell-mediated immune responses against cytoplasmic extracts of the various fungi, as detected in vivo by the foot pad swelling test and in vitro by the lymphocyte transformation assay, were induced by the C. albicans ribosomal vaccination. The results show it is possible to induce cross protection to various Candida species by immunization with C. albicans ribosomes.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 4","pages":"275-85"},"PeriodicalIF":0.0,"publicationDate":"1985-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15015427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SabouraudiaPub Date : 1985-07-01DOI: 10.1080/00362178585380361
J. Edwards, D. Trotman, O. F. Mason
{"title":"Methods for reducing particle concentrations of Aspergillus fumigatus conidia and mouldy hay dust.","authors":"J. Edwards, D. Trotman, O. F. Mason","doi":"10.1080/00362178585380361","DOIUrl":"https://doi.org/10.1080/00362178585380361","url":null,"abstract":"The effectiveness of commercially available domestic air purifiers to reduce airborne Aspergillus fumigatus conidia and mouldy hay dust was investigated. It was found that the rate of particle clearance is a function of the volume of air passing through the purifiers but that the low throughflow of air makes their use of little value in clearing particles from a normal sized room. Vacuum cleaners were more effective than air purifiers because of their higher air throughput, so too were high volume fan systems in conjunction with simple filtration units. Ionizers had no effect but steam condensation was very efficient at clearing airborne particles.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"291 1","pages":"237-43"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88969344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SabouraudiaPub Date : 1985-07-01DOI: 10.1080/00362178585380401
E. Segal, S. Nussbaum, L. Barr-Nea
{"title":"Protection against systemic infections with various Candida species elicited by vaccination with Candida albicans ribosomes.","authors":"E. Segal, S. Nussbaum, L. Barr-Nea","doi":"10.1080/00362178585380401","DOIUrl":"https://doi.org/10.1080/00362178585380401","url":null,"abstract":"This study investigated whether subcutaneous vaccination of mice with ribosomes from Candida albicans strain CBS 562 would also provide protection against infections by other isolates of Candida. Experiments with a total of 628 mice demonstrated that vaccination induced significant protection against heterologous C. albicans (serotypes A and B) and C. tropicalis isolates in terms of their 30 day survival rates. In all instances, however, protection was lower than that obtained against the homologous strain. In addition, a significant decrease in fungal colonization of the kidneys was found in immunized animals as compared to the non immunized controls. Cell-mediated immune responses against cytoplasmic extracts of the various fungi, as detected in vivo by the foot pad swelling test and in vitro by the lymphocyte transformation assay, were induced by the C. albicans ribosomal vaccination. The results show it is possible to induce cross protection to various Candida species by immunization with C. albicans ribosomes.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"167 1","pages":"275-85"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90644380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SabouraudiaPub Date : 1985-07-01DOI: 10.1080/00362178585380411
I. Weitzman, S. Rosenthal, J. Shupack
{"title":"A comparison between Dactylaria gallopava and Scolecobasidium humicola: first report of an infection in a tortoise caused by S. humicola.","authors":"I. Weitzman, S. Rosenthal, J. Shupack","doi":"10.1080/00362178585380411","DOIUrl":"https://doi.org/10.1080/00362178585380411","url":null,"abstract":"Scolecobasidium humicola, a soil fungus and etiologic agent of phaeohyphomycosis in fish, is herein reported to cause cutaneous lesions in a tortoise, Terrapine carolina var. carolina. S. humicola was isolated from lesions on the foot and dematiaceous hyphae were observed in KOH preparations of the biopsy and in stained preparations. This isolate and others were compared morphologically and physiologically with isolates of Dactylaria gallopava which it resembles. As a result of this investigation, we concluded that D. gallopava may be differentiated from S. humicola macroscopically, by the production in D. gallopava of an extensive diffusible purplish-red to reddish-brown pigment when cultured on Sabouraud dextrose agar; microscopically, by the presence and usually predominance of conidia, whose apical cell is markedly wider than the basal cell, and usually constricted at the septum; and physiologically, by the ability to grow on media containing cycloheximide and by the ability to grow well at 36-45 degrees C. In contrast, S. humicola does not usually produce a diffusible pigment on Sabouraud's dextrose agar or if present, is not extensive; it lacks the wider upper cell; is less constricted or non-constricted at the central septum; grows on media containing cycloheximide, although some inhibition may occur and lastly, does not grow at 36 degrees C or higher. Both species were urease positive, hydrolysed tyrosine but not casein, xanthine, or gelatin.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"91 1","pages":"287-93"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80577519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SabouraudiaPub Date : 1985-07-01DOI: 10.1080/00362178585380451
J. Martinez, A. Nieto, J. Vives, J. M. Torres
{"title":"Application of ELISA-inhibition to Aspergillus antigen standardization for immunodiagnosis.","authors":"J. Martinez, A. Nieto, J. Vives, J. M. Torres","doi":"10.1080/00362178585380451","DOIUrl":"https://doi.org/10.1080/00362178585380451","url":null,"abstract":"An inhibition ELISA method was used to test culture filtrate antigens of Aspergillus fumigatus for the avidity of antibodies to them. Four different culture media were used to check the ability of this assay to distinguish between similar antigenic preparations. Differences between antigens exhibiting similar crossed immunoelectrophoresis patterns were observed by inhibition ELISA.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"71 1","pages":"317-20"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81673142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SabouraudiaPub Date : 1985-07-01DOI: 10.1080/00362178585380371
M. Goihman‐Yahr, A. Rothenberg, R. Rosquete, E. Avila-Millán, M. C. de Albornoz, M. H. de Gómez, B. San Martín, A. Ocanto, J. Pereira, T. Molina
{"title":"A novel method for estimating killing ability and digestion of Paracoccidioides brasiliensis by phagocytic cells in vitro.","authors":"M. Goihman‐Yahr, A. Rothenberg, R. Rosquete, E. Avila-Millán, M. C. de Albornoz, M. H. de Gómez, B. San Martín, A. Ocanto, J. Pereira, T. Molina","doi":"10.1080/00362178585380371","DOIUrl":"https://doi.org/10.1080/00362178585380371","url":null,"abstract":"We describe a novel method by which phagocytosis, digestion and killing of Paracoccidioides brasiliensis yeast cells by polymorphonuclear leukocytes or other phagocytic cells may be estimated simultaneously. Suspensions of P. brasiliensis (yeast-like phase) were sonicated, counted and incubated at 37 degrees C with known numbers of phagocytes. Control preparations contained no phagocytic cells. At given intervals samples were incorporated into gelatin nutrient medium and droplets of the mixtures were incubated at room temperature. Live yeast-like P. brasiliensis germinate in vitro and produce filaments. After incubation, droplets may be melted and examined under phase contrast optics, or the cells may be washed and stained by a variation of Papanicolaou's method. Digested P. brasiliensis, intact but non-germinating yeasts and filamented (viable) yeasts may be identified and counted. Killing and digestive abilities of phagocytes may be estimated by the difference between values obtained from phagocyte-containing and control preparations.","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"386 1","pages":"245-51"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84986459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}