Russian Journal of Genetics最新文献

{"title":"Structure of Subpopulations of Ayrshire, Holstein, and Jersey Cattle in the Southern Regions of Russia by CSN2 (rs43703011), CSN3 (rs43703016), and Microsatellite Loci (BM1818, BM1824, BM2113, ETH10, ETH225, ETH3, INRA023, SPS115, TGLA126, TGLA122, TGLA227, TGLA53)","authors":"N. V. Kovalyuk, A. E. Volchenko, E. V. Shiryaeva, L. I. Yakusheva, Yu. Yu. Shakhnazarova","doi":"10.1134/s1022795424030074","DOIUrl":"https://doi.org/10.1134/s1022795424030074","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>During studies using molecular biological methods, 3000 cow blood samples of the Ayrshire, Holstein, and Jersey breeds from the herds of Krasnodar and Stavropol krais were genotyped by the loci <i>BM1818</i>, <i>BM1824</i>, <i>BM2113</i>, <i>ETH10</i>, <i>ETH225</i>, <i>ETH3</i>, <i>INRA023</i>, <i>SPS115</i>, <i>TGLA126</i>, <i>TGLA122</i>, <i>TGLA227</i>, <i>TGLA53</i>, <i>CSN2</i> (rs43703011), and <i>CSN3</i> (rs43703016). On average, the frequency of occurrence of homozygous genotypes for 12 microsatellite loci in Jersey cattle was 0.38; in Holstein and Ayrshire cattle, it was 0.30 and 0.27, respectively; the highest allelic diversity was found in Holstein cattle (9.0 ± 1.7 alleles per locus). It should be noted that there are significant differences in the genetic profile of Ayrshire cattle. For Ayrshire animals, the following allelic variants turned out to be typical (but rare in Jersey and Holstein cattle): 270 of the locus <i>BM1818</i>, 137 of the locus <i>BM2113</i>, 213 of the locus <i>ETH10</i>, 119 of the locus <i>ETH3</i>, 142 of the locus <i>ETH225</i>, 87 of the locus <i>TGLA227</i>, 166 of the locus <i>TGLA53</i>. In the studied subpopulation of Jersey cattle, the frequency of occurrence of the <i>A2A2</i> genotype of the <i>CSN2</i> locus (rs43703011) (beta casein) was 0.68, and in the Ayrshire and Holstein groups, it was only 0.25 and 0.31, respectively. The <i>BB</i> genotype of the <i>CSN3</i> locus (rs43703016) (casein kappa) was found in the Jersey group of animals with a frequency of 0.76, and in Ayrshires and Holsteins, it was 0.04 and 0.15, respectively. The genotyped groups of Jersey, Ayrshire, and Holstein animals are significant in volume; we assume that the identified patterns are typical of cattle of these breeds in the south of Russia. We believe that the data obtained can be used in the future in practical work on classifying certain groups of livestock as typical Holstein, Ayrshire, or Jersey and when creating herds that produce milk of a certain type (for example, type A2 milk).</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"13 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability of Genetic Diversity of Spring Barley by Hordein-Coding Locus over a 40-Year Period of Scientific Breeding in Krasnoyarsk Krai","authors":"N. A. Surin, L. N. Shevtsova, N. S. Kozulina, Yu. M. Borisov","doi":"10.1134/s102279542403013x","DOIUrl":"https://doi.org/10.1134/s102279542403013x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The article discusses and summarizes the results of studies of polymorphism of hordeins (HRD) of spring barley by electrophoresis at the Krasnoyarsk Research Institute of Agriculture for the period from 1988 to 2021. The analysis of the dynamics of the genetic diversity of barley varieties from the ecological and breeding nurseries of the Krasnoyarsk Research Institute of Agriculture released and approved for use in Krasnoyarsk krai showed a significant narrowing of the frequency of occurrence and the loss of a number of alleles characteristic of the conditions of Yenisei Siberia and the appearance of rare variants for the Siberian region. On the basis of long-term observations of the composition of hordeins of breeding samples (promising and rejection) created in different areas of breeding, a number of alleles were found to be associated with economically valuable traits.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"49 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Microsatellite Analysis of Zeboid Cattle with Breeds of Bos taurus","authors":"S. V. Beketov, G. R. Svishcheva, V. P. Upelniek, S. A. Senator, S. B. Kuznetsov, E. A. Nikolaeva, Yu. A. Stolpovsky","doi":"10.1134/s1022795424030050","DOIUrl":"https://doi.org/10.1134/s1022795424030050","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Comparative genotyping of a population of zeboid cattle (<i>Bos taurus</i> × <i>Bos indicus</i>) and eight populations of <i>B. taurus</i> breeds, including six domestic breeds (the Kholmogory, Yaroslavl, Red Gorbatov, Kostroma, Tagil, and Gray Ukrainian) and two transboundary European breeds (Holstein and Brown Swiss), has been conducted using 14 STR markers (<i>BM1824</i>, <i>BM2113</i>, <i>CSRM60</i>, <i>CSSM66</i>, <i>ETH3</i>, <i>ETH10</i>, <i>ETH225</i>, <i>ILSTS006</i>, <i>INRA023</i>, <i>SPS115</i>, <i>TGLA53</i>, <i>TGLA122</i>, <i>TGLA126</i>, and <i>TGLA227</i>). A total of 562 individuals were examined. On the basis of the results of the STR analysis, it was found that all population samples of <i>B. taurus</i> were characterized by an average allelic richness <i>A</i>_R = 4.87–6.7 and a fairly high level of genetic variability <i>H</i>_E = 0.69–0.76 with a slight predominance of random matings <i>F</i>_IS = –0.1–0.0. In zeboid cattle, the corresponding indicators were <i>A</i>_R = 5.96, <i>H</i>_E = 0.72, and <i>F</i>_IS = –0.0278. At the same time, differences in the genetic structure of the <i>B. taurus</i> breeds under consideration were largely determined by population demographic fluctuations, and the genetic and the phenotypic heterogeneity detected in the population of zeboid cattle were determined by a decrease in the effect of artificial selection.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"55 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Аnalyzing Buckwheat Genotypes Obtained on Selective Media with Zinc In Vitro Using ISSR Markers","authors":"S. A. Borovaya, A. G. Klykov, N. G. Boginskaya","doi":"10.1134/s1022795424030062","DOIUrl":"https://doi.org/10.1134/s1022795424030062","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The paper evaluates four ISSR markers (М1, М2, М7, and М11) for their effectiveness in research on the genetic distances of regenerated <i>Fagopyrum esculentum</i> plants, which were obtained in vitro on selective media with high doses of ZnSO₄·7 H₂O (808–1313 mg/L). The level of polymorphism was determined to be high in the combined sample (74.4%). The obtained specimens might be used in the breeding of new varieties with economically important traits.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"46 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogeography of the Polyploid Complex Bassia prostrata s. l. (Chenopodiaceae) Based on Genome Size Analysis and PCR-RFLP cpDNA","authors":"T. V. Pankova, M. N. Lomonosova, O. V. Vaulin, A. Yu. Korolyuk, E. A. Korolyuk, D. N. Shaulo, B. Osmonali","doi":"10.1134/s1022795424030116","DOIUrl":"https://doi.org/10.1134/s1022795424030116","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The cytogeography of the polyploid complex <i>Bassia prostrata</i> s. l. was studied by flow cytometry (FCM) on material from 39 populations in Armenia, Kazakhstan, and Russia. On the basis of the determination of the DNA content in the nuclei (2C-value), three cytotypes were identified: diploid (<i>2n</i> = 18), tetraploid (2<i>n</i> = 36), and hexaploid (2<i>n</i> = 54). Verification of the ploidy level determined by DNA content was carried out by parallel direct counting of the chromosome number. Most of the studied populations are represented by a single cytotype; in three populations, mixed ploidy is noted, when tetraploids or hexaploids are found along with diploids. The genetic isolation of chloroplast DNA of diploid and polyploid cytotypes was revealed. Presumable variants of the evolutionary relationship of cytotypes are shown on the basis of cpDNA restriction spectra.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"30 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure of the Hybrid Zone between Allied Species of the Common Vole, Microtus arvalis and M. obscurus: Influence of Genetic Factors and Landscape-Geographical Conditions","authors":"L. A. Lavrenchenko, A. R. Gromov, A. A. Martynov, D. S. Kostin, V. A. Komarova, D. M. Krivonogov, E. V. Cherepanova","doi":"10.1134/s1022795424030086","DOIUrl":"https://doi.org/10.1134/s1022795424030086","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Patterns of introgression of several genetic markers across the hybrid zone between allied species of the common vole <i>Microtus arvalis</i> s. str. and <i>M. obscurus</i> was studied in four of its sections: in northwest Nizhny Novgorod oblast, east Vladimir and southwest Nizhny Novgorod oblasts, south Lipetsk oblast, and northwest Voronezh oblast. Analysis of the clinal variability for three molecular genetic markers (<i>cytb</i>, <i>tp53</i>, <i>SMCY11</i>) and for karyotypes showed a structural similarity between the “Vladimir–Nizhny Novgorod,” “Nizhny Novgorod,” and “Voronezh” sections. The maximum width was shown for the <i>cytb</i> cline; the minimum width was shown for the <i>SMCY11</i> cline; the <i>tp53</i> cline and chromosomal cline occupy an intermediate position for this parameter. Furthermore, in these transects, the center of the cline for the <i>cytb</i> is shifted southeastward (into the distribution range of <i>M. obscurus</i>) from the centers of three other clines. The revealed asymmetric introgression of mitochondrial genome from <i>M. arvalis</i> to <i>M. obscurus</i> may be explained by the fact that the hybrid zone was formed as a result of invasion of <i>M. obscurus</i> into the range of <i>M. arvalis</i>. The “Lipetsk” transect differs from the three above-mentioned transects in very narrow clines with nearly coinciding centers. Such characteristics of the “Lipetsk” transect are obviously caused by localization of the hybrid zone in this section along Voronezh River. The obtained results led us to suppose that the structure of the studied hybrid zone is determined mainly by coincidence (or noncoincidence) of its center with local physical barriers.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"319 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development Immune-Related Prognostic Model and LncRNA-miRNA-mRNA ceRNA Network for Cervical Cancer","authors":"H. Xu, J. Zhao, T. Zhang, Y. Gao, C. Shi","doi":"10.1134/s1022795424030165","DOIUrl":"https://doi.org/10.1134/s1022795424030165","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Cervical cancer is a serious threat to women’s health. The aim of this study was to provide new insights into the mechanism of cervical cancer by constructing immune-related prognostic model and ceRNA network. The mRNA and circRNA datasets of cervical cancer were downloaded from NCBI GEO database. Wilcox.test was used to screen the differential immune cells between cervical cancer patients and normal participants. WGCNA was performed for identification immune related genes. A circRNA-lncRNA-mRNA network was constructed and the genes in the network were further screened for genes related to prognosis using survival package in R software. The prognostic risk model was further validated in the TCGA database. Finally, GSEA was performed to investigate the different enrichment pathways between high_risk and low_risk groups. Nine genes (BEX4, CCL14, CCL3, CMPK2, FMOD, GHR, HLF, IGFBP5, PAG1) were selected to construct the prognostic model. Patients in the low_risk group had a significantly better prognosis than those in the high_risk group. hsa_circ_0021727-hsa-miR-133b-PAG1 regulatory axis may participate in the regulatory of cervical cancer. The enrichment pathways to patients in the high-risk group and the low-risk group were different. The results were not validated by in vitro and in vivo experiments. We developed an immune-related prognostic model and lncRNA-miRNA-mRNA ceRNA network, which can predict prognosis and understand the mechanism of cervical cancer.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"51 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Molecular Genetic Factors in Formation of the Clinical Type of Neurofibromatosis Type 2","authors":"K. O. Karandasheva, E. S. Makashova, F. A. Ageeva, K. I. Anoshkin, P. A. Sparber, A. O. Borovikov, P. A. Vasiluev, M. S. Pashchenko, A. S. Tanas, V. V. Strelnikov","doi":"10.1134/s1022795424020054","DOIUrl":"https://doi.org/10.1134/s1022795424020054","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Neurofibromatosis type 2 is a hereditary disease with predisposition to the development of multiple tumors of the central and peripheral nervous system. The disease is characterized by significant variability in the clinical picture; the number of neoplasms, their location, and growth rate largely determine the severity of the course. However, assessing the rate of tumor growth requires the availability of a consistent series of instrumental studies conducted within a certain time range, which is not always available at the time of initial treatment. In this study, on the basis of quantitative (age of onset, age of examination) and qualitative (large number of intracranial tumors, large number of spinal tumors, severity of neurological symptoms, mosaic status of the genetic variant) characteristics, an alternative classification of clinical subtypes of neurofibromatosis type 2 was developed. We have revealed statistically significant differences (<i>p</i>-value = 0.037) in the representation of Halliday prognostic classes between the groups identified using the proposed classification, which allows us to suggest the possibility of integrating this approach into clinical practice.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"39 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Effect of Silica Nanoparticles on MMP9, HAS2, ITGAX, and Additional Key Genes: Implications for Enhanced Wound Healing","authors":"B. Mando, A. Al Athamneh, B. Alkhawaja, C. Shannakian, A. Khaleel","doi":"10.1134/s1022795424020108","DOIUrl":"https://doi.org/10.1134/s1022795424020108","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Nanoparticles are an essential aspect of current scientific evolution, offering several potential applications in a number of fields. Particularly, in the therapeutic field such as in wound healing, the unique physical and chemical properties of silica nanoparticles (SiNPs) have attracted attention to it. In this study, we intend to investigate the relationship between silica nanoparticles and wound healing by analyzing altered gene expression before and after the treatment with (SiNPs), of 40 tissue repair related genes, giving special attention to (Matrix Metalloproteinase-9 (MMP-9), hyaluronan synthase 2 (HAS-2), integrin subunit alpha X (ITGA-X), integrin subunit alpha V (ITGA-V), integrin subunit alpha 5 (ITGA-5), S100 calcium binding protein A4 (S100A4), and vascular endothelial growth factor A (VEGFA)), to study the implications of these findings for better wound healing and to provide better insight into how SiNPs might be used to regenerate tissues and repair wounds. Our results showed that silica nanoparticles can significantly enhance wound healing by upregulating the expression of these key genes involved in the process.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"39 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Identification and Analysis of the CCoAOMT Gene Family Revealed Its Relationship to Pomegranate Seed Hardness","authors":"H. M. Suo, H. H. Ni, L. Hu, F. Y. Yuan, M. W. Zhang, S. M. Zhang","doi":"10.1134/s1022795424020121","DOIUrl":"https://doi.org/10.1134/s1022795424020121","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) has a critical function in the lignin biosynthesis pathway. However, its role in pomegranate is not clear. In this research, we identified 11 members of the <i>CCoAOMT</i> gene family based on pomegranate genome-wide data. The 11 <i>CCoAOMT</i> genes were distributed non-uniformly on chromosomes 2, 3 and 4 of pomegranate. Phylogenetic analysis showed that pomegranate <i>CCoAOMT9</i> and Camellia sinensis <i>CCoAOMT</i> genes clustered into one branch. Promoter cis-acting progenitor analysis revealed a role for the pomegranate <i>CCoAOMT</i> gene in the ABA/MeJA response. The expression of <i>CCoAOMT8</i>, <i>CCoAOMT9</i> and <i>CCoAOMT10</i> was higher in hard-seeded cultivars (‘Hongyushizi’ and ‘Baiyushizi’) than in soft-seeded cultivar (Tunisia) as detected and analyzed by q-PCR. The results suggested that <i>CCoAOMT8</i>, <i>CCoAOMT9</i>, and <i>CCoAOMT10</i> might be related to lignin biosynthesis. The findings of this work provided a basis for further investigation of the role of the pomegranate <i>CCoAOMT</i> gene in lignin biosynthesis.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"40 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}