Cytogeography of the Polyploid Complex Bassia prostrata s. l. (Chenopodiaceae) Based on Genome Size Analysis and PCR-RFLP cpDNA

IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY
T. V. Pankova, M. N. Lomonosova, O. V. Vaulin, A. Yu. Korolyuk, E. A. Korolyuk, D. N. Shaulo, B. Osmonali
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引用次数: 0

Abstract

The cytogeography of the polyploid complex Bassia prostrata s. l. was studied by flow cytometry (FCM) on material from 39 populations in Armenia, Kazakhstan, and Russia. On the basis of the determination of the DNA content in the nuclei (2C-value), three cytotypes were identified: diploid (2n = 18), tetraploid (2n = 36), and hexaploid (2n = 54). Verification of the ploidy level determined by DNA content was carried out by parallel direct counting of the chromosome number. Most of the studied populations are represented by a single cytotype; in three populations, mixed ploidy is noted, when tetraploids or hexaploids are found along with diploids. The genetic isolation of chloroplast DNA of diploid and polyploid cytotypes was revealed. Presumable variants of the evolutionary relationship of cytotypes are shown on the basis of cpDNA restriction spectra.

Abstract Image

基于基因组大小分析和 PCR-RFLP cpDNA 的多倍体复合体 Bassia prostrata s. l. (Chenopodiaceae) 的细胞地理学研究
摘要 通过流式细胞仪(FCM)对来自亚美尼亚、哈萨克斯坦和俄罗斯 39 个种群的材料进行了多倍体复合体 Bassia prostrata s. l.的细胞地理学研究。根据细胞核中 DNA 含量(2C 值)的测定,确定了三种细胞型:二倍体(2n = 18)、四倍体(2n = 36)和六倍体(2n = 54)。根据 DNA 含量确定的倍性水平是通过平行直接计算染色体数目来验证的。所研究的大多数种群都是单一细胞型;在三个种群中发现了混合倍性,即四倍体或六倍体与二倍体同时存在。二倍体和多倍体细胞型叶绿体 DNA 的遗传分离得到了揭示。根据 cpDNA 限制性谱图,推测了细胞型进化关系的变体。
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来源期刊
Russian Journal of Genetics
Russian Journal of Genetics 生物-遗传学
CiteScore
1.00
自引率
33.30%
发文量
126
审稿时长
1 months
期刊介绍: Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.
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