The Open Veterinary Science Journal最新文献_第4页

Full-length sequences of one genotype 4 and three genotype 3 Hepatitis E viruses in fecal samples from domestic swine in Japan. 日本家猪粪便中1个基因4型和3个基因3型戊型肝炎病毒的全序列分析

The Open Veterinary Science Journal Pub Date : 2010-05-30 DOI: 10.2174/1874318801004010011

T. Urayama, S. Sapsutthipas, M. Tsujikawa, A. Yamashita, H. Nishigaki, M. S. Ibrahim, K. Hagiwara, M. Yunoki, T. Yasunaga, Teruhide Yamaguchi, K. Ikuta

{"title":"Full-length sequences of one genotype 4 and three genotype 3 Hepatitis E viruses in fecal samples from domestic swine in Japan.","authors":"T. Urayama, S. Sapsutthipas, M. Tsujikawa, A. Yamashita, H. Nishigaki, M. S. Ibrahim, K. Hagiwara, M. Yunoki, T. Yasunaga, Teruhide Yamaguchi, K. Ikuta","doi":"10.2174/1874318801004010011","DOIUrl":"https://doi.org/10.2174/1874318801004010011","url":null,"abstract":"The Hepatitis E virus (HEV) induces zoonotic infections and causes hepatitis. In Japan, HEV occurs in deer, wild boar and swine, and genotype (G)3 and G4 have been isolated from domestic swine. We previously reported that HEV isolates from a total of 320 swine fecal samples from 32 farms in Japan could be predominantly classified into four clusters: three G3 (G3JP, G3SP and G3US) and one G4 (G4JP). In this study, we performed full-length sequencing of four representative HEVs, one from each of the clusters. We found significant nucleotide variation throughout the sequences within a genotype, but not within each cluster. However, we found few variations at the amino acid level. Most of the highly conserved regions within genotypes were concentrated in the overlapping region of open reading frame (ORF)2 and ORF3, while most of the variable regions were within the ORF1 V region. This region was variable even at the amino acid level. Essentially, this region was highly conserved among G3 clusters, with some more dissimilarities between G3SP and the other two clusters, G3JP and G3US. The regions conserved and variable across genotypes had virtually the same positions as those within genotypes, but were much narrower and wider, respectively. For the latter, ORF1 V and P regions were especially variable. Finally, we focused on the sequence conservation in the region widely used for primer and probe sets to detect HEV infections.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126239168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

In Vitro Microbial Degradation of Abnormal Prions in Central Nervous System from Scrapie Affected Sheep 痒病羊中枢神经系统异常朊病毒体外微生物降解的研究

The Open Veterinary Science Journal Pub Date : 2010-05-27 DOI: 10.2174/1874318801004010020

Hongsheng Huang, J. Spencer, J. Guan

{"title":"In Vitro Microbial Degradation of Abnormal Prions in Central Nervous System from Scrapie Affected Sheep","authors":"Hongsheng Huang, J. Spencer, J. Guan","doi":"10.2174/1874318801004010020","DOIUrl":"https://doi.org/10.2174/1874318801004010020","url":null,"abstract":"Abnormal prion protein (PrP Sc ) is highly resistant to inactivation by conventional chemical and physical means. This study was to determine if microbes from the environment could be used to degrade PrP Sc in central nervous system (CNS) tissues from scrapie positive sheep as measured by Western blot. In the first experiment, the number of microbes in CNS tissue suspended in saline was reduced by autoclaving the suspension at 121°C for 5 minutes. Aliquots of this preparation were then inoculated with additional ovine fecal microbes and controls were not inoculated. The results showed that the addition of microbes increased the degradation of PrP Sc in specimens during incubation at room temperature (RT) or at 60°C, but the reduction was greatest at 60°C. In the second experiment, a separate tissue suspension in saline was prepared from CNS tissue from each of 4 scrapie positive sheep and from each of 4 negative sheep. All specimens contained bacteria and after 90 days of incubation at 60°C, PrP Sc in CNS specimens was degraded beyond the detection limit in tissues from 2 scrapie positive sheep and was partially degraded in the other two specimens. The tissues from scrapie negative sheep were consistently negative for PrP Sc . Analysis of microbial 16S ribosomal DNA indicated that during the 90 day incubation period the microbe population shifted from a predominance of mesophiles to thermophiles, based on guanine-cytosine (GC) content of ribosomal RNA genes. The results in this study suggest that microbes commonly found in sheep carcasses or manure could play a role in the degradation of PrP Sc in CNS tissues during incubation at 60°C.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"117 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121151333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Retroviruses in Wild-Born Semi-Captive East African Sanctuary Chimpanzees (Pan troglodytes schweinfurthii) 东非保护区野生半圈养黑猩猩(Pan troglodytes schweinfurthii)中的逆转录病毒

The Open Veterinary Science Journal Pub Date : 2010-04-01 DOI: 10.2174/1874318801004010006

L. Mugisha, C. Kücherer, H. Ellerbrok, S. Junglen, J. Opuda-Asibo, O. Joseph, G. Pauli, F. Leendertz

{"title":"Retroviruses in Wild-Born Semi-Captive East African Sanctuary Chimpanzees (Pan troglodytes schweinfurthii)","authors":"L. Mugisha, C. Kücherer, H. Ellerbrok, S. Junglen, J. Opuda-Asibo, O. Joseph, G. Pauli, F. Leendertz","doi":"10.2174/1874318801004010006","DOIUrl":"https://doi.org/10.2174/1874318801004010006","url":null,"abstract":"Information on retroviruses infections in great apes is scarce, especially for apes kept in sanctuaries throughout Africa. To investigate the prevalence of retroviruses and possible transmission of different retroviruses originating from chimpanzees of different origin (Uganda, Congo and Rwanda), 38 wild-born captive orphan chimpanzees residing in a sanctuary on Ngamba Island were analyzed for retroviral infections. Samples from sanctuary chimpanzees were analyzed using enzyme-linked immunoassays and polymerase chain reactions (PCR). Viruses were characterized by phylogenetic analysis. All chimpanzees were negative for antibodies against Simian Immunodeficiency Virus (SIV) and Simian T-cell Leukemia Virus (STLV). However, 28/38 (73%) chimpanzees were positive to Simian Foamy Virus (SFV) by analysis of a 425-bp DNA segment obtained by PCR using generic integrase primers homologous to highly conserved portions of the polymerase gene. Phylogenetic analysis of SFV sequences obtained in this study formed four sub clusters within the specific SFV P. t. schweinfurthii clade with significant variability among the new SFVs strains. We provide evidence of on-going cross-transmission of SFV among chimpanzees within the sanctuary mostly likely through horizontal routes. We propose to test all chimpanzees introduced into sanctuaries for retroviral and other infections. This will help avoid the spread also of pathogenic viruses in captive populations.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130860380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

The Expression of apxIVA Gene of Actinobacillus pleuropneumoniae Serotype 5 and Establishment of an Indirect ELISA for Distinguishing Infection and Immunization 胸膜肺炎放线杆菌血清5型apxIVA基因的表达及间接ELISA鉴别感染与免疫的建立

The Open Veterinary Science Journal Pub Date : 2010-01-05 DOI: 10.2174/1874318801004010001

N. Zou, Yulan Lai, Min Li, S. Cao, X. Wen, Yong Huang

{"title":"The Expression of apxIVA Gene of Actinobacillus pleuropneumoniae Serotype 5 and Establishment of an Indirect ELISA for Distinguishing Infection and Immunization","authors":"N. Zou, Yulan Lai, Min Li, S. Cao, X. Wen, Yong Huang","doi":"10.2174/1874318801004010001","DOIUrl":"https://doi.org/10.2174/1874318801004010001","url":null,"abstract":"Actinobacillus pleuropneumoniae is the etiological agent of Porcine pleuropneumonia, which causes severe losses in pig farming. To establish an serological method to detect A. pleuropneumoniae infection, bioinformatics method was utilized to analyzed the sequence of apxIVA gene of A. pleuropneumoniae serotype 5 completed by our lab, a 1152 bp fragment of the N'-terminal of apxIVA gene was amplified and cloned into the prokaryotic expression vector pET-32a(+), a recombinant protein about 62KD was expressed upon isopropy 1-� -D- thiogalactoside (IPTG) induction. The protein could react specifically with antiserum from live A. pleuropneumoniae infection in western-blot. After further purification by Ni-NTA, this protein was used to establish an indirect ELISA to detect A. pleuropneumoniae infection. This method showed high specificity and could react positively with antibodies of live A. pleuropneumoniae infection while negatively with those of inactivated A. pleuropneumoniae immunization. In conclusion, this indirect ELISA could be used to detect A. pleuropneumoniae infection in mice model.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127596514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sequence Variation in Hepatitis E Virus Genotypes 3 and 4 from Swine Fecal Samples in Japan 日本猪粪便中戊型肝炎病毒基因3型和基因4型的序列变异

The Open Veterinary Science Journal Pub Date : 2009-11-03 DOI: 10.2174/1874318800903010068

S. Sapsutthipas, T. Urayama, M. Yamate, M. Tsujikawa, H. Nishigaki, K. Hagiwara, M. Yunoki, H. Yasue, Kunio Sato, K. Ikuta

{"title":"Sequence Variation in Hepatitis E Virus Genotypes 3 and 4 from Swine Fecal Samples in Japan","authors":"S. Sapsutthipas, T. Urayama, M. Yamate, M. Tsujikawa, H. Nishigaki, K. Hagiwara, M. Yunoki, H. Yasue, Kunio Sato, K. Ikuta","doi":"10.2174/1874318800903010068","DOIUrl":"https://doi.org/10.2174/1874318800903010068","url":null,"abstract":"Hepatitis E virus (HEV) is a causative agent for hepatitis. HEV is transmitted via the fecal-oral route through contaminated drinking water and induces zoonotic infections through eating uncooked and undercooked meat of deer, wild boar, and swine. In Japan, genotypes 3 (G3) and 4 (G4) are prevalent in domestic swine. Here, we examined the genetic variation among HEVs derived from swine fecal samples in Japan. A total of 320 samples were collected at 32 commercial farm facilities (1 fecal sample from each of 10 pig houses in individual farms). Viral RNA amplification at open reading frame (ORF) 3 was possible in 159 (49.7%) of the fecal samples. For genotyping, the same samples were subjected to amplification at ORF2 and the resulting amplicons were sequenced. The results revealed that all the HEVs in each farm belonged to the same cluster of G3 and G4: G3JP in 8 farms, G3SP in 4 farms, G3US in 6 farms, and G4JP in 2 farms, unclassified G3 in 2 farms, unable to decide due to a low rate of amplification in 5 farms, and no detection in 5 farms. Interestingly, the HEVs from one farm were more homogeneous than those of the same cluster that was derived from other farms. Thus, the efficiency of farm-to-farm transmission of HEVs is likely to be low and HEV seems to have evolved independently at each farm in Japan.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129909776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Cestodes of Untreated Large Stray Dog Populations in Algeria: A Reservoir for Herbivore and Human Parasitic Diseases 阿尔及利亚未经处理的大型流浪狗种群的寄生虫:草食动物和人类寄生虫病的水库

The Open Veterinary Science Journal Pub Date : 2009-10-06 DOI: 10.2174/1874318800903010064

Bourhane Bentounsi, S. Meradi, A. Ayachi, J. Cabaret

{"title":"Cestodes of Untreated Large Stray Dog Populations in Algeria: A Reservoir for Herbivore and Human Parasitic Diseases","authors":"Bourhane Bentounsi, S. Meradi, A. Ayachi, J. Cabaret","doi":"10.2174/1874318800903010064","DOIUrl":"https://doi.org/10.2174/1874318800903010064","url":null,"abstract":"Taeniids are frequent in dogs of Mediterranean countries and most studies have focused on the zoonotic Echinococcus granulosus, although several other species of cestodes are usually collected. We necropsied 127 stray dogs in two areas of eastern Algeria, in order to characterize the cestode communities and the factors that may structure these communities in conditions where anthelmintic treatments are not interfering with infection. The maximum number of species in one single dog was four, among Echinococcus granulosus, Taenia hydatigena, Taenia pisiformis, Taenia multiceps, Dipylidium caninum, or Mesocestoides lineatus. T. hydatigena was the most prevalent in both areas (over 40%) and E. granulosus had different prevalences (16 versus 42%) in the two areas. The associations between cestode species were studied using pairwise and multivariate methods, the latter being more realistic in case of species associations. E. granulosus was positively associated with T. hydatigena whereas the other species were negatively associated with the group T. hydatigena and E. granulosus. The large use of efficient anthelmintics may modify the structure of these communities. Risk factors were evaluated for each species of cestode. Older dogs harbored more T. hydatigena and less T. multiceps, M. lineatus and D. caninum. The latter was less frequent in rural areas.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128472067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Apoptosis induction by the 5′ NCR of infectious Bursal disease virus. 传染性法氏囊病病毒5′NCR诱导细胞凋亡的研究。

The Open Veterinary Science Journal Pub Date : 2009-08-12 DOI: 10.2174/1874318809003010055

Renmao Li, Hai-Yan Wang, Manfu Zhang

{"title":"Apoptosis induction by the 5′ NCR of infectious Bursal disease virus.","authors":"Renmao Li, Hai-Yan Wang, Manfu Zhang","doi":"10.2174/1874318809003010055","DOIUrl":"https://doi.org/10.2174/1874318809003010055","url":null,"abstract":"Virus invasion and replication can induce apoptosis and play an important role in the life cycle of viruses. To illustrate the relationship between apoptosis induction and the non-coding region (NCR) of infectious bursal disease virus (IBDV), the 5� NCR of Harbin-1 (a very virulent IBDV) segment A was sequentially deleted using PCR and exchanged with the corresponding region of the mild virulent strain Cj801. IBDV mutants were recovered using a reverse genetics system and named H� 35, H� 74, H� 94, and HCA. Apoptosis in chicken embryo bursal cells was then determined by flow cytometry. The results revealed that the IBDV mutants could induce apoptosis, but with varying capability. As the length of the deleted sequence increased, the capacity to induce apoptosis decreased. However, there was no marked difference in apoptosis induction between H� 35 and H� 74. The above results indicate that the NCR may increase the ability to induce apoptosis. Quantitative nested real-time PCR was performed to illustrate the relationship between replication and apoptosis. The result showed an obvious positive correlation for H� 35 and HCA but an independent process for H� 74 and H� 94.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123300074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Treatment of 30 Angular Limb Deformities of the Antebrachium and the Crus in the Dog Using Circular External Fixators 圆形外固定架治疗犬前脚角状肢体畸形30例

The Open Veterinary Science Journal Pub Date : 2009-08-10 DOI: 10.2174/1874318800903010041

G. L. Rovesti, G. Schwarz, P. Bogoni

{"title":"Treatment of 30 Angular Limb Deformities of the Antebrachium and the Crus in the Dog Using Circular External Fixators","authors":"G. L. Rovesti, G. Schwarz, P. Bogoni","doi":"10.2174/1874318800903010041","DOIUrl":"https://doi.org/10.2174/1874318800903010041","url":null,"abstract":"Objective: To evaluate frame constructs, surgical technique, postoperative care, complications, treatment duration, and outcome of circular external fixation (CEF) for treatment of 30 antebrachial and crural angular deformities in dogs. Study Design : Retrospective study. Sample Population: Twenty-seven dogs with limb deformities. Methods: Medical records of dogs that underwent surgery with CEF for angular deformities were examined for kind of deformity, frame configuration, surgical technique, treatment duration, complications and outcome. Results: Thirty angular deformities in twenty-seven dogs were corrected using CEF. The mean time of external fixation was 72 days (median ± SD, 65 ± 27 days). Minor and/or major complications were recorded in 26 out of 30 cases. Functional and cosmetic outcomes were considered excellent in 14 cases (46.2%), good in 14 cases (46.2%), and fair in two cases (6.6%). Radiographic outcomes were considered excellent in 14 cases (46.2%), good in 15 cases (49.5%), and fair in one case (3.3%). Conclusion: Although complications were present in 26 out 30 cases, the functional and radiographic outcomes of circular external fixation was considered excellent or good in 92% of cases treated for limb deformities. Clinical Relevance: CEF is an effective treatment modality for antebrachial and crural angular deformities and bone lengthening in dogs. Progressive correction is required for severe angular deformities and lengthening, thus reducing the risk of vascular and/or neurological damage to soft tissues. Complications are frequent, but can be successfully managed in most instances.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116799593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Hay Mite Communities in Sheep Farms from French and Spanish Basque Regions with regard to Scrapie Infection 法国和西班牙巴斯克地区绵羊农场的干草螨群落与痒病感染的关系

The Open Veterinary Science Journal Pub Date : 2009-05-28 DOI: 10.2174/1874318800903010035

T. Hallas, A. García-Pérez, A. Hurtado, J. Barandika, R. Juste, Jean-Marc Arrantz, S. Minéry, L. Gruner, J. Cabaret

引用次数: 1

So you need a protein - a guide to the production of recombinant proteins. 所以你需要一种蛋白质——一种重组蛋白质生产的指南。

The Open Veterinary Science Journal Pub Date : 2009-04-30 DOI: 10.2174/1874318800903010028

R. Jayaraj, P. Smooker

引用次数: 12