The Open Veterinary Science Journal最新文献_第7页

Sperm DNA Fragmentation in Rams Vaccinated with Miloxan 接种米罗仙的公羊精子DNA断裂

The Open Veterinary Science Journal Pub Date : 2008-01-25 DOI: 10.2174/1874318800802010007

J. Gosálvez, J. M. Vázquez, M. Enciso, J. Fernández, A. Gosálbez, J. Bridle, C. López‐Fernández

引用次数: 13

Effects of Butyrate on the Expression of Insulin-Like Growth Factor Binding Proteins in Bovine Kidney Epithelial Cells 丁酸盐对牛肾上皮细胞胰岛素样生长因子结合蛋白表达的影响

The Open Veterinary Science Journal Pub Date : 2008-01-07 DOI: 10.2174/1874318800802010001

Robert W. Li, Congjun Li

{"title":"Effects of Butyrate on the Expression of Insulin-Like Growth Factor Binding Proteins in Bovine Kidney Epithelial Cells","authors":"Robert W. Li, Congjun Li","doi":"10.2174/1874318800802010001","DOIUrl":"https://doi.org/10.2174/1874318800802010001","url":null,"abstract":"Sodium butyrate induces cell cycle arrest and apoptosis in bovine kidney epithelial cells primarily via down- regulating cell cycle-related gene expression and enhancing expression of pro-apoptotic genes. The insulin-like growth factor (IGF) system plays an essential role in these processes. Understanding of regulation of insulin-like growth factor binding proteins (IGFBPs) by butyrate helps reveal the mechanisms by which butyrate induces many physiological proc- esses. In this study, we investigated effects of butyrate on the expression of insulin-like growth factors (IGF1 and IGF2), as well as their receptors (IGF1R and IGF2R) and binding proteins (IGFBPs), in Madin Darby bovine kidney (MDBK) epithelial cells. Our results demonstrated that IGF1 expression was below detection using real-time RT-PCR whereas ex- pression of IGF2 was significantly up-regulated by butyrate in both mRNA and protein levels. Unlike IGF1R, whose ex- pression remained unchanged, IGF2R was also up-regulated by butyrate. Butyrate significantly enhanced expression of IGFBP3 and IGFBP5, while the expression of IGFBP4 and IGFBP6 was down-regulated. Our results suggested that IGF2, not IGF1, may play a critical role in regulating cell cycle progression and programmed cell death by apoptosis in MDBK cells.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127666296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of a Murine Model for Intranasal and Intraperitoneal Infection by Haemophilus parasuis: Short Report 副猪嗜血杆菌鼻内和腹腔感染小鼠模型的评价:简短报告

The Open Veterinary Science Journal Pub Date : 2007-12-30 DOI: 10.2174/1874318800701010011

A. M. D. L. Fuente, C. G. Martín, S. Martínez, R. Frandoloso, E. Ferri

引用次数: 2

Antibody Response to Mannheimia Haemolytica Leukotoxin in Cattle with Respiratory Tract Disease 呼吸道疾病牛对溶血白毒素的抗体反应

The Open Veterinary Science Journal Pub Date : 2007-10-30 DOI: 10.2174/1874318800701010007

S. Cavirani, S. Taddei, C. S. Cabassi, F. Ghidini, C. Piancastelli, C. F. Flammini

引用次数: 6

Distribution and Propagation of Hepatitis E Virus in Experimentally Infected Swine 戊型肝炎病毒在实验感染猪体内的分布和繁殖

The Open Veterinary Science Journal Pub Date : 2007-10-29 DOI: 10.2174/1874318800701010001

K. Hagiwara, Y. Iwabu, Y. Kanai, T. Miyasho, T. Daidoji, M. Yunoki, M. Tsujikawa, Yuu Ohkubo, H. Yasue, K. Ikuta

引用次数: 10

Slaughtered Cattle as a Source of Rumen Fluid to Evaluate Supplements for In Vitro Degradation of Grass Nuts and Barley Straw 屠宰牛瘤胃液来源对草果和大麦秸秆体外降解补充物的评价

The Open Veterinary Science Journal Pub Date : 2007-02-25 DOI: 10.2174/1874318800802010016

A. Chaudhry

{"title":"Slaughtered Cattle as a Source of Rumen Fluid to Evaluate Supplements for In Vitro Degradation of Grass Nuts and Barley Straw","authors":"A. Chaudhry","doi":"10.2174/1874318800802010016","DOIUrl":"https://doi.org/10.2174/1874318800802010016","url":null,"abstract":"This factorial study examined the potential of slaughtered cattle as a source of rumen fluid (RF) to test the im- pact of 0, 90, 180 g/kg forage (Amount) of 2 supplements (Low protein of 12% =LP; high protein of 25% =HP) on in vi- tro rumen degradation of grass nuts (Grass) and barley straw (Straw) over various times. RF was collected from three slaughtered cattle (Animal) on three separate occasions and buffered under CO2 as inoculum at 39 o C. About 1g ground Straw or Grass and each amount of LP or HP were mixed in separate test tubes with 50 ml inoculum under CO2. The tubes fitted with Bunsen valves were incubated at 0-72h after which the contents were washed and dried to estimate dry matter disappearance (DMD). The DMD were statistically analysed for the main effects of Animal, Forage, Supplement, Amount and their interactions at each incubation but not Animal based interactions. The Forage, Amount and Animal ef- fects were significant (P<0.01) at most incubations whereas Supplement effect was significant at 72h only showing greater DMD for HP than LP (P<0.05). DMD increased with time for all main effects. Grass had greater DMD than Straw at all incubations (P<0.001). DMD increased (P<0.05) with time but not at 0h with increasing Amount and increased DMD was greater from 0 to 90 g/kg than at 90 to 180 g/kg. The animals differed (P<0.05) at all but 0h showing variable responses with the confounding effect of their gender, age, breed, size and pre-slaughter feeding. Apparently slaughtered cattle can be used to obtain RF to evaluate supplements for in vitro forage degradation. However, the selection of suitable donor animals of similar pre-slaughter characteristics would be critical if in vitro studies involving slaughtered animals are to be used to evaluate feed supplements before their use for grazing ruminants.","PeriodicalId":214092,"journal":{"name":"The Open Veterinary Science Journal","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131909267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25