Revista Mexicana de Ingeniería Química最新文献_第5页

Callus induction and phytochemical profiling of Yucca carnerosana (Trel.) McKelvey obtained from in vitro cultures 丝兰愈伤组织诱导及植物化学分析McKelvey从体外培养中获得的

Revista Mexicana de Ingeniería Química Pub Date : 2021-02-15 DOI: 10.24275/RMIQ/BIO2209

Y. López-Ramírez, E. Cabañas-García, C. Areche, G. Trejo-Tapia, E. Pérez-Molphe-Balch, Y. A. Gómez-Aguirre

{"title":"Callus induction and phytochemical profiling of Yucca carnerosana (Trel.) McKelvey obtained from in vitro cultures","authors":"Y. López-Ramírez, E. Cabañas-García, C. Areche, G. Trejo-Tapia, E. Pérez-Molphe-Balch, Y. A. Gómez-Aguirre","doi":"10.24275/RMIQ/BIO2209","DOIUrl":"https://doi.org/10.24275/RMIQ/BIO2209","url":null,"abstract":"It has been demonstrated that some species of the Yucca genus are a source of metabolites with functional properties, as is Yucca carnerosana (Trel.) McKelvey with antifungal activity. This research aimed to induce the formation of callus tissue in Y. carnerosana , to know the growth kinetic, and to analyze the metabolite proﬁle of the formed tissue and plants propagated under in vitro and ex vitro conditions. Callus induction was achieved using Murashige & Skoog (MS) medium supplemented with 4.4 µ M benzyladenine and 4.1 µ M 4-aminotrichloropicolinic acid (Picloram). The growth kinetics of callus tissue was characterized by a latency phase achieved at the second week of culture, followed by an exponential growth until the fourth week. The culture showed a speciﬁc growth rate of 0.0258 d − 1 ; the doubling time was 26.866 days, and the growth index was 5.9091. The metabolite proﬁle was analyzed using Ultra-High-Performance Liquid Chromatography coupled to Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS / MS). The chromatographic and mass spectral analysis allowed the separation and identiﬁcation of 22 compounds in callus tissue, 26 in in vitro plants, and 27 in ex vitro plants. Our results indicate that the callus tissue and the in vitro and ex vitro plants of Y. carnerosana may be a source of metabolites of interest.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77625998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

PRESENTACIÓN

Revista Mexicana de Ingeniería Química Pub Date : 2020-12-16 DOI: 10.33996/revistaingenieria.v3i3.34

D. Villalba

引用次数: 0

Multi alcohols continuous unit for biodiesel production: Design and automation 生物柴油多醇连续装置:设计及自动化

Revista Mexicana de Ingeniería Química Pub Date : 2020-12-16 DOI: 10.24275/rmiq/sim2141

R. Pessoa-e-Silva, L. Moura-Andrade, F. A. Silva-Mota, W. F. Alencar-Borges

引用次数: 1

Molecular docking of oxidases from Pleurotus ostreatus and the activity of those produced by ARS 3526 strain grown in both, submerged and solid-state fermentations 平菇氧化酶的分子对接及ARS 3526菌株在液体发酵和固态发酵中产生氧化酶的活性

Revista Mexicana de Ingeniería Química Pub Date : 2020-12-08 DOI: 10.24275/rmiq/bio2076

L. D. Herrera-Zuñiga, M. González-Palma, G. Díaz-Godínez, D. C. Martínez-Carrera, M. C. Sánchez-Hernández, R. Díaz-Godínez

{"title":"Molecular docking of oxidases from Pleurotus ostreatus and the activity of those produced by ARS 3526 strain grown in both, submerged and solid-state fermentations","authors":"L. D. Herrera-Zuñiga, M. González-Palma, G. Díaz-Godínez, D. C. Martínez-Carrera, M. C. Sánchez-Hernández, R. Díaz-Godínez","doi":"10.24275/rmiq/bio2076","DOIUrl":"https://doi.org/10.24275/rmiq/bio2076","url":null,"abstract":"Pleurotus ostreatus is a basidiomycete fungus capable of producing oxidases involved in the degradation of lignin, such as laccase (Lac), manganese peroxidase (MnP), versatile peroxidase (VP), veratryl alcohol oxidase (VAO) and dye-decolorizing peroxidase (DyP). In this research, the molecular docking showed that the interaction between Mn-ion, ABTS or DMP ligand with the respective oxidases studied were strongly supported by exposed GLU and ASP charged residues H-bonded or hydrophobic-bonded, in most of the complexes, mainly GLU and ASP played a very important role in the union, especially in the presence of the Mn-ion. On the other hand, the growth and activity of such enzymes of Pleurotus ostreatus ARS 3526 grown in both, submerged fermentation (SmF) and solid-state fermentation (SSF) were evaluated. The specific growth rate in SSF was 2.5 times higher than in SmF. The values of activity of Lac, VP and DyP were higher in the SSF, of the VAO activity was similar in both fermentation systems and SmF had the higher MnP activity value in comparison with SSF. This study provides evidence of the enzymatic potential of this fungus and shows the similarities in charged amino acids when used in their catalytic interactions, and the intimate relationship between the enzyme and its substrate.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78254845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Acidogenesis/methanogenesis from acid cheese whey in hybrid-UASB reactors 混合- uasb反应器中酸性奶酪乳清产酸/产甲烷的研究

Revista Mexicana de Ingeniería Química Pub Date : 2020-09-01 DOI: 10.24275/rmiq/ia1420

K. N. Cárdenas-Medina, M.C. Fajardo-Ortiz, B. Schettino-Bermúdez, M. Meraz-Rodríguez, P. Castilla-Hernández

{"title":"Acidogenesis/methanogenesis from acid cheese whey in hybrid-UASB reactors","authors":"K. N. Cárdenas-Medina, M.C. Fajardo-Ortiz, B. Schettino-Bermúdez, M. Meraz-Rodríguez, P. Castilla-Hernández","doi":"10.24275/rmiq/ia1420","DOIUrl":"https://doi.org/10.24275/rmiq/ia1420","url":null,"abstract":"The cheese industry produces residues such as acid cheese whey. The latter has a high organic-matter concentration and other nutrients that are severe environmental contaminants. The aim of this study was to evaluate the acidogenesis / methanogenesis of acid cheese whey with short Hydraulic Retention Times (HRT) in Hybrid-UASB reactors, following the evolution of the organic matter, ammonium, and orthophosphate. The acidogenic reactor was operated at an HRT of 0.5 days, while for the methanogenic reactor, this ranged from 1-2 days; both reactors were operated in series. Results showed that the overall HRT of 1.5 days allowed optimal removal (89-96.8%) with organic loading rates of between 2.5 and 10.7 g COD / L-d, while at 14.2-24.9 g COD / L-d, best results were achieved at 2.5 days of overall HRT. Highest methane productivity ranged from 0.99-1.15 L CH 4 / L reactor -d (0.238-0.308 L CH 4 / g COD degraded ). Ammonium formation was only signiﬁcant in methanogenesis (85 mg / L), while orthophosphates showed no change throughout the entire process. In conclusion, Hybrid-UASB reactors operated at short HRT were suitable for the acidogenesis / methanogenesis of this wastewater. Furthermore, methane can be utilized for energy generation, and ammonium and orthophosphate might be employed for the development of high value-added products. L CH 4 / g DQO degradado ). La formación de amonio únicamente fue signiﬁcativa en metanogénesis (85 mg / L); los ortofosfatos no mostraron cambio a través de todo el proceso. En conclusión los reactores UASB-híbridos operados a cortos TRH fueron adecuados para la acidogénesis / metanogénesis del agua residual ensayada. Además, el metano puede ser usado para la generación de energía; el amonio y ortofosfatos podrían emplearse para el desarrollo de productos de alto valor agregado. Palabras clave : Lactosuero ácido, acidogénesis / metanogénesis, reactor UASB-híbrido, eﬂuentes industriales, nutrientes.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88805863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Dextran synthesis by native sugarcane microorganisms 天然甘蔗微生物合成葡聚糖

Revista Mexicana de Ingeniería Química Pub Date : 2020-09-01 DOI: 10.24275/rmiq/bio1793

J. D. Castilla-Marroquín, R. Hernández-Martínez, H. D. L. Vequia, M. Ríos-Corripio, J. R. Hernández-Rosas, M. Rojas-López, F. Hernández-Rosas, Colegio de Postgraduados

{"title":"Dextran synthesis by native sugarcane microorganisms","authors":"J. D. Castilla-Marroquín, R. Hernández-Martínez, H. D. L. Vequia, M. Ríos-Corripio, J. R. Hernández-Rosas, M. Rojas-López, F. Hernández-Rosas, Colegio de Postgraduados","doi":"10.24275/rmiq/bio1793","DOIUrl":"https://doi.org/10.24275/rmiq/bio1793","url":null,"abstract":"The sugarcane agri-food industry boosts the Mexican economy in producing regions. Basing its relevance in sugar production yields. However, by-products are not widely exploited leaving an opportunity for diversiﬁcation. In this study, three microorganism isolates (A, B, and C) were obtained from sugarcane keﬁr; the morphology of isolates B and C corresponded to the lactic acid bacterial genus Leuconostoc . Thus, we examined the potential for these isolates to produce EPSs, like dextran, a molecule with applications in pharmaceuticals, industrials, and foods. The experiment was performed adjusting the active culture concentration to 1 × 10 6 colony-forming units (CFU) / ml, the culture was maintained at 37°C in agitation at 150 rpm. The obtained EPSs were puriﬁed by ethanol and cold acetone precipitation. The results showed that B and C bacterial isolates had the capacity to produce EPSs (14 g / L for isolate B and 32 g / L for isolate C) after 24 h. Fourier-transform infrared spectroscopy (FT-IR) characterization indicated that the EPS was dextran. Further, the produced biopolymer had high solubility in water, avoided freezing at -4°C, and boiled at 85°C.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77951896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Increase in the mortality of the entomopathogenic fungus Metarhizium anisopliae due to the application of an electric field during conidiation 昆虫病原真菌绿僵菌因在条件作用过程中施加电场而死亡率增加

Revista Mexicana de Ingeniería Química Pub Date : 2020-09-01 DOI: 10.24275/rmiq/bio1601

Divanery Rodriguez-Gomez, L. Garate-Velez, A. L. Morales-Piña, M. Gutiérrez-Rojas, N. Velasco-Alvarez

{"title":"Increase in the mortality of the entomopathogenic fungus Metarhizium anisopliae due to the application of an electric field during conidiation","authors":"Divanery Rodriguez-Gomez, L. Garate-Velez, A. L. Morales-Piña, M. Gutiérrez-Rojas, N. Velasco-Alvarez","doi":"10.24275/rmiq/bio1601","DOIUrl":"https://doi.org/10.24275/rmiq/bio1601","url":null,"abstract":"The e ﬀ ects of an electric ﬁeld on conidia production by Metarhizium anisopliae in solid state culture were determined. A 450-mL electrochemical cell (EC) with titanium ruthenium-oxide coated electrodes and packed with a mixture of rice: corn stubble, was inoculated with 1 × 10 6 conidia (g of initial dry support) − 1 and incubated for 8 days (30 ºC). Four days after starting the culture, a current density of 0.09 mA cm − 2 was applied for 24 h. The moisture kept constant (75%), with and without electric ﬁeld. With electric ﬁeld, conidiation (4.9 × 10 8 ± 3 . 7 × 10 7 conidia (g of initial dry support) − 1 ) did not show statistically signiﬁcant di ﬀ erences, but their viability and germination (67 and 45%, respectively) were lower than in the non-perturbated conidia. Total mortality of Tenebrio molitor larvae provoked by conidia produced in presence of the electric ﬁeld was 40% higher compared with the control. The study showed that the application of electric ﬁeld improving the conidial infectivity. This new approach is susceptible to be optimized to other fungi for biological control with the purpose of improving ﬁeld performance and further investigations on the modiﬁcation of cellular mechanisms by the electric ﬁeld stimuli.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78367204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Partial recovery of MRJP1 protein expressed in Pichia pastoris using chromatographic techniques 利用色谱技术部分恢复毕赤酵母中表达的MRJP1蛋白

Revista Mexicana de Ingeniería Química Pub Date : 2020-07-27 DOI: 10.24275/rmiq/bio1713

A. Robles-Zamora, Daniela Enriquez-Ochoa, M. Ureña-Herrera, J. M. Aguilar-Yañez, Marion E. Brunck, Karla Mayolo-Deloisa

{"title":"Partial recovery of MRJP1 protein expressed in Pichia pastoris using chromatographic techniques","authors":"A. Robles-Zamora, Daniela Enriquez-Ochoa, M. Ureña-Herrera, J. M. Aguilar-Yañez, Marion E. Brunck, Karla Mayolo-Deloisa","doi":"10.24275/rmiq/bio1713","DOIUrl":"https://doi.org/10.24275/rmiq/bio1713","url":null,"abstract":"Major Royal Jelly Protein 1 (MRJP1) is the main protein component of the bee-produced complex mixture royal jelly, which is the only nutrient source for queen bees promoting increased lifespan, body size and fertility. Recombinant production of MRJP1 represents an alternative to direct extraction from royal jelly. Production in Pichia pastoris results in high density biomass, with a supernatant containing high amount of impurities. Various methods have been applied to recover and/or purify MRJP1. Here, exploiting the physicochemical properties of MRJP1, reverse phase chromatography (RPC), size exclusion chromatography (SEC) and ion-exchange chromatography (IEX) were investigated as alternative methods to recover MRJP1 directly from supernatant. All techniques showed a 57-kDa band in SDS-PAGE analysis, corresponding to the size of recombinant MRJP1, with contaminants attributed to culture media. However, SEC coupled to IEX evidenced a single peak in the chromatogram corresponding to MRJP1 which suggest it may be a good protocol to recover recombinant MRJP1 from P. pastoris supernatant. This approach serves as a procedure to identify MRJP1 in fermentation culture of P. pastoris. This is the first report about characterization of IEX-based recovery of recombinant Apis mellifera MRJP1 produced in Pichia pastoris without the use of histidine tags.","PeriodicalId":21335,"journal":{"name":"Revista Mexicana de Ingeniería Química","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78223318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fe de errata

Revista Mexicana de Ingeniería Química Pub Date : 2020-07-24 DOI: 10.15517/ri.v30i2.43123

Marcela Adriana Quirós Garita

引用次数: 0

El boom de los tapabocas tapabocas的繁荣

Revista Mexicana de Ingeniería Química Pub Date : 2020-06-01 DOI: 10.16924/REVINGE.50.7

J. C. Briceño, N. Suarez, Camila Castro, C. Agudelo, L. González, S. Isaza

引用次数: 0