Revista Brasileira de Hematologia e Hemoterapia最新文献

{"title":"FMS-related tyrosine kinase 3 internal tandem duplication (FLT3-ITD): a villain among others","authors":"Vanessa Y.N. de Arruda , Lisa N. Matsuzaki , Maria de Lourdes Chauffaille","doi":"10.1016/j.bjhh.2017.03.001","DOIUrl":"10.1016/j.bjhh.2017.03.001","url":null,"abstract":"","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 283-284"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35434637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is karyotyping still needed in the diagnosis and monitoring of chronic myeloid leukemia?","authors":"Maria de Lourdes L.F. Chauffaille","doi":"10.1016/j.bjhh.2016.12.003","DOIUrl":"10.1016/j.bjhh.2016.12.003","url":null,"abstract":"","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 281-282"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2016.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35434636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative flow cytometric evaluation of CD200, CD123, CD43 and CD52 as a tool for the differential diagnosis of mature B-cell neoplasms","authors":"Elissandra Machado Arlindo ,&nbsp;Natália Aydos Marcondes ,&nbsp;Flavo Beno Fernandes ,&nbsp;Gustavo Adolpho Moreira Faulhaber","doi":"10.1016/j.bjhh.2017.05.002","DOIUrl":"10.1016/j.bjhh.2017.05.002","url":null,"abstract":"<div><h3>Background</h3><p>Distinction between mature B-cell neoplasms can be difficult due to overlapping of immunologic features and clinical manifestations. This study investigated whether quantifying mean fluorescence intensity of four monoclonal antibodies in a flow cytometry panel is useful for the differential diagnosis and characterization of these disorders.</p></div><div><h3>Methods</h3><p>The expressions of CD52, CD200, CD123 and CD43 were analyzed in samples from 124 patients with mature B-cell neoplasms. The quantitative estimation of these antigens was assessed by mean fluorescence intensity.</p></div><div><h3>Results</h3><p>The cases included were 78 chronic lymphocytic leukemias, three atypical chronic lymphocytic leukemias, six marginal zone lymphomas, 11 splenic marginal zone lymphomas, nine lymphoplasmacytic lymphomas, six mantle cell lymphomas, two hairy cell leukemias, two hairy cell leukemias variant, five follicular lymphomas, one Burkitt lymphoma and one diffuse large B-cell lymphoma. The mean fluorescence intensity of CD200 was higher in atypical chronic lymphocytic leukemia, chronic lymphocytic leukemia and hairy cell leukemia cases. CD123 showed higher mean fluorescence intensities in hairy cell leukemia cells. Chronic lymphocytic leukemia, atypical chronic lymphocytic leukemia and mantle cell lymphoma had higher expression of CD43 and all follicular lymphoma cases had very low mean fluorescence intensity values. CD52 expression was consistently positive among all cases.</p></div><div><h3>Conclusion</h3><p>Quantitative evaluation of these markers can be a useful additional tool to better identify some types of mature B-cell neoplasms.</p></div>","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 252-258"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35341113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small cell variant of anaplastic large cell lymphoma with leukemic presentation: a diagnostic challenge","authors":"Tomás Zecchini Barrese ,&nbsp;Carlo Sagramoso ,&nbsp;Francesco Bacci ,&nbsp;Elena Sabattini","doi":"10.1016/j.bjhh.2017.05.009","DOIUrl":"10.1016/j.bjhh.2017.05.009","url":null,"abstract":"","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 269-273"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.05.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35341116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ability of serum ferritin to diagnose iron deficiency anemia in an elderly cohort","authors":"Mansour Babaei,&nbsp;Sajedeh Shafiei,&nbsp;Ali Bijani,&nbsp;Behzad Heidari,&nbsp;Seyed Reza Hosseyni,&nbsp;Mohsen Vakili Sadeghi","doi":"10.1016/j.bjhh.2017.02.002","DOIUrl":"10.1016/j.bjhh.2017.02.002","url":null,"abstract":"<div><h3>Background</h3><p>Diagnosis and treatment of iron deficiency anemia in older subjects improves their quality of life. Serum ferritin as a marker of iron stores is an acute phase protein. In older subjects who usually have many concomitant chronic medical conditions, serum ferritin may increase in response to inflammatory processes irrespective of iron stores. This study was performed to determine the diagnostic properties of serum ferritin in the diagnosis of iron deficiency anemia in older subjects.</p></div><div><h3>Methods</h3><p>This case–control study included all the inhabitants of Amirkola town who participated in the Amirkola Health and Aging Project. Diagnosis of anemia was confirmed based on a hemoglobin level &lt;13<!--> <!-->g/dL in men and &lt;12<!--> <!-->g/dL in women and iron deficiency anemia by percent transferrin saturation &lt;15%. A receiver operating characteristic curve was constructed to determine an optimal serum ferritin cutoff value to differentiate patients with and without iron deficiency anemia at the highest sensitivity and specificity.</p></div><div><h3>Results</h3><p>Eighty patients with iron deficiency anemia and 160 cases of anemia without iron deficiency (mean age: 72.9<!--> <!-->±<!--> <!-->8 and 71.6<!--> <!-->±<!--> <!-->7.6 years, respectively; <em>p</em>-value<!--> <!-->=<!--> <!-->0.37) were analyzed. In iron deficiency anemia, the mean serum ferritin was significantly lower (<em>p</em>-value<!--> <!-->=<!--> <!-->0.036) compared to patients without iron deficiency anemia. Serum ferritin with a cutoff level of 100<!--> <!-->ng/mL differentiated patients with and without iron deficiency anemia with a sensitivity of 60% and specificity of 59% and area under the receiver operating characteristic curve of 0.615<!--> <!-->±<!--> <!-->0.040 (95% confidence interval: 0.536–0.694; <em>p</em>-value<!--> <!-->=<!--> <!-->0.004).</p></div><div><h3>Conclusion</h3><p>These findings indicate that in elderly subjects, iron deficiency anemia may develop with higher levels of serum ferritin. Hence, the conventional cutoff of serum ferritin for the diagnosis of iron deficiency anemia in young adults is not appropriate for the elderly population.</p></div>","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 223-228"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35341110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thrombin generation assays for global evaluation of the hemostatic system: perspectives and limitations","authors":"Rita Carolina Figueiredo Duarte ,&nbsp;Cláudia Natália Ferreira ,&nbsp;Danyelle Romana Alves Rios ,&nbsp;Helton José dos Reis ,&nbsp;Maria das Graças Carvalho","doi":"10.1016/j.bjhh.2017.03.009","DOIUrl":"10.1016/j.bjhh.2017.03.009","url":null,"abstract":"<div><p>The existing techniques to evaluate hemostasis in clinical laboratories are not sensitive enough to detect hypercoagulable and mild hypocoagulable states. Under different experimental conditions, the thrombin generation test may meet these requirements. This technique evaluates the overall balance between procoagulant and anticoagulant forces and has provided new insights in our understanding of the coagulation cascade, as well as of the diagnosis of hypocoagulability and hypercoagulability conditions. Thrombin generated in the thrombin generation test can be quantified as platelet-rich or platelet-poor plasma using the calibrated automated thrombogram method, which monitors the cleavage of a fluorogenic substrate that is simultaneously compared to the known thrombin activity in a non-clotting plasma sample. The calibrated automated thrombogram method is an open system, in which different antibodies, proteins, enzymes and peptides can be introduced to answer specific questions regarding hemostatic processes. The thrombin generation test has great clinical potential, such as in monitoring patients taking anticoagulants and antiplatelet drugs, screening for genetic or acquired thrombotic disorders, and evaluating bleeding risk control in patients with hemophilia using bypass agents or replacement therapy. Different to conventional coagulation tests, the thrombin generation test can be used for an overall evaluation of hemostasis, the results of which can then be used to evaluate specific characteristics of hemostasis, such as prothrombin time, activated partial thromboplastin time, and levels of fibrinogen and other coagulation factors. The introduction of this method will contribute to a better understanding and evaluation of overall hemostatic processes; however, this method still requires standardization and clinical validation.</p></div>","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 259-265"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.03.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35434635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular response to imatinib mesylate of Brazilian patients with chronic myeloid leukemia","authors":"Ana Lucia Vieira-Mion,&nbsp;Noemi Farah Pereira,&nbsp;Vaneuza Araujo Moreira Funke,&nbsp;Ricardo Pasquini","doi":"10.1016/j.bjhh.2017.04.007","DOIUrl":"10.1016/j.bjhh.2017.04.007","url":null,"abstract":"<div><h3>Background</h3><p>Imatinib mesylate has revolutionized the treatment of chronic myeloid leukemia leading to significant reductions of <em>BCR-ABL1</em> transcript levels in peripheral blood.</p></div><div><h3>Objective</h3><p>To evaluate the response to imatinib mesylate treatment (400<!--> <!-->mg/day) in Brazilian patients in the chronic phase of chronic myeloid leukemia monitored by quantitative real time polymerase chain reaction.</p></div><div><h3>Methods</h3><p>Between October 2002 and October 2010, 3169 peripheral blood samples were collected from 1403 patients from 3 to 5 months, 6 to 11 months, 12 to 17 months, 18 to 23 months and ≥24 months after beginning imatinib treatment. Eighty-two patients had samples available and analyzed for all time intervals. <em>BCR-ABL1</em> quantification was performed by quantitative real time polymerase chain reaction using the <em>ABL1</em> gene as the control. Results of the <em>BCR-ABL1</em> ratio as a percentage were reported by the international scale (IS) using the laboratory conversion factor (0.51).</p></div><div><h3>Results</h3><p>In the first interval, 80.8% of patients achieved the optimal response (<em>BCR-ABL1</em>^<em>IS</em> <!-->≤<!--> <!-->10%). In the second period, 69.1% achieved optimal response (<em>BCR-ABL1</em>^<em>IS</em> <!-->≤<!--> <!-->1%) and, between 12 and 17 months, 47.3% achieved major molecular response (<em>BCR-ABL1</em>^<em>IS</em> <!-->≤<!--> <!-->0.1%).</p></div><div><h3>Conclusions</h3><p>The results of this retrospective study show that the response to imatinib treatment (400<!--> <!-->mg/day) of Brazilian patients in the chronic phase of chronic myeloid leukemia is within the expected profile when compared to patients reported in international prospective randomized studies.</p></div>","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 3","pages":"Pages 210-215"},"PeriodicalIF":0.0,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.04.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35339669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of atypical beta globin (HBB) gene cluster haplotypes in sickle cell anemia patients of India","authors":"Sandor Rudy L. Nongbri ,&nbsp;Henu Kumar Verma ,&nbsp;Bhaskar V.K.S. Lakkakula ,&nbsp;Pradeep Kumar Patra","doi":"10.1016/j.bjhh.2017.01.007","DOIUrl":"10.1016/j.bjhh.2017.01.007","url":null,"abstract":"","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 2","pages":"Pages 180-182"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.01.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35056632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence, cost per donation and reduction in blood supply due to positive and indeterminate results for infectious markers in a blood bank in Lima, Peru","authors":"Jeel Moya-Salazar ,&nbsp;Roberto Ubidia-Incio ,&nbsp;Maritza Incio-Grande ,&nbsp;Jorgelina L. Blejer ,&nbsp;Carlos A. Gonzalez","doi":"10.1016/j.bjhh.2016.11.007","DOIUrl":"10.1016/j.bjhh.2016.11.007","url":null,"abstract":"<div><h3>Introduction</h3><p>Safety in Transfusion Medicine is subject to regulations and government legislation within a total quality framework. The aim of this study was to evaluate the impact of seroprevalence and indeterminate results on lost units and cost per donation.</p></div><div><h3>Methods</h3><p>A prospective cross-sectional study was performed in the Blood Bank and Transfusion Therapy Department of the Hospital Central de la Policia Nacional del Perú in Lima, Peru. All completed donations (replacement/voluntary) without complications were included in this study. Every donation met the institutional requirements and quality criteria of Programa Nacional de Hemoterapia y Bancos de Sangre (PRONAHEBAS). Data analysis was achieved using the Statistical Package for the Social Sciences.</p></div><div><h3>Results</h3><p>A total of 7723 donations were evaluated during 2014 and 2015 with 493 being seropositive (overall prevalence 5.25%) and 502 having indeterminate results (overall prevalence 5.35%). Thus total loss was 995<!--> <!-->units, 437.8<!--> <!-->L of blood and 49,750 US dollars. The most common seropositive infectious markers were the core antibody of hepatitis B virus (2.82%) and syphilis (1.02%), and the most common indeterminate results were Chagas disease (1.27%) and the core antibody of hepatitis B virus (1.26%). There was no significant change in the prevalence of seropositivity (<em>p</em>-value<!--> <!-->=<!--> <!-->0.243) or indeterminate results (<em>p</em>-value<!--> <!-->=<!--> <!-->0.227) over the two-year period of the study. A statistical correlation was found between the cost per lost donation and the most prevalent markers (rho<!--> <!-->=<!--> <!-->0.848; <em>p</em>-value<!--> <!-->=<!--> <!-->&lt;0.001).</p></div><div><h3>Conclusion</h3><p>Seroprevalence was lower than the regional mean, but the prevalence of indeterminate results was elevated causing a great impact on blood supply and economic losses to this institution.</p></div>","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 2","pages":"Pages 102-107"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2016.11.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35057141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing of human platelet antigens in immune thrombocytopenia patients in northern Brazil","authors":"Julia Cavalcante do Carmo ,&nbsp;Prissyla de Souza Klippel ,&nbsp;Sabrine da Costa Cordeiro ,&nbsp;Ângela Maria dos Santos Fernandes ,&nbsp;Raquel Medeiros Pinto ,&nbsp;Simone Schneider Weber ,&nbsp;Cleiton Fantin","doi":"10.1016/j.bjhh.2017.01.003","DOIUrl":"10.1016/j.bjhh.2017.01.003","url":null,"abstract":"<div><h3>Background</h3><p>Immune thrombocytopenia is an immune disease characterized by thrombocytopenia and bleeding due to platelet antibodies against platelet membrane glycoproteins. Human platelet antigens are derived from polymorphisms of these glycoproteins. The aim of this study was to investigate human platelet antigen frequencies in immune thrombocytopenia patients from the state of Amazonas, Brazil and investigate the potential association between specific antigens and risk for immune thrombocytopenia.</p></div><div><h3>Method</h3><p>Human platelet antigen typing was performed by BeadChip technology to determine allelic variants of 11 systems (HPA-1 to HPA-9, HPA-11 and HPA-15). Thirty-six patients (8 male and 28 female) with a median age of 34 years (range: 9–69 years) were evaluated and compared with data from Amazonas blood donors.</p></div><div><h3>Results</h3><p>Platelet counts varied from 3 to 98<!--> <!-->×<!--> <!-->10⁹/L. The allele frequencies were 0.944 for HPA-1<em>a</em>, 0.056 for HPA-1<em>b</em>, 0.847 for HPA-2<em>a</em>, 0.153 for HPA-2<em>b</em>, 0.555 for HPA-3<em>a</em>, 0.444 for HPA-3<em>b</em>, 0.805 for HPA-5<em>a</em>, 0.222 for HPA-5<em>b</em>, 0.9975 for HPA-9<em>a</em>, 0.025 for HPA-9<em>b</em>, 0.486 for HPA-15<em>a</em> and 0.513 for HPA-15<em>b</em>. Among immune thrombocytopenia individuals, no <em>b</em> allele of the HPA-4, -6, -7, -8 and -11 were found.</p></div><div><h3>Conclusions</h3><p>The results suggest HPA-1a, HPA-3b and HPA-5b are immune thrombocytopenia-specific autoepitopes.</p></div>","PeriodicalId":21233,"journal":{"name":"Revista Brasileira de Hematologia e Hemoterapia","volume":"39 2","pages":"Pages 122-126"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bjhh.2017.01.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35057144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}