Revista De Microbiologia最新文献_第2页

An experimental study of nanoflagellate bacterivory 纳米鞭毛细菌的实验研究

Revista De Microbiologia Pub Date : 1999-12-01 DOI: 10.1590/S0001-37141999000400014

A. Fernandes, H. Mesquita

引用次数: 2

Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by Bacillus sp 果胶溶解活性菌株的筛选:芽孢杆菌产生的聚半乳糖醛酸酶的特性

Revista De Microbiologia Pub Date : 1999-12-01 DOI: 10.1590/S0001-37141999000400002

Marlene Soares, R. Silva, E. Gomes

引用次数: 173

Growth and endoglucanase activity of Acetivibrio cellulolyticus grown in three different cellulosic substrates 三种不同纤维素基质中溶纤维素活性弧菌的生长和内切葡聚糖酶活性

Revista De Microbiologia Pub Date : 1999-12-01 DOI: 10.1590/S0001-37141999000400004

C. Sanchez, C. S. Peres, H. R. Barbosa

引用次数: 10

Candida sp in the oral cavity with and without lesions: maximal inhibitory dilution of Propolis and Periogard 口腔内有无病变的念珠菌:蜂胶和周精的最大抑制稀释

Revista De Microbiologia Pub Date : 1999-12-01 DOI: 10.1590/S0001-37141999000400008

R. V. Azevedo, M. Komesu, R. C. Cândido, C. Salvetti, F. H. C. Rezende

{"title":"Candida sp in the oral cavity with and without lesions: maximal inhibitory dilution of Propolis and Periogard","authors":"R. V. Azevedo, M. Komesu, R. C. Cândido, C. Salvetti, F. H. C. Rezende","doi":"10.1590/S0001-37141999000400008","DOIUrl":"https://doi.org/10.1590/S0001-37141999000400008","url":null,"abstract":"Fifty individuals of both sexes aged on average 45.2 years were evaluated at the Semiology Clinic of FORP-USP in order to isolate and identify yeasts from the oral cavity, with and without lesions, and to determine the maximal inhibitory dilution (MID) of the commercial products Propolis (Apis-Flora) and Periogard (Colgate) against the strains isolated. Yeasts of the genus Candida were detected in the saliva of 9/19 (47.4%) individuals with a clinically healthy mouth, 18/22 (81.8%) of individuals with oral lesions, and in 4/9 (44.4%) of patients with deviation from normality, and were detected in 19/22 (86.4%) of the lesions. In the group with oral candidiasis, we isolated in tongue and lesion, respectively for each specie: C.tropicalis (8% and 10.7%), C.glabrata (4% and 3.6%) and C.parapsilosis (2% and 3.6%), in addition to C.albicans (71.4% and 67.8%) as the only species and the prevalent. The total cfu counts/ml saliva showed a higher mean value in the group with oral candidiasis (171.5% x 103) than in the control group (72.6 x 103) or the group with abnormalities (8.3 x 103). Most of the test strains 67/70 (95.71%) were sensitive to the antiseptics, with Propolis presenting a MID of 1:20 for 54/70/77.1%), and Periogard a MID of 1:160 for 42/70 (60%) strains from healthy sites, results similar to those obtained with strains from oral lesions. Different results were mainly observed among different species. The results indicate the possibility of using the antiseptics Propolis and Periogard (chlorhexidine) for the prevention and treatment of oral candidiasis.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"21 1","pages":"335-341"},"PeriodicalIF":0.0,"publicationDate":"1999-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77167491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Some enzymatic properties of cholesterol oxidase produced by Brevibacterium sp 短杆菌胆固醇氧化酶的酶学性质

Revista De Microbiologia Pub Date : 1999-12-01 DOI: 10.1590/S0001-37141999000400005

T. Salva, A. M. Liserre, A. Moretto, M. Zullo, Gisleine Ventrucci, T. J. Menezes

{"title":"Some enzymatic properties of cholesterol oxidase produced by Brevibacterium sp","authors":"T. Salva, A. M. Liserre, A. Moretto, M. Zullo, Gisleine Ventrucci, T. J. Menezes","doi":"10.1590/S0001-37141999000400005","DOIUrl":"https://doi.org/10.1590/S0001-37141999000400005","url":null,"abstract":"In this study we determined some properties of the cholesterol oxidase from a Brevibacterium strain isolated from buffalo's milk and identified the cholesterol degradation products by the bacterial cell. A small fraction of the enzyme synthesized by cells cultured in liquid medium for 7days was released into the medium whereas a larger fraction remained bound to the cell membrane. The extraction of this fraction was efficiently accomplished in 1 mM phosphate buffer, pH 7.0, containing 0.7% Triton X-100. The enzyme stability under freezing and at 45oC was improved by addition of 20% glycerol. The optimum temperature and pH for the enzyme activity were 53°C and 7.5, respectively. The only steroidal product from cholesterol oxidation by the microbial cell and by the crude extract of the membrane-bound enzyme was 4-colesten-3-one. Chromatographic analysis showed that minor no steroidal compounds as well as 4-colesten-3-one found in the reaction media arose during fermentation process and were extracted together with the enzyme in the buffer solution. Cholesterol oxidation by the membrane-bound enzyme was a first order reaction type.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"1 1","pages":"315-323"},"PeriodicalIF":0.0,"publicationDate":"1999-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83202269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Pathogenicity characteristics of filamentous fungi strains isolated from processed oat 加工燕麦丝状真菌菌株的致病性特征

Revista De Microbiologia Pub Date : 1999-12-01 DOI: 10.1590/S0001-37141999000400016

E. R. D. Silva, M. Cavalcanti, C. Souza-Motta

引用次数: 8

Quantitative evaluation of acidity tolerance of root nodule bacteria 根瘤细菌耐酸性的定量评价

Revista De Microbiologia Pub Date : 1999-07-01 DOI: 10.1590/S0001-37141999000300004

L. D. Oliveira, Hélio Paracaima de Magalhães

引用次数: 15

Detection of viral particles in feces of young dogs and their relationship with clinical signs 幼犬粪便病毒颗粒的检测及其与临床症状的关系

Revista De Microbiologia Pub Date : 1999-07-01 DOI: 10.1590/S0001-37141999000300009

A. D. Amo, A. Aprea, M. Petruccelli

引用次数: 14

Algae and cyanobacteria on painted surfaces in Southern Brazil 巴西南部涂漆表面上的藻类和蓝藻

Revista De Microbiologia Pub Date : 1999-07-01 DOI: 10.1590/S0001-37141999000300005

P. Gaylarde, C. Gaylarde

{"title":"Algae and cyanobacteria on painted surfaces in Southern Brazil","authors":"P. Gaylarde, C. Gaylarde","doi":"10.1590/S0001-37141999000300005","DOIUrl":"https://doi.org/10.1590/S0001-37141999000300005","url":null,"abstract":"Algas e cianobacterias produzem coloracao nas superficies externas de construcoes e podem causar a sua deterioracao fisico-quimica. Apesar a clima umida do Brasil, nao existe no pais uma literatura sobre este problema. O objetivo deste trabalho foi identificar os microrganismos fototroficos mais importantes nas superficies de construcoes, em areas residenciais, urbanas e rurais do Brasil. Foram avaliados os tipos de algas e cianobacterias presentes em superficies pintadas coloradas, em nove municipios do Brasil localizados entre 19° Sul e 30° Sul. Aproximadamente 63% destes foram celulas simples, ou organismos coloniais. O genero, Synechocystis, foi o organismo que mostrou-se o mais diverso e, frequentemente, compoe a maior parte da biomassa, foi detectado em 63,4% das amostras. Outros organismos frequentemente detectados foram os generos Oscillatoria e Chlorella. Este ultimo se destacou como o organismo de maior ocorrencia (72,4%). As cianobacterias foram muito comuns, especialmente em locais urbanos, sendo que, nestas amostras, maior que 62% dos organismos detectados pertenceu a este classe. Organismos fototroficos filamentosos foram detectados em menor numero do que os nao filamentosos em todas as amostras.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"11 1","pages":"209-213"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77258293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Flocculation of fine fluorite particles with Corynebacterium xerosis 干燥棒状杆菌对萤石微粒的絮凝作用

Revista De Microbiologia Pub Date : 1999-07-01 DOI: 10.1590/S0001-37141999000300007

S. R. Haas, F. R. Nascimento, I. Schneider, C. Gaylarde

引用次数: 15