Prostaglandins & other lipid mediators最新文献_第6页

Effect of PGE2 on TT cells viability and division PGE2 对 TT 细胞活力和分裂的影响。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-08-08 DOI: 10.1016/j.prostaglandins.2024.106880

Chien-Chen Lu

{"title":"Effect of PGE2 on TT cells viability and division","authors":"Chien-Chen Lu","doi":"10.1016/j.prostaglandins.2024.106880","DOIUrl":"10.1016/j.prostaglandins.2024.106880","url":null,"abstract":"<div><p>Previous studies have shown prostaglandin E2 (PGE2) produced a marked increase in calcitonin secretion in human C-cells derived from medullary thyroid carcinoma. However, it’s unclear whether PGE2 can increase the growth of C cells. In this study, we use TT cells as a C cell model to investigate the effect of PGE2 on the growth of C cells. The results revealed that both PGE2 and arachidonic acid (AA) significantly increased the count of TT cells, whereas indomethacin and Dup697 reduced this count. Notably, an increase in the level of AA was associated with an increase in the number of proliferating TT cells, indicating a dose–response relationship. PGE2 and its receptor agonists (sulprostone and butaprost) enhanced the proliferation of TT cells. By contrast, 17-phenyl-trinor-PGE2 exerted no significant effect on TT cell proliferation, whereas L161982 suppressed it. The positive effect of AA on TT cell proliferation was inhibited by indomethacin, NS398, Dup697 (complete inhibition), and SC560. Both PGE2 and AA increased the level of p-STAT5a. The positive effect of AA on p-STAT5a was completely inhibited by Dup697 but not indomethacin, NS398, or SC560. Treatment with indomethacin or Dup697 alone reduced the level of STAT5a in TT cells. AA increased the level of STAT5a, but this effect was inhibited by indomethacin, NS398, and Dup697. Overall, this study confirms the effect of PGE2 on the proliferation of TT cells. This effect is likely mediated through EP2, EP3, and EP4 receptors and associated with an increase in p-STAT5a level within TT cells.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106880"},"PeriodicalIF":2.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of sumac on cardiovascular risk factors in adults: A systematic review and meta-analysis of randomized controlled trials 苏木对成人心血管风险因素的影响：随机对照试验的系统回顾和荟萃分析。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-08-07 DOI: 10.1016/j.prostaglandins.2024.106879

Mahdi Vajdi , Ibrahim Saeed Gataa , Fatemeh Pourteymour Fard Tabrizi , Negin Nikrad , Ali Hojati , Melika Darzi , Nooshin Noshadi , Mahsa khajeh , Mahdieh Abbasalizad-Farhangi , Beitullah Alipour

{"title":"The effect of sumac on cardiovascular risk factors in adults: A systematic review and meta-analysis of randomized controlled trials","authors":"Mahdi Vajdi , Ibrahim Saeed Gataa , Fatemeh Pourteymour Fard Tabrizi , Negin Nikrad , Ali Hojati , Melika Darzi , Nooshin Noshadi , Mahsa khajeh , Mahdieh Abbasalizad-Farhangi , Beitullah Alipour","doi":"10.1016/j.prostaglandins.2024.106879","DOIUrl":"10.1016/j.prostaglandins.2024.106879","url":null,"abstract":"<div><p>Clinical evidence suggests the beneficial effects of sumac on cardiovascular risk factors. However, these results are controversial. This systematic review and meta-analysis of randomized controlled trials (RCTs) was performed to determine the effect of sumac supplementation on cardiovascular risk factors in adults<strong>.</strong> The PubMed, Embase, Web of Science, and Cochrane databases were searched from inception to 30 December 2023 to identify RCTs that were published in English. Data were presented as weighted mean difference (WMD) and associated 95 % confidence interval (CI). The quality of the included trials was measured using the Cochrane Collaboration's modified risk of bias tool. A pooled analysis of 16 trials showed that sumac consumption led to a significant reduction in fasting blood glucose (WMD: −6.03 mg/dl; 95 % CI: −9.67 to −2.39), hemoglobin A1c (WMD: −0.45 %; 95 % CI: −0.59 to −0.31), triglycerides (WMD: −9.07 mg/dL; 95 % CI: −16.19 to −1.94), low-density lipoprotein cholesterol (WMD: −5.58 mg/dL; 95 % CI: −11.27 to −0.12), BMI (WMD: −0.22 kg/m<sup>2</sup>; 95 % CI: −0.38 to −0.05), weight (WMD: −0.85 kg; 95 % CI: −1.44 to −0.27), waist circumference (WMD: −0.54 cm; 95 % CI: −0.92 to −0.15), and diastolic blood pressure (WMD: −2.72 mmHg; 95 % CI: −4.16 to −1.29). High-density lipoprotein-cholesterol level also increased significantly (WMD: 3.69 mg/dL; 95 % CI: 1.81–5.57). The overall results support possible protective and therapeutic effects of sumac on cardiovascular risk factors in adults. Additional prospective studies are suggested using longer intervention periods and higher supplementation doses to confirm these results.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106879"},"PeriodicalIF":2.5,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetylsalicylic acid inhibition of the lipoxygenase pathway: Implications for HIV prevention 乙酰水杨酸抑制脂氧合酶途径：对预防艾滋病的影响。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-29 DOI: 10.1016/j.prostaglandins.2024.106878

Monika M. Kowatsch , Tanja Winter , Julius Oyugi , Joshua Kimani , Julie Lajoie , Harold M. Aukema , Keith R. Fowke

{"title":"Acetylsalicylic acid inhibition of the lipoxygenase pathway: Implications for HIV prevention","authors":"Monika M. Kowatsch , Tanja Winter , Julius Oyugi , Joshua Kimani , Julie Lajoie , Harold M. Aukema , Keith R. Fowke","doi":"10.1016/j.prostaglandins.2024.106878","DOIUrl":"10.1016/j.prostaglandins.2024.106878","url":null,"abstract":"<div><h3>Background</h3><p>1.5 million new HIV infections occurred in 2021, suggesting new prevention methods are needed. Inflammation increases the risk for HIV acquisition by attracting HIV target cells to the female genital tract (FGT). In a pilot study, acetylsalicylic acid (ASA/Aspirin) decreased the proportion of FGT HIV target cells by 35 %. However, the mechanism remains unknown.</p></div><div><h3>Methods</h3><p>Women from Nairobi, Kenya took low-dose ASA (81 mg) daily for 6-weeks. Free oxylipins in the plasma were quantified by high-performance liquid chromatography-tandem mass spectroscopy.</p></div><div><h3>Results</h3><p>Oxylipins from 9 fatty acid substrates were detected, with more than one analyte from 4 substrates reduced post-ASA. Summary analysis found ASA downregulated cyclooxygenase and lipoxygenase but not cytochrome P450 activity with a lower n-6/n-3 oxylipin profile, reflecting reduced inflammation post-ASA.</p></div><div><h3>Conclusions</h3><p>Inflammation is associated with increased lipoxygenase activity and HIV risk. Our data suggests ASA reduces inflammation through downregulation of oxylipins. Understanding how ASA reduces inflammation may lead to novel HIV prevention approaches.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106878"},"PeriodicalIF":2.5,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of bitter melon (Momordica charantia) on anthropometric indices in adults: A systematic review and meta-analysis of randomized controlled trials 苦瓜（Momordica charantia）对成人人体测量指数的影响：随机对照试验的系统回顾和荟萃分析

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-28 DOI: 10.1016/j.prostaglandins.2024.106877

Yi Zou , Wenjun Zou , Melika Jahangir , Amirreza Haedi

{"title":"The effects of bitter melon (Momordica charantia) on anthropometric indices in adults: A systematic review and meta-analysis of randomized controlled trials","authors":"Yi Zou , Wenjun Zou , Melika Jahangir , Amirreza Haedi","doi":"10.1016/j.prostaglandins.2024.106877","DOIUrl":"10.1016/j.prostaglandins.2024.106877","url":null,"abstract":"<div><p>There is controversial data on the impacts of bitter melon (Momordica charantia) supplementations on anthropometric indices. Thus, we aimed to clarify this role of bitter melon through a systematic review, and meta-analysis of the trials. All clinical trials conducted on the impact of bitter melon on anthropometric indices were published until August 2023 in PubMed, Web of Sciences, Scopus, Embase, and Cochrane Library web databases included. Overall, 10 studies with 448 individuals were included in the meta-analysis. Meta-analysis of 10 trials with 448 participants revealed no significant reductions in body weight (BW) (WMD: 0.04 Kg; 95 %CI: −0.16–0.25; P =0.651), body mass index (BMI) (WMD: −0.18 kg/m2; 95 %CI: −0.43–0.07; P =0.171), waist circumference (WC) (WMD: −0.95 cm; 95 % CI: −3.05–1.16; p =0.372), and percentage of body fat (PBF) (WMD: −0.99; 95 % CI: −2.33–0.35; p =0.141) following bitter melon supplementation. There was no significant impact of bitter melon supplementation on BW, BMI, WC, and PBF. More large-scale and high-quality RCTs are necessary to confirm these results.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106877"},"PeriodicalIF":2.5,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolvins D5 and D1 undergo phase II metabolism by uridine 5′-diphospho-glucuronosyltransferases Resolvins D5 和 D1 通过尿苷-5'-二磷酸-葡萄糖醛酸转移酶进行 II 期代谢。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-20 DOI: 10.1016/j.prostaglandins.2024.106870

Marina S. Nogueira , Stephanie C. Sanchez , Carol E. Milne , Warda Amin , Sarah J. Thomas , Ginger L. Milne

{"title":"Resolvins D5 and D1 undergo phase II metabolism by uridine 5′-diphospho-glucuronosyltransferases","authors":"Marina S. Nogueira , Stephanie C. Sanchez , Carol E. Milne , Warda Amin , Sarah J. Thomas , Ginger L. Milne","doi":"10.1016/j.prostaglandins.2024.106870","DOIUrl":"10.1016/j.prostaglandins.2024.106870","url":null,"abstract":"<div><p>Specialized pro-resolving mediators (SPMs) are oxidized lipid mediators that have been shown to resolve inflammation in cellular and animal models as well as humans. SPMs and their biological precursors are even commercially available as dietary supplements. It has been understood for more than forty years that pro-inflammatory oxidized lipid mediators, including prostaglandins and leukotrienes, are rapidly inactivated via metabolism. Studies on the metabolism of SPMs are, however, limited. Herein, we report that resolvin D5 (RvD5) and resolvin D1 (RvD1), well-studied SPMs, are readily metabolized by human liver microsomes (HLM) to glucuronide conjugated metabolites. We further show that this transformation is catalyzed by specific uridine 5′-diphospho-glucuronosyltransferase (UGT) isoforms. Additionally, we demonstrate that RvD5 and RvD1 metabolism by HLM is influenced by non-steroidal anti-inflammatory drugs (NSAIDs), which can act as UGT inhibitors through cyclooxygenase-independent mechanisms. The results from these studies highlight the importance of considering metabolism, as well as factors that influence metabolic enzymes, when seeking to quantify SPMs in vivo.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106870"},"PeriodicalIF":2.5,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of leptin and adiponectin in the pathogenesis of post-transplant diabetes mellitus 瘦素和脂肪连通素在移植后糖尿病发病机制中的作用。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-18 DOI: 10.1016/j.prostaglandins.2024.106876

Kamila Szumilas , Aleksandra Wilk , Paweł Szumilas , Violetta Dziedziejko , Andrzej Pawlik

{"title":"Role of leptin and adiponectin in the pathogenesis of post-transplant diabetes mellitus","authors":"Kamila Szumilas , Aleksandra Wilk , Paweł Szumilas , Violetta Dziedziejko , Andrzej Pawlik","doi":"10.1016/j.prostaglandins.2024.106876","DOIUrl":"10.1016/j.prostaglandins.2024.106876","url":null,"abstract":"<div><p>Solid organ transplantation is a life-saving treatment for patients with end-stage organ failure, but it poses unique challenges due to metabolic and immunological changes in recipients. One significant complication is post-transplant diabetes mellitus (PTDM), which affects a variety of solid organ recipients. Leptin, a hormone produced by adipose tissue, regulates appetite and affects glucose metabolism. High leptin levels are associated with the development of PTDM, especially in kidney transplant recipients. Adiponectin, another adipokine, increases insulin sensitivity and has anti-diabetic properties. Low adiponectin levels are associated with insulin resistance and increase the risk of PTDM. As the incidence of PTDM increases due to the increased life expectancy among transplant patients, understanding the role of adipokines such as leptin and adiponectin becomes crucial for early detection and treatment. Additional studies on other adipokines may also provide valuable information on the pathogenesis of PTDM.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106876"},"PeriodicalIF":2.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1098882324000704/pdfft?md5=0c4720dfdaf0c63d27af937a3ace1dac&pid=1-s2.0-S1098882324000704-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PGE2 synthesis and signaling in the liver physiology and pathophysiology: An update 肝脏生理和病理生理学中 PGE2 的合成和信号传导：最新进展。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-16 DOI: 10.1016/j.prostaglandins.2024.106875

Erjiao Qiang , Hu Xu

{"title":"PGE2 synthesis and signaling in the liver physiology and pathophysiology: An update","authors":"Erjiao Qiang , Hu Xu","doi":"10.1016/j.prostaglandins.2024.106875","DOIUrl":"10.1016/j.prostaglandins.2024.106875","url":null,"abstract":"<div><p>The liver plays a central role in systemic metabolism and drug degradation. However, it is highly susceptible to damage due to various factors, including metabolic imbalances, excessive alcohol consumption, viral infections, and drug influences. These factors often result in conditions such as fatty liver, hepatitis, and acute or chronic liver injury. Failure to address these injuries could promptly lead to the development of liver cirrhosis and potentially hepatocellular carcinoma (HCC). Prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) is a metabolite of arachidonic acid that belongs to the class of polyunsaturated fatty acids (PUFA) and is synthesized via the cyclooxygenase (COX) pathway. By binding to its G protein coupled receptors (i.e., EP1, EP2, EP3 and EP4), PGE<sub>2</sub> has a wide range of physiological and pathophysiology effects, including pain, inflammation, fever, cardiovascular homeostasis, etc. Recently, emerging studies showed that PGE<sub>2</sub> plays an indispensable role in liver health and disease. This review focus on the research progress of the role of PGE<sub>2</sub> synthase and its receptors in liver physiological and pathophysiological processes and discuss the possibility of developing liver protective drugs targeting the COXs/PGESs/PGE<sub>2</sub>/EPs axis.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106875"},"PeriodicalIF":2.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does flaxseed supplementation affect apo-lipoproteins? A GRADE-assessed systematic review and meta-analysis 亚麻籽补充剂会影响载脂蛋白吗？

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-14 DOI: 10.1016/j.prostaglandins.2024.106872

Sasan Pourbagher Benam , Seyedsaber Mirabdali , Negar Ebrahimi , Maziar Daneshvar , Shima Vahedi , Fateme Abbasifard , Mahsa Rounagh

{"title":"Does flaxseed supplementation affect apo-lipoproteins? A GRADE-assessed systematic review and meta-analysis","authors":"Sasan Pourbagher Benam , Seyedsaber Mirabdali , Negar Ebrahimi , Maziar Daneshvar , Shima Vahedi , Fateme Abbasifard , Mahsa Rounagh","doi":"10.1016/j.prostaglandins.2024.106872","DOIUrl":"10.1016/j.prostaglandins.2024.106872","url":null,"abstract":"<div><p>Several studies indicated the ameliorating effects of flaxseed supplementation on apolipoproteins, although others have conflicting results. Therefore, the present research was conducted in order to accurately and definitively understand the effect of flaxseed on apolipoproteins in adults. All articles published up to Juan 2024 were systematically searched through PubMed, Scopus, Embase, and Web of Science to collect all randomized clinical trials (RCTs). A random effects model was used to measure the combined effect sizes. Also, standardized mean difference (SMD) and 95 % confidence interval (CI) were used to report the combined effect size. Our results showed that flaxseed supplementation significantly reduced apo-BI (SMD: −0.57; 95 % CI: −0.95, −0.19, p = 0.003; <em>I</em><sup>2</sup> = 83.2 %, heterogeneity p < 0.001) and lipo(a) decreased (SMD: −0.34; 95 % CI: −0.59, −0.09, p=0.007; <em>I</em><sup>2</sup>=30.3 %, heterogeneity p=0.197). However, flaxseed did not change apo-AI levels (SMD: −0.37; 95 % CI: −0.87, 0.13, p = 0.146; <em>I</em><sup>2</sup> = 89.2 %, p-heterogeneity < 0.001). This meta-analysis has shown that flaxseed supplementation may have beneficial effects on apolipoproteins. Future high-quality, long-term clinical trials are needed to confirm our results.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106872"},"PeriodicalIF":2.5,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress 大黄黄连解毒汤通过抑制内质网应激，调节脂肪细胞分化和脂质降解，从而改善肥胖症。

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-11 DOI: 10.1016/j.prostaglandins.2024.106874

Long Hao , Mingxi Li , Huan Gu , Ji Li

{"title":"Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress","authors":"Long Hao , Mingxi Li , Huan Gu , Ji Li","doi":"10.1016/j.prostaglandins.2024.106874","DOIUrl":"10.1016/j.prostaglandins.2024.106874","url":null,"abstract":"<div><p>Dahuang Huanglian Xiexin Decoction (DHXD) is the representative clinical formula for treating epigastric oppression. In this study, we aim to explore the effect of DHXD on obesity and attempt to investigate its potential mechanism. 3T3-L1 preadipocytes were differentiated and high-fat diet-induced obese rat model was established. DHXD was used for treatment and tunicamycin, the activator of endoplasmic reticulum (ER) stress, was adopted to investigate the related regulatory mechanism. Cell viability was evaluated using CCK-8 assay. Oil-Red O staining was performed to determine lipid accumulation. Glycerol production and Triglyceride content were measured using their commercial kits. Western blot was conducted to examine the expression of critical proteins. Results indicated that DHXD could greatly reduce intracellular lipid droplets and triglyceride in differentiated 3T3-L1 cells. Moreover, the elevated expression of mature adipocytes markers, PPARγ, aP2, during adipogenesis was decreased by DHXD treatment. In addition, DHXD aggravated the lipolysis in differentiated 3T3-L1 cells, as evidenced by the upregulated ATGL expression and the downregulated HSL expression. Besides, DHXD inhibited endoplasmic reticulum (ER) stress in 3T3-L1 cells. Further experiments indicated that the impacts of DHXD on adipocyte differentiation and lipid degradation were partly abolished by tunicamycin. Finally, DHXD alleviated lipid accumulation and ER stress in obese rats. In conclusion, DHXD ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting ER stress.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106874"},"PeriodicalIF":2.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different lipid sources on performance, blood lipid parameters, immune system activity, and expression of TNFα and TLR4 genes in broiler chickens 不同脂质来源对肉鸡生产性能、血脂参数、免疫系统活性以及 TNFα 和 TLR4 基因表达的影响

IF 2.5 3区生物学

Prostaglandins & other lipid mediators Pub Date : 2024-07-11 DOI: 10.1016/j.prostaglandins.2024.106873

Mojtaba Ghanbari , Seyed Naser Mousavi , Mohammad Chamani

{"title":"Effects of different lipid sources on performance, blood lipid parameters, immune system activity, and expression of TNFα and TLR4 genes in broiler chickens","authors":"Mojtaba Ghanbari , Seyed Naser Mousavi , Mohammad Chamani","doi":"10.1016/j.prostaglandins.2024.106873","DOIUrl":"10.1016/j.prostaglandins.2024.106873","url":null,"abstract":"<div><p>This study aimed to explore the effects of different lipid sources on the performance, blood lipid parameters, immune system activity, and the expression of <em>TNFα</em> and <em>TLR4</em> genes in broiler chickens. A total of 500 one-day-old male chicks of the ROSS 308 commercial strain were allocated into four treatment groups with five replicates each (each replicate comprised of 25 chickens), following a randomized design. The treatments were as follows: (1) a diet incorporating palm oil (PO, a source of saturated fatty acids); (2) a diet incorporating flaxseed oil (FO, a source of omega-3); (3) a diet incorporating soybean oil (SO, a source of omega-6); and (4) a diet incorporating olive oil (OO, a source of omega-9). According to the findings, the broiler chickens exhibited a significant increase in body weight gain (BWG) throughout the study when their diet consisted of unsaturated oils, as opposed to a diet including PO. Conversely, the feed conversion ratio (FCR) significantly decreased (P<0.01). The treatment with FO resulted in the highest percentage of lymphocytes and antibody titers against Newcastle and Gumboro diseases, showing a significant difference compared to the treatment with PO (P<0.01). Moreover, the relative expression of <em>TNFα</em> and <em>TLR4</em> genes was the lowest following the FO treatment, indicating a significant decrease compared to the treatment with PO. Overall, the present findings demonstrated that incorporating omega-3 fatty acids into the diet was more effective in enhancing the growth performance, immune system, and health of broiler chickens.</p></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"174 ","pages":"Article 106873"},"PeriodicalIF":2.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0