Prostaglandins & other lipid mediators最新文献

{"title":"Effect of Menaquinone-7 (MK-7) Supplementation on Anthropometric Measurements, Glycemic Indices, and Lipid Profiles: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","authors":"Omid Nikpayam ,&nbsp;Ali Jafari ,&nbsp;Amirhossein Faghfouri ,&nbsp;Mohammadjavad Pasand ,&nbsp;Pardis Noura ,&nbsp;Marziyeh Najafi ,&nbsp;Golbon Sohrab","doi":"10.1016/j.prostaglandins.2025.106970","DOIUrl":"10.1016/j.prostaglandins.2025.106970","url":null,"abstract":"<div><h3>Background</h3><div>Menaquinone-7 (MK-7) is a type of vitamin K that has a longer half-life and stays in the body for a more extended period compared to other types of vitamin K. Recently, the effects of this vitamin on body weight, glycemic control, and lipid profiles have garnered much attention. <strong>Aim of the review:</strong> This systematic review and meta-analysis were performed to evaluate the effects of MK-7 on anthropometric measurements, glycemic indices, and lipid profiles.</div></div><div><h3>Methods</h3><div>A systematic search via appropriate keywords was conducted in electronic databases including PubMed, Scopus, Web of Science, and Google Scholar up to October 2023 to obtain relevant original articles. The quality of studies was evaluated using the Cochrane Collaboration tool. Six original articles met our criteria and were included in the analysis.</div></div><div><h3>Results</h3><div>Statistical analysis showed that MK-7 had a desirable effect on inulin (SMD= −0.56; 95 % CI: −0.77, −0.36; P = 0.000, I2 = 84 %, P = 0.000), HbA1c (SMD=-0.32; 95 % CI: −0.55, −0.09; P = 0.007, I2 = 86.8 %, P = 0.000), and homeostatic Model Assessment for Insulin Resistance (HOMA-IR) (SMD= −0.56; 95 % CI: −0.76, −0.35; P = 0.000, I2 = 84.3 %, P = 0.000). Additionally, subgroup analysis revealed negligible effects of MK-7 on total cholesterol (TC), insulin, HbA1c, and HOMA-IR in both genders of patients who received ≤ 90 mg MK-7 for less than 12 weeks. However, MK-7 didn’t have any meaningful effect on other factors.</div></div><div><h3>Conclusion</h3><div>Based on the findings of the present systematic review and meta-analysis, MK-7 may have beneficial effects on glycemic control and TC, although further highly qualified original research is needed for a consistent conclusion.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106970"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-sitosterol regulates PTGS1 to inhibit gastric cancer cell proliferation and angiogenesis","authors":"Jindao Wang ,&nbsp;Minghui Zhou ,&nbsp;Qiuli Zhou ,&nbsp;Guangyang Sun ,&nbsp;Yu Zhang ,&nbsp;Feng Tao ,&nbsp;Minfeng Ye","doi":"10.1016/j.prostaglandins.2025.106964","DOIUrl":"10.1016/j.prostaglandins.2025.106964","url":null,"abstract":"<div><h3>Background</h3><div>Gastric cancer (GC) is the third leading culprit of cancer-related deaths around the world. Beta-sitosterol (BS) is an important phytosterol that has been proven to have anti-proliferative effects on GC and other tumors. However, mechanisms and targets of BS in cancer are rarely explored.</div></div><div><h3>Methods</h3><div>In this investigation, the targets of BS in the treatment of GC were analyzed by network pharmacology. Molecular docking and cellular thermal shift assay were introduced to validate the binding relationship between BS and PTGS1. The impacts of BS on GC cell viability, half maximal inhibitory concentration, proliferation ability, apoptosis level, and angiogenesis ability were detected by using Cell Counting Kit-8, clone formation assay, flow cytometry, and angiogenesis experiment, respectively. In addition, the expression levels of angiogenic factors (VEGF, FGF, PAI-1) were detected by using western blot.</div></div><div><h3>Results</h3><div>In this project, through cell experiments, PTGS1 was identified as a protein that directly binds to BS. <em>In vitro</em> cell experiments revealed that BS promoted apoptosis and inhibited GC cell proliferation and angiogenesis. Importantly, treatment with BS attenuated the promoting influence of PTGS1 overexpression on GC cell proliferation and angiogenesis.</div></div><div><h3>Conclusion</h3><div>This investigation highlighted PTGS1 as the target of BS in GC cells. BS can regulate PTGS1 to inhibit GC cell proliferation and angiogenesis, providing new evidence for the potential use of BS as a therapeutic agent for GC.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106964"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epoxyeicosatrienoic acids (EETs): A novel class of second messengers of hormonal functional responses","authors":"Jorge H. Capdevila ,&nbsp;John R. Falck ,&nbsp;Adeniyi Michael Adebesin","doi":"10.1016/j.prostaglandins.2025.106967","DOIUrl":"10.1016/j.prostaglandins.2025.106967","url":null,"abstract":"<div><div>Epoxyeicosatrienoic acids (EETs) are a class of cytochrome P450 (P450) arachidonic acid (AA) metabolites with diverse biological activities including anti-hypertensive, vasodilatory, angiogenic, and anti-inflammatory properties. While their functions as autocrine and paracrine mediators in cardiovascular and renal systems are well established, their mechanism of action and roles in hormonal functional responses are yet to be fully defined. In this review, we highlight extant evidence of their participation in hormonal transmembrane signal transduction leading to the activation of the ERK1/2 or Akt serine/threonine kinases. Based on studies with EGF (Epidermal Growth Factor), VEGF (Vascular Endothelial Growth Factor) and insulin binding to their membrane bound receptors, we propose to include EETs to the inventory of intracellular mediators associated with the functional responses elicited upon selected hormone/receptor interactions.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106967"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay of LDLR, PCSK9, and lncRNA- LASER genes expression in coronary artery disease: Implications for therapeutic interventions","authors":"Tayebe Ghiasvand ,&nbsp;Jamshid Karimi ,&nbsp;Iraj Khodadadi ,&nbsp;Amirhossein Yazdi ,&nbsp;Salman Khazaei ,&nbsp;Zahra Abedi kichi ,&nbsp;Seyed Kianoosh Hosseini","doi":"10.1016/j.prostaglandins.2025.106969","DOIUrl":"10.1016/j.prostaglandins.2025.106969","url":null,"abstract":"<div><h3>Background and purpose</h3><div>Coronary artery disease (CAD) is defined as stenosis of coronary arteries due to atherosclerosis. The etiology of atherosclerosis can be attributed to a disruption in lipid metabolism, specifically cholesterol and low-density lipoprotein cholesterol (LDL-C). PCSK9 is an enzyme that controls the metabolism of LDL-C by degrading the low-density lipoprotein receptor (LDLR), which in turn affects the metabolism of LDL-C. A newly discovered Long Non-coding RNA named <em>LASER</em>, which affects the homeostasis of cholesterol, has been identified through the evaluation of bioinformatics. The objective of this study was to assess the levels of gene expression related to cholesterol balance, specifically <em>LDLR</em>, <em>PCSK9</em>, and <em>LASER</em>, in peripheral blood mononuclear cells (PBMCs) of Iranian CAD patients in comparison to controls.</div></div><div><h3>Experimental approach</h3><div>This case-control study included 49 CAD patients, with 81.63 % receiving statins, compared to 40 control subjects, of whom 40 % received statins. The qRT-PCR was used to analyze the expression levels of <em>LDLR</em>, <em>PCSK9</em>, and <em>LASER</em> in PBMCs. Additionally, the ELISA method was employed to determine the blood concentration of PCSK9.</div></div><div><h3>Findings / results</h3><div>CAD patients demonstrated a significant reduction in PBMC gene expression levels of LDLR (P &lt; 0.01) and a significant rise in gene expression of <em>PCSK9</em> and <em>LASER</em>, as well as blood concentration of PCSK9 (P &lt; 0.05) compared to controls. The gene expression of <em>PCSK9</em> showed a strong positive relationship with <em>LDLR</em> expression in patients (P = 0.0003). Furthermore, a strong correlation was seen between <em>PCSK9</em> and <em>LASER</em>, as well as <em>LASER</em> and <em>LDLR</em> expression (P &lt; 0.0001) in two groups.</div></div><div><h3>Conclusion and implications</h3><div>PCSK9 and LASER are potential therapeutic targets for atherosclerosis-related disorders, including CAD. Given that patients receiving statins were twice that of the control subjects, and the effect of statins on the <em>LDLR</em>, <em>PCSK9</em> and <em>LASER</em>, further research is required to delineate the distinct effects of coronary artery disease conditions and statin usage on the expression of the aforementioned genes.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106969"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of saffron supplementation on liver and kidney function, blood glucose and pressure in patients with diabetes and prediabetes: A grade assessed systematic review and meta-analysis of randomized controlled trials","authors":"Jia-Wei Zhang ,&nbsp;Qing Zhao ,&nbsp;Zhe Li ,&nbsp;Qian Liu ,&nbsp;Sha-Sha Zang ,&nbsp;Sha Liu","doi":"10.1016/j.prostaglandins.2025.106949","DOIUrl":"10.1016/j.prostaglandins.2025.106949","url":null,"abstract":"<div><div>Saffron has been traditionally used for various health benefits, but its effects on biomarkers of liver function, kidney function, and blood pressure in diabetes are not well understood. This meta-analysis aims to evaluate the impact of saffron supplementation on systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood glucose (FBG), liver enzymes (ALT, AST), and kidney function markers (BUN, creatinine) in patients with diabetes and prediabetes. A comprehensive search was conducted across multiple databases to identify randomized controlled trials (RCTs) assessing saffron/crocin supplementation on glycemic control, hepatic and renal function, and blood pressure regulation in patients with diabetes and prediabetes. Data were extracted and analyzed using random effects model to determine the effect sizes and 95 % confidence intervals (CIs) for each biomarker. The GRADE framework was employed to assess the certainty of the evidence for each outcome. Thirteen studies were included in the meta-analysis. Saffron supplementation significantly reduced SBP (SMD = −0.57, 95 % CI: −0.8 to −0.34, p = 0.036) with the high certainty of evidence, FBG (SMD = −0.57, 95 % CI: −0.93 to −0.22, p = 0.001) with the low certainty of evidence, and AST (SMD = −0.49, 95 % CI: −0.97 to −0.00, p = 0.049) with the low certainty of evidence. Other studied biomarkers were not affected significantly by saffron/crocin supplementation. Saffron/crocin supplementation is effective in decreasing AST, SBP, and FBG levels in patients with diabetes and prediabetes. However, it has no significant effect on ALT, renal function, and DBP. Our observed effect sizes on AST, SBP, and FBG are not clinically important.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106949"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of COX inhibitor and arachidonic acid in manipulating obesity and obesity-induced bone resorption markers in obese mice","authors":"Rahima Begum,&nbsp;Sourav Roy,&nbsp;Md. Abdur Rahman Ripon,&nbsp;Mohammad Tohidul Amin,&nbsp;Mohammad Salim Hossain","doi":"10.1016/j.prostaglandins.2025.106971","DOIUrl":"10.1016/j.prostaglandins.2025.106971","url":null,"abstract":"<div><div>Obesity and bone-loss have remained a focus of research. Obesity stimulates adipose tissue expansion and adipocyte hypertrophy, resulting in chronic low-grade inflammation in the adipocytes. This enlarged adipocyte secretes a variety of pro-inflammatory chemicals. Because of their endocrine signaling, these substances indirectly promote osteoclast activity and bone-loss. However, the role of COX-2 signaling in obesity-induced bone resorption gene expression has yet to be investigated. Thus, we conducted this study in the context of obesity, employing a high-fat diet-induced obese mouse model. Obese mice treated with a selective and non-selective COX-2 inhibitor (celecoxib and aspirin), significantly (p &lt; 0.05) reduced adipogenic markers such as body and fat weight, serum lipids, mRNA expression of pro-inflammatory markers (COX-2, TNF-α, IL-6, and MCP-1) in adipose tissue and bone resorption markers (OPG, RANKL, Cathepsin K, and MMP-9) in tibia bone tissue. In addition, arachidonic acid (AA) supplementation significantly (p &lt; 0.5) increased the expression of obesity-induced inflammatory cytokines in the tibia bone marrow via the COX-2-derived PGE₂ signaling pathway, hence increase the osteoclastogenesis. These findings suggested that inhibiting the COX-2 signaling pathway could reduce obesity and inflammatory bone resorption. Although both the selective and non-selective COX inhibitors had similar effects, selective COX-2 was more effective in these events, indicating that COX-2 plays a critical role in obesity-associated inflammatory bone resorption.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106971"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143611011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of curcumin on lipid mediators, glycemic index, and oxidative stress and inflammation biomarkers in polycystic ovary syndrome: Future directions and current knowledge – A systematic review","authors":"Hiba Muwafaq Saleem ,&nbsp;Hussein Riyadh Abdul Kareem Al-Hetty ,&nbsp;Abdulrahman T. Ahmed ,&nbsp;Muthanna M. Awad ,&nbsp;Mohammed Qais Al-Ani ,&nbsp;Mustafa Nuhad Al-Darraji ,&nbsp;Dina Akeel Salman ,&nbsp;Loay H. Ali","doi":"10.1016/j.prostaglandins.2024.106947","DOIUrl":"10.1016/j.prostaglandins.2024.106947","url":null,"abstract":"<div><div>Polycystic ovary syndrome (PCOS) is one of the most common and important polygenic endocrine disorders among women of reproductive-aged. Current treatments are mostly used only to control the signs and symptoms of the disease, while not being able to completely prevent complications. Curcumin is one of the active compounds in turmeric, which is commonly used for a wide range of metabolic and inflammatory diseases. Therefore, this systematic review was performed to evaluate the effect of curcumin supplementation on PCOS. The current systematic review was performed according to the guidelines of the 2015 PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) statements. We searched ProQuest, PubMed, Google Scholar electronic, Scopus, and Cochrane, Embase, and Science Direct databases and on articles published up until November 2024. All of the animal studies (seven studies) and clinical trials (five studies) included in this systematic review that assessed the effect of curcumin on, reproductive hormones and metabolic risk markers in PCOS were published in English-language journals. Most studies supported the beneficial effects of curcumin on folliculogenesis, ovarian histomorphology, and luteinization processes. The effects of curcumin on decreasing the levels of luteinizing insulin resistance luteinizing hormone (LH), Follicle-stimulating hormone (FSH)and testosterone, were also reported. Curcumin also improved dyslipidemia, but no significant effect on weight loss has been reported. It is suggested that the effect of curcumin in PCOS is more related to the antioxidant and anti-inflammatory properties of curcumin than to the effects of weight loss. Therefore, this study provides evidence that curcumin can be considered an effective factor in reducing the complications of PCOS. However, due to the low number of human studies in this field, further clinical trials are warranted to verify these outcomes.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106947"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the serum phospholipid profile of neuroborreliosis patients, foresters, and patients subjected to long-term therapy according to ILADS methods","authors":"Wojciech Łuczaj ,&nbsp;Anna Moniuszko-Malinowska ,&nbsp;Monika Groth ,&nbsp;Elżbieta Skrzydlewska","doi":"10.1016/j.prostaglandins.2025.106966","DOIUrl":"10.1016/j.prostaglandins.2025.106966","url":null,"abstract":"<div><div>The aim of the study was the assessment of changes in the serum phospholipid profile patients with Lyme neuroborreliosis (NB), asymptomatic people after frequent tick bites and patients after long-term multidrug therapy against <em>B.burgdorferi</em>. LC-QTOF-MS/MS platform was used to identify changes in serum phospholipid profile of 37 persons. The results demonstrate differences PL profile among patients with Lyme borreliosis (LB), people frequently exposed to tick bites and patients treated with long-term multidrug therapy compared to healthy subjects. Significant differences in SM, LPC, PI, and PC content of NB patients discriminate this group of patients from the other groups, have potential diagnostic value, and can be used for the development of more effective diagnostic tools. The finding that the phospholipid profile of foresters is similar to healthy people, suggests the existence of adaptive mechanisms that are currently difficult to explain but are interesting from the perspective of future research.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"177 ","pages":"Article 106966"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of Yougui Yin in experimental knee osteoarthritis: From the perspective of macrophage polarization","authors":"Zhongqing Wu ,&nbsp;Kanna Xu ,&nbsp;Minchang Chen ,&nbsp;Shihao Wang ,&nbsp;Yong Ma","doi":"10.1016/j.prostaglandins.2024.106940","DOIUrl":"10.1016/j.prostaglandins.2024.106940","url":null,"abstract":"<div><div>Knee osteoarthritis (KOA) refers to a prevalent musculoskeletal disorder, frequently complicated by substantial pain and physical disability. Yougui Yin (YGY) is a classic Chinese herbal mixture which has demonstrated potential in treating KOA. Considering that, its cryptic mechanism warrants to be deciphered, which is the subject of our present research. In vivo, H&amp;E staining, Alcian blue staining and Masson staining assessed the histomorphology. Commercial kits and ELISA evaluated oxidative stress markers. ELISA also assayed serum inflammatory cytokines. TUNEL staining appraised apoptosis. Western blotting examined cartilage matrix degradation, apoptotic and NLRP3 inflammasome proteins. Immunofluorescence assay estimated macrophage polarization. In vitro, ELISA assayed oxidative stress markers and inflammatory cytokines. Immunofluorescence and flow cytometry assay estimated macrophage polarization. MTT and flow cytometry assays severally measured cell viability and apoptosis. DCFH-DA probe detected ROS formation. RT-qPCR and Western blotting examined chondrocyte markers, apoptotic and pyroptotic genes. YGY significantly eased the histomorphological damage, apoptosis and pyroptosis in the cartilage tissues of KOA mice. Besides, YGY exerted anti-oxidant and anti-inflammatory activities and drove M1-to-M2 polarization of macrophages both in vitro and in vivo. Further, the co-culture of macrophages treated by LPS and serum containing YGY improved the viability, eliminated the apoptosis, pyroptosis, inflammation, oxidative stress and cartilage degradation in TNF-α-exposed chondrocytes co-cultured with LPS-intervened macrophages. Overall, YGY might mediate macrophage polarization to impede the advancement of KOA.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"176 ","pages":"Article 106940"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cigarette smoke-induced attenuation of the prostaglandin transporter SLCO2A1 expression through aryl hydrocarbon receptor","authors":"Melody N. Shumba,&nbsp;Yoshinobu Nakamura,&nbsp;Takeo Nakanishi","doi":"10.1016/j.prostaglandins.2024.106935","DOIUrl":"10.1016/j.prostaglandins.2024.106935","url":null,"abstract":"<div><div>SLCO2A1 is a prostaglandin transporter and contributes to regulating local concentration of an inflammatory mediator, PGE₂. Since we previously found that cigarette smoke extracts (CSE) reduced Slco2a1 mRNA expression in rat alveolar epithelial cells, the current study aimed to investigate the effect of CSE on human SLCO2A1 mRNA expression across cell lines from organs that are susceptible to tobacco smoking-induced inflammation. 5’-Flanking regions of SLCO2A1 up to 3673 bp upstream of the transcription start site (+1) was sub-cloned into a luciferase (LUC) expression vector, and promoter activity was evaluated by a reporter assay. CSE significantly reduced SLCO2A1 mRNA expression and LUC activity driven by the construct of −3673/+4 in colon epithelial LoVo and Caco-2 and lung mucoepidermoid NCI-H292 cells, but not in liver epithelial-like HepG2 cells. Long-term exposure of LoVo cells to CSE completely suppressed SLCO2A1 protein expression. The CSE-mediated effect on LUC activity was restored by an AHR antagonist PD98059 and a known AHR ligand β-naphthoflavone significantly reduced SLCO2A1 mRNA expression in cells. Concomitantly, the CSE-mediated negative regulation of SLCO2A1 was abolished in cells transfected with the construct of −3673/+4 with mutated xenobiotic response element. Furthermore, PD98059 and an AHR inhibitor perillaldehyde diminished the negative effect of CSE on SLCO2A1 mRNA expression in Lovo, NCI-H292 and Caco-2 cells. These results demonstrate that CSE negatively modulates SLCO2A1 transcription through AHR activation, providing a toxicological implication of tobacco smoke-induced inflammation.</div></div>","PeriodicalId":21161,"journal":{"name":"Prostaglandins & other lipid mediators","volume":"176 ","pages":"Article 106935"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}