Potential benefit of Lactobacillus acidophilus supplementation to rats fed with a high-fat diet on serum lipid profile, kidney amyloid protein and tumor necrosis factor-alpha level

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins & other lipid mediators Pub Date : 2024-09-23 DOI:10.1016/j.prostaglandins.2024.106911

Pınar Kar , Gülay Çiftci , Alper Çiftci

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引用次数: 0

Abstract

Aim

It was aimed to determine the potential effect of Lactobacillus acidophilus supplementation on rats exposed to an experimental high-fat diet on serum lipid profile and kidney total beta amyloid protein (TBAP) and Tumor Necrosis Factor-alpha (TNF-α) levels.

Methods

24 male Sprague-Dawley rats were used in the study to establish 4 groups. Standard rat food (SD) was provided to Group 1 as the control; Group 2 was fed a high-fat diet (HFD); Group 3 consumed SD and received L. acidophilus probiotics; Group 4 was fed HFD and received L. acidophilus probiotics. Body weights were determined weekly during the 12-week trial period. At the end of the experiment, TBP and TNF-α levels in the serum and kidney tissue of the rats were measured by ELISA method. Serum total cholesterol (TC), triglyceride (TG), HDL, LDL, urea and creatinine levels and paraoxanase, amylase and lipase activities were determined by spectrophotometric method on the analyzer device.

Results

When the control (Group 1) group and Group 2 were compared at the end of the experiment, it was found that Group 2 had gained the most weight and that both the blood and kidney tissue levels of TNF-α and TBAP, as well as the quantities of TG, TK, LDL, and urea, were significantly greater (P<0.05). Serum HDL, PON and amylase levels were found to be significantly low (P>0.05). TG, TK, LDL, urea, and the levels of TNF-α and TBAP in serum and renal tissue were shown to be lower in the groups who received L. acidophilus probiotics (Groups 3, 4) when compared to Group 2 (P>0.05). It was observed that HDL, PON and amylase levels increased and approached the control group (P<0.05).

Conclusion

The study's findings showed that probiotic supplementation improved blood levels of TG, TC, HDL, LDL, urea, PON, and amylase as well as serum and kidney tissue levels of TNF-α and TBAP in obese rats fed a high-fat diet.

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高脂饮食大鼠补充嗜酸乳杆菌对血清脂质、肾淀粉样蛋白和肿瘤坏死因子-α水平的潜在益处

目的：本研究旨在确定补充嗜酸乳杆菌对暴露于实验性高脂饮食的大鼠血清脂质概况、肾脏总β淀粉样蛋白（TBAP）和肿瘤坏死因子-α（TNF-α）水平的潜在影响。第 1 组以标准大鼠食物（SD）作为对照；第 2 组喂食高脂肪食物（HFD）；第 3 组喂食标准大鼠食物并摄入嗜酸乳杆菌益生菌；第 4 组喂食高脂肪食物并摄入嗜酸乳杆菌益生菌。在为期 12 周的试验期间，每周测定一次体重。实验结束时，采用 ELISA 方法测定大鼠血清和肾组织中的 TBP 和 TNF-α 水平。血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、尿素和肌酐水平，以及副氧杂环戊烷酶、淀粉酶和脂肪酶活性均通过分析仪上的分光光度法进行测定：实验结束后，对比对照组（第1组）和第2组，发现第2组体重增加最多，血液和肾组织中TNF-α和TBAP的含量以及TG、TK、LDL和尿素的含量均显著增加（P0.05）。与第 2 组相比，服用嗜酸乳杆菌益生菌组（第 3、4 组）的 TG、TK、LDL、尿素以及血清和肾组织中 TNF-α 和 TBAP 的含量均较低（P>0.05）。据观察，HDL、PON 和淀粉酶水平有所上升，并接近对照组（PC结论：研究结果表明，补充益生菌可改善以高脂肪饮食喂养的肥胖大鼠血液中的 TG、TC、HDL、LDL、尿素、PON 和淀粉酶水平，以及血清和肾组织中的 TNF-α 和 TBAP 水平。

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来源期刊

Prostaglandins & other lipid mediators 生物-生化与分子生物学

CiteScore

5.80

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Prostaglandins & Other Lipid Mediators is the original and foremost journal dealing with prostaglandins and related lipid mediator substances. It includes basic and clinical studies related to the pharmacology, physiology, pathology and biochemistry of lipid mediators. Prostaglandins & Other Lipid Mediators invites reports of original research, mini-reviews, reviews, and methods articles in the basic and clinical aspects of all areas of lipid mediator research: cell biology, developmental biology, genetics, molecular biology, chemistry, biochemistry, physiology, pharmacology, endocrinology, biology, the medical sciences, and epidemiology. Prostaglandins & Other Lipid Mediators also accepts proposals for special issue topics. The Editors will make every effort to advise authors of the decision on the submitted manuscript within 3-4 weeks of receipt.