Reproduction最新文献

{"title":"Comparison of clinical artificial oocyte activation protocols on mouse egg activation and embryo development.","authors":"Lucas N González, Valeria Sulzyk, Patricia S Cuasnicú, Débora J Cohen","doi":"10.1530/REP-24-0387","DOIUrl":"10.1530/REP-24-0387","url":null,"abstract":"<p><strong>In brief: </strong>Artificial oocyte activation (AOA) with Ca2+ ionophores is a valuable tool in assisted reproduction but may affect cellular events critical for embryo development. This study shows the impact of different AOA protocols on embryonic development efficiency and meiotic progression.</p><p><strong>Abstract: </strong>AOA with Ca2+ ionophores is an experimental procedure that benefits patients who fail to obtain fertilized eggs. However, the impact of non-physiological Ca2+ increases on cellular events involved in the egg-embryo transition and early development remains poorly understood. Using the mouse model, this study compares common Ca2+ ionophore protocols applied in clinical practice - one or two exposures to A23187 or a single exposure to ionomycin - focusing on embryonic development and cellular events associated with egg activation. All groups of ionophore-activated eggs exhibit lower levels of first mitotic division compared to those activated by spermatozoa or SrCl2, attributable to variations in Ca2+ dynamics during activation. At the cellular level, these eggs presented defects in spindle morphology and chromosome segregation during meiosis progression, associated with lower levels of cytoplasmic ATP, without changes in reactive oxygen species. These findings highlight the importance of optimizing Ca2+ management in AOA protocols.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel insight into CD90 mesenchymal stem cell marker in mouse endometrial stroma during embryo implantation.","authors":"Anna O Gaidamaka, Alena D Alexandrova, Elina S Chermnykh, Zakhar R Starinnov, Marat S Sabirov, Yulia Y Silaeva, Ekaterina A Vorotelyak","doi":"10.1530/REP-24-0375","DOIUrl":"10.1530/REP-24-0375","url":null,"abstract":"<p><strong>In brief: </strong>Because the study of endometrial stem or progenitor cells has focused more on their potential ability to repair the endometrium, it is necessary to investigate their role in preparing the tissue for embryo implantation. Therefore, we selected CD90 protein as a marker of the population of cells with progenitor properties to study their contribution to hormone-regulated endometrial processes.</p><p><strong>Abstract: </strong>The endometrium is a dynamic tissue that undergoes significant changes during the reproductive cycle and pregnancy. Its high regenerative capacity is because of the presence of progenitor cells, which maintain tissue homeostasis. Previous studies have identified small populations of endometrial progenitor cells and investigated their role in tissue repair. However, the involvement of these cells in hormone-mediated changes in the endometrial stroma during decidualization and implantation has not been fully understood. In this study, we used CD90 as a potential marker for endometrial progenitor cells. We demonstrated that CD90+ cells have a higher clonogenicity and lower proliferative potential than CD90-. The localization of this marker around the embryo during decidualization led us to hypothesize that CD90+ cells may be directly involved in preparing the endometrial stroma for implantation. The results demonstrated an increase in the percentage of CD90+ cells in the endometrium of pregnant mice compared with nonpregnant mice. Additionally, the embryos exhibited a greater ability to spread on CD90+ cells in vitro. Our data support the hypothesis that CD90+ cells play a functional role in implantation, although the exact hormone-dependent mechanisms require further investigation. Based on the subluminal localization of CD90+ cells, we suggested that the luminal epithelium maintains a CD90+ cell surface phenotype. Consequently, we established epithelial-mesenchymal organoids, and the localization of CD90 in these organoids resembled that observed in vivo. Overall, CD90+ cells demonstrate high clonogenicity and contribute to the preparation of the stroma for interaction with the embryo during the implantation process.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special series on reproductive health in transgender and gender-diverse patients.","authors":"Molly B Moravek, Gene de Haan, Vasantha Padmanabhan","doi":"10.1530/REP-24-0455","DOIUrl":"10.1530/REP-24-0455","url":null,"abstract":"","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia-inducible factor inhibition affects luteal function with no effect on fertility in mice.","authors":"Rocío Celeste Marinoni, María José España De Marco, Candela Velazquez, Katherine Prost, Fernanda Parborell, Marta Tesone, Dalhia Abramovich","doi":"10.1530/REP-24-0313","DOIUrl":"10.1530/REP-24-0313","url":null,"abstract":"<p><strong>In brief: </strong>Formation and function of the corpus luteum strongly rely on active angiogenesis. This study demonstrates the role of the hypoxia-inducible factor (HIF) in luteinization with no effect on fertility.</p><p><strong>Abstract: </strong>HIFs are transcription factors responsible for sensing low oxygen levels and, in response, inducing the transcription of numerous genes. One of the main processes stimulated by HIFs is the formation of new vessels to increase oxygen supply to the tissue. Formation of the corpus luteum strongly depends on the vasculature, and active angiogenesis occurs during luteinization. In this study, we aimed to analyze the role of HIF in the early formation of corpus luteum and its function, and in female fertility. To this aim, we superovulated mice using equine chorionic gonadotropin and human chorionic gonadotropin (hCG) and administered the HIF inhibitor acriflavine (ACF) to the mice 3 h before hCG. We found a decrease in ovarian HIF1A and VEGFA and in the vascular area in the animals treated with ACF. Moreover, we observed an increase in aberrant structures in the ovaries and in luteal cell apoptosis. Serum progesterone levels were decreased together with ovarian STAR expression. However, the animals treated with ACF during the early formation of the corpus luteum were completely fertile and no alterations were observed when the treated females were mated with fertile males. These results collectively suggest that HIF regulates gonadotropin-induced corpus luteum formation by acting on luteal blood vessel formation, luteal cell survival and progesterone synthesis. However, adequate HIF activity may not be essential to achieve and maintain pregnancy. These findings are significant to better understand the complex mechanisms of corpus luteum formation and identify potential abnormalities to allow better knowledge of ovarian physiology and pathologies in which this factor could be involved.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special series on impact of real-life environmental exposures on reproduction.","authors":"Jodi A Flaws, Vasantha Padmanabhan","doi":"10.1530/REP-24-0454","DOIUrl":"10.1530/REP-24-0454","url":null,"abstract":"","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of PI3K-AKT and MEK-ERK1/2 signaling-driven molecular changes in granulosa cells.","authors":"Vijay Simha Baddela, Marten Michaelis, Xuelian Tao, Dirk Koczan, Julia Brenmoehl, Jens Vanselow","doi":"10.1530/REP-24-0317","DOIUrl":"10.1530/REP-24-0317","url":null,"abstract":"<p><strong>In brief: </strong>PI3K-AKT signaling activates steroidogenesis by inducing estradiol and progesterone production, while MEK-ERK1/2 signaling regulates steroidogenesis by inhibiting estradiol and inducing progesterone production in granulosa cells (GCs). Both pathways are essential for glycolytic and mitochondrial metabolism in these cells.</p><p><strong>Abstract: </strong>The PI3K-AKT and MEK-ERK1/2 signaling pathways are integral to fundamental cellular processes, such as proliferation, viability and differentiation. In GCs, these pathways are activated by follicle-stimulating hormone (FSH) and IGF1 through respective receptors. We investigated the comparative transcriptome changes induced by the AKT and ERK (ERK1/2) pathways using corresponding inhibitors in GCs. GCs isolated from antral follicles showed positive signals for phospho-AKT and phospho-ERK proteins. Treatment of cultured GCs with FSH and IGF1 induced phospho-AKT and phospho-ERK levels. Transcriptome analysis revealed 1436 genes regulated by AKT and 654 genes regulated by the ERK pathway. Among these, 94 genes were commonly downregulated and 11 genes were commonly upregulated in both datasets, while 110 genes were oppositely regulated. Bioinformatics analysis revealed that the inhibition of the PI3K-AKT and MEK-ERK pathways downregulates key reproductive processes and upstream molecules. Notably, AKT inhibition affected FSH, ESRRG and HIF1 pathways, while ERK inhibition impacted CG, FOS, TGFβ, EGR1 and LH pathways. Transcriptome data showed that genes related to estradiol production were inhibited by ERK and induced by the AKT pathway. This was verified by radioimmunoassays, and mRNA and protein analysis of CYP19A1 and STAR genes. In addition, transcriptome data suggested the downregulation of glucose metabolism in GCs. Using validation experiments, we confirm that both pathways are essential for glucose uptake, lactate production and mitochondrial activity in GCs. These data provide a resource for informing future research for analyzing various novel candidate genes regulated by the AKT and ERK pathways in GCs and other cell types.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11774274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP1γ1 is unable to substitute for the mammal-specific PP1γ2 isoform to support male fertility and sperm function.","authors":"Souvik Dey, Wesam Nofal, Cameron Brothag, Mustfa Kabi, Aditi Khamamkar, Neha Choudhari, Srinivasan Vijayaraghavan","doi":"10.1530/REP-24-0256","DOIUrl":"10.1530/REP-24-0256","url":null,"abstract":"<p><strong>In brief: </strong>Protein phosphatase 1 catalytic subunit gamma isoform 2 (PP1γ2) is a unique phosphatase expressed only in mammalian testes and sperm cells. The PP1γ2 isoform is indispensable for sperm motility and fertility and cannot be replaced by the PP1γ1 isoform for these functions.</p><p><strong>Abstract: </strong>The serine-threonine phosphatase has four paralogs - PP1α, PP1β, PP1γ1 and PP1γ2 - encoded by three genes, Ppp1ca, Ppp1cb and Ppp1cc. Protein phosphatase PP1γ2, one of two isoforms of the gene Ppp1cc, is expressed in spermatogenic cells in the testes and sperm, while PP1γ1 is found in somatic cells. The two PP1γ isoforms, formed by alternate splicing that occurs only in mammals, are identical except at their C-termini. Global or testis-specific knockout of Ppp1cc in mice results in male infertility due to disrupted spermiation and mid-to-late spermiogenesis. Transgenic expression of PP1γ2, driven by a testis-specific promoter in differentiating spermatogenic cells, rescues spermatogenesis and fertility in the Ppp1cc-null mice. Why PP1γ2 is essential and present only in mammalian sperm is a mystery. We have generated a knock-in mouse where the Ppp1cc gene is edited to express only PP1γ1. Spermatogenesis was normal in knock-in mice. Testis-expressed PP1γ1 in the knock-in mice and PP1γ2 in the wild-type mice were incorporated in equal amounts into sperm. Sperm bearing PP1γ1 have reduced flagellar beat amplitude and motility, and male mice were severely sub-fertile. Although in the wild-type mice, PP1γ2 is present in both the head and tail, in the knock-in mice, PP1γ1 is absent in sperm heads, leading to an altered intra-sperm protein phosphatase landscape. Phosphoproteomic analysis of sperm proteins suggested a plausible molecular basis for compromised PP1γ1 functions: it identified GSK3α, a known substrate of PP1, to be dysregulated in knock-in sperm. This study provides a preliminary explanation for the isoform-specific requirement of PP1γ2 for male fertility.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNA-novel-216 regulated fatty acid composition and progesterone synthesis in goat granulosa cells by targeting the TPD52.","authors":"Yuhang Jia, Qiao Xu, Peng Wang, Ziyi Liu, Runan Zhang, Kai Liu, Yinghui Ling, Yufang Liu, Mingxing Chu","doi":"10.1530/REP-24-0231","DOIUrl":"10.1530/REP-24-0231","url":null,"abstract":"<p><strong>In brief: </strong>Steroid hormone secretion by granulosa cells in the ovary is regulated by lipid synthesis and metabolism. This study found that miR-novel-216 affects granulosa cell proliferation and free fatty acid content by regulating TPD52 expression, thereby increasing the reproductive hormone secretion and promoting polytocous trait in goats.</p><p><strong>Abstract: </strong>Granulosa cells in the ovaries of livestock are crucial for secreting steroid hormones that regulate follicular development, with lipid synthesis and metabolism playing key roles in this process. The molecular mechanisms behind steroid hormone secretion regulated by fatty acid metabolism in goat granulosa cells have been unclear. Our previous transcriptome analysis of Yunshang black goat ovaries revealed that miR-novel-216, which had lower expression in high-fertility goats, might regulate granulosa cell function. We further investigated the role of miR-novel-216 by isolating and culturing goat granulosa cells in vitro and found that it inhibits cell proliferation, lipid accumulation and progesterone synthesis in goat granulosa cells. The qTar and miRanda analyses predicted TPD52 as a target of miR-novel-216, confirmed by dual luciferase and transfection assays. Previous studies have shown that progesterone synthesis in granulosa cells is closely related to free fatty acid composition. We investigated the effect of in vitro construction of TPD52 overexpression and interference plasmids on the free fatty acid content of goat granulosa cells using mass spectrometry sequencing. The results showed that the overexpression of TPD52 in goat granulosa cells significantly increased the free fatty acid content and promoted granulosa cell proliferation, lipid accumulation and progesterone synthesis, whereas the opposite was true for inhibition of TPD52. It was shown that miR-novel-216 affects granulosa cell proliferation and free fatty acid levels by regulating the expression of TPD52, which increases reproductive hormone secretion and promotes polytocous trait in goats. This provides a foundation for developing breeding strategies to improve goat fertility.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic syndrome impairs endometrial functioning and early pregnancy: an in vivo study.","authors":"Noelia Carnovale, Candela Velazquez, Sofía Del Valle, Julieta Simone, Luis Francisco Méndez García, Analy Fritzler, Jorge Palazzi, Inés Stella, Mariela Bilotas, Gabriela Meresman","doi":"10.1530/REP-24-0321","DOIUrl":"10.1530/REP-24-0321","url":null,"abstract":"<p><strong>In brief: </strong>This study demonstrated that metabolic syndrome (MetS) in mice disrupts the estrous cycle, increases progesterone levels and alters the endometrial structure, resulting in impaired reproductive success. Treatment with metformin effectively reversed these effects, restoring hormonal balance and normal endometrial architecture and improving reproductive outcomes.</p><p><strong>Abstract: </strong>MetS is increasingly associated with impaired reproductive health. This study aimed to assess the endometrial characteristics and reproductive outcomes of a female MetS mouse model and evaluate metformin's therapeutic effects. Twenty-one-day-old female C57BL/6 mice were randomly divided into a high-fat (HF) diet group (n = 50) and a control group (n = 30) that received standard chow. After 11 weeks, a subset of HF mice (n = 25) was given oral metformin at 300 mg/kg/day, while the other ones continued on HF diet. After 15 weeks, mice were either sacrificed during estrus or mated and euthanized on day 7.5 of pregnancy (n = 15 per group). The estrous cycle, progesterone and estradiol levels, uterine morphology, endometrial cell proliferation, reproductive performance and metformin's treatment effects were assessed. Mice on the HF diet developed MetS, which was characterized by moderate glycemic dysregulation, increased cholesterol, insulin resistance and central obesity. Experimental MetS caused estrous cycle disruptions and increased serum progesterone levels, which were normalized by metformin. MetS also affected endometrial histology, producing hyperplasia and altering cell proliferation, while metformin restored the normal endometrial architecture by inhibiting cell proliferation. In addition, MetS impaired the reproductive success by delaying coitus and reducing the ratio of implantation sites to corpora lutea, both of which were rectified by metformin. In conclusion, MetS adversely affects reproductive function, but metformin offers improvement. Our findings highlight the need for further research on the impact of MetS on reproduction and the exploration of treatments to enhance reproductive health in women with MetS.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal proteoforms from bulls with contrasting semen freezability: a story deciphered by top-down mass spectrometry.","authors":"Arabela Guedes de Azevedo Viana, Fabio Pereira Gomes, Abdullah Kaya, Jolene Diedrich, Einko Topper, Dario Di Silvestre, Mariana Machado-Neves, Erdogan Memili, Arlindo Alencar Moura, John R Yates","doi":"10.1530/REP-24-0051","DOIUrl":"10.1530/REP-24-0051","url":null,"abstract":"<p><strong>In brief: </strong>Bovine sperm and seminal plasma (SP) proteoform atlas was characterized using top-down proteomics. Specific post-translational modifications and protein truncations correlated with semen freezability, with potential links to sperm functional processes.</p><p><strong>Abstract: </strong>Top-down proteomics was employed to construct a proteoform atlas of sperm and SP from bulls with low semen freezability (LF) and high semen freezability (HF). Sperm and seminal proteins were fractionated by tandem size exclusion chromatography (<30 kDa) and analyzed by reversed-phase liquid chromatography-tandem mass spectrometry. This approach enabled identification of 299 SP (from 46 families) and 267 sperm proteoforms (from 139 families). Seventy proteoforms belonging to beta-defensin 10, c-type natriuretic peptide (NPPC), caltrin, seminal ribonuclease, osteopontin and binder of sperm protein (BSP) 3 families were unique to HF bulls' SP. LF seminal proteins had 77 unique proteoforms, including caltrin, NPPC, osteopontin, BSP3, serpin family A member 5 and β-NGF families. Proteoform families of SP in HF and LF bulls were related to Ca2+ uptake, capacitation, acrosome reaction, sperm protection, fertilization and proteolytic processes. Thirty-three proteoforms of NPPC, caltrin and cylicin-2 families were upregulated in HF sperm. Twenty-two proteoforms of caltrin, cylicin-2, adenosine triphosphate (ATP) synthases and malate dehydrogenase families were among those upregulated in LF sperm. Truncated and acetylated histone H2A and non-truncated and acetylated c-Myc-binding protein were prevalent in LF sperm. Cylicin-2 proteoforms were observed in HF and LF sperm, and truncated glyceraldehyde-3-phosphate dehydrogenases were observed only in HF sperm. In silico analyses indicated the enrichment of mitochondrial metabolic pathways in HF sperm, including fatty acid metabolism and TCA cycle. Our study provides an unprecedented description of the bovine SP and sperm proteoforms. Post-translational processing appears to define the bioproperties of semen proteins and their associations with sperm cryoresistance.</p>","PeriodicalId":21127,"journal":{"name":"Reproduction","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}