Research Journal of Biotechnology最新文献

{"title":"Salicylic acid improving physiological and phytochemical changes in Stevia rebaudiana under salt stress and estimating steviol glycoside by LC-MS/MS","authors":"Zahabiya Dhankot, Gaurav Sanghvi","doi":"10.25303/1901rjbt1100121","DOIUrl":"https://doi.org/10.25303/1901rjbt1100121","url":null,"abstract":"Almost every area of plant development is negatively impacted by stress. Salicylic acid (SA), when supplied exogenously, has been demonstrated to be a regulatory hormone that may mitigate the harmful effects of salt stress. Diabetics, hypertensive and health-conscious people substitute Stevia rebaudiana for sugar. Salinity is the global problem directly affecting the quality and quantity of stevia. Each group received 100 mM NaCl treatment for seven days followed by 0.1 mM, 0.2 mM and 0.05 mM SA treatment. Salt stress reduces the amount of chlorophyll (P<0.001) and the antioxidant capacity (P<0.001) which is restored by SA treatment. Salt stress increased proline (P<0.001), MDA (P<0.001) and other antioxidant enzymes (P<0.001), with the exception of catalase (P<0.05). Furthermore, salt stress has a negative impact on stevioside production which SA assisted in improving. Salt stress hampered the biology and phytochemistry of Stevia. SA restored antioxidants, chlorophyll, proline and phytochemicals. SA also helped to improve the steviol production in salt stress which is significantly lower in normal plants. Method developed to estimate steviol level can also be helpful to different industries involved in stevia products.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"174 ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138985254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of feed on the yield of Litopenaeus vannamei and Macrobrachium rosenbergii prawn culture from West Godavari district, Andhra Pradesh, India","authors":"B. Sravanthi, Ch. Lalitha, V. Lakshmi","doi":"10.25303/1901rjbt1050109","DOIUrl":"https://doi.org/10.25303/1901rjbt1050109","url":null,"abstract":"Aquaculture, a vital sector addressing global food demand, is characterized by its extensive water requirements. Categorized into mariculture, freshwater aquaculture, and brackish water aquaculture, the sector plays a crucial role in food production. This study focuses on prawn cultivation, a significant aspect of aquaculture, emphasizing the influence of feed quality and hydrographic parameters on yield and ecological sustainability. Conducted in Vissakoderu village (Litopenaeus vannamei) and Eduru village (Macrobrachium rosenbergii), the research employed commercial feed, optimizing physicochemical parameters such as temperature, pH, and salinity. Probiotics were integrated into the feed to enhance prawn growth. Results indicated optimal levels of moisture, lipids, protein, fiber, carbohydrates, minerals, and vitamins in the feed. The progressive weight gain of prawns, reaching 30.00 kg for 1000 prawns, was correlated with an increased feeding frequency. Scientifically monitored hydrographic conditions and feed quality contributed to a remarkable average yield of 29 tons for Litopenaeus vannamei and 63.33 tons for Macrobrachium rosenbergii over three years. This underscores the importance of precision in aquaculture practices for both yield enhancement and ecological preservation. The study provides valuable insights into the sensitivity of prawn cultivation, emphasizing the need for meticulous care in managing hydrographical, feed, and disease parameters.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"297 ","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138985042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation, Phytochemical screening and Antimicrobial analysis of caterpillar mushroom Cordyceps militaris and fruiting body","authors":"Aanchal Gupta, Chandra Kant Sharma, Monika Sharma","doi":"10.25303/1901rjbt022032","DOIUrl":"https://doi.org/10.25303/1901rjbt022032","url":null,"abstract":"The fungus Cordyceps militaris comes under the family cordyciptiaceae and class ascomycetes. In ancient time it was used in Chinese and Tibetan medicines for lungs and kidney diseases but with the change in era, more researches were conducted on it. This study was designed for the cultivation of Cordyceps militaris (C.M.) mushroom on brown rice media as the measure component and evaluation of its quantitative, qualitative and free radical scavenging activity of the methanolic extract. To know the bioactive compounds, preliminary phytochemical analysis was done which states that methanolic extract of Cordyceps contains protein, phenol, alkaloids, tannin, flavonoids, coumarins, polysaccharides and carbohydrates. The quantitative analysis of methanolic extract was quantified using determination of total phenol and total flavonoids using Folin-Ciocalteu reagent and Aluminium calorimetric assay method respectively. Phyto- compounds were depicted through Fourier transform infrared spectroscopy (FTIR) and Gas chromatography- mass spectroscopy (GC-MS) while the free radical scavenging activity was determined using the 1,1-diphenyl-2-pycryl-hydrazyl (DPPH) assay, hydrogen peroxide assay, ferric reducing antioxidant power (FRAP) assay and reducing power assay. Highest phenolic and flavonoid contents were depicted in CM100%meth while CM100%meth. showed highest free radical scavenging activity. The assessment of extract for antimicrobial activity was done with well diffusion method against Gram positive and Gram negative bacteria revealing that aqueous and methanolic extract are more effective against Bacillus subtilis and Escherichia coli.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"15 4","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138984721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico evaluation of isoflavones for its galactagogue activity in promoting lactation","authors":"Ravichandran Nikkila Devi, Vasudevan Dhisha, Balasubramanian Suresh, Sundarapandian Mahalakshmi","doi":"10.25303/1811rjbt01060114","DOIUrl":"https://doi.org/10.25303/1811rjbt01060114","url":null,"abstract":"Breastfeeding is crucial for the health of both mothers and infants, providing both short-term and long-term benefits. Breast milk contains all the necessary nutrients for both the mother and child. Unfortunately, many women are experiencing a low milk supply, resulting in infants not receiving sufficient immune support. To increase milk production, there are various herbal and pharmaceutical galactagogues available. Plants in the Caryophyllaceae family contain phytoestrogen, a compound that helps stimulate milk production. Isoflavone is one type of phytoestrogens. Isoflavones have many properties like bactericidal, fungicidal and anti-ringworm and are used to treat breast and prostate cancer and bone-related diseases. Spergula arvensis L., a member of the Caryophyllaceae family, is rich in isoflavones. Nine specific compounds were chosen due to their connection to mammary gland function. A preliminary in silico study was conducted to examine the interaction between isoflavones and prolactin receptors through molecular docking. The compound quercetin, an isoflavone, showed a better binding affinity to the receptor.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Cloning and Expression Analysis of CONSTANS-LIKE 2 in Mucuna bracteata in Different Tissues and at Low Temperature","authors":"Shariff Misfahulhairah, Namasivayam Parameswari, Nur Fatihah Mohd-Yusoff","doi":"10.25303/1811rjbt01010","DOIUrl":"https://doi.org/10.25303/1811rjbt01010","url":null,"abstract":"Mucuna bracteata is a leguminous cover crop that is planted in Malaysian oil palm and rubber plantations. This legume can grow well locally but is unable to produce flowers. Non-flowering M. bracteata plants presumably do not perceive the flowering signals to initiate the reproductive phase due to environmental differences. This study is on cloning a putative flowering gene, CONSTANS-LIKE 2 (COL2), from leaves of M. bracteata. To understand the molecular regulation of this gene in locally grown M. bracteata, we analysed its expression profile in different tissues of M. bracteata seedlings and response to low temperature using real-time polymerase chain reaction. The MbCOL2 gene was identified to be expressed in all selected tissues; leaf, terminal bud and stem of 2 months, 6 months and 12 months-old seedlings in different patterns. The MbCOL2 expression was the highest in the leaf tissue. Meanwhile, the expression of MbCOL2 was increased due to the low temperature. Physicochemical properties of MbCOL2 and its orthologues were also defined to analyse their protein functional properties. Outputs from this study can assist to better understand the molecular regulation of growth and development in M. bracteata.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135766471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological application prospects of latex proteases from Apocynaceae family","authors":"Vasuki Aluru, Mrinalini Menon, Omana Bindhu Sukumaran","doi":"10.25303/1811rjbt2330240","DOIUrl":"https://doi.org/10.25303/1811rjbt2330240","url":null,"abstract":"Plant latex proteases from different species find applications in diverse industries ranging from food to medicine. Family Apocynaceae consists of species producing large quantities of milky latex. Latex is known to be a rich source of proteolytic enzymes or proteases. Proteases, mainly of the cysteine and occasionally serine type, have been characterized from the latices of different species of Apocynaceae. Many of these proteases have also demonstrated stability under a wide range of parameters including temperature, pH and presence of metal ions. The current review is an attempt to elucidate on latex proteases belonging to Apocynaceae family, highlighting those properties that offer potential for their large-scale applications in different industrial sectors.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"234 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135766477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of express sequence tags from Arbuscular Mycorrhizal fungi (AMF) express during the pre-symbiotic stage of the fungus","authors":"Sanjeev Kumar, Alok Adholeya","doi":"10.25303/1811rjbt089095","DOIUrl":"https://doi.org/10.25303/1811rjbt089095","url":null,"abstract":"Arbuscular Mycorrhizal Fungi (AMF) are symbiotically associated with the roots of higher plants which enhance the overall nutritional status of plants. It is known that the overall growth and development of plants not only depend upon the performance of specific genotypes but their association is also affected by the various types of microbes. Therefore, the major aim of the study was the isolation and characterization of gene expression during the presymbiotic stage of AMF. In the present study, total RNA was extracted from spores of Rhizophagus irregularis grown under root organ culture (ROC). cDNA library was prepared and expressed sequence tag (EST) analysis was performed. Approximately 150 random clones were selected for EST sequencing analysis. Out of the 150 clones obtained, 1.5% carried inserts of the rRNA gene cluster. After excision, inserts from 72 randomly selected clones were sequenced. Database searches revealed that 74% of the clones had similarities to already known sequences. Putative genes for Ca2+-regulated symbiotic genes, cell cycle regulatory factors and precursors for lipid biosynthesis genes were discovered. A full-length mating gene was discovered using rapid amplification of cDNA ends (RACE). Studies revealed the presence of a putative mating type gene in mycorrhizal species that are evident in the sexual origin of AMF.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135766479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of aqueous and ethanolic seed extract of Asparagus racemosus Linn on neurobehavioral pattern of acrylamide induced experimental Zebra fish","authors":"Anubhav Dubey, Niladry S. Ghosh, Ranjit Singh","doi":"10.25303/1811rjbt081088","DOIUrl":"https://doi.org/10.25303/1811rjbt081088","url":null,"abstract":"A neuropsychological assessment is a test that assesses how well a person's brain functions. Literacy, language use, attention, learning, processing speed, reasoning, memory, problem-solving, personality and other talents are all assessed. The purpose of this study is to assess the effects of aqueous and ethanolic seed extracts of Asparagus racemosus Linn on the neurobehavioral pattern of acrylamide-induced experimental Zebra fish. In the novel tank test, the number of top entries, top remain time, top entry latency and total distance traveled were all greater in the Asparagus racemosus Linn seed extract group than in the acrylamide group, while the number of bottom entries and bottom duration of stay were lower. In the light-dark test, group II Zebrafish treated with 0.75 mM acrylamide showed anxiety by spending less time in the light zone. In our study, Zebrafish pre-treated with aqueous and ethanolic seed extracts of different concentrations of Asparagus racemosus Linn significantly reversed. In the open field test, the Asparagus racemosus Linn seed extract at different doses significantly improved outer zone (s) and inner zone (S) times compared to the acrylamide control group. In the Y-maze test, the Asparagus racemosus Linn seed extract at different dosages exhibited a dose-influenced significant improvement in the number of entry points to new arms and the total distance walked in the maze in comparison to the acrylamide control group. When compared to the acrylamide control, the Asparagus racemosus Linn seeds extract at various doses demonstrated a significant rise in the amount of time spent in the new arm.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of Marek's disease virus revealing the predominance of high-virulence MDV in Vietnam","authors":"Thi Nguyen Khue, Thi Thu Hien Nguyen, Thanh Huyen Le Thi, Thi Do Roan, Thi Thanh Doan Huong","doi":"10.25303/1811rjbt070080","DOIUrl":"https://doi.org/10.25303/1811rjbt070080","url":null,"abstract":"Marek's disease (MD) is a potentially fatal viral disease caused by the Gallid alphaherpesvirus 2 (GaHV-2). The outbreaks are common in both unprotected and vaccinated flocks. The carcinogenic serotype MDV-1 was found using a PCR method in five northern provinces and two central provinces of Vietnam from 2019 to 2022. The Meq gene from 13 MDV-1 strains was sequenced in its entirety, containing 1020 nucleotides encoding 339 amino acids. The examination of these genomes revealed that all strains discovered in Vietnam correspond to the pathotypes known as highly virulent (vv). This classification was based on the presence of low proline concentration (from 20.59-21.18%) in the Meq which consisted of three PPPP motifs and three interrupted motifs. The interrupted motifs are characterized by mutations at the second position of the proline-rich region where the original PPPP sequence is altered to PPPP&gt;P (Q / A / R) PP. Furthermore, the Vietnamese GaHV-2 strains in this study had the most similarity to high-virulence Chinese strains but had the lowest similarity to the currently used CVI988 vaccination. It is worth noting that no strains from the moderate pathogenicity (mv) or very virulence plus (vv+) groups were found among the Vietnamese isolates. The findings shed light on previously unknown molecular features of Marek's disease virus strains in Vietnam's northern provinces. The study's findings add to a better understanding of MDV in Vietnam, filling an important information gap. This knowledge can help guide efforts to reduce the burden of Marek's disease on the chicken business, improve disease prevention and control strategies and select the best vaccine for chickens in the field.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, Purification, Characterization and Anti biofilm activity of natural antibiotic by Actinokineospora cibodasensis from marine water","authors":"K.S. Sreelakshmi, R. Usha","doi":"10.25303/1811rjbt01420153","DOIUrl":"https://doi.org/10.25303/1811rjbt01420153","url":null,"abstract":"Health sector faces a distress globally because of the scarcity of newer antibiotics against drug resistant pathogens. Innovations in developing new antibiotics is the solution to combat the drug resistant pathogens. This alarming situation requires advanced, potential and effective antibiotics. Secondary metabolite products from microbes remain as the main origin for many of the antibiotics in the market today and are also found to be promising till date. Out of the microbes, the major share of antibiotic production is reported by Actinomycetes, which are Gram positive, anaerobic, filamentous bacteria. They are currently being exploited through new approaches to improve the efficiency of commercially valuable antibiotics. Since natural environment stands a potential source of innovative and novel antimicrobials, this investigation was performed to test the potential of selected marine actinomycetes. Of the screened ones, Actinokineospora is potential enough to cease the growth of biofilm formation. This strain was identified by molecular characterization as Actinokineospora cibodasensis. It was selected to extract secondary metabolites by submerged fermentation and made to undergo further purification and structural analyses for the search of bioactive components. Presence of streptomycin like compound by Gas Chromatography Mass Spectrometry led to a breakthrough in the study which had been confined by High Performance Thin Layer Chromatography and Fourier Transform Infra-Red Spectroscopy techniques by comparing with standards. Gene expression studies showed that the selected strain can down regulate the genes in the formation of biofilm by the test organism Pseudomonas aeruginosa. The bioactive compound from Actinokineospora is an ecofriendly product along with a biofilm mitigating activity on medical devices. Rare actinomycetal isolate was able to produce the antibiotic which could apply on medical device to reduce the infections during the treatment. This antibiotic coating on life saving devices will provide the ultimate protection for patients.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}