Research Journal of Biotechnology最新文献_第2页

Phytochemicals and Phytotherapeutic Studies on Different Parts of Aegle marmelos: A Critical Overview 关于 Aegle marmelos 不同部位的植物化学物质和植物治疗研究：重要概述

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-12-05 DOI: 10.25303/1901rjbt1260148

Y. Murti, K. K. Agrawal

引用次数: 0

Detrimental Effects of Lithium on in vitro Seedlings of Pea (Vigna radiata) 锂对豌豆（Vigna radiata）离体幼苗的有害影响

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-12-05 DOI: 10.25303/1901rjbt087090

Davis Dilsha, S. Narasimhan

{"title":"Detrimental Effects of Lithium on in vitro Seedlings of Pea (Vigna radiata)","authors":"Davis Dilsha, S. Narasimhan","doi":"10.25303/1901rjbt087090","DOIUrl":"https://doi.org/10.25303/1901rjbt087090","url":null,"abstract":"The global demand for lithium-ion batteries has raised concerns about environmental consequences stemming from lithium leaching. In this study, we investigated the effects of lithium, sourced from Li2SO4, on in vitro seed germination and seedling growth of green moong (Vigna radiata). Our experiments used woody plant medium supplemented with 1 mg/l naphthalene acetic acid (NAA) and 2 mg/l benzyl adenine (BA). We observed that in vitro seed germination remained unaffected by lithium concentration. However, at concentrations of 3-5 mg/l of lithium, significant changes were observed in stem parameters including growth reduction, shoot tip damage and stem discolouration. Root growth was inhibited at lithium concentrations of 4 and 5 mg/l. Additionally, we noted leaf abscission, dark colouration and reduced leaf area in a dose-dependent manner. Necrotic lesions were not observed in the seedlings. Chlorophyll accumulation, phenolic content and peroxidase (POD) activity showed enhancement with increasing lithium concentration. Our data analysis strongly suggests dose-dependent toxicity in young seedlings under in vitro conditions. These findings shed light on the potential ecological implications of lithium leaching from retired batteries.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138984901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of extraction of total flavonoid from leafs of Helicteres hirsuta Lour. based on bath ultrasound-assisted and testing some biological activities 基于水浴超声波辅助的 Helicteres hirsuta Lour.叶片总黄酮提取优化及生物活性测试

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-12-05 DOI: 10.25303/1901rjbt071078

Dang Thanh Long, Van Thi My Le, Hoàng Thị Ngọc Hân, Tong Thi Hue, Hoang Thi Kim Hong

{"title":"Optimization of extraction of total flavonoid from leafs of Helicteres hirsuta Lour. based on bath ultrasound-assisted and testing some biological activities","authors":"Dang Thanh Long, Van Thi My Le, Hoàng Thị Ngọc Hân, Tong Thi Hue, Hoang Thi Kim Hong","doi":"10.25303/1901rjbt071078","DOIUrl":"https://doi.org/10.25303/1901rjbt071078","url":null,"abstract":"Helicteres hirsuta Lour. contains highly bioactive compounds that have therapeutic effects on many diseases, hence are used to make medicines from most parts of the plant. We optimize using the RSM surface response method based on Design Expert 11 software (Stat-Ease Inc, Minneapolis, USA) and analyze LC-MS/MS method and some other biochemical methods of methanol extraction from leaves of H. hirsuta L. collection Thua Thien Hue, Vietnam. The results identified the ideal parameters for extracting the greatest total flavonoids from leaves of H. hirsuta L. as follows: Times (30 minutes); methanol solvent concentration (52.2407%); ultrasonic frequency (12.4253); and material/solvent ratio (1:19.9145 (w/v)). The total flavonoid value obtained was 7.78425 (mg Catechin/g extract). The actual experiment obtained an actual value of 7.538 (mg Catechin/g extract). The results obtained 22 compounds of a flavonoid nature (61.111%) appearing in the purified methanol fractional extract. These compounds can inhibit DPPH free radicals at 50% with an IC50 value of 521.498 g/mL and they also have inhibitory activity against the growth of cancer cell lines under study with IC50 values ranging from 104.02 to 188.48 g/mL. The best cytotoxic effect of methanol extract from leaves of H. hirsute L. on human leukemia cell line (HL-60) with IC50 was 104.02 μg/mL. The results obtained indicate that leaves of H. hirsute L. have prospective uses as therapeutic herbs in antioxidant and cancer cell-inhibiting properties.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138984942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salicylic acid improving physiological and phytochemical changes in Stevia rebaudiana under salt stress and estimating steviol glycoside by LC-MS/MS 水杨酸改善盐胁迫下甜叶菊的生理和植物化学变化，并通过 LC-MS/MS 评估甜菊醇苷的含量

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-12-05 DOI: 10.25303/1901rjbt1100121

Zahabiya Dhankot, Gaurav Sanghvi

{"title":"Salicylic acid improving physiological and phytochemical changes in Stevia rebaudiana under salt stress and estimating steviol glycoside by LC-MS/MS","authors":"Zahabiya Dhankot, Gaurav Sanghvi","doi":"10.25303/1901rjbt1100121","DOIUrl":"https://doi.org/10.25303/1901rjbt1100121","url":null,"abstract":"Almost every area of plant development is negatively impacted by stress. Salicylic acid (SA), when supplied exogenously, has been demonstrated to be a regulatory hormone that may mitigate the harmful effects of salt stress. Diabetics, hypertensive and health-conscious people substitute Stevia rebaudiana for sugar. Salinity is the global problem directly affecting the quality and quantity of stevia. Each group received 100 mM NaCl treatment for seven days followed by 0.1 mM, 0.2 mM and 0.05 mM SA treatment. Salt stress reduces the amount of chlorophyll (P<0.001) and the antioxidant capacity (P<0.001) which is restored by SA treatment. Salt stress increased proline (P<0.001), MDA (P<0.001) and other antioxidant enzymes (P<0.001), with the exception of catalase (P<0.05). Furthermore, salt stress has a negative impact on stevioside production which SA assisted in improving. Salt stress hampered the biology and phytochemistry of Stevia. SA restored antioxidants, chlorophyll, proline and phytochemicals. SA also helped to improve the steviol production in salt stress which is significantly lower in normal plants. Method developed to estimate steviol level can also be helpful to different industries involved in stevia products.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138985254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation, Phytochemical screening and Antimicrobial analysis of caterpillar mushroom Cordyceps militaris and fruiting body 虫草菌及子实体的栽培、植物化学筛选和抗菌分析

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-12-05 DOI: 10.25303/1901rjbt022032

Aanchal Gupta, Chandra Kant Sharma, Monika Sharma

{"title":"Cultivation, Phytochemical screening and Antimicrobial analysis of caterpillar mushroom Cordyceps militaris and fruiting body","authors":"Aanchal Gupta, Chandra Kant Sharma, Monika Sharma","doi":"10.25303/1901rjbt022032","DOIUrl":"https://doi.org/10.25303/1901rjbt022032","url":null,"abstract":"The fungus Cordyceps militaris comes under the family cordyciptiaceae and class ascomycetes. In ancient time it was used in Chinese and Tibetan medicines for lungs and kidney diseases but with the change in era, more researches were conducted on it. This study was designed for the cultivation of Cordyceps militaris (C.M.) mushroom on brown rice media as the measure component and evaluation of its quantitative, qualitative and free radical scavenging activity of the methanolic extract. To know the bioactive compounds, preliminary phytochemical analysis was done which states that methanolic extract of Cordyceps contains protein, phenol, alkaloids, tannin, flavonoids, coumarins, polysaccharides and carbohydrates. The quantitative analysis of methanolic extract was quantified using determination of total phenol and total flavonoids using Folin-Ciocalteu reagent and Aluminium calorimetric assay method respectively. Phyto- compounds were depicted through Fourier transform infrared spectroscopy (FTIR) and Gas chromatography- mass spectroscopy (GC-MS) while the free radical scavenging activity was determined using the 1,1-diphenyl-2-pycryl-hydrazyl (DPPH) assay, hydrogen peroxide assay, ferric reducing antioxidant power (FRAP) assay and reducing power assay. Highest phenolic and flavonoid contents were depicted in CM100%meth while CM100%meth. showed highest free radical scavenging activity. The assessment of extract for antimicrobial activity was done with well diffusion method against Gram positive and Gram negative bacteria revealing that aqueous and methanolic extract are more effective against Bacillus subtilis and Escherichia coli.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138984721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of feed on the yield of Litopenaeus vannamei and Macrobrachium rosenbergii prawn culture from West Godavari district, Andhra Pradesh, India 饲料对印度安得拉邦西戈达瓦里地区养殖的凡纳滨对虾和罗氏沼虾产量的影响

IF 0.2

Research Journal of Biotechnology Pub Date : 2023-12-05 DOI: 10.25303/1901rjbt1050109

B. Sravanthi, Ch. Lalitha, V. Lakshmi

{"title":"Impact of feed on the yield of Litopenaeus vannamei and Macrobrachium rosenbergii prawn culture from West Godavari district, Andhra Pradesh, India","authors":"B. Sravanthi, Ch. Lalitha, V. Lakshmi","doi":"10.25303/1901rjbt1050109","DOIUrl":"https://doi.org/10.25303/1901rjbt1050109","url":null,"abstract":"Aquaculture, a vital sector addressing global food demand, is characterized by its extensive water requirements. Categorized into mariculture, freshwater aquaculture, and brackish water aquaculture, the sector plays a crucial role in food production. This study focuses on prawn cultivation, a significant aspect of aquaculture, emphasizing the influence of feed quality and hydrographic parameters on yield and ecological sustainability. Conducted in Vissakoderu village (Litopenaeus vannamei) and Eduru village (Macrobrachium rosenbergii), the research employed commercial feed, optimizing physicochemical parameters such as temperature, pH, and salinity. Probiotics were integrated into the feed to enhance prawn growth. Results indicated optimal levels of moisture, lipids, protein, fiber, carbohydrates, minerals, and vitamins in the feed. The progressive weight gain of prawns, reaching 30.00 kg for 1000 prawns, was correlated with an increased feeding frequency. Scientifically monitored hydrographic conditions and feed quality contributed to a remarkable average yield of 29 tons for Litopenaeus vannamei and 63.33 tons for Macrobrachium rosenbergii over three years. This underscores the importance of precision in aquaculture practices for both yield enhancement and ecological preservation. The study provides valuable insights into the sensitivity of prawn cultivation, emphasizing the need for meticulous care in managing hydrographical, feed, and disease parameters.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138985042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico evaluation of isoflavones for its galactagogue activity in promoting lactation 异黄酮促乳促乳活性的计算机评价

Research Journal of Biotechnology Pub Date : 2023-10-15 DOI: 10.25303/1811rjbt01060114

Ravichandran Nikkila Devi, Vasudevan Dhisha, Balasubramanian Suresh, Sundarapandian Mahalakshmi

{"title":"In silico evaluation of isoflavones for its galactagogue activity in promoting lactation","authors":"Ravichandran Nikkila Devi, Vasudevan Dhisha, Balasubramanian Suresh, Sundarapandian Mahalakshmi","doi":"10.25303/1811rjbt01060114","DOIUrl":"https://doi.org/10.25303/1811rjbt01060114","url":null,"abstract":"Breastfeeding is crucial for the health of both mothers and infants, providing both short-term and long-term benefits. Breast milk contains all the necessary nutrients for both the mother and child. Unfortunately, many women are experiencing a low milk supply, resulting in infants not receiving sufficient immune support. To increase milk production, there are various herbal and pharmaceutical galactagogues available. Plants in the Caryophyllaceae family contain phytoestrogen, a compound that helps stimulate milk production. Isoflavone is one type of phytoestrogens. Isoflavones have many properties like bactericidal, fungicidal and anti-ringworm and are used to treat breast and prostate cancer and bone-related diseases. Spergula arvensis L., a member of the Caryophyllaceae family, is rich in isoflavones. Nine specific compounds were chosen due to their connection to mammary gland function. A preliminary in silico study was conducted to examine the interaction between isoflavones and prolactin receptors through molecular docking. The compound quercetin, an isoflavone, showed a better binding affinity to the receptor.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Cloning and Expression Analysis of CONSTANS-LIKE 2 in Mucuna bracteata in Different Tissues and at Low Temperature 苞片粘液中CONSTANS-LIKE 2在不同组织和低温下的分子克隆及表达分析

Research Journal of Biotechnology Pub Date : 2023-10-15 DOI: 10.25303/1811rjbt01010

Shariff Misfahulhairah, Namasivayam Parameswari, Nur Fatihah Mohd-Yusoff

{"title":"Molecular Cloning and Expression Analysis of CONSTANS-LIKE 2 in Mucuna bracteata in Different Tissues and at Low Temperature","authors":"Shariff Misfahulhairah, Namasivayam Parameswari, Nur Fatihah Mohd-Yusoff","doi":"10.25303/1811rjbt01010","DOIUrl":"https://doi.org/10.25303/1811rjbt01010","url":null,"abstract":"Mucuna bracteata is a leguminous cover crop that is planted in Malaysian oil palm and rubber plantations. This legume can grow well locally but is unable to produce flowers. Non-flowering M. bracteata plants presumably do not perceive the flowering signals to initiate the reproductive phase due to environmental differences. This study is on cloning a putative flowering gene, CONSTANS-LIKE 2 (COL2), from leaves of M. bracteata. To understand the molecular regulation of this gene in locally grown M. bracteata, we analysed its expression profile in different tissues of M. bracteata seedlings and response to low temperature using real-time polymerase chain reaction. The MbCOL2 gene was identified to be expressed in all selected tissues; leaf, terminal bud and stem of 2 months, 6 months and 12 months-old seedlings in different patterns. The MbCOL2 expression was the highest in the leaf tissue. Meanwhile, the expression of MbCOL2 was increased due to the low temperature. Physicochemical properties of MbCOL2 and its orthologues were also defined to analyse their protein functional properties. Outputs from this study can assist to better understand the molecular regulation of growth and development in M. bracteata.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135766471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological application prospects of latex proteases from Apocynaceae family 夹竹桃科乳胶蛋白酶的生物技术应用前景

Research Journal of Biotechnology Pub Date : 2023-10-15 DOI: 10.25303/1811rjbt2330240

Vasuki Aluru, Mrinalini Menon, Omana Bindhu Sukumaran

引用次数: 0

Analysis of express sequence tags from Arbuscular Mycorrhizal fungi (AMF) express during the pre-symbiotic stage of the fungus 丛枝菌根真菌(AMF)在真菌共生前阶段表达序列标签的分析

Research Journal of Biotechnology Pub Date : 2023-10-15 DOI: 10.25303/1811rjbt089095

Sanjeev Kumar, Alok Adholeya

{"title":"Analysis of express sequence tags from Arbuscular Mycorrhizal fungi (AMF) express during the pre-symbiotic stage of the fungus","authors":"Sanjeev Kumar, Alok Adholeya","doi":"10.25303/1811rjbt089095","DOIUrl":"https://doi.org/10.25303/1811rjbt089095","url":null,"abstract":"Arbuscular Mycorrhizal Fungi (AMF) are symbiotically associated with the roots of higher plants which enhance the overall nutritional status of plants. It is known that the overall growth and development of plants not only depend upon the performance of specific genotypes but their association is also affected by the various types of microbes. Therefore, the major aim of the study was the isolation and characterization of gene expression during the presymbiotic stage of AMF. In the present study, total RNA was extracted from spores of Rhizophagus irregularis grown under root organ culture (ROC). cDNA library was prepared and expressed sequence tag (EST) analysis was performed. Approximately 150 random clones were selected for EST sequencing analysis. Out of the 150 clones obtained, 1.5% carried inserts of the rRNA gene cluster. After excision, inserts from 72 randomly selected clones were sequenced. Database searches revealed that 74% of the clones had similarities to already known sequences. Putative genes for Ca2+-regulated symbiotic genes, cell cycle regulatory factors and precursors for lipid biosynthesis genes were discovered. A full-length mating gene was discovered using rapid amplification of cDNA ends (RACE). Studies revealed the presence of a putative mating type gene in mycorrhizal species that are evident in the sexual origin of AMF.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135766479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0