Recent patents on biotechnology最新文献

{"title":"Synthesis and Biological Properties of Formulated Skin Serum Containing Coelomic Fluid of Earthworm <i>Eisenia fetida/andrei</i>.","authors":"Parisa Imeni, Mehdi Zarabi, Pegah Shakib, Ashkan Dirbaziyan","doi":"10.2174/0118722083342120250426085714","DOIUrl":"10.2174/0118722083342120250426085714","url":null,"abstract":"<p><strong>Background and objectives: </strong>In this study, the coelomic fluid of Eisenia fetida/ andrei species was used for the first time to prepare an anti-aging serum, and its antioxidant and antibacterial properties were investigated. In addition, its cytotoxicity on mouse fibroblast cells was measured as material for the production of natural anti-aging products.</p><p><strong>Materials and methods: </strong>This study investigates the antibacterial, antioxidant, and cytotoxic properties of coelomic fluid extracted from <i>Eisenia fetida/andrei</i>. Earthworms were cultured for a year, and their coelomic fluid was extracted using an electroshock method, sterilized, and lyophilized into powder. Antibacterial activity was tested against Escherichia coli and Staphylococcus aureus using MIC assays. Antioxidant properties were evaluated using the DPPH radical scavenging assay. Cytotoxicity effects on L929 and NHEK cell lines were assessed using MTT assays. Oxidative stress and enzymatic activities were analyzed by measuring malondialdehyde (MDA) levels and catalase activity in NHEK cells treated with coelomic fluid. A serum formulation incorporating coelomic fluid was prepared and subjected to stability tests, including pH, temperature, mechanical, and heavy metal residue analysis. Antibacterial and antioxidant properties of the serum were also evaluated. Statistical analyses were conducted using SPSS software (version 0.26). Results highlight the multifunctional potential of coelomic fluid for biomedical and cosmetic applications.</p><p><strong>Results: </strong>Coelomic fluid exhibited antibacterial activity with MICs of 0.15 mg/mL for both E. coli and S. aureus, showing significant inhibition at higher concentrations. Ciprofloxacin and penicillin demonstrated stronger effects compared to the coelomic fluid. Antioxidant activity increased with concentration, achieving 77% inhibition at 10 mg/mL, with an IC50 of 10.67 mg/mL. Cytotoxicity analysis revealed no significant toxicity below 20 mg/mL, with enhanced cell viability at 2.5-5 mg/mL and restorative effects on fibroblasts at 10 mg/mL. Oxidative stress assays indicated reduced lipid peroxidation and increased catalase activity without inducing significant oxidative stress. Measurement of residues of mercury and lead in the sera showed that they were less than 0.01 ppm for mercury and less than 0.03 and 0.05 ppm for lead, respectively. These levels are below the U.S. Food and Drug Administration's approved limits for these metals. Aqueous serum containing coelomic fluid showed similar antibacterial and antioxidant properties, emphasizing its potential for cosmetic and pharmaceutical applications.</p><p><strong>Conclusions: </strong>These results show that the use of earthworm coelomic fluid in skin care serum slows the aging process and restores damaged cells. The results of the present study can be considered as a patent.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":"281-295"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halo-tolerant Plant Growth Promoting Rhizobacteria Enhancing Mustard Seed Growth Under Salinity Stress for Sustainable Agriculture.","authors":"Kusum Sharma, Pawan Kumar Sharma, S Rajendra Prasad, Anupam Singh, Divya Jain, Sachidanand Singh","doi":"10.2174/0118722083354790250202145831","DOIUrl":"10.2174/0118722083354790250202145831","url":null,"abstract":"<p><strong>Introduction: </strong>Salinity is one of the primary environmental factors that significantly impact global crop production. Plant growth promoting rhizobacteria (PGPR) inoculation to crops improves the productivity of the crops.</p><p><strong>Methods: </strong>To develop a biofertilizer specifically for saline soil, bacteria were isolated from the rhizosphere of mustard plants along with the plant growth-promoting traits grown in saline soil (EC 6 dS m). Halotolerant 22 bacterial strains were isolated and identified from the rhizospheric soil mustard crop, Purvanchal (Indian state). According to the study, 54.54% of the isolates had phosphate solubilization efficiencies ranging from 7% to 27% on plate assays. According to quantitative measurements, 63.63% of the strains exhibited the ability to solubilize phosphate, with degrees of solubilization varying between 0.49 and 3.34 μg/ml. Furthermore, 50% of the isolates showed the ability to solubilize zinc, with solubilization rates varying from 12% to 53%. Further 59.09% of the bacterial strains showed ammonium production test; these strains were classified as having low (+), medium (++), and high (+++) levels of ammonium production.</p><p><strong>Results: </strong>According to the research, these halo-tolerant plant growth-promoting rhizobacteria (PGPR) have particular functional properties that may help mustard crops grow more rapidly in salinity-stressed environments. Because these PGPR strains increase nutrient availability and stimulate plant development, they may find use in agriculture, especially in saline settings.</p><p><strong>Conclusion: </strong>The study emphasizes how crucial it is to use PGPR with particular nutrient mobilization features to promote crop growth under difficult circumstances. The identification of these efficient strains may lead to the development of patent biofertilizers designed for saline soils, further supporting their application in modern agricultural practices.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":"20 1","pages":"180-193"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146228595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis, Characterization, Antibacterial and Anti-Biofilm Activity of CuO Nanoparticles using Ephedra major Plant Extract against <i>Pseudomonas aeruginosa</i>.","authors":"Pegah Shakib, Zahra Yari Talib, Asadullah Asadi, Zahra Najafi-Olya","doi":"10.2174/0118722083375049250507052242","DOIUrl":"10.2174/0118722083375049250507052242","url":null,"abstract":"<p><strong>Introduction: </strong>Nanoparticles are nanometer-sized particles that have unique properties and are used in various fields such as medicine, environment, and technology. The Ephedra major plant, with its medicinal properties, is a rich source for extracting molecules that can be used as agents for the biosynthesis of nanoparticles and improve their properties. The aim of the current study was the biosynthesis of copper oxide nanoparticles (CuONPs) using Ephedra major extracts, as well as the evaluation of their antibacterial and anti-biofilm activity against Pseudomonas aeruginosa.</p><p><strong>Materials and methods: </strong>The synthesis of CuO nanoparticles was performed using the aqueous extract of the leaves of Ephedra major plant .The synthesized nanoparticles were evaluated by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The well diffusion method investigated the antimicrobial activity of CuO-NPs synthesized against Pseudomonas aeruginosa. Then, the MIC and MBC of the synthesized nanoparticles were determined in 96-well microplates with different concentrations of CuO-NPs, aqueous extract, and chloramphenicol. The inhibition of Pseudomonas aeruginosa biofilm was investigated by staining with 1% crystal violet.</p><p><strong>Results: </strong>The results of the UV-Vis analysis showed that the absorption at the wavelength of 385 nm was the highest, which confirmed the formation of CuO-NPs. SEM and EDX results indicated that the nanoparticles formed in a spherical shape with an average size of 30 to 80 nm. Also, EDX analysis showed the presence of copper, carbon, and oxygen elements in nanoparticles. The CuO-PNs at the concentration of 2000 μg/ml exhibited a significant inhibitory effect against P. aeruginosa. Also, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of nanoparticles were 312 μg/ml. In addition, the results showed that CuO-NPs have an effect in inhibiting biofilm formation. The inhibitory effect against biofilm was greater with increasing concentration.</p><p><strong>Conclusion: </strong>The results of this study prove that CuO-NPs synthesized from the aqueous extract of Ephedra major plant can be used as an effective option in treating infections caused by P. aeruginosa and a new patent.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":"489-500"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patented Fibrinolytic Enzymes: An Overview.","authors":"Yuan Cao, Mingxuan Gou, Abu Zar, Anna Shestakova, Alexander Osmolovskiy","doi":"10.2174/0118722083364917250901064841","DOIUrl":"https://doi.org/10.2174/0118722083364917250901064841","url":null,"abstract":"<p><p>Fibrinolytic enzymes have garnered significant attention from researchers due to their immense potential in the medical field. As research continues to advance, the outcomes have become increasingly fruitful. The generation of applicable knowledge is usually accompanied by its protection through patent applications. This article compiles all patents related to \"fibrinolytic enzyme\" from Google Patents and the European Patent Office's Espacenet database, analyzing their core information, including publication year, application country, patent status, source of the fibrinolytic enzyme, and its various biochemical features. By combining relevant patent protection with current literature research, this article provides a novel and forward-looking summary of the current research status in the field of fibrinolytic enzymes.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145346916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Use of Different Oils and Emulsifiers in Oil-in-Water (O/W) Nanoemulsions.","authors":"Thalita da Cruz Monteiro Santana, Larissa Moraes Dos Santos Fonseca, Katharine Valéria Saraiva Hodel, Gabriele de Abreu Barreto, Danielle Devequi Gomes Nunes, Milca de Jesus SIlva, Bruna Aparecida Souza Machado","doi":"10.2174/0118722083398992251002045443","DOIUrl":"https://doi.org/10.2174/0118722083398992251002045443","url":null,"abstract":"<p><strong>Introduction: </strong>Nanoemulsions (NEs) are a biphasic systems formed by two immiscible liquids: (1) oil-in-water or (2) water-in-oil emulsions. These systems exhibit unique physicochemical properties due to their small particle size, making them more versatile compared to other emulsion systems. Thus, this research aimed to produce and evaluate oil-in-water nanoemulsions.</p><p><strong>Methods: </strong>NE's were prepared using Squalene, grape seed, and avocado oils, combined with soy lecithin or soy phosphatidylcholine as emulsifiers. The selection of oils considered both regulatory aspects and patent restrictions. Formulations were produced by microfluidization, and their physicochemical properties, such as particle size, polydispersity index, and zeta potential, were assessed at day one (D0) and day 7 (D7), under storage at 4°C, 25°C, and 40°C.</p><p><strong>Results: </strong>The nanoemulsions produced with soy phosphatidylcholine and vegetable oils exhibited good preliminary stability, due to their small particle size. In contrast, formulations using soy lecithin as the emulsifier did not show favorable results, especially the one with grape seed oil, which showed a large particle diameter. However, none of these formulations exhibited cellular cytotoxicity.</p><p><strong>Discussion: </strong>Emulsifier selection had a strong impact on NEs characteristics, with Soy- PC producing smaller and more uniform particles compared to soy lecithin. All NEs showed good biocompatibility in fibroblasts, indicating their safety.</p><p><strong>Conclusion: </strong>Among the formulations, the one with phosphatidylcholine demonstrated a safer and more reliable stability profile, making it a promising candidate for application in the pharmaceutical and cosmetics industries.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145346864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic Design of the Secreted form of SAG2 Protein as a Toxoplasma gondii Vaccine Candidate.","authors":"Arezoo Mesri, Negar Asadi, Hadi Maleki-Kakelar, Mortaza Taheri-Anganeh","doi":"10.2174/0118722083412016250925074449","DOIUrl":"https://doi.org/10.2174/0118722083412016250925074449","url":null,"abstract":"<p><strong>Introduction: </strong>Toxoplasmosis is one of the most common parasitic infections transmitted between humans and animals, caused by the protozoan Toxoplasma gondii. This organism acts as an obligate intracellular parasite, presenting an active and rapidly proliferating form known as a tachyzoite, in addition to two durable forms: the tissue cyst and the oocyst. Due to the considerable global impact of chronic infections in various host species, especially in humans and domestic animals, creating and producing an effective vaccine for preventing and managing toxoplasmosis is critically essential. The excretory antigens of Toxoplasma gondii play a vital role in vaccine development and diagnostic processes, with the SAG2 antigen being particularly significant. At present, sophisticated bioinformatics tools are utilized to design efficient signal peptides. This research will adopt an in silico approach to evaluating different signal peptides.</p><p><strong>Methods: </strong>The signal peptide sequence was obtained from the Signal Peptide website and Uniprot. Following this, SignalP was utilized to predict the signal peptides and determine the positions of their cleavage sites. The ProtProm tool was then employed to assess the physicochemical characteristics and solubility. Furthermore, ProtCompB was used to forecast the subcellular localization of the fusion protein.</p><p><strong>Results: </strong>Of the 50 signal peptides analyzed, 49 were excluded based on SignalP criteria. The signal peptide with the highest aliphatic index was HDEA_ECOLI, while TORT_ECOLI exhibited the highest GRAVY value.</p><p><strong>Discussion: </strong>Additionally, SAG2, when fused with all signal peptides, was explicitly designed for the Sec pathway, except NAPA_ECOLI, MBHT_ECOLI, CUEO_ECOLI, and OPGD_ECOLI.</p><p><strong>Conclusion: </strong>An appropriate signal peptide was recognized for the fusion and translocation of SAG2 into the extracellular environment. While additional experimental studies are necessary for conclusive validation.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145309022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demand Forecasting in the Tunisian Pharmaceutical Industry: A Comparative Study.","authors":"Mariem Mrad, Younes Boujelbene","doi":"10.2174/0118722083394111250929111803","DOIUrl":"https://doi.org/10.2174/0118722083394111250929111803","url":null,"abstract":"<p><strong>Introduction: </strong>Demand forecasting is critical for the pharmaceutical industry to ensure efficient production, inventory management, and distribution, especially in dynamic and competitive markets. This study addresses the challenges of accurate demand prediction within the Tunisian pharmaceutical sector.</p><p><strong>Methods: </strong>The primary aim was to compare the forecasting accuracy of two methods, Holt-Winters (HW) and Multilayer Perceptron (MLP) neural networks, for three drug categories: Antiviral, Antibiotic, and Pain Relief. Additionally, the study provides actionable recommendations to enhance forecasting strategies. A 24-month (n=24) historical sales dataset (October 2020 to September 2022) from a Tunisian pharmaceutical demand forecasting company was analyzed. The analysis utilized the Holt-Winters model to incorporate seasonal adjustments and an MLP neural network to capture complex, non-linear sales patterns.</p><p><strong>Results: </strong>Both models were evaluated using metrics such as mean squared error (MSE) to quantify prediction accuracy. The MLP neural network consistently and significantly outperformed the Holt-Winters method, demonstrating markedly lower MSE values (e.g., 0.0206 for Antivirals, compared to 30.06 for HW) and greater adaptability to demand variability across all drug categories.</p><p><strong>Discussion: </strong>While effective for seasonal patterns, HW struggled with irregular fluctuations and complex dynamics. This study highlights the superiority of MLP neural networks for pharmaceutical demand forecasting due to their adaptability and accuracy in handling non-linear and variable data.</p><p><strong>Conclusion: </strong>The findings provide a strong quantitative basis for Tunisian pharmaceutical companies to adopt advanced machine learning techniques for more reliable planning. However, to fully realize the potential of these techniques and address the limitations outlined in this research, future research should explore hybrid models and integrate extended datasets that incorporate external market dynamics and trends.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145293414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Molecular Biomarkers in E. coli-Induced Urinary Tract Infections: Insights into the Long Non-Coding RNAs Contribution to Antimicrobial Resistance.","authors":"Randa Nasr, Sami Mohamed Nasr, Hend Okasha, Heba Dahroug, Mohamed Khaled Ibrahim","doi":"10.2174/0118722083388214250924193840","DOIUrl":"https://doi.org/10.2174/0118722083388214250924193840","url":null,"abstract":"<p><strong>Introduction: </strong>Urinary Tract Infections (UTIs) caused by Escherichia coli are a major concern due to rising antimicrobial resistance. Long non-coding RNAs (lncRNAs) play crucial roles in gene regulation, but their involvement in UTIs and their implication in antimicrobial resistance mechanisms are not well understood. This study investigates the association between specific long non-coding RNAs, immune response, and antibiotic resistance in patients with infections caused by E. coli.</p><p><strong>Methods: </strong>Quantification of specific lncRNAs (NEAT1-1, NEAT1-2, MIR3142HG, AK170409, and IL7AS) was performed using quantitative PCR. Inflammatory markers IL-1β, IRF3, and NF-κB were measured in UTI patients using ELISA kits to assess their biological response. The minimum inhibitory concentration of eleven antibiotics was determined by testing all 25 urine samples and categorized as sensitive, intermediate, or resistant.</p><p><strong>Results: </strong>NEAT1-1, NEAT1-2, MIR3142HG, and AK170409 were significantly upregulated (p < 0.05). Inflammatory markers were significantly elevated in all samples: IL-1β at 72.36 ± 13.8 pg/mL, IRF3 at 79.36 ± 15.01 pg/mL, and NF-κB at 4.43 ± 0.82 pg/mL (p < 0.0001). NEAT1-2 and AK170409 expression correlated with distinct antibiotic response patterns (p < 0.05). Differences in biological and hematological data were observed among UTI patients with varying topographic expressions of specific long noncoding RNAs (lncRNAs).</p><p><strong>Discussions: </strong>Specific lncRNAs may be involved in modulating immune responses and influencing antibiotic susceptibility in UTIs. Their expression patterns reflect both the severity of infection and resistance profiles, suggesting a functional role in the development of antimicrobial resistance.</p><p><strong>Conclusion: </strong>LncRNAs are potential biomarkers for UTIs caused by E. coli. Future studies should focus on elucidating the precise role of lncRNAs in the development of antibiotic resistance in these infections.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145286795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic Assessment of Bio-oil Production from Algae: A Bibliometric Analysis.","authors":"Radhika Jaithaliya, Divya Jain, Dipjyoti Chakraborty, Kakoli Dutt","doi":"10.2174/0118722083384019250805103219","DOIUrl":"https://doi.org/10.2174/0118722083384019250805103219","url":null,"abstract":"<p><strong>Introduction: </strong>The production of bio-oil from microalgae is gaining attention as an alternative renewable energy source. To generate advances in this field, it is essential to identify the gaps in existing research. Overcoming this barrier necessitates addressing methodologies that can assess the existing work and develop relationships between publications, research groups, and their impact.</p><p><strong>Methods: </strong>To chart a course toward practicality, this study employs bibliometric indicators, leveraging tools such as R Studio, VOSviewer 1.6.19, and Biblioshiny for network visualization and analysis. Drawing on the Scopus database spanning 2004-2024, a meticulous keyword strategy effectively segregated relevant papers.</p><p><strong>Results: </strong>The findings suggest that the total number of published documents is 1005. The publication trend on this topic shows a significant increase since 2008, with a record high of 107 publications in 2022. China dominates the publication list, with thirteen universities contributing the highest. In the context of algal bio-oil, a total of 2850 authors contributed to 1005 publications.</p><p><strong>Discussion: </strong>A three-way map displaying the relationships between journal titles, paper titles, and author keywords provides useful insights into the research landscape of the field. Algal research is growing and collaborating, despite bibliometric limitations such as time dependency and database scope. Such studies, however, offer valuable insights into the evolving bio-oil field.</p><p><strong>Conclusion: </strong>The study aims to link sustainable economic development with technological innovation and related economic growth. Its objective is to provide researchers with information on niche research clusters, quantify emerging methodologies, facilitate collaborative networks, and identify knowledge gaps, while assisting in determining the impact of specific publication venues. In addition to analyzing key economic drivers, the study will also recommend prospective research themes to disseminate algae-based biooil production research.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Novel Microorganisms for PAH Degradation in Egyptian Soil: A Bioremediation Strategy for Soil Detoxification.","authors":"Amira A Shendy, Heba K Abdelhakim, Mohamed A El-Desouky, Fatma B Rashidi","doi":"10.2174/0118722083393750250630081655","DOIUrl":"https://doi.org/10.2174/0118722083393750250630081655","url":null,"abstract":"<p><strong>Introduction: </strong>Polycyclic aromatic hydrocarbons (PAHs) are toxic petroleum byproducts in soil, exhibiting significant genotoxic properties. Microorganisms residing in contaminated soils serve as effective detoxifying agents. Among various strategies, bioremediation is an efficient biological method for detoxifying PAHs.</p><p><strong>Method: </strong>Hundreds of soil samples were collected from the Gulf of Suez, Egypt. The isolation process utilized an enrichment culture system with phenol naphthalene (PN) (10 mg/mL) as the primary carbon source. HPLC analysis was applied to confirm PN degradation. Consequently, the bacterial strain was characterized morphologically, biochemically, and through partial sequencing of its 16S rRNA gene. Subsequently, its plasmid was purified to transfer its phenotype to Escherichia coli. Finally, a bioremediation approach was conducted to test its PAH degradation.</p><p><strong>Results: </strong>HPLC analysis was performed to confirm PN degradation by the isolated strain. The isolated strain was identified as Lysinibacillus species AAS1 (OR044755.1) with 98.43% sequence similarity to the Lysinibacillus genus. Subsequently, E. coli transformants with the isolated plasmid were grown in the presence of PN as the primary carbon source. Finally, the bioremediation assay of the isolated strain exhibited a high efficiency in detoxifying PN.</p><p><strong>Discussion: </strong>The novel identified Lysinibacillus species AAS1 (OR044755.1) shows promise for PAHs detoxification, which may lead to the exploration of a biological agent for the remediation of water and soil contaminated with PAHs.</p><p><strong>Conclusion: </strong>A novel bacterial strain bearing a plasmid that can degrade PN was isolated from Egyptian petroleum waste-contaminated soil. It paved the way for further studies to isolate the whole gene(s) responsible for such degradation.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}