Recent patents on biotechnology最新文献

{"title":"Economic Assessment of Bio-oil Production from Algae: A Bibliometric Analysis.","authors":"Radhika Jaithaliya, Divya Jain, Dipjyoti Chakraborty, Kakoli Dutt","doi":"10.2174/0118722083384019250805103219","DOIUrl":"https://doi.org/10.2174/0118722083384019250805103219","url":null,"abstract":"<p><strong>Introduction: </strong>The production of bio-oil from microalgae is gaining attention as an alternative renewable energy source. To generate advances in this field, it is essential to identify the gaps in existing research. Overcoming this barrier necessitates addressing methodologies that can assess the existing work and develop relationships between publications, research groups, and their impact.</p><p><strong>Methods: </strong>To chart a course toward practicality, this study employs bibliometric indicators, leveraging tools such as R Studio, VOSviewer 1.6.19, and Biblioshiny for network visualization and analysis. Drawing on the Scopus database spanning 2004-2024, a meticulous keyword strategy effectively segregated relevant papers.</p><p><strong>Results: </strong>The findings suggest that the total number of published documents is 1005. The publication trend on this topic shows a significant increase since 2008, with a record high of 107 publications in 2022. China dominates the publication list, with thirteen universities contributing the highest. In the context of algal bio-oil, a total of 2850 authors contributed to 1005 publications.</p><p><strong>Discussion: </strong>A three-way map displaying the relationships between journal titles, paper titles, and author keywords provides useful insights into the research landscape of the field. Algal research is growing and collaborating, despite bibliometric limitations such as time dependency and database scope. Such studies, however, offer valuable insights into the evolving bio-oil field.</p><p><strong>Conclusion: </strong>The study aims to link sustainable economic development with technological innovation and related economic growth. Its objective is to provide researchers with information on niche research clusters, quantify emerging methodologies, facilitate collaborative networks, and identify knowledge gaps, while assisting in determining the impact of specific publication venues. In addition to analyzing key economic drivers, the study will also recommend prospective research themes to disseminate algae-based biooil production research.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Novel Microorganisms for PAH Degradation in Egyptian Soil: A Bioremediation Strategy for Soil Detoxification.","authors":"Amira A Shendy, Heba K Abdelhakim, Mohamed A El-Desouky, Fatma B Rashidi","doi":"10.2174/0118722083393750250630081655","DOIUrl":"https://doi.org/10.2174/0118722083393750250630081655","url":null,"abstract":"<p><strong>Introduction: </strong>Polycyclic aromatic hydrocarbons (PAHs) are toxic petroleum byproducts in soil, exhibiting significant genotoxic properties. Microorganisms residing in contaminated soils serve as effective detoxifying agents. Among various strategies, bioremediation is an efficient biological method for detoxifying PAHs.</p><p><strong>Method: </strong>Hundreds of soil samples were collected from the Gulf of Suez, Egypt. The isolation process utilized an enrichment culture system with phenol naphthalene (PN) (10 mg/mL) as the primary carbon source. HPLC analysis was applied to confirm PN degradation. Consequently, the bacterial strain was characterized morphologically, biochemically, and through partial sequencing of its 16S rRNA gene. Subsequently, its plasmid was purified to transfer its phenotype to Escherichia coli. Finally, a bioremediation approach was conducted to test its PAH degradation.</p><p><strong>Results: </strong>HPLC analysis was performed to confirm PN degradation by the isolated strain. The isolated strain was identified as Lysinibacillus species AAS1 (OR044755.1) with 98.43% sequence similarity to the Lysinibacillus genus. Subsequently, E. coli transformants with the isolated plasmid were grown in the presence of PN as the primary carbon source. Finally, the bioremediation assay of the isolated strain exhibited a high efficiency in detoxifying PN.</p><p><strong>Discussion: </strong>The novel identified Lysinibacillus species AAS1 (OR044755.1) shows promise for PAHs detoxification, which may lead to the exploration of a biological agent for the remediation of water and soil contaminated with PAHs.</p><p><strong>Conclusion: </strong>A novel bacterial strain bearing a plasmid that can degrade PN was isolated from Egyptian petroleum waste-contaminated soil. It paved the way for further studies to isolate the whole gene(s) responsible for such degradation.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Status of Microalgae-based Food Products: Future Trends of Functional Ingredients.","authors":"Marcele Leal Nörnberg, Luisa Chitolina Schetinger, Tatiele Casagrande do Nascimento, Pricila Nass Pinheiro, Patricia Acosta Caetano, Eduardo Jacob-Lopes, Leila Queiroz Zepka","doi":"10.2174/0118722083380758250802021828","DOIUrl":"https://doi.org/10.2174/0118722083380758250802021828","url":null,"abstract":"<p><p>The use of microalgae in food and beverages is becoming increasingly popular as a viable way to develop products with enhanced nutritional profiles, offering positive health effects. In parallel, the plant-based food market is expanding due to the growing vegan, vegetarian, and flexitarian populations, prompting manufacturers to create innovative foods and techniques, such as the addition of microalgae to products. These functional and/or nutraceutical foods present an attractive option for consumers seeking plantbased alternatives. Although some challenges remain, this is a growing market. Furthermore, biotechnological processes are being utilized to optimize the production of microalgae with even more robust nutritional characteristics, thereby increasing their added value. This review was based on a structured literature search across major databases, applying predefined keywords and selection criteria to identify recent advances, regulatory aspects, and biotechnological developments in the field. These innovations hold significant potential to meet the rising demand for bioactive products and to propel a new era in the commercialization of microalgae-based products, a segment still underexplored in the current market.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144856158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Kaempferol against Biofilm Formation by Klebsiella pneumoniae Clinical Isolates.","authors":"Radwa Hamdy Abd Allah, Safia Samir, Sami Mohamed Nasr, Mohamed Khaled Ibrahim","doi":"10.2174/0118722083386487250804015300","DOIUrl":"https://doi.org/10.2174/0118722083386487250804015300","url":null,"abstract":"<p><strong>Background: </strong>Biofilm production is a key factor in the development of antibiotic resistance in multidrug-resistant Klebsiella pneumoniae (K. pneumoniae), a significant contributor to healthcare-associated infections (HAIs). Kaempferol, a flavonoid, is widely recognized for its ability to combat various microorganisms.</p><p><strong>Aim: </strong>Our goal is to assess the impact of kaempferol on K. pneumoniae biofilms by determining the level of gene expression for the biofilm-forming genes.</p><p><strong>Methods: </strong>Fifty K. pneumoniae isolates were studied. Different doses of kaempferol with a concentration range of 0.04 to 100% in Luria Bertani broth (LB) medium were incubated at 37℃ for 24 h with forty-three K. pneumoniae strong and intermediate biofilm producers. The minimum inhibitory concentration (MIC) of kaempferol was determined. Molecular detection of the biofilm-forming genes (mrkA, pgaA, wbbM, and wzm) was performed on all isolates before and after kaempferol treatment at 0.5 x MIC.</p><p><strong>Results: </strong>Seven isolates out of 50 (14%) exhibited weak biofilm formation ability, 6 out of 50 (12%) were moderate producers, and 37 out of 50 (74%) were strong producers. The MIC values of kaempferol for K. pneumoniae ranged from 50% to 6.25% (p = 0.0003). The levels of expression of the studied genes were slightly decreased after treatment compared with their corresponding values before treatment.</p><p><strong>Conclusion: </strong>Based on current knowledge, few research studies have investigated the impact of kaempferol on K. pneumoniae biofilms. Our results show that its effect on the biofilms of this bacterium is moderate to weak. Further research is necessary to determine potential synergies with other treatments.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144856159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Activity of Leaf and Root Parts of Cenchrus biflorus Roxb.","authors":"Swati Chaudhary, Rajabrata Bhuyan, Divya Jain, Anand Prakash","doi":"10.2174/0118722083390656250630080441","DOIUrl":"https://doi.org/10.2174/0118722083390656250630080441","url":null,"abstract":"<p><strong>Introduction: </strong>Medicines and herbal formulations are derived from different parts of medicinal plants, which are the best-known sources for treating various diseases. This research focuses on assessing the antimicrobial potential of crude extracts from the leaves and roots of Cenchrus biflorus Roxb.</p><p><strong>Methods: </strong>Methanol, hydroethanol (50:50), and aqueous extracts were obtained using the Soxhlet extraction method. The disc diffusion method was used to study the antimicrobial activity of the extracts against a variety of test microorganisms, including bacteria (Escherichia coli and Bacillus subtilis) and fungus (Aspergillus niger). The disc diffusion method was used to assess bacterial susceptibility, revealing the potent inhibitory effect of the methanol extract on E. coli. All extracts demonstrated significant antimicrobial activity against various microorganisms.</p><p><strong>Results: </strong>Remarkably, methanol extract of leaf demonstrated the highest antibacterial activity, with a 16.3 ± 1.78 mm zone of inhibition (ZOI) with Activity Index (AI) of 0.875, and a Relative Percentage Inhibition (RPI) of 80 against E. coli, followed by Bacillus subtilis (ZOI = 15.5 ± 1.31 mm, AI = 0.869, RPI = 78.57). The methanol extract of the root showed strong antifungal activity against Aspergillus niger (with a 12.9 ±1 mm ZOI, AI = 0.636, and RPI = 42.85), while the water extract of the root displayed 7.8 mm inhibition zones.</p><p><strong>Discussion: </strong>Methanol and hydroethanol extracts of the leaf and root exhibited strong inhibitory effects against selected microbial strains. Each plant solvent extract suppressed microbial development in a distinct manner, and methanol and hydroethanol extracts inhibited microbial development more efficiently than aqueous extracts. Interestingly, water extracts had the least effective inhibitory effects across all strains. Notably, water extracts showed the weakest inhibitory effects against all strains.</p><p><strong>Conclusion: </strong>The current study demonstrated the efficacy of crude extracts of Cenchrus biflorus Roxb. against the tested strains of bacteria and fungi and also discussed their potential application as antibacterial agents for combating infectious diseases.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Multifaceted Health Benefits of Natural Dietary Sources: Antimicrobial, Antioxidant, Anticancer, and Antidiabetic Properties.","authors":"Preeti Kaushik, Anjela Gahalayan, Sonia Parashar, Madhu Rani, Neha Khurana, Spikey Dogra, Parijat Pandey","doi":"10.2174/0118722083375459250801073140","DOIUrl":"https://doi.org/10.2174/0118722083375459250801073140","url":null,"abstract":"<p><p>The lifestyle of today's generation contributes to various health issues like cancer, diabetes, obesity, heart disease, and high blood pressure. A significant factor contributing to these harmful lifestyle choices is the overconsumption of highly processed, energy-dense foods that are rich in saturated and trans fats, sodium, and added sugars, Conversely, adopting healthier dietary patterns that prioritize the intake of fruits, vegetables, whole grains, lean proteins, and healthy fats has been shown to protect against these chronic health conditions. Investigating the diverse health benefits of natural food sources requires a holistic approach encompassing dietary intake evaluations, laboratory and animal studies, and human clinical research. These investigations examine the antimicrobial, antioxidant, cancer-fighting, and blood sugar-regulating properties of compounds derived from plants. Studies indicate that diets abundant in whole grains, fruits, and vegetables supply crucial nutrients and biologically active substances such as polyphenols and flavonoids, which provide protective benefits against long-term disease conditions, including heart disease, cancer, and diabetes. Consequently, it is imperative to adjust our dietary practices and lifestyle choices to mitigate the risk of various ailments. Naturally occurring compounds such as curcumin, quercetin, kaempferol, and resveratrol, which are found in diverse food sources, have the potential to combat numerous diseases when incorporated into our diets. This review explores an array of compounds present in dietary sources and their associated biomedical properties, including their anticancer, antidiabetic, antioxidant, and antimicrobial effects. Furthermore, it explores various dietrelated strategies designed to promote a healthier lifestyle, including the incorporation of a diverse range of fruits, vegetables, and spices rich in polyphenolic compounds into one's daily nutritional intake.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Blockchain Technology in the Improvement of Supply Chain Management in the Pharmaceutical Industry.","authors":"Dinesh Kumar, Rajni Tanwar, Debarshi Kar Mahapatra, Nisham Lohan, Geetanjali Dahiya, Divya Jain","doi":"10.2174/0118722083347810250711232759","DOIUrl":"https://doi.org/10.2174/0118722083347810250711232759","url":null,"abstract":"<p><p>Blockchain technology has drawn a lot of interest in the healthcare industry in recent years. Efficient data management is crucial for pharmacies. Blockchain technology is a novel technology that has the potential to make many pharmaceutical processes safer and more transparent. In 2013, the US Congress created the Drug Supply Chain Security Act [DSCSA] to prevent the distribution of stolen, contaminated, or counterfeit drugs. Blockchain technology serves as an answer to this problem as it enables the tracking and tracing the product from manufacturer to patient through an electronic, immutable, digitized tracking record of all steps from inventory to consumer in the drug supply chain. To ensure the safety of a peer-to-peer network of transactions, blockchain technology employs cryptographic methods to create a distributed ledger that is not centralized. Incorporating it into a product ensures its safety and marketability. Blockchain technology offers solutions to enhance the validity, reliability, and efficiency of medication manufacturing by facilitating easy compliance with legislation. Additionally, it offers an opportunity to address a major problem: the proliferation of counterfeit drugs that enter the system and ultimately reach consumers. The pharmaceutical industry's deficient supply chain management results in counterfeit and fake drug entering the supply chain and negatively affecting patients' health, leading to serious complications or even death. Supply chain management may benefit from blockchain technology's transparency and immutability at every step of the process, which increases confidence, security, traceability, and transparency. Blockchain technology, designed to manage electronic data, holds promise for enhancing clarity. Every user of a computer network has access to the same immutable record of transactions known as a blockchain.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144744550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Patents of Stent Grafts for Intravascular Aortic Repair.","authors":"Yiwen Wang, Yuxin Zhang","doi":"10.2174/0118722083355380250716073539","DOIUrl":"https://doi.org/10.2174/0118722083355380250716073539","url":null,"abstract":"<p><strong>Introduction: </strong>Endovascular aortic repair involves the placement of stents through minimally invasive methods to seal rupture sites near the aortic inflow tract, thereby preventing blood entry into the false lumen and promoting thrombosis, which reduces the risk of aortic rupture. Endovascular stents typically consist of a metal framework and a flexible membrane graft designed to reopen obstructed aortic segments and maintain blood flow through the true lumen. Consequently, stents are widely used to treat aortic expansion diseases and aortic occlusive stenosis. However, traditional stents have limitations in terms of adaptability to complex anatomical structures, long-term durability, biomechanical stability, and reliance on radial support force for fixation, lacking active fixation mechanisms. These shortcomings remain the primary causes of postoperative complications, significantly impacting the quality of life for patients with aortic dissection.</p><p><strong>Methods: </strong>The research status of the endovascular stent was discussed in depth, and the main factors for the optimal design of the stent (geometry, pattern configuration, additional fixtures, and optimization methods) were analyzed and summarized according to the complications targeted by the repair device.</p><p><strong>Results: </strong>The composition structure, working principle, and development status of the stent grafts under review are elaborated in detail. Stent grafts attempt to alleviate postoperative complications through three approaches: enhancing the flexibility of the stent framework, improving the fit between the vessel wall and the stent, and reducing vascular injury. Blood flow guiding channels are established to alleviate the obstruction of branch blood flow. Additional self-anchoring devices are added to adapt to the dynamic remodeling of blood vessels.</p><p><strong>Discussion: </strong>The effects of various factors, including geometric parameters, structural design, and parameter optimization techniques, on the optimization of stent primary mechanical performance are discussed. The current research status of functional improvement methods for stents is also summarized.</p><p><strong>Conclusion: </strong>Refining the quantitative relationship between stent structural parameters and mechanical performance, as well as exploring the balance criteria between flexibility and radial support force, represent promising directions for future development. These objectives necessitate further in-depth analysis and research.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Potential to Reality: Unraveling the Factors Limiting the Use of Microalgae as Sustainable Blue Food Protein Sources - A Critical Review.","authors":"Luisa Chitolina Schetinger, Marcele Leal Nörnberg, Tatiele Casagrande do Nascimento, Leila Queiroz Zepka, Eduardo Jacob-Lopes","doi":"10.2174/0118722083358276250703100020","DOIUrl":"https://doi.org/10.2174/0118722083358276250703100020","url":null,"abstract":"<p><p>Microalgae are promising and sustainable sources of blue food proteins, offering high nutritional quality, environmental resilience, and the potential to meet the rising demand for alternative proteins. Despite these advantages, several challenges hinder their large-scale adoption, including production costs, regulatory barriers, protein extraction difficulties, and consumer perception. This review explores the key factors limiting the use of microalgae in the food industry, addressing economic and technological feasibility, regulatory aspects, and consumer acceptance. The analysis includes commonly used microalgae species, their nutritional profiles, and strategies for optimizing their incorporation into food products. Moreover, developing circular biorefineries and utilizing industrial wastewater for cultivation presents a viable solution to reduce costs and enhance sustainability. Additionally, advancements in protein extraction techniques, combined with technological innovations such as microencapsulation, may overcome sensory challenges, expanding consumer acceptance of microalgae-enriched products. Raising consumer awareness of the nutritional and environmental benefits of microalgae is also crucial for market adoption. Given the global need for sustainable food sources, microalgae represent a viable alternative but require scientific, regulatory, and strategic advancements to become a widely adopted solution in the alternative protein industry.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Biosurfactant Isolated from Actinobacteria on the bfp Gene Expression of Aeromonas hydrophila, Isolated from Children's Stool Samples.","authors":"Sina Ghobadi, Pegah Shakib, Ashkan Dirbaziyan, Mohsen Zargar","doi":"10.2174/0118722083366860250623142119","DOIUrl":"https://doi.org/10.2174/0118722083366860250623142119","url":null,"abstract":"<p><strong>Introduction: </strong>Aeromonas hydrophila, a rod-shaped, gram-negative bacterium, is frequently found in aquatic surroundings and additionally present in drinking water, sewage, and food sources. This microbe is gaining recognition as a potential threat to health, classifying it as an emerging pathogen. Biosurfactants are microbial-derived compounds that share hydrophilic and hydrophobic moieties that are surface active. This study aimed to investigate the effect of biosurfactant isolated from Actinobacteria on the expression of the bfp gene of A. hydrophila isolated from the stool samples of children referred to Hazrat Masoumeh Hospital in Qom, Iran, from May 2022 to March 2023.</p><p><strong>Materials and methods: </strong>Actinobacteria were isolated from soil samples of the desert areas of Qom province, Iran. Biochemical and molecular tests of 16S rRNA were used to identify Actinobacteria isolates. The produced biosurfactant was investigated by methods of hemolysis, oil droplet destruction, lipase production, oil expansion, emulsifying activity, and surface tension reduction measurement. The structure of biosurfactant was investigated by Fourier transform infrared spectroscopy (FTIR) analysis, and its effect on bfp gene expression was measured. Also, isolates of A. hydrophila were obtained from stool samples of children referred to Hazrat Masoumeh Hospital in Qom from May 2022 to March 2023. Then, the effect of a biosurfactant isolated from Actinobacteria on the bfp gene expression of A. hydrophila isolates was measured by RT-PCR.</p><p><strong>Results: </strong>Based on sequencing data, the Streptomyces genus with the ability to produce biosurfactant was isolated from the soil of the studied area, which could reduce the expression of the bfp gene after treatment with biosurfactant in clinical isolates of A. hydrophila.</p><p><strong>Conclusion: </strong>The findings of the present study show that the desert soils of Qom province are a potential area for finding actinobacterial isolates with the ability to produce biosurfactants and influence the expression of pathogenic genes of clinical isolates of A. hydrophila.</p>","PeriodicalId":21064,"journal":{"name":"Recent patents on biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}