Recent patents on food, nutrition & agriculture最新文献

{"title":"Whole Grains, Dietary Fibers and the Human Gut Microbiota: A Systematic Review of Existing Literature.","authors":"Hadith Tangestani,&nbsp;Hadi Emamat,&nbsp;Hamid Ghalandari,&nbsp;Sakineh Shab-Bidar","doi":"10.2174/2212798411666200316152252","DOIUrl":"https://doi.org/10.2174/2212798411666200316152252","url":null,"abstract":"<p><strong>Background: </strong>The health benefits of dietary fibers have been proved for a long time. The importance of microbiota has been identified in human health and there is a growing interest to study the factors affecting it.</p><p><strong>Objective: </strong>This systematic review aimed to investigate the impact of fiber and whole grains (WGs) on human gut microbiota in a patent-based review.</p><p><strong>Methods: </strong>All related clinical trials were systematically searched on PubMed and Scopus search engines from inception up to Feb 2020. Interventional human studies reporting changes in microbiota by using any type of grains/fibers were included. The following information was extracted: date of the publication, location and design of the study, sample size, study population, demographic characteristics, the amount of dietary WGs/fiber, the duration of intervention, the types of grains or fibers, and changes in the composition of the microbiota.</p><p><strong>Results: </strong>Of 138 studies which were verified, 35 studies with an overall population of 1080 participants, met the inclusion criteria and entered the systematic review. The results of interventional trials included in this review suggest some beneficial effects of consuming different amounts and types of WGs and fibers on the composition of intestinal microbiota. Most included studies showed that the intake of WGs and fibers increases bifidobacteria and lactobacilli and reduces the pathogenic bacteria, such as Escherichia coli and clostridia in the human gut.</p><p><strong>Conclusion: </strong>The consumption of WGs/fibers may modify the intestinal microbiota and promote the growth of bifidobacteria and lactobacilli. Nevertheless, further research is warranted in different populations and pathological conditions.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 3","pages":"235-248"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37742324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization Growth of Spirulina (Arthrospira) Platensis in Photobioreactor Under Varied Nitrogen Concentration for Maximized Biomass, Carotenoids and Lipid Contents.","authors":"Hanaa H Abd El Baky,&nbsp;Gamal S El Baroty,&nbsp;Enas M Mostafa","doi":"10.2174/2212798410666181227125229","DOIUrl":"https://doi.org/10.2174/2212798410666181227125229","url":null,"abstract":"<p><strong>Aims and background: </strong>Spirulina (Arthrospira) platensis (SP) microalgae were cultured in Zarrouk Medium (ZM), containing three nitrogen concentrations (N-limited, N-optimal and Nrich medium) in ten liter-photo-bioreactor (10 L PBR) for 15-days, in order to study changes in lipid compounds (total carotenoids and total lipids and their effect on fatty acid profile). Based on US patent, the yield of bioactive compounds (such as gamma-linolenic acid GLA, C18:3) extracted from microalgae biomass, mainly depends on the extraction processes (1). GLA has much attention with respect to its therapeutic properties such as its ability to decrease blood cholesterol levels.</p><p><strong>Methods: </strong>The impact of the addition of N in cultures of S. platensis in terms of growth, biomasses and induced lipid compounds (total carotenoids and total lipid contents and its fatty acid profile), as well as the Sonication (SON) and Microwave (MIC) process as aiding techniques for lipid extraction compared with a Cold Condition (COL), was examined. GC/MS method was used to determine the fatty acid profile of lipid extract of SP cultures.</p><p><strong>Results: </strong>In all S. platensis tested culture, the SP was growing successfully, with varying degrees. In N-rich media, the highest cell growth rate and biomass yield were obtained compared with that recorded in other cultures. Under an N-limited condition, SP had higher Total Carotenoids (TCAR, 45.54 mg/g dw) and total lipid contents (TL, 29.51%± 1.92 g/100g dw) compared with that recorded either in N-rich (11.2 mg/g dw) or in N-optimal (6.23 mg/g dw) cultures. Thus, SP copes with the N -stress by altering the metabolic pathways towards inducing lipid biosynthesis. To maximize the TL and TCAR extraction yields, from N-limited cultures, a set of operating process was applied including the Sonication (SON) and Microwave (MIC), which were used as aiding techniques for lipid extraction compared with the Cold Condition (COL) techniques. The results showed that the extraction efficiency of the S. platensis TL increased in the following order: MIC (29.51%± 1.92) > SON (25.46% ± 1.65> COL (20.43% ±1.43). In a comparative study for its fatty acid profiles (FAPs) among all SP cultures, lipids were analyzed by GC/MS. The predominant fatty acids (>10%, of total FA) were found to be myristic acid (C14:0, MA), palmitic acid (C16:0, PA) and oleic acid (C18:1).</p><p><strong>Conclusion: </strong>The study concluded that the N-limited condition was found to have a strong influence on biomass dry weight and lipid contents and total carotenoids in SP cells compared to either Nrich or N-optimal conditions. The use of sonication and the microwave techniques lead to a great increase in the extraction of lipid contents and in high amount Polyunsaturated Fatty Acids (PUFAs) in N-limited cultures, in particular, the omega-6 (ω 6) and omega-3 (ω 3) of the essential C18 fatty acids. It seems that the SP rich in lipid c","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 1","pages":"40-48"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666181227125229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36818138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Fungal Diversity and their Interaction with Plants for Agriculture Sustainability Under Stressful Condition.","authors":"Muhammad Ikram,&nbsp;Niaz Ali,&nbsp;Gul Jan,&nbsp;Farzana G Jan,&nbsp;Naeem Khan","doi":"10.2174/2212798410666190612130139","DOIUrl":"https://doi.org/10.2174/2212798410666190612130139","url":null,"abstract":"<p><p>Endophytic fungi are an interesting group of organisms that colonize the healthy internal tissues of living plants, and do not cause any symptoms of disease in the host plants. Several decades of study and research have rustled the co-existing endophytes with their host plants, which can significantly influence the formation of metabolic products in plants, as they have the ability to produce a new interesting bioactive compound, which is of pharmaceutical, industrial and agricultural importance. Empirical evidences have indicated that endophytic fungi can confer profound impacts on plant communities by enhancing their growth, increasing their fitness, strengthening their tolerance to abiotic and biotic stresses, enhancing the defense mechanism and promoting the accumulation of secondary metabolites that provide immunity against pathogens. Many of these compounds are novel products and could be granted patents. Further, there are growing interests of multinational companies using these patents prepared in special formula to sell in international markets. This review addresses biodiversity and biological roles of endophytic fungi in association with their host plants through reviewing published research data obtained from the last 30 years and highlights their importance for plants, industry as well as ecosystem.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 2","pages":"115-123"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666190612130139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37066087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cocoa Pulp as Alternative Food Matrix for Probiotic Delivery.","authors":"Gabriele M Guimarães,&nbsp;Letícia A Soares,&nbsp;Tuânia N L Silva,&nbsp;Iracema L Q de Carvalho,&nbsp;Helder M S Valadares,&nbsp;George A Sodré,&nbsp;Daniel B Gonçalves,&nbsp;Elisabeth Neumann,&nbsp;Flávio G Da Fonseca,&nbsp;Gabriel Vinderola,&nbsp;Paulo A Granjeiro,&nbsp;Juliana T de Magalhães","doi":"10.2174/2212798410666190408151826","DOIUrl":"https://doi.org/10.2174/2212798410666190408151826","url":null,"abstract":"<p><strong>Background: </strong>For screening probiotic strains with viability and stability in non-dairy foods for health benefits, we revised all patents relating to probiotics in food.</p><p><strong>Objective: </strong>Screening of potential probiotics from Brazilian Minas artisanal cheese and verify their survival in frozen Brazilian cocoa pulp.</p><p><strong>Methods: </strong>Isolation and identification of the strains. The potential probiotic characterization involved gastric juice and bile resistance, antibiotic and antimicrobial activity, hydrophobicity, autoaggregation, coaggregation and adhesion assay in HT-29 cells. Organoleptic, viability and stability of probiotic strain in frozen cocoa pulp were evaluated.</p><p><strong>Results: </strong>Fourteen strains of Lactobacillus plantarum (9), Weissella paramesenteroides (3), Lactobacillus fermentum (1), and Leuconostoc mesenteroides (1) were obtained. Most of the strains were resistant to simulated gastric acidity and bile salts. Almost all strains were sensitive to the antibiotics tested, except to ciprofloxacin and vancomycin. About 47% of the strains are potential producers of bacteriocins. High hydrophobicity was observed for four strains. Autoaggregation ranged from 8.3-72.6% and the coaggregation capacity from 5.2-60.2%. All of the assessed strains presented more than 90% of adhesion to HT-29 intestinal cells. The percentage of Salmonella inhibition in HT-29 cells ranged from 4.7-31.1%. No changes in color, aroma, and pH were observed in cocoa pulps after storage at -20 °C for 90 days.</p><p><strong>Conclusion: </strong>Wild strains of acid lactic bacteria from cheese proved to be viable and stable in frozen Brazilian cocoa pulp. This work showed a promising application of L. plantarum isolated strains to be used with frozen cocoa pulp matrix in probiotics food industry.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 1","pages":"82-90"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666190408151826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37132435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant Growth Promoting and Stress Mitigating Abilities of Soil Born Microorganisms.","authors":"Shahid Ali,&nbsp;Linan Xie","doi":"10.2174/2212798410666190515115548","DOIUrl":"https://doi.org/10.2174/2212798410666190515115548","url":null,"abstract":"<p><p>Abiotic stresses affect the plant growth in different ways and at different developmental stages that reduce the crop yields. The increasing world population continually demands more crop yields; therefore it is important to use low-cost technologies against abiotic stresses to increase crop productivity. Soil microorganisms survive in the soil associated with plants in extreme condition. It was demonstrated that these beneficial microorganisms promote plant growth and development under various stresses. The soil microbes interact with the plant through rhizospheric or endophytic association and promote the plant growth through different processes such as nutrients mobilization, disease suppression, and hormone secretions. The microorganisms colonized in the rhizospheric region and imparted the abiotic stress tolerance by producing 1-aminocyclopropane-1- carboxylate (ACC) deaminase, antioxidant, and volatile compounds, inducing the accumulation of osmolytes, production of exopolysaccharide, upregulation or downregulation of stress genes, phytohormones and change the root morphology. A large number of these rhizosphere microorganisms are now patented. In the present review, an attempt was made to throw light on the mechanism of micro-organism that operates during abiotic stresses and promotes plant survival and productivity.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 2","pages":"96-104"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666190515115548","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37264910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Recent Patents (2016-2019) on Plant Food Supplements with Potential Application in the Treatment of Neurodegenerative and Metabolic Disorders.","authors":"Rosalba Leuci,&nbsp;Leonardo Brunetti,&nbsp;Antonio Laghezza,&nbsp;Paolo Tortorella,&nbsp;Fulvio Loiodice,&nbsp;Luca Piemontese","doi":"10.2174/2212798411666200313145824","DOIUrl":"https://doi.org/10.2174/2212798411666200313145824","url":null,"abstract":"<p><p>In the near future, it is expected that the prevalence of illnesses related to the increasing life expectancies and quality of life, such as neurodegenerative diseases and cardiovascular diseases related to metabolic disorders, will soar to unprecedented levels, leading to high socioeconomic costs. To address this rising threat, natural products are emerging as a novel strategy for the prevention and therapy of these ages- and lifestyle-related diseases, thanks to their high marketability and few side effects. In this patent review, we summarize selected patents for food supplements, functional and fortified foods, filed from 2016 to 2019, categorizing them based on the biological activity of their components.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 2","pages":"145-153"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37733938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ammoides verticillata</i> Essential Oil as Biocontrol Agent of Selected Fungi and Pest of Olive Tree.","authors":"Hanane Senouci,&nbsp;Nassira G Benyelles,&nbsp;Mohammed Ea Dib,&nbsp;Jean Costa,&nbsp;Alain Muselli","doi":"10.2174/2212798410666190619110251","DOIUrl":"https://doi.org/10.2174/2212798410666190619110251","url":null,"abstract":"<p><strong>Background: </strong>Ammoides verticillata Briq is an aromatic and medicinal herb that has been widely used in folk medicine for treatment of several types of pathologies such as respiratory problems, colds, fever, headache, gastric disorders and renal infections.</p><p><strong>Objective: </strong>Essential oils have been subjected of numerous works for their antimicrobial and insecticidal properties which have been valued by hundreds of publications and patents. The main objective of this work was to evaluate the chemical composition, and the in vitro and in vivo antifungal, and insecticidal properties of essential oil of Ammoides verticillata.</p><p><strong>Methods: </strong>In this work, the air-dried aerial parts of Ammoides verticillata were hydrodistilled in a Clevenger-type apparatus. The essential oil isolated was analyzed using gas chromatography (GC) and mass spectrometry (GC/MS). The in vitro antifungal activity of the essential oil was investigated against four plant fungi using radial growth technique. The effect of the essential oil on disease development of olive caused by Penicillium crustosum and Aspergillus niger in the in vivo conditions. The fumigation test of the essential oil was evaluated against L3 stages, pupas and adults of Bactrocera oleae known as the olive fly.</p><p><strong>Results: </strong>The essential oil of A. verticillata was characterized mainly by carvacrol (44.3%), limonene (19.3%) and p-cymene (19.2%). The result of in vitro antifungal activity of essential oil showed an interesting antifungal inhibition against Alternaria alternata and Fusarium solani strains with percentage inhibition of 89%. Furthermore, oil of A. verticillata has demonstrated promising in vivo antifungal activity to control infections of olives caused by Aspergillus niger and Penicillium crustosum. In addition, the essential oil exhibited fumigation toxicity against Bactrocera oleae with mortality percentage of 100% at 2 μL/L air.</p><p><strong>Conclusion: </strong>The results showed that essential oil of A. verticillata has interesting biological activities, which suggests that oil have the potential to be used as biocide to protect olives of pathogenic fungi and pests.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 2","pages":"182-188"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666190619110251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37342999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Sustainable Plant Growth and Differentiation and the Role of Hormones in Controlling Growth and Development of Plants Under Various Stresses.","authors":"Shahid Ali,&nbsp;Abdul Majeed Baloch","doi":"10.2174/2212798410666190619104712","DOIUrl":"https://doi.org/10.2174/2212798410666190619104712","url":null,"abstract":"<p><p>Plant development is different from animals by many fundamental aspects; as they have immobilized cells, a rigid cell wall, and the large central vacuole. Plant growth and cell division are restricted to the specific area of the shoot and root called meristems. Plants have the ability to carry out differentiation, dedifferentiation and redifferentiation. In plants, the growth and differentiation processes are controlled by hormonal and genetic factors. Phytohormones can exert independent/ dependent actions on plant growth and development. A pool of stem cells is placed at the niche of the apex meristem, which is the source of self-renewal of the cell system and its maintenance to provide cells to differentiated tissues. A complex interaction network between hormones and other factors maintains a balance between cell division and differentiation. Auxins promote the growth, gibberellins' function in seed germination, cytokinin's influence on cell division and delay leaf senescence; abscisic acid promotes the stomatal closure and bud dormancy, while salicylic acid promotes resistance against different diseases. Plants are often exposed to different abiotic and biotic stresses, for example, heat, cold, drought, salinity etc., whereas biotic stress arises mainly from fungi, bacteria, insect, etc. Phytohormones play a critical role in well-developed mechanisms that help to perceive the stress signal and enable the plant's optimal growth response. In this review, we studied both the intrinsic and extrinsic factors which govern growth and differentiation of plants under normal and stress condition. This review also deals with genetic modifications occurring in the cell and cell signaling during growth and differentiation.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 2","pages":"105-114"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666190619104712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37343000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Curcumin on Traumatic Brain Injury and Involved Molecular Signaling Pathways.","authors":"Tahereh Farkhondeh,&nbsp;Saeed Samarghandian,&nbsp;Babak Roshanravan,&nbsp;Leila Peivasteh-Roudsari","doi":"10.2174/2212798410666190617161523","DOIUrl":"https://doi.org/10.2174/2212798410666190617161523","url":null,"abstract":"<p><p>Traumatic Brain Injury (TBI) is one of the main causes of mortality and morbidity worldwide with no suitable treatment. The present study was designed to review the present literature about the protective effects of curcumin and the underlying mechanism against TBI. All published English language papers from beginning to 2019 were selected in this study. The findings indicate that curcumin may be effective against TBI outcomes by modulating the molecular signaling pathways involved in oxidative stress, inflammation, apoptosis, and autophagy. However, more experimental studies should be done to identify all mechanisms involved in the pathogenesis of TBI. Patents for Curcumin and chronic inflammation and traumatic brain injury management (WO2017097805A1 and US9101580B2) were published. In conclusion, the present study confirmed the potential therapeutic impact of curcumin for treating TBI.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 2","pages":"137-144"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798410666190617161523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37403940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocess Optimization for Exopolysaccharides Production by Ganoderma lucidum in Semi-industrial Scale.","authors":"Ramzy Abd Alsaheb,&nbsp;Koh Zwin Zjeh,&nbsp;Roslinda Abd Malek,&nbsp;Jaafar Kamil Abdullah,&nbsp;Ashraf El Baz,&nbsp;Nehal El Deeb,&nbsp;Daniel Dailin,&nbsp;Siti Zulaiha Hanapi,&nbsp;Dalia Sukmawati,&nbsp;Hesham El Enshasy","doi":"10.2174/2212798411666200316153148","DOIUrl":"https://doi.org/10.2174/2212798411666200316153148","url":null,"abstract":"<p><strong>Background: </strong>For many years, Ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained much attention based on their high biotherapeutic properties. Therefore, special attention has been paid during the last years for the production of mushrooms bioactive compounds in a closed cultivation system to shorten the cultivation time and increase the product yield.</p><p><strong>Objectives: </strong>This work focuses on the development of a simple cultivation strategy for exopolysaccharides (EPS) production using Ganoderma lucidum and submerged cultivation system.</p><p><strong>Methods: </strong>At first, the best medium supporting EPS production was chosen experimentally from the current published data. Second, like many EPS production processes, carbon and nitrogen concentrations were optimized to support the highest production of polysaccharides in the shake flask level. Furthermore, the process was scaled up in 16-L stirred tank bioreactor.</p><p><strong>Results: </strong>The results clearly demonstrated that the best cultivation strategy was cultivation under controlled pH conditions (pH 5.5). Under this condition, the maximal volumetric and specific yield of EPS production were, 5.0 g/L and 0.42 g/g, respectively.</p><p><strong>Conclusion: </strong>The current results clearly demonstrate the high potential use of submerged cultivation system as an alternative to conventional solid-state fermentation for EPS production by G. lucidum. Furthermore, the optimization of both carbon and nitrogen sources concentration and scaling up of the process showed a significant increase in both volumetric and specific EPS production.</p>","PeriodicalId":21061,"journal":{"name":"Recent patents on food, nutrition & agriculture","volume":"11 3","pages":"211-218"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/2212798411666200316153148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37742326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}