Regenerative Biomaterials最新文献

{"title":"Combined magnesium and silicon ions synergistically promote functional regeneration of skeletal muscle by regulating satellite cell fate.","authors":"Hangbin Xia, Chen Yang, Huili Li, Lingwei Huang, Zhen Zeng, Runrun Chi, Ziwei Yang, Yuzen Wang, Jiang Chang, Yiren Jiao, Wenzhong Li","doi":"10.1093/rb/rbaf008","DOIUrl":"10.1093/rb/rbaf008","url":null,"abstract":"<p><p>Muscle satellite cells (MuSCs) play a vital role in skeletal muscle regeneration. However, in intractable muscle diseases such as volumetric muscle loss (VML), the quantity and function of MuSCs are significantly reduced, severely limiting the body's inherent muscle regeneration capability. In this study, we propose a novel strategy to modulate the fate of MuSCs using a combination of bioactive magnesium (Mg) and silicon (Si) ions, sustainably delivered through magnesium silicate (MgSiO₃, MS) bioceramic-based scaffolds. <i>In vitro</i>, Mg and Si ions synergistically promote the proliferation and differentiation of MuSCs. Similarly, Mg and Si ions derived from MS/poly(L-lactic acid) (MS/PLLA) composite scaffold also increase the proliferation and differentiation ability of MuSCs. Furthermore, MS/PLLA composite scaffolds facilitate the activation of MuSCs, regeneration of muscle fiber and neovascularization, while inhibiting fibrosis, thereby effectively restoring muscle function and promoting tibialis anterior muscle functional regeneration in a VML mouse model. Mechanistically, the combination of Mg and Si ions promotes the activation and proliferation of MuSCs by activating the Notch1-Hes1 pathway. Besides, the combination of Mg and Si ions also improves the differentiation of MuSCs by up-regulating Myod and Myog, and enhances fusion by up-regulating Mymk and Mymx expression. The outcomes of our research introduce a promising approach to the treatment of skeletal muscle injuries and related diseases.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf008"},"PeriodicalIF":5.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal regulation of the bone immune microenvironment via a 'Zn²⁺-quercetin' hierarchical delivery system for bone regeneration.","authors":"Hengliang Sun, Yedan Chen, Xiaoqin Sang, Qingxiang Liu, Haoran Yu, Shaojun Hu, Yingji Mao, Li Zhang","doi":"10.1093/rb/rbaf006","DOIUrl":"10.1093/rb/rbaf006","url":null,"abstract":"<p><p>The immunoregulation of tissue-engineered bone has emerged as a prominent area for bone defect repair. While this field demonstrates considerable potential, effectively managing relevant factors and maintaining a balanced immune microenvironment in practical applications remain substantial challenges that require resolution. In this study, we tested a novel comprehensive hierarchical delivery system based on the requirements of a natural immune microenvironment for inflammatory factors, to optimize local immune responses through precise regulation of drug release. Quercetin (Que)-loaded zeolite imidazolate framework-8 (ZIF-8) nanoparticles were embedded in gelatin methacrylate to create a drug-release system featuring a Zn²⁺ shell and quercetin core. <i>In vivo</i> and <i>in vitro</i> studies demonstrated that this dual sustained-release hydrogel-ZIF-8 system can produce low concentrations of Zn²⁺ at an early stage, resulting in a mild anti-inflammatory effect and proliferation of bone marrow mesenchymal stem cells. Moreover, as inflammation advances, the release of quercetin works synergistically with Zn²⁺ to enhance anti-inflammatory responses, reconfigure the local microenvironment, and mitigate the inflammatory response that adversely impacts bone health by inhibiting the Nuclear Factor-kappa B (NF-κB) signaling pathway, thereby promoting osteogenic differentiation. This system is pioneering for sequential microenvironment regulation based on its diverse anti-inflammatory properties, offering a novel and comprehensive strategy for bone immune regulation in the clinical treatment of bone defects.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf006"},"PeriodicalIF":5.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing β-TCP with E-rhBMP-2-infused fibrin for vertical bone regeneration in a mouse calvarium model.","authors":"Kun Zhao, Mitsuaki Ono, Xindi Mu, Ziyi Wang, Shichao Xie, Tomoko Yonezawa, Masahiro Okada, Takuya Matsumoto, Takuo Kuboki, Toshitaka Oohashi","doi":"10.1093/rb/rbae144","DOIUrl":"10.1093/rb/rbae144","url":null,"abstract":"<p><p>Effective reconstruction of large bone defects, particularly in thickness, remains one of the major challenges in orthopedic and dental fields. We previously produced an <i>Escherichia coli</i>-based industrial-scale GMP-grade recombinant human bone morphogenetic protein-2 (E-rhBMP-2) and showed that the combination of E-rhBMP-2 with beta-tricalcium phosphate (β-TCP/E-rhBMP-2) can effectively promote bone reconstruction. However, the limited mechanical strength and poor morphology retention of β-TCP granules are key points that need optimization to obtain more effective grafts and further expand its clinical applications. Therefore, we combined β-TCP/E-rhBMP-2 with fibrin gel to enhance its mechanical properties and usability for vertical bone regeneration. We investigated the mechanical properties and vertical bone regeneration effects of the materials applied, with or without fibrin containing E-rhBMP-2, in a calvarial defect model in mice. Compression tests were conducted to assess the initial stability of the materials. Scanning electron microscopy and Fourier transform infrared spectroscopy were conducted to characterize the presence of fibrin on the scaffold. After 4 and 12 weeks of implantation, micro-computed tomography and histological and immunofluorescent analyses were performed to assess the morphology and volume of the newly formed bone. The fibrin-containing groups had significantly higher initial mechanical strength and higher ability to maintain their morphology <i>in vivo</i> compared to the counterparts without fibrin. However, fibrin gel alone suppressed the bone formation ability of β-TCP/E-rhBMP-2 whereas the presence of high doses of E-rhBMP-2 in fibrin gel resulted in material resorption and enhanced new bone formation. In conclusion, fibrin gel significantly improved the mechanical strength and surgical manageability of the β-TCP/E-rhBMP-2 scaffold, and the addition of E-rhBMP-2 to the fibrin gel further enhanced the vertical bone regeneration and initial structural integrity of the scaffold.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbae144"},"PeriodicalIF":5.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up IGF-I via high-toughness adaptive hydrogels for remodeling growth plate of children.","authors":"Zhiqiang Zhang, Haodong Li, Manning Qian, Yiming Zheng, Luhan Bao, Wenguo Cui, Dahui Wang","doi":"10.1093/rb/rbaf004","DOIUrl":"10.1093/rb/rbaf004","url":null,"abstract":"<p><p>The growth plate is crucial for skeletal growth in children, but research on repairing growth plate damage and restoring growth is limited. Here, a high-toughness adaptive dual-crosslinked hydrogel is designed to mimic the growth plate's structure, supporting regeneration and bone growth. Composed of aldehyde-modified bacterial cellulose (DBNC), methacrylated gelatin (GelMA) and sodium alginate (Alg), the hydrogel is engineered through ionic bonding and Schiff base reactions, creating a macroporous structure. This structure can transform into a denser form by binding with calcium ions. <i>In vitro</i>, the loose macroporous structure of the hydrogels can promote chondrogenic differentiation, and when it forms a dense structure by binding with calcium ions, it also can activate relevant chondrogenic signaling pathways under the influence of insulin-like growth factor I (IGF-1), further inhibiting osteogenesis. <i>In vivo</i> experiments in a rat model of growth plate injury demonstrated that the hydrogel promoted growth plate cartilage regeneration and minimized bone bridge formation by creating a hypoxic microenvironment that activates IGF-1-related pathways. This environment encourages chondrogenic differentiation while preventing the undesired formation of bone tissue within the growth plate area. Overall, the dual-crosslinked hydrogel not only mimics the growth plate's structure but also facilitates localized IGF-1 expression, effectively reshaping the growth plate's function. This approach represents a promising therapeutic strategy for treating growth plate injuries, potentially addressing challenges associated with skeletal growth restoration in pediatric patients.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf004"},"PeriodicalIF":5.6,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing sterilization and functional properties in Poloxamer 407 hydrogels: comparing heat and radiation techniques.","authors":"Angela De Lauretis, Anne Eriksson Agger, Antara Pal, Jan Skov Pedersen, Szymon Mikolaj Szostak, Reidar Lund, Ståle Petter Lyngstadaas, Jan Eirik Ellingsen, Dirk Linke, Håvard Jostein Haugen","doi":"10.1093/rb/rbaf005","DOIUrl":"10.1093/rb/rbaf005","url":null,"abstract":"<p><p>Poloxamer 407, also known as Pluronic^® F127, is gaining interest in the cosmetic, biomedical and pharmaceutical fields for its biocompatibility, safety and thermo-sensitive properties. Ensuring sterility is critical in clinical applications, and sterilization is often preferred over aseptic processing. However, sterilization can impact the functional properties of the hydrogel. In this study, we investigate the effects of steam heat (121°C, 20 min), dry heat (160°C, 1 h), gamma irradiation (25 kGy) and electron beam (e-beam) irradiation (15 and 25 kGy) on a 30% w/v Poloxamer 407 hydrogel formulation. Our analysis encompasses gelling properties, pH, Fourier-transform infrared spectroscopy, gel permeation chromatography, small-angle X-ray scattering, rheology, swelling, degradation by-products and lactate dehydrogenase release of the sterilized hydrogels, comparing them to a non-sterile counterpart. We demonstrated that heat sterilization alters the hydrogel's gelling and structural properties due to water evaporation and oxidation under harsh temperature conditions, especially when applying the dry heat method. Gamma irradiation proved unsuitable, resulting in an acidic and cytotoxic hydrogel due to oxidative degradation. In contrast, e-beam irradiation preserves the hydrogel's elasticity, gelling and structural properties while enhancing mechanical resilience and moderating swelling. Therefore, e-beam irradiation within the 15-25 kGy range appears to be the most suitable method for sterilizing a 30% w/v Poloxamer 407 hydrogel.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf005"},"PeriodicalIF":5.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocytosis-mediated healing: recombinant human collagen type III chain-induced wound healing for scar-free recovery.","authors":"Jian Jin, Haihang Li, Zhengli Chen, Qingsong Liu, Jiqiu Chen, Zihan Tao, Xudong Hong, Yinjia Ding, Yue Zhou, Aifen Chen, Xudong Zhang, Kaiyang Lv, Liangliang Zhu, Shihui Zhu","doi":"10.1093/rb/rbae149","DOIUrl":"10.1093/rb/rbae149","url":null,"abstract":"<p><p>Scar formation can be effectively prevented when the proportion of collagen type I (Col I)/type III (Col III) is reduced. Unlike Col III, recombinant human collagen type III chain (RHC III chain) does not possess a triple helical structure. This study aimed to elucidate the capacity of fibroblasts to uptake RHC III chain, reduce the Col I/Col III ratio and determine its effects on wound healing and scar. RHC III chain demonstrates qualified cell compatibility. In cell experiments, immunofluorescence and western blot (WB) analyses revealed an increase in the polyhistidine tag level, indicating that RHC III chain in internalized by these cells. Transmission electron microscopy showed increased intracellular phagocytic activity, indicating that RHC III chain enters fibroblasts by endocytosis. The immunofluorescence and WB showed that Col III synthesis enhanced, and Col I/Col III ratio reduced. However, the polyhistidine tag disappeared with time, indicating that RHC III chain degraded within cells and then synthesized into Col III. The content of newly synthesized Col III increases, but real-time fluorescence quantitative showed a decrease in Col III related gene content suggests the formation of negative feedback. However, due to the sufficient raw materials, the amount of Col III synthesis is still increasing, leading to the reduction of the ratio of type I collagen/type III collagen, which beneficial to wound healing and reduce scar hyperplasia. In animal experiments, the SD rat full-thickness skin defect model of wound suggests that RHC III chain also takes effect through endocytosis and ultimately promotes wound healing. The rabbit ear scar model suggests that RHC III chain inhibits scar proliferation by reducing the ratio of Col I/Col III. In summary, RHC III chain was endocytosed by fibroblasts to promote native Col III synthesis, as well as promote wound healing and reduce scar hyperplasia.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbae149"},"PeriodicalIF":5.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria-targeting nanomedicines with autophagy inhibitor to enhance cancer photothermal-chemotherapy.","authors":"Shuqi Chen, Wenxia Gao, Shuhua Chang, Bin He, Congbo Zhang, Miaochang Liu, Xueting Ye","doi":"10.1093/rb/rbae141","DOIUrl":"10.1093/rb/rbae141","url":null,"abstract":"<p><p>In this article, we fabricated nanomedicines with mitochondrial targeting function and autophagy inhibitor for enhancing cancer photothermal-chemotherapy. The nanoparticles were fabricated with gold nanoparticles (AuNPs) as cores and amphiphilic dextran with (3-carboxypropyl) triphenyl phosphorus bromide and β-cyclodextrin (β-CD) modification (TPP-DCD) as shells; the chemotherapeutic doxorubicin (DOX) and autophagy inhibitor chloroquine (CQ) were encapsulated in the nanoparticles. The TPP-DCD was synthesized via the immobilization of 2-aminoethanethiol modified β-CD and (3-carboxypropyl) triphenylphosphonium bromide on dextran to receive coordination interaction with AuNPs and mitochondria targeting. The size, morphology and properties of the Au@DOX/CQ@TPP-DCD nanoparticles were studied. The nanomedicines efficiently targeted cellular mitochondria to produce reactive oxygen species and photothermal effect under NIR irradiation. The released DOX and CQ could not only kill tumor cells directly, but also inhibit the autophagy of cancer cells to enhance therapeutic effects. Both <i>in vitro</i> and <i>in vivo</i> anticancer activities of the nanomedicines were investigated in detail. The <i>in vivo</i> imaging demonstrated that the Au@DOX/CQ@TPP-DCD nanomedicines exhibited efficient targeting, accumulation and retention in tumor-bearing mice. The apoptosis of cancer cells and tumor suppression were greatly accelerated with the addition of 808 nm NIR irradiation. The Au@DOX/CQ@TPP-DCD nanomedicine exhibited significant synergistic therapy, as 75% of tumors in mice disappeared. The Au@DOX/CQ@TPP-DCD nanoparticle is a promising nanomedicine for cancer therapy with synergistic effects.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbae141"},"PeriodicalIF":5.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermally controlled ICG@ZIF-8/PLGA coating to modify the degradation behavior and biocompatibility of Zn-Li alloy for bone implants.","authors":"Ting Zhang, Yameng Yu, Wei Yuan, Zeqi Ren, Yan Cheng, Shuilin Wu, Yufeng Zheng, Dandan Xia","doi":"10.1093/rb/rbaf001","DOIUrl":"10.1093/rb/rbaf001","url":null,"abstract":"<p><p>Biodegradable Zn alloy has recently gained attention for use in bone implants considering its biodegradability, attractive mechanical properties and bioactivity. However, excessive corrosion of Zn alloy at the early stage of implantation may cause severe cytotoxicity, resulting in insufficient osseointegration, which hinders the clinical use of Zn alloy. In this study, we designed a photothermally controlled degradative hybrid coating as a corrosion-protective barrier with the intention of preventing Zn ion burst release during the early stages of implantation and regaining the alloy's corrosion advantage later on. The coating consists of zeolite imidazole skeleton-encapsulated indocyanine green core-shell-structured nanoparticles and polylactic coglycolic acid (ICG@ZIF-8/PLGA) on pristine Zn-0.8 (wt.%) Li (ZL) alloy. The electrochemical test results indicated that coating ZL with ICG@ZIF-8/PLGA can effectively reduce its corrosion current density (i_corr) from 2.48 × 10^-5 A·cm^-2 to 2.10 × 10^-8 A·cm^-2. After near-infrared (NIR) light irradiation, ICG@ZIF-8 heated PLGA coating to reach Tg, causing the coating to degrade and the i_corr of the coated ZL alloy decreased to 2.50 × 10^-7 A·cm^-2, thus restoring corrosion advantage. Both <i>in vitro</i> and <i>in vivo</i> investigations showed that the coated ZL alloy had acceptable biocompatibility. Overall, the developed photothermally controlled coating improved the Zn alloy's resistance to corrosion and allowed for the adjustment of the Zn alloy's degradation rate through 808-nm NIR light irradiation.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf001"},"PeriodicalIF":5.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Material surface conjugated with fibroblast growth factor-2 for pluripotent stem cell culture and differentiation.","authors":"Tzu-Cheng Sung, Zhi-Xian Pan, Ting Wang, Hui-Yu Lin, Chia-Lun Chang, Ling-Chun Hung, Suresh Kumar Subbiah, Remya Rajan Renuka, Shih-Jie Chou, Shih-Hwa Chiou, Idaszek Joanna, Henry Hsin-Chung Lee, Gwo-Jang Wu, Akon Higuchi","doi":"10.1093/rb/rbaf003","DOIUrl":"10.1093/rb/rbaf003","url":null,"abstract":"<p><p>Fibroblast growth factor-2 (FGF-2) is a critical molecule for sustaining the pluripotency of human pluripotent stem (PS) cells. However, FGF-2 is extremely unstable and cannot be stored long periods at room temperature. Therefore, the following FGF-2-conjugated cell culture materials were developed to stabilize FGF-2: FGF-2-conjugated polyvinyl alcohol (PVAI-C-FGF) hydrogels and FGF-2-conjugated carboxymethyl cellulose-coated (CMC-C-FGF) dishes. Human induced pluripotent stem (iPS) cells were proliferated on recombinant vitronectin (rVN)-coated PVAI-C-FGF hydrogels and CMC-C-FGF dishes in medium without FGF-2. Human iPS cells could not be cultivated on rVN-coated PVAI-C-FGF hydrogels for more than two passages but could proliferate on rVN-coated CMC-C-FGF dishes. These results indicated that the amount of immobilized FGF-2 and the base cell materials are important, including the amount of immobilized rVN and the conformation of FGF-2 on the surfaces. When human iPS cells were proliferated on rVN-coated CMC-C-FGF surfaces in medium containing no FGF-2 for 10 passages, their pluripotency and potential to differentiate into cells originating from three germ layers were maintained <i>in vivo</i> and <i>in vitro</i>. Furthermore, the cells could extensively differentiate into cardiomyocytes, which can be used for cardiac infarction treatment in future and retinal pigment epithelium for retinal pigmentosa treatment in future. The FGF-2-immobilized surface could enable human PS cell culture in medium that does not need to contain unstable FGF-2. The amount of FGF-2 immobilization on the rVN-coated CMC-C-5FGF and CMC-C-20FGF dishes was reduced to 93.6 and 52.2 times, respectively, which is less than the conventional amount of FGF-2 used in culture medium for one passage (6 days) of human iPS cell culture. This reduction resulted from the stabilization of unstable FGF-2 by the immobilization of FGF-2, which was achieved by utilizing optimal base materials (CMC), coating materials (rVN) and long-joint segment (PEG4-SPDP) design.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf003"},"PeriodicalIF":5.6,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-responsive hydrogel with dual-crosslinked network of polyvinyl alcohol/boric acid for controlled release of salvianolic acid B: novel pro-regenerative mechanisms in scar inhibition and wound healing.","authors":"Wei Song, Chao Zhang, Zhao Li, Kejia Li, Yi Kong, Jinpeng Du, Yue Kong, Xu Guo, Xiaoyan Ju, Meng Zhu, Ye Tian, Sha Huang, Zhongwei Niu","doi":"10.1093/rb/rbaf002","DOIUrl":"10.1093/rb/rbaf002","url":null,"abstract":"<p><p>This study investigates a novel pH-responsive hydrogel composed of polyvinyl alcohol (PVA) and boric acid (BA) designed for the controlled release of salvianolic acid B (SAB), addressing the critical challenge of scar formation and skin regeneration. The dual-crosslinked network architecture of the hydrogel exhibits remarkable pH sensitivity, enabling it to achieve a peak SAB release within 48 hours in the acidic microenvironment characteristic of early-stage wound healing. <i>In vitro</i> assessments demonstrated that the PVA-BA-SAB hydrogel significantly inhibits fibroblast activation and mitigates abnormal collagen deposition, effectively preventing excessive scar formation. Transcriptome sequencing reveals the potential role of PVA-BA-SAB hydrogel in balancing TGF-β and Wnt signaling pathways. Furthermore, <i>in vivo</i> studies revealed enhanced tissue regeneration, characterized by improved collagen organization and increased vascularization, as well as the promotion of mature hair follicle development. The hydrogel's biocompatibility, mechanical robustness and adhesive properties were also thoroughly evaluated, confirming its suitability for clinical applications. These findings suggest that the PVA-BA-SAB hydrogel fully exerts the excellent characteristics of biomaterials and maximizes the pharmacological effect of SAB. Our innovative drug delivery system not only facilitates enhanced wound healing but also offers a strategic approach to minimize scarring. This research provides valuable insights into innovative therapeutic strategies for effective wound management and tissue repair.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"12 ","pages":"rbaf002"},"PeriodicalIF":5.6,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}