Regenerative Biomaterials最新文献_第9页

Mild Synthesis of Ultra-Bright Carbon Dots with Solvatochromism for Rapid Lipid Droplet Monitoring in Varied Physiological Process 温和合成具有溶解变色功能的超亮碳点，用于在各种生理过程中快速监测脂滴

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2024-01-17 DOI: 10.1093/rb/rbad109

Borui Su, Dong Gao, Nini Xin, Kai Wu, Mei Yang, Shichao Jiang, Yusheng Zhang, Jie Ding, Chengheng Wu, Jing Sun, Dan Wei, Hongsong Fan, Zhenzhen Guo

{"title":"Mild Synthesis of Ultra-Bright Carbon Dots with Solvatochromism for Rapid Lipid Droplet Monitoring in Varied Physiological Process","authors":"Borui Su, Dong Gao, Nini Xin, Kai Wu, Mei Yang, Shichao Jiang, Yusheng Zhang, Jie Ding, Chengheng Wu, Jing Sun, Dan Wei, Hongsong Fan, Zhenzhen Guo","doi":"10.1093/rb/rbad109","DOIUrl":"https://doi.org/10.1093/rb/rbad109","url":null,"abstract":"Lipid droplets (LDs) participating in various cellular activities and are increasingly being emphasized. Fluorescence imaging Provides powerful tool for dynamic tracking of LDs, however, most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield (PLQY), poor photostability and tedious washing procedures. Herein, a novel yellow-emissive carbon dot (OT-CD) has been synthesized conveniently with high PLQY up to 90%. Besides, OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2, which is much higher than most LDs probes. These characters enable OT-CD high brightness, stable and wash-free LDs probing, and feasible for in vivo imaging. Then, detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded, including ferroptosis and other diseases processes. Furthermore, fast whole imaging of zebrafish and identified LD enrichment in injured liver indicate its further feasibility for in vivo application. In contrast to the reported studies to date, this approach provides a versatile conventional synthesis system for high performance LDs targeting probes, combing the advantages of easy and high yield production, as well as robust brightness and stability for long-term imaging, facilitating investigations into organelle interactions and LD-associated diseases.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"50 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139498117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

dECM restores macrophage immune homeostasis and alleviates iron overload to promote DTPI healing. dECM 可恢复巨噬细胞的免疫平衡，减轻铁超载，从而促进 DTPI 愈合。

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2024-01-17 eCollection Date: 2024-01-01 DOI: 10.1093/rb/rbad118

Ju Zhang, Ruijuan Si, Yu Gao, Hui Shan, Qi Su, Zujian Feng, Pingsheng Huang, Deling Kong, Weiwei Wang

{"title":"dECM restores macrophage immune homeostasis and alleviates iron overload to promote DTPI healing.","authors":"Ju Zhang, Ruijuan Si, Yu Gao, Hui Shan, Qi Su, Zujian Feng, Pingsheng Huang, Deling Kong, Weiwei Wang","doi":"10.1093/rb/rbad118","DOIUrl":"https://doi.org/10.1093/rb/rbad118","url":null,"abstract":"Due to its highly insidious and rapid progression, deep tissue pressure injury (DTPI) is a clinical challenge. Our previous study found that DTPI may be a skeletal muscle injury dominated by macrophage immune dysfunction due to excessive iron accumulation. Decellularized extracellular matrix (dECM) hydrogel promotes skeletal muscle injury repair. However, its role in polarizing macrophages and regulating iron metabolism in DTPI remains unclear. Here, porcine dECM hydrogel was prepared, and its therapeutic function and mechanism in repairing DTPI were investigated. The stimulus of dECM hydrogel toward RAW264.7 cells resulted in a significantly higher percentage of CD206+ macrophages and notably decreased intracellular divalent iron levels. In mice DTPI model, dECM hydrogel treatment promoted M1 to M2 macrophage conversion, improved iron metabolism and reduced oxidative stress in the early stage of DTPI. In the remodeling phase, the dECM hydrogel remarkably enhanced revascularization and accelerated skeletal muscle repair. Furthermore, the immunomodulation of dECM hydrogels in vivo was mainly involved in the P13k/Akt signaling pathway, as revealed by GO and KEGG pathway analysis, which may ameliorate the iron deposition and promote the healing of DTPI. Our findings indicate that dECM hydrogel is promising in skeletal muscle repair, inflammation resolution and tissue injury healing by effectively restoring macrophage immune homeostasis and normalizing iron metabolism.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"11 ","pages":"rbad118"},"PeriodicalIF":6.7,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10884736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fe-Zn alloy, a new biodegradable material capable of reducing ROS and inhibiting oxidative stress 能减少 ROS 和抑制氧化应激的新型可生物降解材料--铁锌合金

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2024-01-13 DOI: 10.1093/rb/rbae002

Shuaikang Yang, Weiqiang Wang, Yanan Xu, Yonghui Yuan, Shengzhi Hao

{"title":"Fe-Zn alloy, a new biodegradable material capable of reducing ROS and inhibiting oxidative stress","authors":"Shuaikang Yang, Weiqiang Wang, Yanan Xu, Yonghui Yuan, Shengzhi Hao","doi":"10.1093/rb/rbae002","DOIUrl":"https://doi.org/10.1093/rb/rbae002","url":null,"abstract":"Fe-based biodegradable materials have attracted significant attention due to their exceptional mechanical properties and favorable biocompatibility. Currently, research on Fe-based materials mainly focuses on regulating the degradation rate. However, excessive release of Fe ions during material degradation will induce the generation of reactive oxygen species (ROS), leading to oxidative stress and ferroptosis. Therefore, the control of ROS release and the improvement of biocompatibility for Fe-based materials are very important. In this study, new Fe-Zn alloys were prepared by electrodeposition with the intention of using Zn as an antioxidant to reduce oxidative damage during alloy degradation. Initially, the impact of three potential degradation ions (Fe2+, Fe3+, Zn2+) from the Fe-Zn alloy on human endothelial cells’ (ECs) activity and migration ability was investigated. Subsequently, cell adhesion, cell activity, ROS production, and DNA damage were assessed at various locations surrounding the alloy. Finally, the influence of different concentrations of Zn2+ in the medium on cell viability and ROS production was evaluated. High levels of ROS exhibited evident toxic effects on ECs and promoted DNA damage. As an antioxidant, Zn2+ effectively reduced ROS production around Fe and improved the cell viability on its surface at a concentration of 0.04 mmol/L. These findings demonstrate that Fe-Zn alloy can attenuate the ROS generated from Fe degradation thereby enhancing cytocompatibility.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"17 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139459856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun PCL/gelatin/arbutin nanofiber membranes as potent reactive oxygen species scavengers to accelerate cutaneous wound healing 电纺 PCL/明胶/熊果苷纳米纤维膜作为强效活性氧清除剂，可加速皮肤伤口愈合

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2024-01-13 DOI: 10.1093/rb/rbad114

Mindong Du, Shuhan Liu, Nihan Lan, Ruiming Liang, Shengde Liang, Maoqiang Lan, Disen Feng, Li Zheng, Qingjun Wei, Ke Ma

{"title":"Electrospun PCL/gelatin/arbutin nanofiber membranes as potent reactive oxygen species scavengers to accelerate cutaneous wound healing","authors":"Mindong Du, Shuhan Liu, Nihan Lan, Ruiming Liang, Shengde Liang, Maoqiang Lan, Disen Feng, Li Zheng, Qingjun Wei, Ke Ma","doi":"10.1093/rb/rbad114","DOIUrl":"https://doi.org/10.1093/rb/rbad114","url":null,"abstract":"The presence of excessive reactive oxygen species (ROS) at a skin wound site is an important factor affecting wound healing. ROS scavenging, which regulates the ROS microenvironment, is essential for wound healing. In this study, we used novel electrospun PCL/gelatin/arbutin(PCL/G/A) nanofibrous membranes as wound dressings, with PCL/gelatin(PCL/G) as the backbone, and plant-derived arbutin (hydroquinone-β-D-glucopyranoside, ARB) as an effective antioxidant that scavenges ROS and inhibits bacterial infection in wounds. The loading of ARB increased the mechanical strength of the nanofibres, with a water vapour transmission rate of more than 2500 g/(m2×24 h), and the water contact angle decreased, indicating that hydrophilicity and air permeability were significantly improved. Drug release and degradation experiments showed that the nanofibre membrane controlled the drug release and exhibited favourable degradability. Haemolysis experiments showed that the PCL/G/A nanofibre membranes were biocompatible, and DPPH and ABTS+ radical scavenging experiments indicated that PCL/G/A could effectively scavenge ROS to reflect the antioxidant activity. In addition, haemostasis experiments showed that PCL/G/A had good haemostatic effects in vitro and in vivo. In vivo animal wound closure and histological staining experiments demonstrated that PCL/G/A increased collagen deposition and remodelled epithelial tissue regeneration while showing good in vivo biocompatibility and non-toxicity. In conclusion, we successfully prepared a multifunctional wound dressing, PCL/G/A, for skin wound healing and investigated its potential role in wound healing, which is beneficial for the clinical translational application of phytomedicines.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"270 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139459960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing cytokine-induced killer cells to accelerate diabetic wound healing: an approach to regulating post-traumatic inflammation. 利用细胞因子诱导的杀伤细胞加速糖尿病伤口愈合：一种调节创伤后炎症的方法。

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2024-01-09 eCollection Date: 2024-01-01 DOI: 10.1093/rb/rbad116

Yixi Yang, Cheng Zhang, Yuan Jiang, Yijun He, Jiawei Cai, Lin Liang, Zhaohuan Chen, Sicheng Pan, Chu Hua, Keke Wu, Le Wang, Zhiyong Zhang

{"title":"Harnessing cytokine-induced killer cells to accelerate diabetic wound healing: an approach to regulating post-traumatic inflammation.","authors":"Yixi Yang, Cheng Zhang, Yuan Jiang, Yijun He, Jiawei Cai, Lin Liang, Zhaohuan Chen, Sicheng Pan, Chu Hua, Keke Wu, Le Wang, Zhiyong Zhang","doi":"10.1093/rb/rbad116","DOIUrl":"10.1093/rb/rbad116","url":null,"abstract":"Impaired immunohomeostasis in diabetic wounds prolongs inflammation and cytokine dysfunction, thus, delaying or preventing wound-surface healing. Extensive clinical studies have been conducted on cytokine-induced killer (CIK) cells recently, as they can be easily proliferated using a straightforward, inexpensive protocol. Therefore, the function of CIK cells in regulating inflammatory environments has been drawing attention for clinical management. Throughout the current investigation, we discovered the regenerative capacity of these cells in the challenging environment of wounds that heal poorly due to diabetes. We demonstrated that the intravenous injection of CIK cells can re-establish a proregenerative inflammatory microenvironment, promote vascularization and, ultimately, accelerate skin healing in diabetic mice. The results indicated that CIK cell treatment affects macrophage polarization and restores the function of regenerative cells under hyperglycemic conditions. This novel cellular therapy offers a promising intervention for clinical applications through specific inflammatory regulation functions.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"11 ","pages":"rbad116"},"PeriodicalIF":6.7,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10850840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of millimeter-scale vascularized engineered myocardial tissue using a mixed gel 利用混合凝胶构建毫米级血管化工程心肌组织

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2023-12-28 DOI: 10.1093/rb/rbad117

Ming Ke, Wenhui Xu, Yansha Hao, Feiyang Zheng, Guanyuan Yang, Yonghong Fan, Fangfang Wang, Zhiqiang Nie, Chuhong Zhu

{"title":"Construction of millimeter-scale vascularized engineered myocardial tissue using a mixed gel","authors":"Ming Ke, Wenhui Xu, Yansha Hao, Feiyang Zheng, Guanyuan Yang, Yonghong Fan, Fangfang Wang, Zhiqiang Nie, Chuhong Zhu","doi":"10.1093/rb/rbad117","DOIUrl":"https://doi.org/10.1093/rb/rbad117","url":null,"abstract":"Engineering myocardium has shown great clinal potential for repairing permanent myocardial injury. However, the lack of perfusing blood vessels and difficulties in preparing a thick engineered myocardium result in its limited clinical use. We prepared a mixed gel containing fibrin (5 mg/mL) and collagen I (0.2 mg/mL), and verified that human umbilical vein endothelial cells (HUVECs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) could form microvascular lumens and myocardial cell clusters by harnessing the low-hardness and hyperelastic characteristics of fibrin. hiPSC-CMs and HUVECs in the mixed gel formed self-organized cell clusters, which were then cultured in different media using a three-phase approach. The successfully constructed vascularized engineered myocardial tissue had a spherical structure and final diameter of 1–2 mm. The tissue exhibited autonomous beats that occurred at a frequency similar to a normal human heart rate. The internal microvascular lumen could be maintained for 6 weeks and showed good results during preliminary surface re-vascularization in vitro and vascular remodeling in vivo. In summary, we propose a simple method for constructing vascularized engineered myocardial tissue, through phased cultivation that does not rely on high-end manufacturing equipment and cutting-edge preparation techniques. The constructed tissue has potential value for clinical use after preliminary evaluation.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"43 5 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139063266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immediate implantation of ultrafine fiber slow-release system based on cell electrospinning to induce osteogenesis of mesenchymal stem cells 基于细胞电纺丝的超细纤维缓释系统即时植入，诱导间充质干细胞成骨

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2023-12-24 DOI: 10.1093/rb/rbad113

Tao Lu, Long Yang, Zhuo-yang Li, Yin Liu, Shun-en Xu, Chuan Ye

{"title":"Immediate implantation of ultrafine fiber slow-release system based on cell electrospinning to induce osteogenesis of mesenchymal stem cells","authors":"Tao Lu, Long Yang, Zhuo-yang Li, Yin Liu, Shun-en Xu, Chuan Ye","doi":"10.1093/rb/rbad113","DOIUrl":"https://doi.org/10.1093/rb/rbad113","url":null,"abstract":"This study presents the development and evaluation of a P34HB ultrafine fiber slow-release system for in vivo osteogenic induction of Human Umbilical Cord Mesenchymal Stem Cells (HUCMSCs). Utilizing dual-nozzle and cell electrospinning techniques, the system encapsulates L-ascorbic acid-2-phosphate magnesium (ASP), β-glycerophosphate sodium (GP), and dexamethasone (DEX) within the fibers, ensuring sustained osteogenic differentiation. The scaffold's morphology, characterization, hydrophilicity, mechanical properties, and cellular behavior were examined. Immediate subcutaneous implantation in rabbits was conducted to observe its ectopic osteogenic induction effect. Successfully fabricated P34HB ultrafine fiber slow-release system. Characterization confirmed the uniform distribution of HUCMSCs and inducing components within the scaffold, with no chemical reactions affecting the active components. In vitro tests showcased a prolonged release of DEX and ASP, while biocompatibility assays highlighted the scaffold's suitability for cellular growth. Alizarin Red, Type I Collagen, and Osteopontin (OPN) staining verified the scaffold's potent osteogenic induction effect on HUCMSCs. Notably, immediate implantation into New Zealand white rabbits led to significant new bone formation within 8 weeks. These findings underscore the system's potential for immediate in vivo implantation without prior in vitro induction, marking a promising advancement in bone tissue engineering.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"98 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139053853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in functional metal organic frameworks for bio-medical application 用于生物医学的功能性金属有机框架的最新进展

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2023-12-23 DOI: 10.1093/rb/rbad115

Wenwen Chai, Xiaochen Chen, Jing Liu, Liyan Zhang, Chunyu Liu, Li Li, Honiball John Robert, Haobo Pan, Xu Cui, Deping Wang

{"title":"Recent progress in functional metal organic frameworks for bio-medical application","authors":"Wenwen Chai, Xiaochen Chen, Jing Liu, Liyan Zhang, Chunyu Liu, Li Li, Honiball John Robert, Haobo Pan, Xu Cui, Deping Wang","doi":"10.1093/rb/rbad115","DOIUrl":"https://doi.org/10.1093/rb/rbad115","url":null,"abstract":"Metal-organic frameworks (MOFs) have a high specific surface area, adjustable pores and can be used to obtain functional porous materials with diverse and well-ordered structures through coordination and self-assembly, which has intrigued wide interest in a broad range of disciplines. In the arena of biomedical engineering, the functionalized modification of MOFs has produced drug carriers with excellent dispersion and functionalities such as target delivery and response release, with promising applications in bio-detection, disease therapy, tissue healing, and other areas. This review summarizes the present state of research on the functionalization of MOFs by physical binding or chemical cross-linking of small molecules, polymers, biomacromolecules, and hydrogels and evaluates the role and approach of MOFs functionalization in boosting the reactivity of materials. On this basis, research on the application of functionalized MOFs composites in biomedical engineering fields such as drug delivery, tissue repair, disease treatment, bio-detection and imaging is surveyed, and the development trend and application prospects of functionalized MOFs as an important new class of biomedical materials in the biomedical field are anticipated, which may provide some inspiration and reference for further development of MOF for bio-medical applications.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"20 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139054072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration 钛种植体上的纤维拓扑结构促进了 pBMP2 激活基质的骨结合

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2023-12-21 DOI: 10.1093/rb/rbad111

Ting He, Yichun Wang, Ruohan Wang, Huan Yang, Xueyi Hu, Yiyao Pu, Binbin Yang, Jingyuan Zhang, Juan Li, Chongxiang Huang, Rongrong Jin, Yu Nie, Xingdong Zhang

{"title":"Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration","authors":"Ting He, Yichun Wang, Ruohan Wang, Huan Yang, Xueyi Hu, Yiyao Pu, Binbin Yang, Jingyuan Zhang, Juan Li, Chongxiang Huang, Rongrong Jin, Yu Nie, Xingdong Zhang","doi":"10.1093/rb/rbad111","DOIUrl":"https://doi.org/10.1093/rb/rbad111","url":null,"abstract":"\u0000 Titanium (Ti) implants have been extensively used after surgical operations. Its surface bioactivity is of importance to facilitate integration with surrounding bone tissue, and ultimately ensure stability and long-term functionality of the implant. The plasmid DNA-activated matrix (DAM) coating on the surface could benefit osseointegration but is still trapped by poor transfection for further application, especially on the bone marrow mesenchymal stem cells (BMSCs) in vivo practical conditions. Herein, we constructed a DAM on the surface of fibrous-grained titanium (FG Ti) composed of phase-transition lysozyme (P) as adhesive, cationic arginine-rich lipid (RLS) as the transfection agent, and plasmid DNA (pDNA) for bone morphology protein 2 (BMP-2) expression. The cationic lipid RLS improved up to 30-fold higher transfection than that of commercial reagents (Lipofectamine 2000 and polyethyleneimine) on MSC. And importantly, Ti surface topology not only promotes the DAM to achieve high transfection efficiency (∼75.7% positive cells) on MSC due to the favorable combination but also reserves its contact induction effect for osteoblasts. Upon further exploration, the fibrous topology on FG Ti could boost pDNA uptake for gene transfection, and cell migration in MSC through cytoskeleton remodeling and induce contact guidance for enhanced osteointegration. At the same time, the cationic RLS together with adhesive P were both antibacterial, showing up to 90% inhibition rate against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) with reduced adherent microorganisms and disrupted bacteria. Finally, the FG Ti-P/pBMP2 implant achieved accelerated bone healing capacities through highly efficient gene delivery, aligned surface topological structure, and increased antimicrobial properties in a rat femoral condylar defect model.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"67 34","pages":""},"PeriodicalIF":6.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision cardiac targeting: empowering curcumin therapy through smart exosome-mediated drug delivery in myocardial infarction 精准的心脏靶向治疗：通过智能外泌体介导的心肌梗死药物输送增强姜黄素治疗的能力

IF 6.7 1区医学

Regenerative Biomaterials Pub Date : 2023-12-14 DOI: 10.1093/rb/rbad108

Ming Chen, Shengnan Wang, Yihuan Chen, Han Shen, Lei Chen, Liang Ding, Qingsong Tang, Ziying Yang, Weiqian Chen, Zhenya Shen

{"title":"Precision cardiac targeting: empowering curcumin therapy through smart exosome-mediated drug delivery in myocardial infarction","authors":"Ming Chen, Shengnan Wang, Yihuan Chen, Han Shen, Lei Chen, Liang Ding, Qingsong Tang, Ziying Yang, Weiqian Chen, Zhenya Shen","doi":"10.1093/rb/rbad108","DOIUrl":"https://doi.org/10.1093/rb/rbad108","url":null,"abstract":"Nanoparticle-mediated drug delivery has emerged as a highly promising and effective therapeutic approach for addressing myocardial infarction. However, clinical translation tends to be a failure due to low cardiac retention as well as liver and spleen entrapment in previous therapies. Herein, we report a two-step exosome delivery system, which precludes internalization by the mononuclear phagocyte system before the delivery of therapeutic cardiac targeting exosomes (ExoCTP). Importantly, curcumin released by ExoCTP diminishes reactive oxygen species over-accumulation in ischemic myocardium, as well as serum levels of lactate dehydrogenase, malonyldialdehyde, superoxide dismutase, and glutathione, indicating better antioxidant capacity than free curcumin. Finally, our strategy was proven to greatly potentiate the delivery and therapeutic efficacy of curcumin without systemic toxicity. Taken together, our smart exosome-mediated drug delivery strategy can serve either as therapeutics alone or in combination with other drugs for effective heart targeting and subsequent wound healing.","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":"4 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138686988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0