Renal Failure最新文献

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Construction and validation of a fall risk prediction model in elderly maintenance hemodialysis patients: a multicenter prospective cohort study. 老年维持性血液透析患者跌倒风险预测模型的构建与验证:一项多中心前瞻性队列研究
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/0886022X.2025.2455524
Lin Li, Wenbin Xu, Yiqian Fang, Qian Jiang, Yanfei Zhou, Yan Chen, Qian Yang
{"title":"Construction and validation of a fall risk prediction model in elderly maintenance hemodialysis patients: a multicenter prospective cohort study.","authors":"Lin Li, Wenbin Xu, Yiqian Fang, Qian Jiang, Yanfei Zhou, Yan Chen, Qian Yang","doi":"10.1080/0886022X.2025.2455524","DOIUrl":"10.1080/0886022X.2025.2455524","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze the risk factors for falls in elderly maintenance hemodialysis patients, construct a nomogram prediction model and validate the application.</p><p><strong>Background: </strong>Elderly maintenance hemodialysis patients face a high risk of falls, and there are fewer and less effective fall-specific assessment tools.</p><p><strong>Method: </strong>A total of 871 elderly hemodialysis patients from 9 hospitals in Chengdu City from October 2023 to December 2024 were selected as the study objects. Baseline characteristics and fall outcomes of patients in the fall group and non-fall group were recorded and compared through 6-month follow-up. Multivariable logistic regression analysis was employed to identify independent risk factors, and construct the nomogram prediction model and complete the internal verification of the model. 218 elderly maintenance hemodialysis patients from three other hospitals in Chengdu City were selected for a 6-month follow-up of falls from January to February 2024 to complete the external validation of the model.</p><p><strong>Result: </strong>The incidence of falls in elderly maintenance hemodialysis patients was 31.96%, and logistic regression analysis showed that age, sex, visual impairment, intradialytic hypotension, cognitive impairment and depression were independent risk factors for falls. Both internal and external validation of the model demonstrated area under the curve greater than 0.80. Furthermore, calibration plots, the Hosmer-Lemeshow test, and clinical decision curves all demonstrated that the model had good calibration and clinical utility.</p><p><strong>Conclusion: </strong>The nomogram constructed based on the above risk factors can provide scientific basis and practical tools for early clinical identification of high-risk groups of falls.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2455524"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The prognostic value of the neutrophil-percentage-to-albumin ratio for all-cause and cardiovascular mortality in chronic kidney disease stages G3a to G5: insights from NHANES 2003-2018. 中性粒细胞百分比与白蛋白比率对G3a至G5期慢性肾脏疾病全因死亡率和心血管死亡率的预后价值:来自NHANES 2003-2018的见解
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-05-07 DOI: 10.1080/0886022X.2025.2495861
Jialing Rao, Yuanqing Li, Xiaohao Zhang, Wenbo Zhao, Yanru Chen, Jun Zhang, Hui Peng, Zengchun Ye
{"title":"The prognostic value of the neutrophil-percentage-to-albumin ratio for all-cause and cardiovascular mortality in chronic kidney disease stages G3a to G5: insights from NHANES 2003-2018.","authors":"Jialing Rao, Yuanqing Li, Xiaohao Zhang, Wenbo Zhao, Yanru Chen, Jun Zhang, Hui Peng, Zengchun Ye","doi":"10.1080/0886022X.2025.2495861","DOIUrl":"https://doi.org/10.1080/0886022X.2025.2495861","url":null,"abstract":"<p><strong>Background: </strong>Patients with chronic kidney disease (CKD) stages G3a to G5 frequently experience heightened systemic inflammation and nutritional loss. Identifying laboratory-accessible, cost-effective markers that can effectively predict the prognosis of CKD stages G3a to G5 is crucial.</p><p><strong>Methods: </strong>This prospective cohort study included 3,331 patients with CKD stages G3a to G5 who participated in the National Health and Nutrition Examination Survey (NHANES) from 2003 to 2018. Multivariable adjusted Cox proportional hazards regression models and restricted cubic spline analyses were used to assess the associations of neutrophil percentage-to-albumin ratio (NPAR) levels with all-cause mortality, CVD, and non-CVD mortality.</p><p><strong>Results: </strong>The cohort study encompassed data from 3,331 participants for analysis. Nonlinear J-shaped associations were observed between NPAR levels and the risk of all-cause, CVD, and non-CVD mortality in patients with CKD stages G3a to G5. High levels NPAR exhibited a significantly elevated risk of both all-cause and CVD mortality in the fully adjusted model. The respective hazard ratios (HRs) for all-cause mortality were 1.23 [95% confidence interval (CI), 1.05-1.44], and for CVD mortality, 1.513 (95% CI, 1.131-2.024).</p><p><strong>Conclusions: </strong>Elevated NPAR can predict both all-cause and CVD deaths in advanced CKD patients. Individuals with high NPAR levels face an elevated risk of mortality and exhibit a decreased survival rate in the context of CKD. This finding offers evidence supporting the timely evaluation and intervention for inflammation and nutritional status in individuals with CKD stages G3a to G5.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2495861"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The significance of matrix remodeling associated 7 (MXRA7) in pathogenesis or management of renal diseases deserves more investigations. 基质重塑相关蛋白7 (matrix remodeling associated 7, MXRA7)在肾脏疾病的发病和治疗中的意义值得进一步探讨。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-01-08 DOI: 10.1080/0886022X.2024.2449575
Yiqiang Wang
{"title":"The significance of matrix remodeling associated 7 (MXRA7) in pathogenesis or management of renal diseases deserves more investigations.","authors":"Yiqiang Wang","doi":"10.1080/0886022X.2024.2449575","DOIUrl":"10.1080/0886022X.2024.2449575","url":null,"abstract":"","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2449575"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retraction: Hirudin ameliorates immunoglobulin a nephropathy by inhibition of fibrosis and inflammatory response. 撤回:水蛭素通过抑制纤维化和炎症反应改善免疫球蛋白a肾病。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/0886022X.2025.2462380
{"title":"Retraction: Hirudin ameliorates immunoglobulin a nephropathy by inhibition of fibrosis and inflammatory response.","authors":"","doi":"10.1080/0886022X.2025.2462380","DOIUrl":"10.1080/0886022X.2025.2462380","url":null,"abstract":"","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2462380"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The association between dietary live microbe intake and risk of chronic kidney disease among US adults: a cross-sectional survey from NHANES (2001-2018). 美国成年人饮食活微生物摄入量与慢性肾脏疾病风险之间的关系:来自NHANES的横断面调查(2001-2018)。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-04-15 DOI: 10.1080/0886022X.2025.2488236
Xingzi Liu, Yang Shen, Kaiyi Zhu, Meiling Jin, Qianmei Sun
{"title":"The association between dietary live microbe intake and risk of chronic kidney disease among US adults: a cross-sectional survey from NHANES (2001-2018).","authors":"Xingzi Liu, Yang Shen, Kaiyi Zhu, Meiling Jin, Qianmei Sun","doi":"10.1080/0886022X.2025.2488236","DOIUrl":"https://doi.org/10.1080/0886022X.2025.2488236","url":null,"abstract":"<p><strong>Background: </strong>Previous studies have suggested that gut dysbacteriosis may promote the onset of chronic kidney disease (CKD). However, the relationship between consumption of live microorganisms and CKD remains unclear. This study aimed to evaluate the association between dietary consumption of live microorganisms and risk of CKD.</p><p><strong>Methods: </strong>We conducted a cross-sectional analysis using data from the National Health and Nutrition Examination Survey (NHANES) spanning 2001 to 2018. Dietary intake was assessed through self-reported questionnaires, while CKD diagnosis was based on glomerular filtration rate and albumin-creatinine ratio measurements.</p><p><strong>Results: </strong>After adjusting for potential confounders, participants with high live microbial intake had a significantly lower risk of CKD compared to those with low intake [odds ratio (OR): 0.79, 95% confidence interval (CI): 0.68-0.91, <i>p</i> = 0.001]. Similarly, those with moderate/high live microbial intake exhibited a reduced CKD risk compared to the low intake group (OR: 0.87, 95% CI: 0.78-0.97, <i>p</i> = 0.009). Subgroup analyses revealed a significant interaction between live microbial intake and CKD risk among participants with less than a high school education, as well as among Mexican Americans and other racial groups (including multiracial) (all P values for interaction < 0.05). A U-shaped dose-response relationship was identified between microbial intake and CKD risk, with significant non-linear associations observed for high consumption levels (P for non-linearity = 0.013).</p><p><strong>Conclusions: </strong>High dietary intake of live microorganisms is associated with a lower risk of CKD, highlighting the potential role of gut microbiota modulation in CKD prevention.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2488236"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Artificial intelligence in kidney transplantation: a 30-year bibliometric analysis of research trends, innovations, and future directions. 肾移植中的人工智能:30年研究趋势、创新和未来方向的文献计量学分析。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-02-05 DOI: 10.1080/0886022X.2025.2458754
Ying Jia He, Pin Lin Liu, Tao Wei, Tao Liu, Yi Fei Li, Jing Yang, Wen Xing Fan
{"title":"Artificial intelligence in kidney transplantation: a 30-year bibliometric analysis of research trends, innovations, and future directions.","authors":"Ying Jia He, Pin Lin Liu, Tao Wei, Tao Liu, Yi Fei Li, Jing Yang, Wen Xing Fan","doi":"10.1080/0886022X.2025.2458754","DOIUrl":"10.1080/0886022X.2025.2458754","url":null,"abstract":"<p><p>Kidney transplantation is the definitive treatment for end-stage renal disease (ESRD), yet challenges persist in optimizing donor-recipient matching, postoperative care, and immunosuppressive strategies. This study employs bibliometric analysis to evaluate 890 publications from 1993 to 2023, using tools such as CiteSpace and VOSviewer, to identify global trends, research hotspots, and future opportunities in applying artificial intelligence (AI) to kidney transplantation. Our analysis highlights the United States as the leading contributor to the field, with significant outputs from Mayo Clinic and leading authors like Cheungpasitporn W. Key research themes include AI-driven advancements in donor matching, deep learning for post-transplant monitoring, and machine learning algorithms for personalized immunosuppressive therapies. The findings underscore a rapid expansion in AI applications since 2017, with emerging trends in personalized medicine, multimodal data fusion, and telehealth. This bibliometric review provides a comprehensive resource for researchers and clinicians, offering insights into the evolution of AI in kidney transplantation and guiding future studies toward transformative applications in transplantation science.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2458754"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of intensive water-salt diet nursing intervention on blood pressure and volume load in patients with chronic renal failure. 强化水盐饮食护理干预对慢性肾功能衰竭患者血压和容量负荷的影响。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-03-11 DOI: 10.1080/0886022X.2025.2474854
Liyan Wu, Wanli Ma, Hui Zhang, Ting Yang, Mengxi Sun, Zhen Yang, Xiaohan Guo
{"title":"Effect of intensive water-salt diet nursing intervention on blood pressure and volume load in patients with chronic renal failure.","authors":"Liyan Wu, Wanli Ma, Hui Zhang, Ting Yang, Mengxi Sun, Zhen Yang, Xiaohan Guo","doi":"10.1080/0886022X.2025.2474854","DOIUrl":"10.1080/0886022X.2025.2474854","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to investigate the impact of a comprehensive nursing intervention targeting high water and salt intake on blood pressure and volume burden in patients with chronic renal failure.</p><p><strong>Method: </strong>From January 2020 to January 2023, 120 patients diagnosed with chronic renal failure were treated at our hospital. Participants were randomly allocated to either a control group (<i>n</i> = 60) receiving standard dietary education or an observation group (<i>n</i> = 60) receiving intensive water-salt diet nursing intervention alongside standard education. Blood pressure, volume load, and related parameters were compared after a 6-month observation period.</p><p><strong>Result: </strong>Both groups exhibited reduced systolic and diastolic blood pressure post-intervention (<i>p</i> < 0.05). The observation group demonstrated a significantly lower extracellular water-to-total body water ratio (ECW/TBW) compared to the control group (<i>p</i> < 0.05). The observation group also showed higher 24-hour urine volume (<i>p</i> < 0.05), hemoglobin levels, creatinine clearance rates (<i>p</i> < 0.05), and treatment compliance (<i>p</i> < 0.05), alongside a lower complication rate (3.33% vs. 13.33%; χ<sup>2</sup> = 3.927, <i>p</i> < 0.05). A negative correlation was observed between the Therapeutic Intervention Scoring System (TISS) scale and post-intervention blood pressure/volume load (r = -2.924, -2.184; <i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Intensive water-salt diet nursing interventions effectively control blood pressure, reduce volume load, and mitigate complications in chronic renal failure patients. This approach should be widely implemented in clinical practice.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2474854"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
USP9X suppresses ferroptosis in diabetic kidney disease by deubiquitinating Nrf2 in vitro. USP9X通过体外去泛素化Nrf2抑制糖尿病肾病的铁下垂。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-02-18 DOI: 10.1080/0886022X.2025.2458761
Ningjun Shao, Kedan Cai, Yue Hong, Lingping Wu, Qun Luo
{"title":"USP9X suppresses ferroptosis in diabetic kidney disease by deubiquitinating Nrf2 <i>in vitro</i>.","authors":"Ningjun Shao, Kedan Cai, Yue Hong, Lingping Wu, Qun Luo","doi":"10.1080/0886022X.2025.2458761","DOIUrl":"10.1080/0886022X.2025.2458761","url":null,"abstract":"<p><p>Nuclear factor erythroid 2-related factor 2 (Nrf2) regulates many critical genes associated with iron storage and transportation, the activity of which is influenced by E3 ligase-mediated ubiquitination. We wondered whether there is a deubiquitinase that mediates the deubiquitination of Nrf2 to stabilize Nrf2 expression and further prevent diabetic kidney disease (DKD). High glucose (HG) was applied to induce an <i>in vitro</i> model of DKD. The effects of HG on HK-2 cell viability, apoptosis, Fe<sup>2+</sup> level, Nrf2, and ubiquitin-specific protease 9X (USP9X) were assessed by cell counting kit-8 (CCK-8) assay, flow cytometry, iron assay, and Western blot. The direct interaction between Nrf2 and USP9X was analyzed using co-immunoprecipitation and ubiquitination assay. After transfection and ferrostatin-1 (Fer-1) intervention, Nrf2 and USP9X levels, cell viability, apoptosis, and Fe<sup>2+</sup> level were tested again. Reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH) contents, and ferroptosis-related markers were assessed by ROS assay kit, ELISA, and Western blot. HG reduced cell viability and levels of USP9X and Nrf2, while elevating apoptosis and Fe<sup>2+</sup> level. An interaction between USP9X and Nrf2 has been verified and USP9X deubiquitinated Nrf2. Nrf2 up-regulation augmented the viability, GSH content, and ferroptosis-related protein expressions, while suppressing the apoptosis, Fe<sup>2+</sup> level, MDA, and ROS content in HG-mediated HK-2 cells, which was reversed by USP9X silencing. Fer-1 offset the combined modulation of Nrf2 and siUSP9X on HG-induced HK-2 cells. USP9X mediates Nrf2 deubiquitinase to hamper the ferroptosis in DKD <i>in vitro</i>.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2458761"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The m6A reader YTHDF2 protects vascular smooth muscle cells against the osteogenic differentiation through targeting Runx2. m6A读取器YTHDF2通过靶向Runx2保护血管平滑肌细胞免受成骨分化。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-04-14 DOI: 10.1080/0886022X.2025.2488876
Lanmei Li, Meijuan Cheng, Jingjing Jin, Yunfeng Zhao, Weiwei Bai, Dongxue Zhang, Shenglei Zhang, Yaling Bai, Jinsheng Xu
{"title":"The m6A reader YTHDF2 protects vascular smooth muscle cells against the osteogenic differentiation through targeting Runx2.","authors":"Lanmei Li, Meijuan Cheng, Jingjing Jin, Yunfeng Zhao, Weiwei Bai, Dongxue Zhang, Shenglei Zhang, Yaling Bai, Jinsheng Xu","doi":"10.1080/0886022X.2025.2488876","DOIUrl":"https://doi.org/10.1080/0886022X.2025.2488876","url":null,"abstract":"<p><p>Vascular calcification (VC) is an important pathological development progress in chronic kidney disease (CKD) and may increase mortality but lacks effective treatments. N6-methyladenosine (m6A) has been verified to be the most prevalent internal chemical RNA modification in mammalian mRNAs. The M6A-modified mRNA degradation process is mediated by the reader YTHDF2 in an m6A-dependent manner. Nevertheless, the exact role and molecular mechanism of YTHDF2 in VC remain unclear. This study aimed to investigate the potential role of YTHDF2 in the osteogenic differentiation of vascular smooth muscle cells (VSMCs). It was found that YTHDF2 was markedly downregulated in both <i>in vivo</i> and <i>in vitro</i> calcified models. Functionally, YTHDF2 plays a protective role in VC. The overexpression of YTHDF2 inhibited the transdifferentiation of VSMCs from a contractile to an osteogenic phenotype, thus decreasing the expression of mineralization regulatory proteins and calcium deposition. Conversely, YTHDF2 deficiency aggravated this process. At the mechanistic level, YTHDF2 suppressed osteogenic transdifferentiation of VSMCs by regulating the Runt-related transcription factor 2 (Runx2). RNA immunoprecipitation-qPCR (RIP-qPCR) confirmed the binding of YTHDF2 to Runx2, and luciferase reporter assays confirmed the presence of the m6A site in Runx2. In addition, an actinomycin D assay showed that the half-life of Runx2 mRNA was dramatically shortened in VSMCs overexpressing YTHDF2. These results suggest that YTHDF2 directly binds to the m6A modification site of Runx2 to mediate the mRNA degradation that prevents VC by inhibiting the osteogenic development of VSMCs. Therefore, YTHDF2 can be considered a potential therapeutic target for managing VC.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2488876"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive profiling of cell type-specific expression and distribution of complement genes in mouse and human kidneys: insights into normal physiology and response to kidney transplantations. 全面分析小鼠和人类肾脏中补体基因的细胞类型特异性表达和分布:洞察正常生理和肾移植反应。
IF 3 3区 医学
Renal Failure Pub Date : 2025-12-01 Epub Date: 2025-02-27 DOI: 10.1080/0886022X.2025.2471568
Xianzhi Li, Li Zhu
{"title":"Comprehensive profiling of cell type-specific expression and distribution of complement genes in mouse and human kidneys: insights into normal physiology and response to kidney transplantations.","authors":"Xianzhi Li, Li Zhu","doi":"10.1080/0886022X.2025.2471568","DOIUrl":"10.1080/0886022X.2025.2471568","url":null,"abstract":"<p><strong>Background: </strong>Recent studies innovatively revealed the localized expression of complement genes in kidneys and shed light on the vital roles of the intracellular complement system in the physiologic function and pathological conditions. However, a comprehensive analysis of the expression of complement genes in the context of the evolving cellular landscape of the kidney is not available.</p><p><strong>Methods: </strong>We analyzed single-cell RNA sequencing data from healthy human subjects, C57BL/6 mice, and kidney transplant-rejected mice. The data were sourced from the NCBI Gene Expression Omnibus and processed using quality control measures and unsupervised clustering. Differential gene analyses were based on expression levels.</p><p><strong>Results: </strong>In total, 50 complement genes were categorized into pattern recognition molecules, proteases, complement components, receptors, and regulators. In normal mice kidneys, complement genes were expressed at relatively low levels. Among different complement gene categories, receptor genes were most widely expressed in kidney cells. Comparatively, macrophages and mesangial cells are the most abundant immune and nonimmune cell types for complement gene expression. A comparison of human and mouse data showed similar expression patterns, but human kidney complement gene expression was more abundant. Comparative analysis between mouse transplant-rejected and normal kidneys demonstrated stronger complement gene expression in transplant-rejected kidneys.</p><p><strong>Conclusions: </strong>This study illustrated significant similarities in complement gene expression between murine and human kidneys and highlighted the responsive nature of complement genes to kidney injury, underscoring the dynamic nature of local complement regulation. These findings enhance our understanding of the complex regulation of the complement system within the kidney, offering insights into its role in renal disease pathogenesis.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2471568"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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