Renal Failure最新文献

{"title":"Retraction: Hirudin ameliorates immunoglobulin a nephropathy by inhibition of fibrosis and inflammatory response.","authors":"","doi":"10.1080/0886022X.2025.2462380","DOIUrl":"10.1080/0886022X.2025.2462380","url":null,"abstract":"","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2462380"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The clinical outcomes of acute kidney injury substages based on serum cystatin C in pediatric patients undergoing cardiac surgery.","authors":"Yinan Li, Dongyun Bie, Chao Xiong, Sheng Shi, Zhongrong Fang, Zhongyuan Lu, Jianhui Wang","doi":"10.1080/0886022X.2025.2466114","DOIUrl":"10.1080/0886022X.2025.2466114","url":null,"abstract":"<p><strong>Background: </strong>Multiple biomarkers have been identified by previous studies to diagnose acute kidney injury (AKI). The combination of biomarkers with conventional criteria to define AKI substages in order to identify high-risk patients and improve diagnostic accuracy was recommended. Our study aimed to explore the incidence of AKI substages defined by serum cystatin C (CysC), determine whether AKI substages diagnosed with combined CysC criteria were associated with worse outcomes.</p><p><strong>Methods: </strong>We prospectively included 2519 pediatric patients (<16 years) undergoing cardiac surgery with cardiopulmonary bypass (CPB) in our cohort between March 2022 and February 2023 in Fuwai Hospital. Demographic and clinical variables were collected. To define AKI substages, Kidney Disease: Improving Global Outcomes AKI definition (based on serum creatinine (SCr) or CysC) was used. The association between AKI exposure and outcomes including length of intensive care unit stay (LOIS), duration of mechanical ventilation (DMV), length of hospital stay (LOHS), and 30-day mortality was assessed. In addition, we determined areas under the receiver operating characteristic (ROC) curve and cutoff value of CysC preoperatively and postoperatively to predict AKI.</p><p><strong>Results: </strong>Five hundred and seven (20.8%) patients developed SCr-AKI, with 337 (13.8%) in stage 1, 77 (3.2%) in stage 2 and 93 (3.8%) in stage 3, respectively. Of the 1925 patients without SCr-AKI, 256 (14.3%) met the definition of sub-AKI. Of the 507 patients with SCr-AKI, 281 (55.4%) patients were defined as AKI substage A, while others (226, 44.6%) were defined as AKI substage B. After adjusting for body surface area, neonates, Society of Thoracic Surgeons-European Association for Cardio-Thoracic Surgery mortality score ≥ 4, previous sternotomy and CPB time > 120 min, the postoperative LOIS, LOHS, and DMV were prolonged with increasing hospitalization expense (<i>p</i> < .05) in patients with SCr-AKI and/or CysC-AKI. Meanwhile, only the hospitalization expense was increased in patients with SCr-AKI (<i>p</i> < .05) after the same adjustment. The area under curves was 0.691, 0.720, and 0.817 respectively, in ROC curves of preoperative, relative variation, or postoperative serum CysC. DeLong's test showed that postoperative serum CysC might have better diagnostic performance characteristics than preoperative or relative variation of CysC (<i>p</i> < .001), with cutoff point at 1.29 mg/dL (specificity, 0.77; sensitivity, 0.71).</p><p><strong>Conclusions: </strong>Our analysis indicates defining AKI with both CysC and SCr might more significantly affect clinical outcome associations in pediatric patients undergoing cardiac surgery. Moreover, the serum CysC cutoff of 1.29 mg/dL postoperatively is a valuable threshold for AKI risk assessment to define AKI subtypes.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2466114"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11849008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143469064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent renoprotective effects of sacubitril/valsartan in heart failure: a retrospective study.","authors":"Takahiro Kato, Yusuke Nakano, Noriko Yasukawa, Masafumi Oonishi, Yukihiro Hamada","doi":"10.1080/0886022X.2025.2538830","DOIUrl":"https://doi.org/10.1080/0886022X.2025.2538830","url":null,"abstract":"<p><p>To evaluate the dose-dependent renoprotective effects of sacubitril/valsartan in heart failure patients. This retrospective observational study included patients with heart failure (Stage B or higher, B-type natriuretic peptide (BNP) >100 pg/mL or N-terminal proBNP >300 pg/mL) who initiated sacubitril/valsartan (SV) treatment. Patients were classified by final SV daily dose (50, 100, 200, or 400 mg) at 18 months. Factors associated with eGFR changes were identified using multiple regression analysis. A total of 157 patients (mean age 74.8-77.9 years, 64.3% male) were stratified by daily SV dosage groups (50 mg, <i>n</i> = 20; 100 mg, <i>n</i> = 46; 200 mg, <i>n</i> = 62; 400 mg, <i>n</i> = 29). Baseline characteristics were similar across groups for eGFR, heart failure stage, diabetes history, myocardial infarction, atrial fibrillation, proteinuria, and use of most heart failure medications. However, hypertension prevalence and systolic blood pressure differed significantly between groups (<i>p</i> < 0.05). One-way ANOVA revealed significant dose-dependent differences in eGFR changes among SV dosage groups (<i>p</i> < 0.05). In the final multiple linear regression model, SV dosage (<i>p</i> < 0.05) was a significant factor associated with eGFR changes, with proteinuria showing a trend toward significance. Sex and BNP levels ≥400 pg/dL were not significant. Sensitivity analysis converting SV dosage to a categorical variable confirmed these findings. Stratification by proteinuria status demonstrated dose-dependent relationships in both proteinuria-positive and proteinuria-negative subgroups, with more pronounced dose dependency in the proteinuria-positive group (<i>p</i> < 0.001). SV exhibits dose-dependent renoprotective effects in heart failure patients. Optimizing SV dosage may be beneficial for heart failure patients with concurrent kidney dysfunction, especially those with proteinuria.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2538830"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A practical guide for nephrologist peer reviewers: evaluating artificial intelligence and machine learning research in nephrology.","authors":"Yanni Wang, Wisit Cheungpasitporn, Hatem Ali, Jianbo Qing, Charat Thongprayoon, Wisit Kaewput, Karim M Soliman, Zhengxing Huang, Min Yang, Zhongheng Zhang","doi":"10.1080/0886022X.2025.2513002","DOIUrl":"10.1080/0886022X.2025.2513002","url":null,"abstract":"<p><p>Artificial intelligence (AI) and machine learning (ML) are transforming nephrology by enhancing diagnosis, risk prediction, and treatment optimization for conditions such as acute kidney injury (AKI) and chronic kidney disease (CKD). AI-driven models utilize diverse datasets-including electronic health records, imaging, and biomarkers-to improve clinical decision-making. Applications such as convolutional neural networks for kidney biopsy interpretation, and predictive modeling for renal replacement therapies underscore AI's potential. Nonetheless, challenges including data quality, limited external validation, algorithmic bias, and poor interpretability constrain the clinical reliability of AI/ML models. To address these issues, this article offers a structured framework for nephrologist peer reviewers, integrating the TRIPOD-AI (Transparent Reporting of a Multivariable Prediction Model for Individual Prognosis or Diagnosis-AI Extension) checklist. Key evaluation criteria include dataset integrity, feature selection, model validation, reporting transparency, ethics, and real-world applicability. This framework promotes rigorous peer review and enhances the reproducibility, clinical relevance, and fairness of AI research in nephrology. Moreover, AI/ML studies must confront biases-data, selection, and algorithmic-that adversely affect model performance. Mitigation strategies such as data diversification, multi-center validation, and fairness-aware algorithms are essential. Overfitting in AI is driven by small patient cohorts faced with thousands of candidate features; our framework spotlights this imbalance and offers concrete remedies. Future directions in AI-driven nephrology include multimodal data fusion for improved predictive modeling, deep learning for automated imaging analysis, wearable-based monitoring, and clinical decision support systems (CDSS) that integrate comprehensive patient data. A visual summary of key manuscript sections is included.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2513002"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smurf2 knockdown attenuates the progression of diabetic nephropathy by inhibiting mesangial cell proliferation and fibrosis through suppressing EYA2 ubiquitination.","authors":"Lizhen Chen, Yuqing Chen, Fei Liu, Yinhao Liu","doi":"10.1080/0886022X.2025.2520904","DOIUrl":"https://doi.org/10.1080/0886022X.2025.2520904","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is a serious microvascular complication of diabetes and the main cause of end-stage renal disease. Smurf2 is a member of ubiquitin ligases. In this study, we aimed to investigate the mechanism by which Smurf2 mediated the development of DN. C57BL/6 mice were intraperitoneally injected into streptozotocin to construct a DN mouse model, and mouse mesangial cells (MCs) were treated with high glucose (HG) to establish a cell model. A cell counting kit 8, EDU staining and Western blot assay were performed to assess cell proliferation and fibrosis. The interaction between Smurf2 and EYA2 was identified by immunoprecipitation, and the ubiquitination of EYA2 was detected by Western blot. In addition, the kidney injury of DN mice was evaluated by hematoxylin-eosin and Masson staining and the detection of biochemical parameters. Results suggested that Smurf2 expression was increased in DN mouse model and HG-treated MCs; Smurf2 knockdown inhibited cell proliferation and fibrosis in HG-treated MCs. Mechanically, Smurf2 knockdown inhibited the ubiquitination on EYA2, leading to the suppression of EYA2 degradation and an upregulation in EYA2 protein levels. Moreover, EYA2 knockdown restored cell proliferation and fibrosis in HG-treated MCs inhibited by Smurf2 knockdown. Additionally, Smurf2 knockdown inhibited kidney injury and fibrosis in DN mouse model. In conclusion, we demonstrated that Smurf2 knockdown attenuated the progression of DN by inhibiting MCs proliferation and fibrosis through suppressing EYA2 ubiquitination, which may provide a novel insight into the pathogenesis of DN.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2520904"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal relationships between gut microbiota and IgA nephropathy: evidence from Mendelian randomization and microbiome validation.","authors":"Xin Wang, Jiong Liu, Wuda Huoshen, Jing Liu, Xue Qiao, Hong Zhang, Xu-Jie Zhou","doi":"10.1080/0886022X.2025.2522979","DOIUrl":"https://doi.org/10.1080/0886022X.2025.2522979","url":null,"abstract":"<p><strong>Background: </strong>Emerging evidence links gut microbiota strongly with IgA Nephropathy (IgAN). However, the causal role of specific gut microbiota in IgAN remains unclear. This study used a two-sample Mendelian randomization (MR) approach, validated with 16S rRNA datasets, to identify these causal relationships.</p><p><strong>Methods: </strong>We performed MR analysis using genetic instruments for 412 gut microbiota taxa from genome-wide association studies (GWAS) as exposures and IgAN GWAS data as outcomes. The inverse-variance weighted method was used as the primary analysis, supplemented by MR-Egger regression, weighted median methods, and Cochran's Q test to assess pleiotropy and heterogeneity. Significant findings were validated using reverse, multivariable, and mediation MR analyses. Results were validated using genus-level 16S rRNA datasets with batch correction (ConQuR), and microbial function was inferred <i>via</i> PICRUSt2.</p><p><strong>Results: </strong>Three gut microbiota species were protective against IgAN: <i>s_Alistipes_senegalensis</i> (OR = 0.64, <i>p</i> = .002), <i>s_Ruminococcus_bromii</i> (OR = 0.75, <i>p</i> = .040), and <i>s_Bilophila_unclassified</i> (OR = 0.68, <i>p</i> = .040). Six species were associated with increased IgAN risk, including <i>g_Barnesiella</i> (OR = 1.32, <i>p</i> = .030) and <i>s_Rothia_mucilaginosa</i> (OR = 1.52, <i>p</i> = .040). After multiple-testing correction, significant associations persisted for <i>s_Alistipes_senegalensis</i> (<i>p</i> = .043), <i>s_Bacteroides_clarus</i> (<i>p</i> = .035), and <i>s_Bilophila_unclassified</i> (<i>p</i> = .002). Sensitivity analyses confirmed robust results without pleiotropy or heterogeneity. Genus-level validation confirmed consistent microbial shifts. Functional predictions showed upregulation of carbohydrate/fatty acid metabolism and downregulation of the urea cycle.</p><p><strong>Conclusions: </strong>This study reveals specific gut microbes and metabolic pathways potentially driving IgAN, offering novel biomarkers and therapeutic targets for microbiome-based interventions.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2522979"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variations in acute kidney injury incidence and outcomes: a multicenter prospective observational study highlighting socioeconomic disparities.","authors":"Ali AlSahow, Omar Alkandari, Naser AlSabti, Bassam AlHelal, Anas AlYousef, Heba AlRajab, Ahmed AlQallaf, Yousif Bahbahani, Monther AlSharekh, Abdulrahman AlKandari, Gamal Nessim, Bassem Mashal, Ahmad Mazroue, Alaa Abdelmoteleb, Mohamed Saad, Ali Abdelzaher, Emad Abdallah, Mohamed Abdellatif, Ziad ElHusseini, Ahmed Abdelrady","doi":"10.1080/0886022X.2025.2520421","DOIUrl":"10.1080/0886022X.2025.2520421","url":null,"abstract":"<p><strong>Background: </strong>Kuwait experiences cool winters and hot summers. We evaluated the impact of ambient temperature in these two seasons on acute kidney injury (AKI) incidence and outcomes, and assessed difference between Kuwaitis and non-Kuwaitis.</p><p><strong>Method: </strong>Clinical and 30-day outcome data from AKI patients who were admitted to seven public hospitals during winter and summer of 2021 were prospectively collected.</p><p><strong>Results: </strong>Total number of AKI cases during both seasons was 1,493. Incidence was same in both seasons (50.0% each). Kuwaitis accounted for 56.7% of cases. Most AKI cases for Kuwaitis occurred in winter (52.4%), while most for non-Kuwaitis occurred in summer (53.2%). AKI patients in winter were significantly older (64.8 vs. 62.0 years, <i>p</i> = 0.001), had lower baseline eGFR (57.7 vs. 69.4 mL/min/1.73 m², <i>p</i> < 0.001), and had more cardiovascular (60.1% vs. 50.6%, <i>p</i> < 0.001), and chronic kidney diseases (59.3% vs. 43.6%, <i>p</i> < 0.001). Fluid utilization was higher in summer (83.1% vs. 75.3%, <i>p</i> < 0.001). No difference in mechanical ventilation and dialysis reported. Dialysis utilized slightly more frequently in summer (24.8% vs. 27.3%, <i>p</i> = 0.6), with significantly higher dialysis utilization for non-Kuwaitis in summer (30.6% vs. 23.0% for Kuwaitis, <i>p</i> < 0.001). Mortality rate was 26.1%, and complete kidney recovery occurred in 56.1% of cases with no difference between groups.</p><p><strong>Conclusion: </strong>No seasonal variations in AKI incidence, dialysis need, or mortality rate. In winter, AKI occurred more in older with more comorbidities among Kuwaitis but better socioeconomically, while in summer, AKI occurred more in younger, healthier non-Kuwaitis but socioeconomically disadvantaged.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2520421"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of rapamycin on delayed graft function in kidney transplant recipients: a meta-analysis.","authors":"Yan Tang, Jiefu Zhu, Xiaolan Mao, Zhitao Cai","doi":"10.1080/0886022X.2025.2515530","DOIUrl":"10.1080/0886022X.2025.2515530","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of rapamycin on DFG (delayed graft function in kidney transplant) recipients through a systematic review and meta-analysis.</p><p><strong>Methods: </strong>We searched PubMed, Embase, Cochrane Library, and other databases for studies assessing rapamycin use in kidney transplantation with a focus on DGF. The search was conducted from the time of database construction to December 2024. Literature search and quality evaluation were conducted by two researchers. Data were analyzed using RevMan 5.3, with odds ratio (OR) for dichotomous outcomes and mean differences (MD) for continuous outcomes. The meta-analysis was performed with Q-<i>I</i>²; fixed model for <i>I</i>² < 50%; sensitivity analysis for <i>I</i>² ≥ 50%. <i>p</i> Values < 0.05 were considered statistically significant.</p><p><strong>Results: </strong>Nine studies (<i>n</i> = 9,219) were included. Rapamycin was associated with an increased risk of DGF (OR = 1.29, 95% CI: 1.04-1.58), with a prolonged DGF duration (MD = 8.86, 95% CI: 3.84-13.89). No significant differences were found in graft survival (OR = 1.40, 95% CI: 0.72-2.73); patient survival (OR = 1.89, 95% CI: 0.84-4.26), or rejection incidence (OR = 1.22, 95% CI: 0.78-1.90).</p><p><strong>Conclusions: </strong>Rapamycin significantly increases the risk and duration of DGF after kidney transplantation. However, it does not appear to affect long-term outcomes such as graft survival or rejection rates. These findings suggest that rapamycin should be used cautiously in transplant recipients at risk for DGF, and further studies are needed to optimize immunosuppressive strategies for this population.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2515530"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12217102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing preparation of renal biopsy specimens for TEM: a time-efficient approach to rapid diagnosis of kidney diseases.","authors":"Gen Wang, Xiaodong Zhu, Shaoshan Liang, Feng Xu, Dandan Liang, Caihong Zeng","doi":"10.1080/0886022X.2025.2522325","DOIUrl":"10.1080/0886022X.2025.2522325","url":null,"abstract":"<p><p>Electron microscopic examination of renal biopsies is crucial for the diagnosis of kidney disease. In this study, we present an optimized protocol that significantly reduces processing time to 19.5 h, compared to 120 h with conventional methods, while preserving ultrastructural quality in 55 renal biopsy specimens. The modified method incorporates the use of higher concentrations of fixatives, shortened fixation duration, low-viscosity resin embedding, and the application of uncoated copper grids. Comparative analysis of renal specimens processed using conventional and optimized methods demonstrated equivalent preservation of critical ultrastructural features, including the glomerular basement membranes, podocytes and tubular epithelium. The total processing time was markedly reduced to 19.5 h, compared to several days with conventional methods. This rapid protocol enables timely ultrastructural evaluation of renal biopsies to facilitate prompt diagnosis and decision-making, highlighting the significance of optimizing diagnostic pathology methodologies for enhancing efficiency and maintaining accuracy.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2522325"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics reveals TNFAIP6 as a candidate gene and suggests its potential crosstalk in the treatment of hemodialysis in chronic kidney disease.","authors":"Guoxin Zhang, Jieqiong Fu, Wenming Niu","doi":"10.1080/0886022X.2025.2528757","DOIUrl":"10.1080/0886022X.2025.2528757","url":null,"abstract":"<p><p>Hemodialysis (HD) is a life-sustaining treatment for chronic kidney disease (CKD) patients. This study aimed to identify candidate diagnostic biomarkers associated with HD-treated CKD. The public dataset was acquired from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) showing opposite trends across the three groups were obtained as crosstalk genes, and their potential pathways were explored through KEGG and GSEA analyses. Next, hub genes were identified using LASSO regression, and their diagnostic potential was assessed <i>via</i> ROC analysis. Key immune cell populations were identified using ssGSEA. Blood samples from healthy controls (<i>n</i> = 10), CKD patients (<i>n</i> = 9), and HD-treated CKD patients (<i>n</i> = 7) were collected to validate hub gene expression. A total of 132 crosstalk genes were identified, with the TGF-β and KRAS signaling pathways potentially activated in the HD group. Two hub genes, SIK1 and TNFAIP6, exhibited AUC values exceeding 0.8 for diagnosing CKD and HD-treated CKD groups. Compared to the other groups, neutrophil abundance was significantly higher in CKD group and showed a strong correlation with the hub genes. External datasets and RT-qPCR validated a consistent expression trend of TNFAIP6. Therefore, TNFAIP6 may represent a potential candidate gene with biomarker relevance in CKD and HD-treated CKD. TNFAIP6 has been previously associated with the TGF-β pathway and neutrophil regulation, and its crosstalk mechanism in HD-treated CKD warrants further exploration.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":"47 1","pages":"2528757"},"PeriodicalIF":3.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}