Protein & Cell最新文献_第9页

Identification of FOXO1 as a geroprotector in human synovium through single-nucleus transcriptomic profiling. 通过单核转录组分析确定 FOXO1 是人类滑膜中的老年保护因子

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-05-28 DOI: 10.1093/procel/pwad060

Feifei Liu, Yi Lu, Xuebao Wang, Shuhui Sun, Huize Pan, Min Wang, Zehua Wang, Weiqi Zhang, Shuai Ma, Guoqiang Sun, Qun Chu, Si Wang, Jing Qu, Guang-Hui Liu

{"title":"Identification of FOXO1 as a geroprotector in human synovium through single-nucleus transcriptomic profiling.","authors":"Feifei Liu, Yi Lu, Xuebao Wang, Shuhui Sun, Huize Pan, Min Wang, Zehua Wang, Weiqi Zhang, Shuai Ma, Guoqiang Sun, Qun Chu, Si Wang, Jing Qu, Guang-Hui Liu","doi":"10.1093/procel/pwad060","DOIUrl":"10.1093/procel/pwad060","url":null,"abstract":"The synovium, a thin layer of tissue that is adjacent to the joints and secretes synovial fluid, undergoes changes in aging that contribute to intense shoulder pain and other joint diseases. However, the mechanism underlying human synovial aging remains poorly characterized. Here, we generated a comprehensive transcriptomic profile of synovial cells present in the subacromial synovium from young and aged individuals. By delineating aging-related transcriptomic changes across different cell types and their associated regulatory networks, we identified two subsets of mesenchymal stromal cells (MSCs) in human synovium, which are lining and sublining MSCs, and found that angiogenesis and fibrosis-associated genes were upregulated whereas genes associated with cell adhesion and cartilage development were downregulated in aged MSCs. Moreover, the specific cell-cell communications in aged synovium mirrors that of aging-related inflammation and tissue remodeling, including vascular hyperplasia and tissue fibrosis. In particular, we identified forkhead box O1 (FOXO1) as one of the major regulons for aging differentially expressed genes (DEGs) in synovial MSCs, and validated its downregulation in both lining and sublining MSC populations of the aged synovium. In human FOXO1-depleted MSCs derived from human embryonic stem cells, we recapitulated the senescent phenotype observed in the subacromial synovium of aged donors. These data indicate an important role of FOXO1 in the regulation of human synovial aging. Overall, our study improves our understanding of synovial aging during joint degeneration, thereby informing the development of novel intervention strategies aimed at rejuvenating the aged joint.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"441-459"},"PeriodicalIF":13.6,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11131031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138807228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single transmembrane GPCR modulating proteins: neither single nor simple. 单跨膜 GPCR 调节蛋白：既不单一也不简单。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-05-28 DOI: 10.1093/procel/pwad035

Meng Wang, Jianjun Lyu, Chao Zhang

引用次数: 0

Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1. 微生物群肠毒性脆弱拟杆菌分泌的 BFT-1 通过其功能受体 NOD1 促进乳腺癌细胞的干性和化疗抗性。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-05-28 DOI: 10.1093/procel/pwae005

Wei Ma, Lu Zhang, Weilong Chen, Zhaoxia Chang, Juchuanli Tu, Yuanyuan Qin, Yuwen Yao, Mengxue Dong, Jiajun Ding, Siqin Li, Fengkai Li, Qiaodan Deng, Yifei Yang, Tingting Feng, Fanrong Zhang, Xiying Shao, Xueyan He, Lixing Zhang, Guohong Hu, Quentin Liu, Yi-Zhou Jiang, Shu Zhu, Zhi Xiao, Dan Su, Tong Liu, Suling Liu

{"title":"Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1.","authors":"Wei Ma, Lu Zhang, Weilong Chen, Zhaoxia Chang, Juchuanli Tu, Yuanyuan Qin, Yuwen Yao, Mengxue Dong, Jiajun Ding, Siqin Li, Fengkai Li, Qiaodan Deng, Yifei Yang, Tingting Feng, Fanrong Zhang, Xiying Shao, Xueyan He, Lixing Zhang, Guohong Hu, Quentin Liu, Yi-Zhou Jiang, Shu Zhu, Zhi Xiao, Dan Su, Tong Liu, Suling Liu","doi":"10.1093/procel/pwae005","DOIUrl":"10.1093/procel/pwae005","url":null,"abstract":"Tumor-resident microbiota in breast cancer promotes cancer initiation and malignant progression. However, targeting microbiota to improve the effects of breast cancer therapy has not been investigated in detail. Here, we evaluated the microbiota composition of breast tumors and found that enterotoxigenic Bacteroides fragilis (ETBF) was highly enriched in the tumors of patients who did not respond to taxane-based neoadjuvant chemotherapy. ETBF, albeit at low biomass, secreted the toxic protein BFT-1 to promote breast cancer cell stemness and chemoresistance. Mechanistic studies showed that BFT-1 directly bound to NOD1 and stabilized NOD1 protein. NOD1 was highly expressed on ALDH+ breast cancer stem cells (BCSCs) and cooperated with GAK to phosphorylate NUMB and promote its lysosomal degradation, thereby activating the NOTCH1-HEY1 signaling pathway to increase BCSCs. NOD1 inhibition and ETBF clearance increase the chemosensitivity of breast cancer by impairing BCSCs.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"419-440"},"PeriodicalIF":13.6,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11131025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune rebalancing at the maternal-fetal interface of maternal SARS-CoV-2 infection during early pregnancy. 孕早期母体感染 SARS-CoV-2 后母胎界面的免疫再平衡。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-05-28 DOI: 10.1093/procel/pwae006

Chenxiang Xi, Zihui Yan, Dandan Bai, Yalin Zhang, Beiying Wang, Xiaoxiao Han, Li Wu, Xiaohui Shi, Zhiyi Hu, Ming Tang, Zhongqu Su, Yingdong Liu, Binya Liu, Jiqing Yin, Hong Wang, Xiaocui Li, Yanping Zhang, Shaorong Gao, Wenqiang Liu

{"title":"Immune rebalancing at the maternal-fetal interface of maternal SARS-CoV-2 infection during early pregnancy.","authors":"Chenxiang Xi, Zihui Yan, Dandan Bai, Yalin Zhang, Beiying Wang, Xiaoxiao Han, Li Wu, Xiaohui Shi, Zhiyi Hu, Ming Tang, Zhongqu Su, Yingdong Liu, Binya Liu, Jiqing Yin, Hong Wang, Xiaocui Li, Yanping Zhang, Shaorong Gao, Wenqiang Liu","doi":"10.1093/procel/pwae006","DOIUrl":"10.1093/procel/pwae006","url":null,"abstract":"The current coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) remains a threat to pregnant women. However, the impact of early pregnancy SARS-CoV-2 infection on the maternal-fetal interface remains poorly understood. Here, we present a comprehensive analysis of single-cell transcriptomics and metabolomics in placental samples infected with SARS-CoV-2 during early pregnancy. Compared to control placentas, SARS-CoV-2 infection elicited immune responses at the maternal-fetal interface and induced metabolic alterations in amino acid and phospholipid profiles during the initial weeks post-infection. However, subsequent immune cell activation and heightened immune tolerance in trophoblast cells established a novel dynamic equilibrium that mitigated the impact on the maternal-fetal interface. Notably, the immune response and metabolic alterations at the maternal-fetal interface exhibited a gradual decline during the second trimester. Our study underscores the adaptive immune tolerance mechanisms and establishment of immunological balance during the first two trimesters following maternal SARS-CoV-2 infection.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"460-473"},"PeriodicalIF":21.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11131034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SMAD2/3-SMYD2 and developmental transcription factors cooperate with cell cycle inhibitors to guide tissue formation. SMAD2/3-SMYD2 和发育转录因子与细胞周期抑制剂合作，引导组织形成。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-05-17 DOI: 10.1093/procel/pwae031

Stefania Militi, Reshma Nibhani, Martin Pook, Siim Pauklin

{"title":"SMAD2/3-SMYD2 and developmental transcription factors cooperate with cell cycle inhibitors to guide tissue formation.","authors":"Stefania Militi, Reshma Nibhani, Martin Pook, Siim Pauklin","doi":"10.1093/procel/pwae031","DOIUrl":"https://doi.org/10.1093/procel/pwae031","url":null,"abstract":"Tissue formation and organ homeostasis is achieved by precise coordination of proliferation and differentiation of stem cells and progenitors. While deregulation of these processes can result in degenerative disease or cancer, their molecular interplays remain unclear. Here we show that the switch of human pluripotent stem cell (hPSC) self-renewal to differentiation is associated with the induction of distinct cyclin dependent kinase inhibitors (CDKIs). In hPSCs, Activin/Nodal/TGFβ signalling maintains CDKIs in a poised state via SMAD2/3-NANOG-OCT4-EZH2-SNON transcriptional complex. Upon gradual differentiation, CDKIs are induced by successive transcriptional complexes between SMAD2/3-SMYD2 and developmental regulators such as EOMES, thereby lengthening the G1 phase. This, in turn, induces SMAD2/3 transcriptional activity by blocking its linker phosphorylation. Such SMAD2/3-CDKI positive feedback loops drive the exit from pluripotency and stepwise cell fate specification that could be harnessed for producing cells for therapeutic applications. Our study uncovers fundamental mechanisms how cell fate specification is interconnected to cell cycle dynamics and provides insight to autonomous circuitries governing tissue self-formation.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":""},"PeriodicalIF":21.1,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring unconventional attributes of red blood cells and their potential applications in biomedicine. 探索红细胞的非传统属性及其在生物医学中的潜在应用。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-05-07 DOI: 10.1093/procel/pwae001

Alkmini T Anastasiadi, Vasiliki-Zoi Arvaniti, Krystalyn E Hudson, Anastasios G Kriebardis, Constantinos Stathopoulos, Angelo D'Alessandro, Steven L Spitalnik, Vassilis L Tzounakas

引用次数: 0

Applications of genetic code expansion technology in eukaryotes. 遗传密码扩展技术在真核生物中的应用。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-05-07 DOI: 10.1093/procel/pwad051

Qiao-Ru Guo, Yu J Cao

引用次数: 0

Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics. 时空转录组学揭示灵长类卵巢衰老特征

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-05-07 DOI: 10.1093/procel/pwad063

Huifen Lu, Ying Jing, Chen Zhang, Shuai Ma, Weiqi Zhang, Daoyuan Huang, Bin Zhang, Yuesheng Zuo, Yingying Qin, Guang-Hui Liu, Yang Yu, Jing Qu, Si Wang

{"title":"Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics.","authors":"Huifen Lu, Ying Jing, Chen Zhang, Shuai Ma, Weiqi Zhang, Daoyuan Huang, Bin Zhang, Yuesheng Zuo, Yingying Qin, Guang-Hui Liu, Yang Yu, Jing Qu, Si Wang","doi":"10.1093/procel/pwad063","DOIUrl":"10.1093/procel/pwad063","url":null,"abstract":"The ovary is indispensable for female reproduction, and its age-dependent functional decline is the primary cause of infertility. However, the molecular basis of ovarian aging in higher vertebrates remains poorly understood. Herein, we apply spatiotemporal transcriptomics to benchmark architecture organization as well as cellular and molecular determinants in young primate ovaries and compare these to aged primate ovaries. From a global view, somatic cells within the non-follicle region undergo more pronounced transcriptional fluctuation relative to those in the follicle region, likely constituting a hostile microenvironment that facilitates ovarian aging. Further, we uncovered that inflammation, the senescent-associated secretory phenotype, senescence, and fibrosis are the likely primary contributors to ovarian aging (PCOA). Of note, we identified spatial co-localization between a PCOA-featured spot and an unappreciated MT2 (Metallothionein 2) highly expressing spot (MT2high) characterized by high levels of inflammation, potentially serving as an aging hotspot in the primate ovary. Moreover, with advanced age, a subpopulation of MT2high accumulates, likely disseminating and amplifying the senescent signal outward. Our study establishes the first primate spatiotemporal transcriptomic atlas, advancing our understanding of mechanistic determinants underpinning primate ovarian aging and unraveling potential biomarkers and therapeutic targets for aging and age-associated human ovarian disorders.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":" ","pages":"364-384"},"PeriodicalIF":21.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11075111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138831229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells. 更正：用源自患者的诱导多能干细胞模拟 CADASIL 血管病变。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2024-05-07 DOI: 10.1093/procel/pwad059

引用次数: 0

Degeneration Directory: a multi-omics web resource for degenerative diseases. 变性目录：退化性疾病的多组学网络资源。

IF 13.6 1区生物学

Protein & Cell Pub Date : 2024-05-07 DOI: 10.1093/procel/pwad066

Haoteng Yan, Changfa Lu, Chenyang Lan, Si Wang, Weiqi Zhang, Zan He, Jinghao Hu, Jiaqi Ai, Guang-Hui Liu, Shuai Ma, Yuanchun Zhou, Jing Qu

引用次数: 0