Protein & Cell最新文献_第9页

Strategies for generating mouse model resources of human disease. 产生人类疾病小鼠模型资源的策略。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwad011

Jirong Pan, Ling Zhang, Zhibing Huang, Dalu Zhao, He Li, Yanan Fu, Meng Wang, Borui Chen, Fuad A Iraqi, Grant Morahan, Chuan Qin

引用次数: 1

Deciphering the placental abnormalities associated with somatic cell nuclear transfer at single-nucleus resolution. 在单核分辨率下解读与体细胞核移植相关的胎盘异常。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwad030

Liyuan Jiang, Xin Wang, Leyun Wang, Sinan Ma, Yali Ding, Chao Liu, Siqi Wang, Xuan Shao, Ying Zhang, Zhikun Li, Wei Li, Guihai Feng, Qi Zhou

引用次数: 1

CRISPR-assisted transcription activation by phase-separation proteins. 通过相分离蛋白的crispr辅助转录激活。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwad013

Jiaqi Liu, Yuxi Chen, Baoting Nong, Xiao Luo, Kaixin Cui, Zhan Li, Pengfei Zhang, Wenqiong Tan, Yue Yang, Wenbin Ma, Puping Liang, Zhou Songyang

{"title":"CRISPR-assisted transcription activation by phase-separation proteins.","authors":"Jiaqi Liu, Yuxi Chen, Baoting Nong, Xiao Luo, Kaixin Cui, Zhan Li, Pengfei Zhang, Wenqiong Tan, Yue Yang, Wenbin Ma, Puping Liang, Zhou Songyang","doi":"10.1093/procel/pwad013","DOIUrl":"10.1093/procel/pwad013","url":null,"abstract":"The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms. Current CRISPR-activation (CRISPRa) platforms often require multiple components because of inefficient transcriptional activation. Here, we fused different phase-separation proteins to dCas9-VPR (dCas9-VP64-P65-RTA) and observed robust increases in transcriptional activation efficiency. Notably, human NUP98 (nucleoporin 98) and FUS (fused in sarcoma) IDR domains were best at enhancing dCas9-VPR activity, with dCas9-VPR-FUS IDR (VPRF) outperforming the other CRISPRa systems tested in this study in both activation efficiency and system simplicity. dCas9-VPRF overcomes the target strand bias and widens gRNA designing windows without affecting the off-target effect of dCas9-VPR. These findings demonstrate the feasibility of using phase-separation proteins to assist in the regulation of gene expression and support the broad appeal of the dCas9-VPRF system in basic and clinical applications.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9140892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasis. 灵长类动物睾丸衰老的单核转录组图谱揭示了精原干细胞储存库的衰竭和支持细胞稳态的丧失。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwac057

Daoyuan Huang, Yuesheng Zuo, Chen Zhang, Guoqiang Sun, Ying Jing, Jinghui Lei, Shuai Ma, Shuhui Sun, Huifen Lu, Yusheng Cai, Weiqi Zhang, Fei Gao, Andy Peng Xiang, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Jing Qu, Si Wang

{"title":"A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasis.","authors":"Daoyuan Huang, Yuesheng Zuo, Chen Zhang, Guoqiang Sun, Ying Jing, Jinghui Lei, Shuai Ma, Shuhui Sun, Huifen Lu, Yusheng Cai, Weiqi Zhang, Fei Gao, Andy Peng Xiang, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Jing Qu, Si Wang","doi":"10.1093/procel/pwac057","DOIUrl":"10.1093/procel/pwac057","url":null,"abstract":"The testis is pivotal for male reproduction, and its progressive functional decline in aging is associated with infertility. However, the regulatory mechanism underlying primate testicular aging remains largely elusive. Here, we resolve the aging-related cellular and molecular alterations of primate testicular aging by establishing a single-nucleus transcriptomic atlas. Gene-expression patterns along the spermatogenesis trajectory revealed molecular programs associated with attrition of spermatogonial stem cell reservoir, disturbed meiosis and impaired spermiogenesis along the sequential continuum. Remarkably, Sertoli cell was identified as the cell type most susceptible to aging, given its deeply perturbed age-associated transcriptional profiles. Concomitantly, downregulation of the transcription factor Wilms' Tumor 1 (WT1), essential for Sertoli cell homeostasis, was associated with accelerated cellular senescence, disrupted tight junctions, and a compromised cell identity signature, which altogether may help create a hostile microenvironment for spermatogenesis. Collectively, our study depicts in-depth transcriptomic traits of non-human primate (NHP) testicular aging at single-cell resolution, providing potential diagnostic biomarkers and targets for therapeutic interventions against testicular aging and age-related male reproductive diseases.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9476820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Cryo-EM structures of a prokaryotic heme transporter CydDC. 原核血红素转运蛋白CydDC的低温电镜结构。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwad022

Chen Zhu, Yanfeng Shi, Jing Yu, Wenhao Zhao, Lingqiao Li, Jingxi Liang, Xiaolin Yang, Bing Zhang, Yao Zhao, Yan Gao, Xiaobo Chen, Xiuna Yang, Lu Zhang, Luke W Guddat, Lei Liu, Haitao Yang, Zihe Rao, Jun Li

引用次数: 0

Architecture of severe fever with thrombocytopenia syndrome virus. 发热伴血小板减少综合征病毒的结构。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwad019

Zixian Sun, Jing Cheng, Yuan Bai, Lin Cao, Daoxin Xie, Fei Deng, Xinzheng Zhang, Zihe Rao, Zhiyong Lou

引用次数: 2

APOE-mediated suppression of the lncRNA MEG3 protects human cardiovascular cells from chronic inflammation. apoe介导的lncRNA MEG3抑制可保护人类心血管细胞免受慢性炎症的影响。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-12-01 DOI: 10.1093/procel/pwad017

Hongkai Zhao, Kuan Yang, Yiyuan Zhang, Hongyu Li, Qianzhao Ji, Zeming Wu, Shuai Ma, Si Wang, Moshi Song, Guang-Hui Liu, Qiang Liu, Weiqi Zhang, Jing Qu

引用次数: 1

Maternal and infant microbiome: next-generation indicators and targets for intergenerational health and nutrition care. 母婴微生物组:代际健康和营养保健的下一代指标和目标。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-11-08 DOI: 10.1093/procel/pwad029

Shengtao Gao, Jinfeng Wang

{"title":"Maternal and infant microbiome: next-generation indicators and targets for intergenerational health and nutrition care.","authors":"Shengtao Gao, Jinfeng Wang","doi":"10.1093/procel/pwad029","DOIUrl":"10.1093/procel/pwad029","url":null,"abstract":"Microbes are commonly sensitive to shifts in the physiological and pathological state of their hosts, including mothers and babies. From this perspective, the microbiome may be a good indicator for diseases during pregnancy and has the potential to be used for perinatal health monitoring. This is embodied in the application of microbiome from multi body sites for auxiliary diagnosis, early prediction, prolonged monitoring, and retrospective diagnosis of pregnancy and infant complications, as well as nutrition management and health products developments of mothers and babies. Here we summarized the progress in these areas and explained that the microbiome of different body sites is sensitive to different diseases and their microbial biomarkers may overlap between each other, thus we need to make a diagnosis prudently for those diseases. Based on the microbiome variances and additional anthropometric and physical data, individualized responses of mothers and neonates to meals and probiotics/prebiotics were predictable, which is of importance for precise nutrition and probiotics/prebiotics managements and developments. Although a great deal of encouraging performance was manifested in previous studies, the efficacy could be further improved by combining multi-aspect data such as multi-omics and time series analysis in the future. This review reconceptualizes maternal and infant health from a microbiome perspective, and the knowledge in it may inspire the development of new options for the prevention and treatment of adverse pregnancy outcomes and bring a leap forward in perinatal health care.","PeriodicalId":20790,"journal":{"name":"Protein & Cell","volume":null,"pages":null},"PeriodicalIF":21.1,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9464175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial sensing in the intestine. 肠道微生物感应。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-11-08 DOI: 10.1093/procel/pwad028

Tingting Wan, Yalong Wang, Kaixin He, Shu Zhu

引用次数: 2

Nutri-microbiome epidemiology, an emerging field to disentangle the interplay between nutrition and microbiome for human health. 营养-微生物组流行病学，一个新兴的领域，解开营养和微生物组之间的相互作用，为人类健康。

IF 21.1 1区生物学

Protein & Cell Pub Date : 2023-11-08 DOI: 10.1093/procel/pwad023

Wanglong Gou, Zelei Miao, Kui Deng, Ju-Sheng Zheng

引用次数: 3