PROTEOMICS – Clinical Applications最新文献_第10页

Potential functionality of Cutibacterium acnes extracellular vesicles in atopic dermatitis and acne vulgaris: A comparative proteomic analysis 痤疮杆菌胞外囊泡在特应性皮炎和寻常痤疮中的潜在功能：比较蛋白质组分析

IF 2 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-04-19 DOI: 10.1002/prca.202300106

Tianze Yu, Jin Chen, Shi Wu, Min Jiang, Ling Han, Ying Ma

{"title":"Potential functionality of Cutibacterium acnes extracellular vesicles in atopic dermatitis and acne vulgaris: A comparative proteomic analysis","authors":"Tianze Yu, Jin Chen, Shi Wu, Min Jiang, Ling Han, Ying Ma","doi":"10.1002/prca.202300106","DOIUrl":"https://doi.org/10.1002/prca.202300106","url":null,"abstract":"BackgroundCutibacterium acnes is a commensal bacterium residing in healthy skin and plays a critical role in maintaining skin homeostasis. C. acnes has been considered closely related to acne vulgaris, while recent studies suggest that C. acnes and its metabolites may have a protective role in atopic dermatitis (AD) by modulating the immune system and maintaining skin homeostasis. Extracellular vesicles (EVs) are small membranous vesicles secreted by bacteria that participate in bacteria‐host interactions.MethodsThis study first compared C. acnes EVs from AD lesions (AD‐EVs), acne lesions (Acne‐EVs), and healthy skin (NC‐EVs), using Label‐free quantitative LC‐MS/MS and validated differently expressed proteins by parallel reaction monitoring (PRM). Then Normal Human Epidermal Keratinocytes (NHEK) and human primary keratinocytes (KC) were treated with C. acnes EVs isolated from different groups, and the expressions of inflammatory factors were measured by quantitative real‐time PCR and Western blotting.ResultsCompared with the acne group, the AD group showed greater downregulation of proteins related to energy metabolism and carbon source utilization pathway. Differences in protein profile in AD and acne lesion‐separated C. acnes EVs correspond to the abnormal sebum secretion pattern in both diseases. C. acnes EVs from different groups affected different expressions of Th1 and Th2 inflammatory factors and epidermal barrier markers in NHEK and KC, indicating different immunomodulatory potentials.ConclusionsThis study observed distinct proteomic differences between AD‐EVs and Acne‐EVs, and provided insights into the functional differences of C. acnes EVs in AD and acne.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":"98 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140627933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and metabolomic characterization of bone, liver, and lung metastases in plasma of breast cancer patients 乳腺癌患者血浆中骨、肝和肺转移的蛋白质组和代谢组特征

IF 2 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-04-04 DOI: 10.1002/prca.202300136

Hui Ye, Xiabo Shen, Yaohan Li, Weibin Zou, Syed Shams ul Hassan, Yue Feng, Xiaojia Wang, Jingkui Tian, Xiying Shao, Yi Tao, Wei Zhu

{"title":"Proteomic and metabolomic characterization of bone, liver, and lung metastases in plasma of breast cancer patients","authors":"Hui Ye, Xiabo Shen, Yaohan Li, Weibin Zou, Syed Shams ul Hassan, Yue Feng, Xiaojia Wang, Jingkui Tian, Xiying Shao, Yi Tao, Wei Zhu","doi":"10.1002/prca.202300136","DOIUrl":"https://doi.org/10.1002/prca.202300136","url":null,"abstract":"BackgroundBreast cancer (BC) is the second leading cause of cancer‐related deaths among women, primarily due to metastases to other organs rather than the primary tumor.MethodsIn this study, a comprehensive analysis of plasma proteomics and metabolomics was conducted on a cohort of 51 BC patients. Potential biomarkers were screened by the Least Absolute Shrinkage and Selection Operator (LASSO) regression and Random Forest algorithm. Additionally, enzyme‐linked immunosorbent assay (ELISA) kits and untargeted metabolomics were utilized to validate the prognostic biomarkers in an independent cohort.ResultsIn the study, extracellular matrix (ECM)‐related functional enrichments were observed to be enriched in BC cases with bone metastases. Proteins dysregulated in retinol metabolism in liver metastases and leukocyte transendothelial migration in lung metastases were also identified. Machine learning models identified specific biomarker panels for each metastasis type, achieving high diagnostic accuracy with area under the curve (AUC) of 0.955 for bone, 0.941 for liver, and 0.989 for lung metastases.ConclusionsFor bone metastasis, biomarkers such as leucyl‐tryptophan, LysoPC(P‐16:0/0:0), FN1, and HSPG2 have been validated. dUDP, LPE(18:1/0:0), and aspartylphenylalanine have been confirmed for liver metastasis. For lung metastasis, dUDP, testosterone sulfate, and PE(14:0/20:5) have been established.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":"17 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masthead: Proteomics 2'24 刊头：蛋白质组学 2'24

IF 2 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-11 DOI: 10.1002/prca.202470023

引用次数: 0

Editorial Board: Proteomics 2'24 编辑委员会：蛋白质组学 2'24

IF 2 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-11 DOI: 10.1002/prca.202470022

引用次数: 0

A comparative proteomic analysis for non-invasive early prediction of hypoxic-ischemic injury in asphyxiated neonates. 无创早期预测窒息新生儿缺氧缺血性损伤的比较蛋白质组学分析。

IF 2.5 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-01 Epub Date: 2023-10-03 DOI: 10.1002/prca.202200054

Sumrati Gurtoo, Gayathree Karthikkeyan, Santosh Kumar Behera, Chinmaya Narayana Kotimoole, Mohd Altaf Najar, Prashant Kumar Modi, Sahana Ks, Sneha M Pinto, Arun Ab

{"title":"A comparative proteomic analysis for non-invasive early prediction of hypoxic-ischemic injury in asphyxiated neonates.","authors":"Sumrati Gurtoo, Gayathree Karthikkeyan, Santosh Kumar Behera, Chinmaya Narayana Kotimoole, Mohd Altaf Najar, Prashant Kumar Modi, Sahana Ks, Sneha M Pinto, Arun Ab","doi":"10.1002/prca.202200054","DOIUrl":"10.1002/prca.202200054","url":null,"abstract":"Aim: Hypoxic Ischemic Encephalopathy (HIE) is one of the principal causes of neonatal mortality and long-term morbidity worldwide. The neonatal signs of mild cerebral injury are subtle, making an early precise diagnosis difficult. Delayed detection, poor prognosis, and lack of specific biomarkers for the disease are increasing mortality rates. In this study, we intended to identify specific biomarkers using comparative proteomic analysis to predict the severity of perinatal asphyxia so that its outcome can also be prevented.Experimental design: A case-control study was conducted on 38 neonates, and urine samples were collected within 24 and 72 h of life. A tandem mass spectrometry-based quantitative proteomics approach, followed by validation via sandwich ELISA, was performed.Results: The LC-MS/MS-based proteomics analysis resulted in the identification of 1201 proteins in urine, with 229, 244, and 426 being differentially expressed in HIE-1, HIE-2, and HIE-3, respectively. Axon guidance, Diseases of programmed cell death, and Detoxification of reactive oxygen species pathways were significantly enriched in mild HIE versus severe HIE. Among the differentially expressed proteins in various stages of HIE, we chose to validate four proteins - APP, AGT, FABP1, and FN1 - via sandwich ELISA. Individual and cumulative ROC curves were plotted. AGT and FABP1 together showed high sensitivity, specificity, and accuracy as potential biomarkers for early diagnosis of HIE.Conclusion: Establishing putative urinary biomarkers will facilitate clinicians to more accurately screen neonates for brain injury and monitor the disease progression. Prompt treatment of neonates may reduce mortality and neurodevelopmental impairment.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":" ","pages":"e2200054"},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Hide and seek": Misleading transferrin variants in PMM2-CDG complicate diagnostics. “捉迷藏”:PMM2-CDG中误导性转铁蛋白变异使诊断复杂化。

IF 2.5 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-01 Epub Date: 2023-10-24 DOI: 10.1002/prca.202300040

Alexandre Raynor, Arnaud Bruneel, Pieter Vermeersch, Sophie Cholet, Sebastian Friedrich, Matthias Eckenweiler, Anke Schumann, Simone Hengst, Ali Tunç Tuncel, François Fenaille, Christian Thiel, Daisy Rymen

{"title":"\"Hide and seek\": Misleading transferrin variants in PMM2-CDG complicate diagnostics.","authors":"Alexandre Raynor, Arnaud Bruneel, Pieter Vermeersch, Sophie Cholet, Sebastian Friedrich, Matthias Eckenweiler, Anke Schumann, Simone Hengst, Ali Tunç Tuncel, François Fenaille, Christian Thiel, Daisy Rymen","doi":"10.1002/prca.202300040","DOIUrl":"10.1002/prca.202300040","url":null,"abstract":"Purpose: Congenital disorders of glycosylation (CDG) are one of the fastest growing groups of inborn errors of metabolism. Despite the availability of next-generation sequencing techniques and advanced methods for evaluation of glycosylation, CDG screening mainly relies on the analysis of serum transferrin (Tf) by isoelectric focusing, HPLC or capillary electrophoresis. The main pitfall of this screening method is the presence of Tf protein variants within the general population. Although reports describe the role of Tf variants leading to falsely abnormal results, their significance in confounding diagnosis in patients with CDG has not been documented so far. Here, we describe two PMM2-CDG cases, in which Tf variants complicated the diagnostic.Experimental design: Glycosylation investigations included classical screening techniques (capillary electrophoresis, isoelectric focusing and HPLC of Tf) and various confirmation techniques (two-dimensional electrophoresis, western blot, N-glycome, UPLC-FLR/QTOF MS with Rapifluor). Tf variants were highlighted following neuraminidase treatment. Sequencing of PMM2 was performed.Results: In both patients, Tf screening pointed to CDG-II, while second-line analyses pointed to CDG-I. Tf variants were found in both patients, explaining these discrepancies. PMM2 causative variants were identified in both patients.Conclusion and clinical relevance: We suggest that a neuraminidase treatment should be performed when a typical CDG Tf pattern is found upon initial screening analysis.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":" ","pages":"e2300040"},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50158637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human peripheral blood mononuclear cells as a valuable source of disease-related biomarkers: Evidence from comparative proteomics studies. 人类外周血单核细胞作为疾病相关生物标志物的宝贵来源：来自比较蛋白质组学研究的证据。

IF 2.5 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-01 Epub Date: 2023-11-07 DOI: 10.1002/prca.202300072

Michal Alexovič, Csilla Uličná, Ján Sabo, Katarina Davalieva

{"title":"Human peripheral blood mononuclear cells as a valuable source of disease-related biomarkers: Evidence from comparative proteomics studies.","authors":"Michal Alexovič, Csilla Uličná, Ján Sabo, Katarina Davalieva","doi":"10.1002/prca.202300072","DOIUrl":"10.1002/prca.202300072","url":null,"abstract":"Purpose: The discovery of specific and sensitive disease-associated biomarkers for early diagnostic purposes of many diseases is still highly challenging due to various complex molecular mechanisms triggered, high variability of disease-related interactions, and an overlap of manifestations among diseases. Human peripheral blood mononuclear cells (PBMCs) contain protein signatures corresponding to essential immunological interplay. Certain diseases stimulate PBMCs and contribute towards modulation of their proteome which can be effectively identified and evaluated via the comparative proteomics approach.Experimental design: In this review, we made a detailed survey of the PBMCS-derived protein biomarker candidates for a variety of diseases, published in the last 15 years. Articles were preselected to include only comparative proteomics studies.Results: PBMC-derived biomarkers were investigated for cancer, glomerular, neurodegenerative/neurodevelopmental, psychiatric, chronic inflammatory, autoimmune, endocrinal, infectious, and other diseases. A detailed review of these studies encompassed the proteomics platforms, proposed candidate biomarkers, their immune cell type specificity, and potential clinical application.Conclusions: Overall, PBMCs have shown a solid potential in giving early diagnostic and prognostic biomarkers for many diseases. The future of PBMC biomarker research should reveal its full potential through well-designed comparative studies and extensive testing of the most promising protein biomarkers identified so far.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":" ","pages":"e2300072"},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71485207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative comparison of the renal pelvic urine and bladder urine to examine modifications of the urine proteome by the lower urinary tract. 定量比较肾盂尿和膀胱尿，研究下尿路对尿液蛋白质组的改变。

IF 2.5 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-01 Epub Date: 2023-08-13 DOI: 10.1002/prca.202300004

Yilin Pan, Christine Yim-Ping Wong, Haiying Ma, Ryan Tsz-Hei Tse, Carol Ka-Lo Cheng, Miaomiao Tan, Peter Ka-Fung Chiu, Jeremy Yuen-Chun Teoh, Xin Wang, Chi-Fai Ng, Liang Zhang

{"title":"Quantitative comparison of the renal pelvic urine and bladder urine to examine modifications of the urine proteome by the lower urinary tract.","authors":"Yilin Pan, Christine Yim-Ping Wong, Haiying Ma, Ryan Tsz-Hei Tse, Carol Ka-Lo Cheng, Miaomiao Tan, Peter Ka-Fung Chiu, Jeremy Yuen-Chun Teoh, Xin Wang, Chi-Fai Ng, Liang Zhang","doi":"10.1002/prca.202300004","DOIUrl":"10.1002/prca.202300004","url":null,"abstract":"Purpose: Urine proteome is a valuable reservoir of biomarkers for disease diagnosis and monitoring. Following formation as the plasma filtrate in the kidney, urine is progressively modified by the active reabsorption and secretion of the urinary tract. However, little is known about how the urine proteome changes as it passes along the urinary tract.Experimental design: To investigate this, we compared the proteome composition of the renal pelvis urine (RPU) and individually self-voided bladder urine (BU) collected from seven unilateral urinary tract obstruction male patients by LC-MS/MS screening. To our knowledge, this is the first proteomic comparison of RPU and BU samples from the same individual.Results: Overall, RPU and BU proteomes did not exhibit proteins that were exclusively present in all samples of one urine type while in none of the other type. Nonetheless, BU had more overrepresented proteins that were observed at a higher frequency than RPU. Label-free quantitative analyses revealed BU-RPU differential proteins that are enriched in exosomes and extracellular proteins. However, the differences were not significant after corrections for multiple testing. Interestingly, we observed a significant increase of collagen peptides with hydroxyproline modifications in the BU samples, suggesting differences in protein modifications.Conclusions and clinical relevance: Our study revealed no substantial differences at the protein level between the BU and RPU samples. Future investigations with expanded cohorts would provide more insights about the urothelial-urinary interactions.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":" ","pages":"e2300004"},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9990697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parallel accumulation-serial fragmentation method for in-depth proteomic analysis of bronchoalveolar lavage fluid collected from patients with nonsmall cell lung cancer. 采用平行累积-序列片段分析法对非小细胞肺癌患者支气管肺泡灌洗液进行深入的蛋白质组学分析。

IF 2.5 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-01 Epub Date: 2024-01-31 DOI: 10.1002/prca.202300053

Hung M Vu, Sunghyun Huh, Jun Hyung Lee, Seung Hyeun Lee, Min-Sik Kim

{"title":"Parallel accumulation-serial fragmentation method for in-depth proteomic analysis of bronchoalveolar lavage fluid collected from patients with nonsmall cell lung cancer.","authors":"Hung M Vu, Sunghyun Huh, Jun Hyung Lee, Seung Hyeun Lee, Min-Sik Kim","doi":"10.1002/prca.202300053","DOIUrl":"10.1002/prca.202300053","url":null,"abstract":"Purpose: Advances in mass spectrometry-based quantitative proteomic analysis have successfully demonstrated the in-depth detection of protein biomarkers in bronchoalveolar lavage fluid (BALF) from patients with lung cancers. Recently, ion mobility technology was incorporated into the mass spectrometers escalating the sensitivity and throughput. Utilizing these advantages, herein, we employed the parallel accumulation-serial fragmentation (PASEF) implanted in a timsTOF Pro mass spectrometer to examine the alteration of BALF proteomes in patients with nonsmall cell lung cancers (NSCLCs).Experimental design: BALF proteins were processed from patients with NSCLC and analyzed in a timsTOF Pro mass spectrometer with the PASEF method using a peptide input of 100 ng. Label-free mass spectrometry data were analyzed in the FragPipe platform.Results: We quantitated over 1400 proteins from a single injection of 100 ng of peptides per sample with a median of ∼2000 proteins. We were able to find a few potential biomarker proteins upregulated in NSCLC.Conclusions and clinical relevance: The alterations of the BALF proteome landscape vary among patients with NSCLC as previously observed in patients with small-cell lung cancers. The PASEF method has significantly enhanced the sensitivity and throughput, demonstrating its effectiveness in clinical research and application.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":" ","pages":"e2300053"},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139651548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic landscape of primary and metastatic brain tumors for heterogeneity discovery. 用于异质性发现的原发性和转移性脑肿瘤的蛋白质组学景观。

IF 2.5 4区生物学

PROTEOMICS – Clinical Applications Pub Date : 2024-03-01 Epub Date: 2023-09-19 DOI: 10.1002/prca.202300010

Shuang Yang, Chengbin Zhou, Lei Zhang, Yueting Xiong, Yongtao Zheng, Liuguan Bian, Xiaohui Liu

{"title":"Proteomic landscape of primary and metastatic brain tumors for heterogeneity discovery.","authors":"Shuang Yang, Chengbin Zhou, Lei Zhang, Yueting Xiong, Yongtao Zheng, Liuguan Bian, Xiaohui Liu","doi":"10.1002/prca.202300010","DOIUrl":"10.1002/prca.202300010","url":null,"abstract":"Purpose: Despite recent advancements in our understanding of driver gene mutations and heterogeneity within brain tumors, whether primary or metastatic (also known as secondary), our comprehension of proteomic changes remains inadequate. The aim of this study is to provide an informative source for brain tumor researches, and distinguish primary brain tumors and secondary brain tumors from extracranial origins based on proteomic analysis.Experimental design: We assembled the most frequent brain tumors as follows: gliomas from WHO grade 2 to 4, with IDH1 mutations and wildtypes; brain metastases (BrMs) originating from lung cancer (LC), breast cancer (BC), ovarian cancer (OC), and colorectal cancer (CC). A total of 29 tissue samples were analyzed by label free quantitative mass spectrometry-based proteomics.Results: In total, 8165 protein groups were quantified, of which 4383 proteins were filtered at 50% valid intensity values for downstream analysis. Proteomic analysis of BrMs reveals conserved features shared among multiple origins. While proteomic heterogeneities were found for discriminating different grades of gliomas, as well as IDH1 mutant and wildtype gliomas. In addition, notable distinctions were observed at the pathway level between BrMs and gliomas. Specifically, BrMs exhibited characteristic pathways focused on proliferation and immunomodulation after colonizing the brain, whereas gliomas primarily engaged in invasion processes.Conclusions and clinical relevance: We characterized an extensive proteomic landscape of BrMs and gliomas. These findings have promising implications for the development of targeted therapies for BrMs and gliomas.","PeriodicalId":20571,"journal":{"name":"PROTEOMICS – Clinical Applications","volume":" ","pages":"e2300010"},"PeriodicalIF":2.5,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41150294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0