Proceedings of the National Academy of Sciences of the United States of America最新文献_第3页

Climbing the scala energiae: The cost of growing animals great and small.

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-24 Epub Date: 2024-12-16 DOI: 10.1073/pnas.2422898121

Eörs Szathmáry

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Eusocial workers must evolve before maternal control.

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-24 Epub Date: 2024-12-18 DOI: 10.1073/pnas.2422269121

Jack da Silva

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Reply to da Silva: Helping behavior can emerge without its prior adaptive evolution.

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-24 Epub Date: 2024-12-18 DOI: 10.1073/pnas.2423017121

Ella Rees-Baylis, Ido Pen, Jan J Kreider

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The danger of flipping an outside lipid to the inside.

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-24 Epub Date: 2024-12-16 DOI: 10.1073/pnas.2421371121

Todd R Graham

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Finding water on the Moon and Mars: Humanity's extraterrestrial future. 在月球和火星上找水：人类的地外未来。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-24 Epub Date: 2024-12-16 DOI: 10.1073/pnas.2421996121

Mark H Thiemens, Andreas Pack, Zachary Sharp

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Correction for Rockey et al., Ventilation does not affect close-range transmission of influenza virus in a ferret playpen setup.

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-24 Epub Date: 2024-12-16 DOI: 10.1073/pnas.2424158121

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Epithelial tubule interconnection driven by HGF-Met signaling in the kidney

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-20 DOI: 10.1073/pnas.2416887121

Isabel López-García, Sunhee Oh, Christopher Chaney, Jun Tsunezumi, Iain Drummond, Leif Oxburgh, Thomas J. Carroll, Denise K. Marciano

{"title":"Epithelial tubule interconnection driven by HGF-Met signaling in the kidney","authors":"Isabel López-García, Sunhee Oh, Christopher Chaney, Jun Tsunezumi, Iain Drummond, Leif Oxburgh, Thomas J. Carroll, Denise K. Marciano","doi":"10.1073/pnas.2416887121","DOIUrl":"https://doi.org/10.1073/pnas.2416887121","url":null,"abstract":"The formation of functional epithelial tubules is critical for the development and maintenance of many organ systems. While the mechanisms of tubule formation by epithelial cells are well studied, the process of tubule anastomosis—where tubules connect to form a continuous network—remains poorly understood. In this study, we utilized single-cell RNA sequencing to analyze embryonic mouse kidney tubules undergoing anastomosis. Our analysis identified hepatocyte growth factor (HGF) as a key potential mediator of this process. To investigate this further, we developed an assay using epithelial spheroids with fluorescently tagged apical surfaces, allowing us to visualize and quantify tubule–tubule connections. Our results demonstrate that HGF promotes tubule anastomosis, and it does so through the MAPK signaling pathway and MMPs, independently of cell proliferation. Remarkably, treatment with HGF and collagenase was sufficient to induce tubule anastomosis in embryonic mouse kidneys. These findings provide a foundational understanding of how to enhance the formation of functional tubular networks. This has significant clinical implications for the use of in vitro–grown kidney tissues in transplant medicine, potentially improving the success and integration of transplanted tissues.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"77 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Little evidence that nonmonogamous family structures are detrimental to children’s well-being in Mpimbwe, Tanzania

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-20 DOI: 10.1073/pnas.2407785121

Riana Minocher, Monique Borgerhoff Mulder, Cody T. Ross

{"title":"Little evidence that nonmonogamous family structures are detrimental to children’s well-being in Mpimbwe, Tanzania","authors":"Riana Minocher, Monique Borgerhoff Mulder, Cody T. Ross","doi":"10.1073/pnas.2407785121","DOIUrl":"https://doi.org/10.1073/pnas.2407785121","url":null,"abstract":"Nuclear family structures are often thought to be essential for the well-being of children. Divorce, the loss of either biological parent, the presence of step-parents, and the practice of polygynous marriage have all been claimed to negatively impact child well-being. However, empirical research on these topics has been limited by the routine use of cross-regional and cross-sectional databases. Cross-regional data render research vulnerable to the ecological inference fallacy, and cross-sectional data prevent assessment of age-specific impacts of time-varying family-structure variables. When longitudinal data are available, they tend to be drawn from Western/urban contexts. Detailed data on family structure and children’s well-being are rarely collected in more marginalized communities. In many rural and traditional communities, nonnuclear family structures are indeed prevalent and viewed as socially permissible—and, as such, may have different impacts on children’s well-being than in Western contexts. Here, we draw on a detailed, longitudinal dataset from a 20-y prospective study in rural Tanzania, where polygyny and serial monogamy are common. We analyze survival outcomes for 3,693 children born between 1931 and 2014, growth outcomes for 881 children born between 1976 and 2014, and educational outcomes for 1,370 children born between 1976 and 2014. Our analyses indicate that monogamous marriage is not consistently associated with better outcomes for children—contrary to some popular and public health perspectives on human family structure.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"13 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Docetaxel response in BRCA1,p53-deficient mammary tumor cells is affected by Huntingtin and BAP1

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-20 DOI: 10.1073/pnas.2402849121

Martín González-Fernández, Carmen Perry, Nora Merete Gerhards, Paola Francica, Sven Rottenberg

{"title":"Docetaxel response in BRCA1,p53-deficient mammary tumor cells is affected by Huntingtin and BAP1","authors":"Martín González-Fernández, Carmen Perry, Nora Merete Gerhards, Paola Francica, Sven Rottenberg","doi":"10.1073/pnas.2402849121","DOIUrl":"https://doi.org/10.1073/pnas.2402849121","url":null,"abstract":"Taxanes are frequently used anticancer drugs known to kill tumor cells by inducing mitotic aberrations and segregation defects. A defining feature of specific cancers, notably triple-negative breast cancer (TNBC) and particularly those deficient in BRCA1, is chromosomal instability (CIN). Here, we focused on understanding the mechanisms of docetaxel-induced cytotoxicity, especially in the context of BRCA1-deficient TNBC. Using functional genetic screens in CIN+ cells, we identified genes that mediate docetaxel response and found an interaction between Huntingtin (HTT) and BRCA1-associated protein-1 (BAP1). We employed Brca1 −/− ; p53 − /− mammary tumor cells, derived from genetically engineered mouse tumors that closely mimic the human disease, to investigate the role of these genes in CIN+ BRCA1-deficient cells. Specifically, we observed that loss of HTT sensitizes CIN+ BRCA1-deficient mammary tumor cells to docetaxel by shortening mitotic spindle poles and increasing spindle multipolarity. In contrast, BAP1 depletion protected cells against these spindle aberrations by restoring spindle length and enhancing mitotic clustering of the extra centrosomes. In conclusion, our findings shed light on the roles of HTT and BAP1 in controlling mitotic spindle multipolarity and centrosome clustering, specifically in the absence of BRCA1. This affects the response to microtubule-targeting agents and suggests that further studies of the interaction of these genes with the mitotic spindle may provide useful insights into how to target CIN+ cells, particularly in the challenging therapeutic landscape of BRCA1-deficient TNBC.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"11 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zscan4 mediates ubiquitination and degradation of the corepressor complex to promote chromatin accessibility in 2C-like cells

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2024-12-20 DOI: 10.1073/pnas.2407490121

Jiao Yang, Jiameng Dan, Nannan Zhao, Linlin Liu, Huasong Wang, Qiangqiang Liu, Lingling Wang, Jie Li, Yiwei Wu, Feilong Chen, Weilun Fu, Fei Liu, Meiqi Lin, Weiyu Zhang, Fuquan Chen, Xinqi Liu, Xinyi Lu, Quan Chen, Xudong Wu, Yuyu Niu, Na Yang, Yushan Zhu, Jiafu Long, Lin Liu

{"title":"Zscan4 mediates ubiquitination and degradation of the corepressor complex to promote chromatin accessibility in 2C-like cells","authors":"Jiao Yang, Jiameng Dan, Nannan Zhao, Linlin Liu, Huasong Wang, Qiangqiang Liu, Lingling Wang, Jie Li, Yiwei Wu, Feilong Chen, Weilun Fu, Fei Liu, Meiqi Lin, Weiyu Zhang, Fuquan Chen, Xinqi Liu, Xinyi Lu, Quan Chen, Xudong Wu, Yuyu Niu, Na Yang, Yushan Zhu, Jiafu Long, Lin Liu","doi":"10.1073/pnas.2407490121","DOIUrl":"https://doi.org/10.1073/pnas.2407490121","url":null,"abstract":"Zygotic genome activation occurs in two-cell (2C) embryos, and a 2C-like state is also activated in sporadic (~1%) naïve embryonic stem cells in mice. Elevated chromatin accessibility is critical for the 2C-like state to occur, yet the underlying molecular mechanisms remain elusive. Zscan4 exhibits burst expression in 2C embryos and 2C-like cells. Here, we show that Zscan4 mediates chromatin remodeling to promote the chromatin accessibility for achieving the 2C-like state. Through coimmunoprecipitation/mass spectrometry, we identified that Zscan4 interacts with the corepressors Kap1/Trim28, Lsd1, and Hdac1, also with H3K9me3 modifiers Suv39h1/2, to transiently form a repressive chromatin complex. Then, Zscan4 mediates the degradation of these chromatin repressors by recruiting Trim25 as an E3 ligase, enabling the ubiquitination of Lsd1, Hdac1, and Suv39h1/2. Degradation of the chromatin repressors promotes the chromatin accessibility for activation of the 2C-like state. These findings reveal the molecular insights into the roles of Zscan4 in promoting full activation of the 2C-like state.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"269 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0