Proceedings of the National Academy of Sciences of the United States of America最新文献

IF 9.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-07 Epub Date: 2025-09-29 DOI: 10.1073/pnas.2522472122

Ankur R Desai

引用次数: 0

Correction for Gilboa et al., Measurement of α-synuclein as protein cargo in plasma extracellular vesicles. 修正Gilboa等人，血浆细胞外囊泡中α-突触核蛋白作为蛋白质货物的测量。

IF 9.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-07 Epub Date: 2025-10-01 DOI: 10.1073/pnas.2522569122

引用次数: 0

Hierarchical woven fibrillar structures in developing single gyroids in butterflies. 蝴蝶单陀螺发育中的分层编织纤维结构。

IF 9.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-07 Epub Date: 2025-09-26 DOI: 10.1073/pnas.2507297122

Anna-Lee Jessop, Peta L Clode, Martin Saunders, Myfanwy E Evans, Stephen T Hyde, James N McPherson, Kasper S Pedersen, Jacob J K Kirkensgaard, Nipam H Patel, Kyle A DeMarr, W Owen McMillan, Bodo D Wilts, Gerd E Schröder-Turk

{"title":"Hierarchical woven fibrillar structures in developing single gyroids in butterflies.","authors":"Anna-Lee Jessop, Peta L Clode, Martin Saunders, Myfanwy E Evans, Stephen T Hyde, James N McPherson, Kasper S Pedersen, Jacob J K Kirkensgaard, Nipam H Patel, Kyle A DeMarr, W Owen McMillan, Bodo D Wilts, Gerd E Schröder-Turk","doi":"10.1073/pnas.2507297122","DOIUrl":"10.1073/pnas.2507297122","url":null,"abstract":"Nature offers a remarkable diversity of nanomaterials that have extraordinary functional and structural properties. Intrinsic to nature is the impressive ability to form complex ordered nanomaterials via self-organization. One particularly intriguing nanostructure is the gyroid, a network-like structure exhibiting high symmetry and complex topology. Although its existence in cells and tissues across many biological kingdoms is well documented, how and why it forms remains elusive and uncovering these formation mechanisms will undoubtedly inform bioinspired designs. A beautiful example is the smooth single gyroid that is found in the wing scales of several butterflies, where it behaves as a photonic crystal generating a vibrant green color. Here, we report that the gyroid structures of the Emerald-patched Cattleheart, Parides sesostris, develop as woven fibrillar structures, in contrast to the commonly held assumption that they form as smooth constructs. Ultramicroscopy of pupal tissue reveals that the gyroid geometry consists of helical weavings of fibers, akin to hyperbolic line patterns decorating the gyroid. Interestingly, despite their fibrillar nature, electron diffraction reveals the absence of crystalline order within this material. Similar fibrillar structures are also observed in the mature wing scales of P. sesostris specimens with surgically altered pupal development, leading to a blue coloration. Our findings not only introduce a variation of the gyroid in biology but also have significant implications for our understanding of its formation in nature.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 40","pages":"e2507297122"},"PeriodicalIF":9.1,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chemical epigenetic tool to probe site-specific DNA-binding protein complexes. 一种化学表观遗传学工具，用于探测位点特异性dna结合蛋白复合物。

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2509021122

Jiajun Zhu,Zhucui Li,Dongxiang Xue,Zihe Meng,Sida Shao,Julian Pulecio,Guoan Zhang,Danwei Huangfu,Todd Evans,Shuibing Chen,Peter G Schultz

{"title":"A chemical epigenetic tool to probe site-specific DNA-binding protein complexes.","authors":"Jiajun Zhu,Zhucui Li,Dongxiang Xue,Zihe Meng,Sida Shao,Julian Pulecio,Guoan Zhang,Danwei Huangfu,Todd Evans,Shuibing Chen,Peter G Schultz","doi":"10.1073/pnas.2509021122","DOIUrl":"https://doi.org/10.1073/pnas.2509021122","url":null,"abstract":"Site-specific DNA binding by proteins is critical for regulating transcriptional activity and cell fate decision. However, identifying proteins bound to specific genomic regions (e.g., promoter or enhancer regions) remains challenging. To address this, we developed a chemical epigenetic tool, named Site-specific noncanonical amino acid-mediated capture of protein (SCOPE), incorporating a photo-crosslinking amino acid into a nuclease-deficient dCas9 mutant. Human pluripotent stem cells (hPSCs) carrying SCOPE enable the capture of proteins bound to, in theory, any genomic location, facilitating the study of the cell context-dependent DNA-protein interactions. Using SCOPE, we identified the OCT4/SOX2/CARHSP1 complex binding to the NANOG promoter to maintain pluripotency in hPSCs. During ectoderm differentiation, ZIC2 acts as a competitive inhibitor, binding the same promoter region to downregulate NANOG expression and promote differentiation. Additionally, SCOPE identified that ZNF8 binds to the distal regulatory region of OCT4 to maintain naïve pluripotency. In summary, SCOPE provides a robust system for uncovering cell context-dependent, site-specific genome regulators, offering valuable insights into gene regulation networks driving cell fate transitions.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"78 1","pages":"e2509021122"},"PeriodicalIF":11.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geometrical compartmentalization of trigger waves 触发波的几何分隔

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2512872122

Zhengda Li, Kevin S. Zhang, Yuping Chen, Sindy K. Y. Tang, James E. Ferrell

{"title":"Geometrical compartmentalization of trigger waves","authors":"Zhengda Li, Kevin S. Zhang, Yuping Chen, Sindy K. Y. Tang, James E. Ferrell","doi":"10.1073/pnas.2512872122","DOIUrl":"https://doi.org/10.1073/pnas.2512872122","url":null,"abstract":"Trigger waves, self-regenerating fronts of biochemical activity that spread without losing speed or amplitude, are widespread in cell signaling. Apoptosis is one example of a process that propagates through the cytoplasm via trigger waves. Curiously, in some contexts, like synaptic pruning, apoptotic caspase activation is confined to specific subcellular regions. We hypothesized that at junctions between a thin cytoplasmic extension, like a dendritic spine, and a thicker one, like a dendrite, trigger wave propagation may be blocked even though diffusion is not, as a result of the general properties of trigger waves and bistable systems. This hypothesis was explored theoretically through modeling studies and dimensional arguments, which confirmed that trigger wave compartmentalization was possible and that the critical channel width required for compartmentalization was likely to be biologically relevant. These predictions were then tested experimentally with undiluted Xenopus egg extracts that were induced to undergo apoptosis. We found that channels that are less than a few microns in diameter are small enough to compartmentalize apoptosis, and that the critical width is inversely proportional to trigger wave speed. Thus, cellular projections and tubules can allow for the compartmentalization of biochemical states within a spatially continuous cytoplasm, a fundamental yet previously overlooked mechanism for controlling biochemical signaling and cellular functions.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"21 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced PIEZO1 function contributes to the pathogenesis of sickle cell disease PIEZO1功能的增强参与镰状细胞病的发病机制

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2514863122

Luis O. Romero, Manisha Bade, Laila Elsherif, Jada D. Williams, Xiangmei Kong, Adebowale Adebiyi, Kenneth I. Ataga, Shang Ma, Julio F. Cordero-Morales, Valeria Vásquez

{"title":"Enhanced PIEZO1 function contributes to the pathogenesis of sickle cell disease","authors":"Luis O. Romero, Manisha Bade, Laila Elsherif, Jada D. Williams, Xiangmei Kong, Adebowale Adebiyi, Kenneth I. Ataga, Shang Ma, Julio F. Cordero-Morales, Valeria Vásquez","doi":"10.1073/pnas.2514863122","DOIUrl":"https://doi.org/10.1073/pnas.2514863122","url":null,"abstract":"Sickle cell disease (SCD), an inherited blood disorder caused by a mutation in the β-globin gene, is characterized by sickle erythrocytes that are prone to hemolysis, leading to anemia and vaso-occlusion crises. In sickle erythrocytes, hemoglobin aggregation is followed by altered cation permeability and subsequent dehydration. Interventions that restore cation permeability can decrease hemolysis and ameliorate the symptoms associated with SCD. PIEZO1 is a nonselective mechanosensitive cation channel that regulates erythrocyte volume. Gain-of-function (GOF) mutations in PIEZO1 cause hemolytic anemia by increasing cation permeability, leading to erythrocyte dehydration in humans and mice. Although PIEZO1 plays a key role in erythrocyte homeostasis, its role in SCD remains unknown. Here, we demonstrate that the function of the PIEZO1 channel is upregulated in sickle erythrocytes of humans and mice, and this enhancement can be restored through a dietary intervention. We found that PIEZO1 activity in sickle erythrocytes resembles that of the GOF mutation causing hemolytic anemia. A diet enriched in the ω -3 fatty acid eicosapentaenoic (EPA) acid decreases PIEZO1 activity in sickle erythrocytes, attenuates hemolysis, and reduces hypoxia-induced sickling. Furthermore, EPA reduces inflammatory markers. We propose that PIEZO1 contributes to the increase in nonselective cationic conductance (i.e., Psickle), which leads to dehydration downstream of hemoglobin polymerization. Our results suggest that reducing PIEZO1 function represents a promising therapeutic strategy to reestablishing normal cation permeability in SCD.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"11 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soluble guanylyl cyclase, the NO receptor, drives vasorelaxation via endothelial S-nitrosation. 可溶性胍基环化酶，一氧化氮受体，通过内皮s -亚硝化驱动血管松弛。

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2519105122

Waqas Younis,Pia C Burboa,Tanaz Sadeghian,Veronica Kuzdowicz,Chuanlong Cui,Ping Shu,Yong Qin,Lai-Hua Xie,Mauricio A Lillo,Annie Beuve

{"title":"Soluble guanylyl cyclase, the NO receptor, drives vasorelaxation via endothelial S-nitrosation.","authors":"Waqas Younis,Pia C Burboa,Tanaz Sadeghian,Veronica Kuzdowicz,Chuanlong Cui,Ping Shu,Yong Qin,Lai-Hua Xie,Mauricio A Lillo,Annie Beuve","doi":"10.1073/pnas.2519105122","DOIUrl":"https://doi.org/10.1073/pnas.2519105122","url":null,"abstract":"We previously demonstrated that the NO-stimulated soluble guanylyl cyclase (GC1), which produces cGMP, also has the ability to transfer S-nitrosothiols (SNO) to other proteins in a reaction involving oxidized Thioredoxin 1 (oTrx1). This transnitrosation cascade was established in vitro and involved Cys 610 (C610) of GC1 as the major SNO-donor. To assay the relevance of GC1 transnitrosation under physiological conditions and in oxidative pathologies, we studied a knock-in mouse in which C610 was replaced with a serine (KI αC610S) under basal or angiotensin II (Ang II)-treated conditions. Despite similar GC1 expression and NO responsiveness, the Ang II-treated KI mice displayed exacerbated oxidative pathologies including higher mean arterial pressure and more severe cardiac dysfunctions compared to the Ang II-treated WT. These phenotypes were associated with a drastic decrease in global S-nitrosation and in levels of SNO-Trx1 in the KI mice. To investigate the mechanism underlying the dysregulation of blood pressure, pressure myography and in vivo intravital microscopy were conducted to analyze the vascular tone of resistance vessels. Both approaches indicated that, even in the absence of oxidative stress, the single mutation C610S led to a significant disruption of the endothelium-dependent, acetylcholine-induced vasorelaxation while NO-dependent smooth muscle relaxation remained unchanged. Mechanistically, the vasorelaxation defect was associated with decreased endothelial calcium influx and membrane hyperpolarization, independent of NO bioavailability. These findings indicate that the C610S mutation uncouples two NO signaling vasodilatory pathways (endothelial SNO and smooth muscle NO-cGMP) and suggest that GC1 transnitrosation activity is essential for endothelium-derived hyperpolarization.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"28 1","pages":"e2519105122"},"PeriodicalIF":11.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Density-dependent recruitment but not survival drives cyclic dynamics in a field vole population. 在田鼠种群中，依赖于密度的招募而不是生存驱动着循环动力学。

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2509516122

Xavier Lambin,Mike Begon,Sarah J Burthe,Isla M Graham,James L MacKinnon,Sandra Telfer,Madan K Oli

{"title":"Density-dependent recruitment but not survival drives cyclic dynamics in a field vole population.","authors":"Xavier Lambin,Mike Begon,Sarah J Burthe,Isla M Graham,James L MacKinnon,Sandra Telfer,Madan K Oli","doi":"10.1073/pnas.2509516122","DOIUrl":"https://doi.org/10.1073/pnas.2509516122","url":null,"abstract":"Arguably, the most fundamental question in population ecology is what drives patterns in the abundance of populations? Small rodents exhibiting regular multiannual cycles in abundance have long been a test bed for addressing this question. The prevailing orthodoxy, the predation hypothesis, contends that nonmigratory, specialist predators are necessary, and specialist and generalist predators, combined, are both necessary and sufficient, for causing population cycles. Thus, variations in survival, from predation, are the key drivers of the cycles. However, this, and other competing theories, have hitherto lacked supportive demographic evidence and hence a solid evidential foundation. Here, we provide such evidence, analyzing 10 y of monthly data from a cyclic field vole population. We find, contrary to the prevailing orthodoxy, that recruitment, not survival, varied substantially from phase to phase in the cycles, made the major contribution to variations in population growth rate, and had cycle-phase-specific negative delayed density dependence. These results, their consistency with what is known from other systems, and the weak demographic foundations of the predation hypothesis, together suggest recruitment, specifically breeding-season length, not predation, as the cycles' driving force. They therefore suggest that re-evaluation of the importance of the various determinants of population abundances, more generally, may be necessary.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"32 1","pages":"e2509516122"},"PeriodicalIF":11.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From propulsion to suction: Unraveling thrust reversal in propellers at intermediate Reynolds numbers. 从推进到吸力：在中间雷诺数下螺旋桨的推力反转。

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2504153122

Rong Fu,Siyu Li,Yang Ding

引用次数: 0

Chemobiological synthesis of benzene, toluene, ethylbenzene, and xylene from glucose or glycerol. 用葡萄糖或甘油合成苯、甲苯、乙苯和二甲苯的化学生物学方法。

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-10-02 DOI: 10.1073/pnas.2509568122

Xuan Zou,Taewan Kim,Zi Wei Luo,Kyeong Rok Choi,Sunkyu Han,Sang Yup Lee

{"title":"Chemobiological synthesis of benzene, toluene, ethylbenzene, and xylene from glucose or glycerol.","authors":"Xuan Zou,Taewan Kim,Zi Wei Luo,Kyeong Rok Choi,Sunkyu Han,Sang Yup Lee","doi":"10.1073/pnas.2509568122","DOIUrl":"https://doi.org/10.1073/pnas.2509568122","url":null,"abstract":"Benzene, toluene, ethylbenzene, and p-xylene (BTEX) are key aromatic hydrocarbons widely used in fuels, polymers, and industrial chemicals, yet their production remains heavily dependent on fossil resources, raising environmental and public health concerns. To promote de novo production of BTEX from renewable feedstocks, we developed a chemobiological platform that integrates microbial biosynthesis with chemical deoxygenation. Four metabolically engineered Escherichia coli strains were constructed to produce one of four oxygenated precursors of BTEX-phenol, benzyl alcohol, 2-phenylethanol, or 2,5-xylenol-from glucose or glycerol. After in situ two-phase extractive fermentation of the individual engineered strains using isopropyl myristate (IPM) as the organic solvent, the organic phase containing one of the oxygenated precursors was separated from the aqueous phase and subjected to distinct chemical deoxygenation reactions to reduce the precursor to the corresponding BTEX compound. This modular approach, based on the compatible organic solvent, streamlines biotransformation and consecutive chemical derivatization, providing a practical and viable route to sustainable BTEX production. The platform is extensible and provides a generalizable framework for integrating biosynthesis with chemical deoxygenation in hybrid bioprocessing.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"506 1","pages":"e2509568122"},"PeriodicalIF":11.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0