Proceedings of the National Academy of Sciences of the United States of America最新文献

Correction for Ahituv et al., Starch-rich plant foods 780,000 y ago: Evidence from Acheulian percussive stone tools. 对Ahituv等人的更正，78万年前富含淀粉的植物性食物：来自阿舍利敲击石器的证据。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-16 DOI: 10.1073/pnas.2508254122

引用次数: 0

Retraction for Austin et al., Death-receptor activation halts clathrin-dependent endocytosis. 对于Austin等人来说，死亡受体的激活阻止了网格蛋白依赖的内吞作用。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-15 DOI: 10.1073/pnas.2510913122

引用次数: 0

Structure and evolution of photosystem I in the early-branching cyanobacterium Anthocerotibacter panamensis. 早分枝蓝藻巴拿马Anthocerotibacter panamensis光系统I的结构和进化。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-14 DOI: 10.1073/pnas.2427090122

Han-Wei Jiang, Christopher J Gisriel, Tanai Cardona, David A Flesher, Gary W Brudvig, Ming-Yang Ho

{"title":"Structure and evolution of photosystem I in the early-branching cyanobacterium Anthocerotibacter panamensis.","authors":"Han-Wei Jiang, Christopher J Gisriel, Tanai Cardona, David A Flesher, Gary W Brudvig, Ming-Yang Ho","doi":"10.1073/pnas.2427090122","DOIUrl":"10.1073/pnas.2427090122","url":null,"abstract":"Thylakoid-free cyanobacteria are thought to preserve ancestral traits of early-evolving organisms capable of oxygenic photosynthesis. However, and until recently, photosynthesis studies in thylakoid-free cyanobacteria were only possible in the model strain Gloeobacter violaceus, limiting our understanding of photosynthesis evolution. Here, we report the isolation, biochemical characterization, cryo-EM structure, and phylogenetic analysis of photosystem I (PSI) from a recently discovered thylakoid-free cyanobacterium, Anthocerotibacter panamensis, a distant relative of the genus Gloeobacter. We find that A. panamensis PSI exhibits a distinct carotenoid composition and has one conserved low-energy chlorophyll site, which was lost in G. violaceus. Furthermore, PSI in thylakoid-free cyanobacteria has changed at the sequence level to a degree comparable to that of other strains, yet its subunit composition and oligomeric form might be identical to that of the most recent common ancestor of cyanobacteria. This study therefore provides a glimpse into the ancient evolution of photosynthesis.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 20","pages":"e2427090122"},"PeriodicalIF":9.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural basis of human Na_v1.5 gating mechanisms. 人类Nav1.5门控机制的结构基础。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-14 DOI: 10.1073/pnas.2416181122

Rupam Biswas, Ana Laura López-Serrano, Apoorva Purohit, Angelina Ramirez-Navarro, Hsiang-Ling Huang, Giovanna Grandinetti, Xiaolin Cheng, Sarah M Heissler, Isabelle Deschênes, Krishna Chinthalapudi

{"title":"Structural basis of human Nav1.5 gating mechanisms.","authors":"Rupam Biswas, Ana Laura López-Serrano, Apoorva Purohit, Angelina Ramirez-Navarro, Hsiang-Ling Huang, Giovanna Grandinetti, Xiaolin Cheng, Sarah M Heissler, Isabelle Deschênes, Krishna Chinthalapudi","doi":"10.1073/pnas.2416181122","DOIUrl":"10.1073/pnas.2416181122","url":null,"abstract":"Voltage-gated Nav1.5 channels are central to the generation and propagation of cardiac action potentials. Aberrations in their function are associated with a wide spectrum of cardiac diseases including arrhythmias and heart failure. Despite decades of progress in Nav1.5 biology, the lack of structural insights into intracellular regions has hampered our understanding of its gating mechanisms. Here, we present two cryo-EM structures of human Nav1.5 in open states, revealing sequential conformational changes in gating charges of the voltage-sensing domains (VSDs) and several intracellular regions. Despite the channel being in the open state, these structures show repositioning, but no dislodging of the IFM motif in the receptor site. Molecular dynamics analyses show our structures with CTD conduct Na+ ions. Notably, our structural findings highlight a dynamic C-terminal domain (CTD) and III-IV linker interaction, which regulates the conformation of VSDs and pore opening. Electrophysiological studies confirm that disrupting this interaction alters fast inactivation of Nav1.5. Together, our structure-function studies establish a foundation for understanding the gating mechanisms of Nav1.5 and the mechanisms underlying CTD-related channelopathies.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 20","pages":"e2416181122"},"PeriodicalIF":9.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kernels of selfhood: GPT-4o shows humanlike patterns of cognitive dissonance moderated by free choice. 自我的核心：gpt - 40显示了由自由选择调节的人类认知失调模式。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-14 DOI: 10.1073/pnas.2501823122

Steven A Lehr, Ketan S Saichandran, Eddie Harmon-Jones, Nykko Vitali, Mahzarin R Banaji

引用次数: 0

Exact and efficient phylodynamic simulation from arbitrarily large populations. 从任意大的种群精确和有效的系统动力学模拟。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-14 DOI: 10.1073/pnas.2412978122

Michael Celentano, William S DeWitt, Sebastian Prillo, Yun S Song

{"title":"Exact and efficient phylodynamic simulation from arbitrarily large populations.","authors":"Michael Celentano, William S DeWitt, Sebastian Prillo, Yun S Song","doi":"10.1073/pnas.2412978122","DOIUrl":"10.1073/pnas.2412978122","url":null,"abstract":"Many biological studies involve inferring the evolutionary history of a sample of individuals from a large population and interpreting the reconstructed tree. Such an ascertained tree typically represents only a small part of a comprehensive population tree and is distorted by survivorship and sampling biases. Inferring evolutionary parameters from ascertained trees requires modeling both the underlying population dynamics and the ascertainment process. A crucial component of this phylodynamic modeling involves tree simulation, which is used to benchmark probabilistic inference methods. To simulate an ascertained tree, one must first simulate the full population tree and then prune unobserved lineages. Consequently, the computational cost is determined not by the size of the final simulated tree, but by the size of the population tree in which it is embedded. In most biological scenarios, simulations of the entire population are prohibitively expensive due to computational demands placed on lineages without sampled descendants. Here, we address this challenge by proving that, for any partially ascertained process from a general multitype birth-death-mutation-sampling model, there exists an equivalent process with complete sampling and no death, a property which we leverage to develop a highly efficient algorithm for simulating trees. Our algorithm scales linearly with the size of the final simulated tree and is independent of the population size, enabling simulations from extremely large populations beyond the reach of current methods but essential for various biological applications. We anticipate that this massive speedup will significantly advance the development of novel inference methods that require extensive training data.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 20","pages":"e2412978122"},"PeriodicalIF":9.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lane formation in criss-crossing crowds. 纵横交错的人群中形成的小巷。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-12 DOI: 10.1073/pnas.2505488122

Klaus Kroy

引用次数: 0

Electric field-induced pore constriction in the human K_v2.1 channel. 电场诱导的人体Kv2.1通道孔隙收缩。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-14 DOI: 10.1073/pnas.2426744122

Venkata Shiva Mandala, Roderick MacKinnon

{"title":"Electric field-induced pore constriction in the human Kv2.1 channel.","authors":"Venkata Shiva Mandala, Roderick MacKinnon","doi":"10.1073/pnas.2426744122","DOIUrl":"10.1073/pnas.2426744122","url":null,"abstract":"Gating in voltage-dependent ion channels is regulated by the transmembrane voltage. This form of regulation is enabled by voltage-sensing domains (VSDs) that respond to transmembrane voltage differences by changing their conformation and exerting force on the pore to open or close it. Here, we use cryogenic electron microscopy to study the neuronal Kv2.1 channel in lipid vesicles with and without a voltage difference across the membrane. Hyperpolarizing voltage differences displace the positively charged S4 helix in the voltage sensor by one helical turn (~5 Å). When this displacement occurs, the S4 helix changes its contact with the pore at two different interfaces. When these changes are observed in fewer than four voltage sensors, the pore remains open, but when they are observed in all four voltage sensors, the pore constricts. The constriction occurs because the S4 helix, as it displaces inward, squeezes the right-handed helical bundle of pore-lining S6 helices. A similar conformational change occurs upon hyperpolarization of the EAG1 channel but with two helical turns displaced instead of one. Therefore, while Kv2.1 and EAG1 are from distinct architectural classes of voltage-dependent ion channels, called domain-swapped and non-domain-swapped, the way the voltage sensors gate their pores is very similar.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 20","pages":"e2426744122"},"PeriodicalIF":9.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QnAs with Thomas A. Henzinger. Thomas A. Henzinger的QnAs。

IF 9.4 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-20 Epub Date: 2025-05-14 DOI: 10.1073/pnas.2509901122

Sandeep Ravindran

引用次数: 0

Autocatalytic symmetry breaking and chiral amplification in a feedback network combining amino acid synthesis and ligation 结合氨基酸合成和连接的反馈网络中的自催化对称破缺和手性扩增

IF 11.1 1区综合性期刊

Proceedings of the National Academy of Sciences of the United States of America Pub Date : 2025-05-16 DOI: 10.1073/pnas.2423683122

Paul G. Higgs, Donna G. Blackmond

引用次数: 0