Problemy radiatsiinoi medytsyny ta radiobiolohii最新文献

{"title":"EFFECTIVENESS OF THE PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT HUMAN CELLS ІN A RESTING STATE.","authors":"G Y Lavrenchuk, V V Talko, A V Chernyshov","doi":"10.33145/2304-8336-2023-28-206-215","DOIUrl":"10.33145/2304-8336-2023-28-206-215","url":null,"abstract":"<p><strong>Objective: </strong>to investigate the structural and morphofunctional changes in test system of malignant (cell line A-549) human cells in a resting state exposed to X-rays in the presence of gadolinium-containing photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Photolon» photosensitizer.</p><p><strong>Methods: </strong>Passaged malignant human cell culture technology, X-ray and red light exposure, cytological and statistical methods.</p><p><strong>Results: </strong>X-ray exposure at a dose of 10.0 Gy in the presence of photon capture agent «Dotavist» (at a 100 μg/ml nutrient medium concentration) led to death of 75-83 % of malignant cells in a resting state on the 6-8th day of cultivation. Photodynamic exposure (630 nm wavelength red light) in the presence of «Photolon» photosensitizer (200 μg/ml concentration) resulted in death of 69-73 % of malignant cells, respectively. Combination of the photon-capturing technology and photodynamic exposure resulted in death of 90 % of the malignant cells in a phase of steady-state growth on the 8th day of cultivation.</p><p><strong>Conclusion: </strong>Combination of the photon capture technology (X-ray exposure with gadolinium-containing photoncapture agent «Dotavist» in cytotoxic concentration) and photodynamic exposure in the presence of «Photolon» photosensitizer increased devitalization effectiveness of human non-small cell lung cancer cells (A-549 cell line) being in a steady-state growth phase up to 90 %. Ten percent of cells resistant to the applied technologies retained their proliferative potential, evident as changes in their morphology, genotype and adhesiveness during further cultivation.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"206-215"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEMATOPOIETIC PROGENITORS CELLS IN PERIPHERAL BLOOD OF BALB/C MICE UNDER IONIZING RADIATION ACTION IN SUBLETHAL DOSE.","authors":"I Z Russu, D I Bilko, N M Bilko","doi":"10.33145/2304-8336-2023-28-216-224","DOIUrl":"10.33145/2304-8336-2023-28-216-224","url":null,"abstract":"<p><strong>Objective: </strong>determination of the content of hematopoietic progenitor cells circulating in peripheral blood of Balb/Cmice, under ionizing radiation action in sublethal dose, at different periods after the irradiation, using cell culturein diffusion chambers in vivo.</p><p><strong>Methods: </strong>Peripheral blood smears of Balb/C mice were prepared and studied, its cellular composition was determined, as well as by cultivation of peripheral blood cells in diffusion chambers in vivo their colony-forming efficien-cy was determined on the 0th, 5th, and 30th day after external irradiation in sublethal dose 5.85 Gy.</p><p><strong>Results: </strong>The content of myelocytes and metamyelocytes among blood nucleated cells of the irradiated animals wasincreased, compared to control, during the whole investigated period. In particular, on the 30th day after irradiationthe content of myelocytes in peripheral blood was 3.3 ± 0.7 % compared to (0.8 ± 0.4) % in control, and the content of metamyelocytes - (3.4 ± 0.7) % compared to (0.9 ± 0.3) % in control. A significant increase in the amountof circulating progenitor cells in the peripheral blood was observed in the early stages after irradiation (12.5 ± 1.6colony-forming units per 100,000 explanted cells, compared to 5.1 ± 0.8 in control). However, on the 5th day theircontent was slightly reduced compared to control (1.3 ± 0.9), and only to the 30th day a normalization of the amountof progenitor cells occurred in the peripheral blood (6.8 ± 0.7 colony-forming units per 100,000 explanted cells).</p><p><strong>Conclusions: </strong>The analysis of the obtained results revealed an increased level of immature forms of cells in theperipheral blood of irradiated animals, compared to control, in the early stages after irradiation, includinghematopoietic progenitor cells, which are able to colony forming in cell culture. Therefore, the action of ionizingradiation in sublethal dose had a critical effect on the proliferation of hematopoietic cells in bone marrow and provoked their increased migration into the bloodstream. Determination of the content of hematopoietic cells' immature forms in peripheral blood allowed assessing the degree of hematopoietic damage due to the action of ionizing radiation.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"216-224"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STRUCTURE AND CHARACTERISTICS OF THE CHORNOBYL NPP ACCIDENT VICTIMS ACCORDING TO THE MATERIALS OF MEDICAL EXPERTISE OF THE CAUSAL RELATIONSHIP OF DISEASES WITH THE EFFECT OF IONIZING RADIATION IN THE REMOTE POSTACCIDENTAL PERIOD (2023 year).","authors":"V O Sushko, O O Kolosynska","doi":"10.33145/2304-8336-2023-28-424-430","DOIUrl":"10.33145/2304-8336-2023-28-424-430","url":null,"abstract":"<p><p>Loss of health and workability, as well as deaths due to harmful influence of the Chornobyl catastrophe (including radiation exposure) during performance of professional, military or official duties and / or living on radiation-contaminated areas, additional exposure not through their own fault but due to a radiation accident, caused the development of a special form of medical expertise as part of the of medical social protection system for these suffered contingents.</p><p><strong>Objective: </strong>To study and characterize the structure of the affected categories (clean-up workers and victims) of the Chornobyl catastrophe based on the materials of expert cases of the Central Interdepartmental Expert Commission of Ministry of Health of Ukraine (CIEC) in the remote post-accidental period (2013-2023).</p><p><strong>Material and methods: </strong>The work was performed in the design of a retrospective study that based on analysis of the structure of all categories of Chornobyl NPP accident (ChNPP) victims during 2008-2023 years and studying of more 57 000 medical expert cases, which were considered by CIEC during 2013-2022 to establish a causal relationship between the disease and influence of radiation exposure and other harmful factors and conditions during ChNPP accident.</p><p><strong>Results: </strong>The total number of affected adult citizens as on 01.01.2023, compared to 2008, decreased by 511,877 persons, or by 27.90 % (from 1,834,536 to 1,322,659 persons). The number of clean-up workers of the accident decreased from 276,327 (2008) to 162,180 (2023), or by 114,147 people (41.30 %), that means - during the last 15 years, almost every third-second clean-up worker who participated in the liquidation of the consequences of the accident at the ChNPP died. The number of adult victims decreased from 1,558,209 in 2008 to 1,160,479 in 2023, or by 25.52 % (397,730 persons). In the structure of medical expert cases during 2013-2022 the leading place as the reason of disease, disability and death is occupied by oncological diseases - 60.3 %, cerebrovascular diseases and their complications make up 10.00 %, diseases of the cardiovascular system - 19.6 %.</p><p><strong>Conclusion: </strong>In the remote post-accidental period (2013-2023) has increased the need of expertise of the causal relationship of disability and the causes of death with the influence of the ChNPP accident consequences for medical and social support. During 2013-2023 period quick reducing for all categories of affected due to the Chornobyl NPP accident taken place. In the structure of medical expert cases during 2013-2023 the leading place as the reason of disease, disability and death is occupied by oncological, cardiovascular and cerebrovascular diseases.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"424-430"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THYROID CANCER INCIDENCE IN THE POPULATION OF UKRAINE AND ITS EPIDEMIOLOGICAL DETERMINANTS: IONIZING RADIATION AND ENDOCRINE DISRUPTORS.","authors":"A Ye Prysyazhnyuk, D A Bazyka, N A Gudzenko, M M Fuzik, N G Babkina, O M Khukhrianska, S A Danevych","doi":"10.33145/2304-8336-2023-28-176-190","DOIUrl":"10.33145/2304-8336-2023-28-176-190","url":null,"abstract":"<p><strong>Objective: </strong>to estimate the risk of thyroid cancer incidence in the population of Ukraine in connection with its exposure to radioactive iodine fallout of Chornobyl origin and the use of pesticides in agricultural production in the country.</p><p><strong>Object of study: </strong>Incidence rates of thyroid cancer in the population of Ukraine in 2001-2019, average regional radiation doses absorbed by the thyroid because of the Chornobyl accident, the volume of use of various groups of pesticides in the regions of Ukraine.</p><p><strong>Research methods: </strong>statistical, mathematical and cartographic.</p><p><strong>Results: </strong>The study covering the period of 2001-2019, revealed significant temporal and regional differences in the thyroid cancer incidence in the population of the Ukraine regions in 2001-2019. The existence of a significant correlation between the thyroid cancer incidence and the amount of radiation exposure to the thyroid associated with the Chornobyl accident was established. The existence of a significant correlation between the thyroid cancer incidence and the degree of pesticide use intensity in agriculture in the Ukraine regions was established. A significant value of multiple correlation r = 0.5866 (p < 0.05) was found between the thyroid cancer incidence in Ukraine andthe average regional radiation doses and the pesticide use intensity in agricultural production in the country.</p><p><strong>Conclusions: </strong>A reliable value of the multiple correlation between the value of the average regional radiation exposure doses to the thyroid associated with the Chornobyl accident and the degree of pesticide use intensity in the national economy of Ukraine and the thyroid cancer incidence in the population was determined.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"176-190"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"APPLICATIONS OF THREE DIMENSIONAL FACIAL COMPUTED TOMOGRAPHY IN HEAD AND NECK REGION - A REVIEW.","authors":"K Shunmugavelu, H Kannapan, P Vaishnavi, E C Dhinakaran, S Lavanya, B Kumar","doi":"10.33145/2304-8336-2023-28-544-548","DOIUrl":"10.33145/2304-8336-2023-28-544-548","url":null,"abstract":"<p><p>3D facial CT has evolved and revolutionized diagnosis leading to better management. The limitations of conventional 2D methods limit the therapeutic options related to leading to an erroneous treatment. Although 3D facial CT is expensive, its advantages outweigh the cons. They are used in surgery, prosthetic replacement, orthodontics and forensics.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"544-548"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RADIOPHARMACEUTICALS BASED ON FIBROBLAST ACTIVATION PROTEIN INHIBITORS IN ONCOLOGICAL PRACTICE.","authors":"D O Dzhuzha, S D Myasoyedov","doi":"10.33145/2304-8336-2023-28-49-64","DOIUrl":"10.33145/2304-8336-2023-28-49-64","url":null,"abstract":"<p><p>The review is devoted to the application of the new perspective class of radionuclide tracers, based on inhibitors of the fibroblast activation protein (FAP), which is a marker of cancer-associated fibroblasts of microenvironment of most malignant tumors. FAP inhibitors (FAPI) can be labeled by a wide spectrum of radionuclides, which can provide both radionuclide diagnostics and radionuclide therapy. It was showed, that the diagnostic efficacy of FAPI positron emission tomography/computed tomography (PET/CT) in various cancers is similar to 18F-fluorodesoxyglu-cose-PET/CT or superior. The FAPI radiopharmaceuticals (RP) have some advantage compared to 18F-fluorodesoxy-glucose (FDG): no need for special preparation and diet, fast imaging with high contrast, and increased sensitivity in region with high glucose metabolism. FAPI-PET/CT may be an important addition to conventional methods in all stages of diagnostic process in oncology, especially when 18F-FDG cannot be used. It is need to further wide prospective investigations for assessing of diagnostic efficacy of FAPI-RP and determination of indications for its using.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"49-64"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SLEEP QUALITY OF SCHOOLCHILDREN - RESIDENTS OF RADIOACTIVELY CONTAMINATED AREAS DURING THE COVID-19 PANDEMIC.","authors":"V A Poznysh, V Yu Vdovenko, I E Kolpakov, A A Chumak","doi":"10.33145/2304-8336-2023-28-387-403","DOIUrl":"10.33145/2304-8336-2023-28-387-403","url":null,"abstract":"<p><strong>Objective: </strong>to assess the sleep characteristics of middle school children during the COVID-19 pandemic.</p><p><strong>Materials and methods: </strong>Cross-sectional study of sleep characteristics of children - residents of radioactively contaminated areas of Zhytomyr and Rivne regions, who underwent a routine examination in June-July 2020, after their stay from March to June 2020 in conditions of home isolation due to quarantine restrictions during the COVID-19 pandemic. Ninety six students of comprehensive public schools, aged 10-16, took part in the study. Sleep characteristics were assessed using the Pittsburgh Sleep Quality Index (PSQI-U indicator), translated into Ukrainian.</p><p><strong>Results and discussion: </strong>During the period of the introduction of restrictive measures due to the COVID-19 pandemic, certain disturbances of sleep characteristics were observed, the imprint of which was a moderately increased total PSQI-U indicator, which was (3.89 ± 3.20) points. It was established that 19.79 % of children had insufficient total sleep time, and 2.08 % had significant sleep deprivation. The quality of their sleep was described as «rather bad» by 32.29 %, and as «very bad» by 4.17 %; 35.42 % of children complained about sleep disturbances, 67.71 % of children had signs of daytime dysfunction, 18.75 % had symptoms of insomnia. In the logistic regression model, quite significant prognostic factors of insomnia were: female gender (OR = 2.487; 95 % CI: 1.407-4.397); subjective determination of the negative impact of the pandemic on health, regardless of whether the respondent had COVID-19 or not (OR = 1.166; 95 % CI: 1.112-1.222), as well as living in the city (OR = 1.183; 95 % CI: 1.065-315).</p><p><strong>Conclusions: </strong>The introduction of restrictive quarantine measures as a result of the COVID-19 pandemic led to a violation of the daily routine of schoolchildren, which modified the characteristics of sleep. In almost a third of the examined children, the duration and quality of night sleep was insufficient for the physiological restoration of the body's reserves. A late choice of the start of sleep and violations of his hygiene had a critical impact on these indicators.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"387-403"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STATE OF CARDIOVASCULAR SYSTEM IN MILITARIES OF UKRAINE ARMED FORCES UNDER THE WAR WITH RUSSIA.","authors":"D Belyi, O Nastina, G Sydorenko, N Kursina, O Bazyka, O Kovaliov, D Bazyka","doi":"10.33145/2304-8336-2023-28-254-266","DOIUrl":"10.33145/2304-8336-2023-28-254-266","url":null,"abstract":"<p><strong>Objective: </strong>To study the state of cardiovascular system in servicemen (SM) of Ukraine Armed Forces (UAF), who took part in the fight against russian military aggression, and the factors of military service that could cause the occurrence of circulatory system diseases.</p><p><strong>Materials and methods: </strong>The study included 110 men of UAF, who were undergoing examination and treatment at the NRCRM. The average age of the examinees was (46.4 ± 8.8) years. The checkup included an examination by a cardiologist and different diseases experts (as indicated), electrocardiography, Doppler echocardiography, daily monitoring of the electrocardiogram and other necessary studies.</p><p><strong>Results: </strong>Hypertensive heart disease (HHD) was diagnosed in 83.6 % of patients, coronary heart disease (CHD) in 52.8 % ones, including 7.3 % of persons who survived an acute myocardial infarction, heart failure (HF) in 80.9 % of patients. Before the war, 51.8 and 7.3 % of SM suffered from HHD and CHD, respectively. Seven SM sustained a missile wound of extremities and subsequently developed HHD, suggesting a possible association between the two events (Pearson's χ2 = 4.148 with р = 0.042, but р = 0.081 using Fisher's exact test). Out of 18 SM without signs of HHD, 8 had normal body weight and 10 had obesity, when in SM with HHD it was 7 and 15 persons, respectively.Obesity I degree discovered in 10, and II degree in 2 SM. Excess body weight and the degree of obesity had a significant relationship with HHD development (χ2 = 8.995; р = 0.029). The age of patients with CHD (50 persons) was significantly greater than that of patients without CHD (52 persons): (50.1 ± 5.4) vs. (42.0 ± 9.5) years at р = 0.000.</p><p><strong>Conclusions: </strong>Among circulatory system diseases in SM of UAF HHD and CHD were the most common ones. More than half of the SM were sick with HHD before being drafted into the army. During the period of taking part in the war the development of HHD new cases was observed in 35 SM, and CHD in 50 SM. The onset of HHD new cases can be associated with missile wound of extremities and obesity, and new CНD cases with age factor.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"254-266"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STILLBIRTH RATE AND INFANT MORTALITY RATE: STUDY IN THE REGIONS OF UKRAINE MOST INTENSIVELY RADIOCONTAMINATED UPON THE CHORNOBYL NPP ACCIDENT.","authors":"N V Gunko, N V Korotkova, S V Masiuk","doi":"10.33145/2304-8336-2023-28-143-157","DOIUrl":"10.33145/2304-8336-2023-28-143-157","url":null,"abstract":"<p><strong>Objective: </strong>to evaluate the time pattern peculiarities of stillbirth and infant mortality rates in the radiocontaminated territories of Ukraine in the post-accident period on the basis of the national State Statistics Service data.</p><p><strong>Object of the study: </strong>stillbirth and infant mortality rates of population of the most intensively radiocontaminated territories of Ukraine (Lughinskyi, Narodytskyi, Ovrutskyi and Olevskyi districts of Zhytomyrska oblast, Vyshgorodskyi, Ivankivskyi and Poliskyi districts of Kyivska oblast, Rokytnivskyi and Sarnenskyi districts of Rivnenska oblast, and Kozeletskyi and Rypkinskyi districts of Chernihivska oblast).Research materials and methods. Information resources of the State Statistics Service of Ukraine for 1986-2020 regarding the infant mortality and stillbirth rates were used in the study. Demographic, mathematical-statistical, graphical, and software-technological research methods were applies.</p><p><strong>Results and conclusions: </strong>Parameters of infant mortality and stillbirth rates for the 1986-2020 period were studied retrospectively. Significant variations in values were revealed both between the territories and years of survey.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"143-157"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE ROLE OF HEREDITARY PREDISPOSITION (POLYMORPHIC MARKERS OF GLUTATHIONE-S-TRANSFERASE, CATALASE, ENDOTHELIAL NITROGEN OXIDE SYNTHASE GENES) AND SOME ADVERSE ENVIRONMENTAL FACTORS IN THE DEVELOPMENT OF BRONCHO-OBSTRUCTIVE PATHOLOGY IN CHILDREN LIVING IN RADIOACTIVELY CONTAMINATED AREAS.","authors":"I Ye Kolpakov, V Yu Vdovenko, V M Zyhalo, V G Kondrashova, H M Chobotko, L A Raichuk, O S Leonovych","doi":"10.33145/2304-8336-2023-28-329-347","DOIUrl":"10.33145/2304-8336-2023-28-329-347","url":null,"abstract":"<p><strong>Objective: </strong>summarizing the results of many years of research by the authors on the influence of gene polymorphisms encoding xenobiotic biotransformation enzymes (GSTТ1, GSTM1, GSTР1), antioxidant protection (С^262Т of the catalase gene), endothelial nitric oxide synthase (4a/4b VNTR polymorphism of the eNOS gene), and some environmental factors on the occurrence of broncho-obstructive disorders and the development of bronchial asthma in children, residents of radioactively contaminated areas.</p><p><strong>Materials and methods: </strong>The examined school-aged children were residents of radioactively RCA who had no clinical signs of respiratory pathology. Deletion polymorphism of catalase gene (CAT C^262T), polymorphism of glutathione-S-transferase gene (GSTТ1, GSTM1, GSTР1) and the polymorphism in the 4th intron (4a/4b) of the eNOS gene were studied in the molecular genetics laboratory of the State Institution «Reference Center for Molecular Diagnostics of Public Health Ministry of Ukraine». Molecular genetic studies were performed by polymerase chain reaction. The study of the ventilation lung capacity was carried out by the method of computer spirometry based on the data of the «flow-volume» loop analysis. A pharmacological inhalation test with a bronchodilator drug which affects the β2-adrenergic receptors of the lungs was used to detect early changes in the ventilatory lung capacity - bronchial hyperreactivity.</p><p><strong>Results and conclusions: </strong>One of the leading mechanisms, due to which the implementation of hereditary predisposition to bronchial asthma in children living in radioactively contaminated areas is the polymorphism of certain genes of glutathione-S-transferase, catalase, endothelial nitric oxide synthase. With such polymorphic variants of the GST genes, isoforms of enzymes with reduced activity are produced, which limits their ability to effectively neutralize free radicals, which are formed in excess when free radical oxidation processes are activated due to the constant intake of radionuclides with a long half-life into the body of children. Unfavorable factors that increase the risk of developing broncho-obstructive disorders and the likelihood of their implementation in the form of bronchial asthma in children, residents of radioactively contaminated areas, have been identified. It has been established that among them the leading role is played by hereditary predisposition to this disease. On the part of the child, such negative factors were unfavorable conditions of intrauterine development, the presence of signs of exudative-catarrhal diathesis, manifestations of allergies and frequent respiratory diseases from the first months of life.</p>","PeriodicalId":20491,"journal":{"name":"Problemy radiatsiinoi medytsyny ta radiobiolohii","volume":"28 ","pages":"329-347"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139058593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}