EFFECTIVENESS OF THE PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT HUMAN CELLS ІN A RESTING STATE.

Abstract

Objective: to investigate the structural and morphofunctional changes in test system of malignant (cell line A-549) human cells in a resting state exposed to X-rays in the presence of gadolinium-containing photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Photolon» photosensitizer.

Methods: Passaged malignant human cell culture technology, X-ray and red light exposure, cytological and statistical methods.

Results: X-ray exposure at a dose of 10.0 Gy in the presence of photon capture agent «Dotavist» (at a 100 μg/ml nutrient medium concentration) led to death of 75-83 % of malignant cells in a resting state on the 6-8th day of cultivation. Photodynamic exposure (630 nm wavelength red light) in the presence of «Photolon» photosensitizer (200 μg/ml concentration) resulted in death of 69-73 % of malignant cells, respectively. Combination of the photon-capturing technology and photodynamic exposure resulted in death of 90 % of the malignant cells in a phase of steady-state growth on the 8th day of cultivation.

Conclusion: Combination of the photon capture technology (X-ray exposure with gadolinium-containing photoncapture agent «Dotavist» in cytotoxic concentration) and photodynamic exposure in the presence of «Photolon» photosensitizer increased devitalization effectiveness of human non-small cell lung cancer cells (A-549 cell line) being in a steady-state growth phase up to 90 %. Ten percent of cells resistant to the applied technologies retained their proliferative potential, evident as changes in their morphology, genotype and adhesiveness during further cultivation.