Practical Laboratory Medicine最新文献

{"title":"Added value of a connected glucose meter for glycorrhachia assessment","authors":"Laurent Blairon ,&nbsp;Marie Tré-Hardy ,&nbsp;Sophie Collignon ,&nbsp;François Coenen ,&nbsp;Ingrid Beukinga ,&nbsp;Roberto Cupaiolo","doi":"10.1016/j.plabm.2024.e00384","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00384","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer.</p></div><div><h3>Design and Methods</h3><p>Intra-assay and inter-assay accuracies were calculated using residual clinical samples. Accuracies were measured by comparing the results obtained with the glucometer to those from the central laboratory on a large routine chemistry platform.</p></div><div><h3>Results</h3><p>The intra-assay coefficients of variation were between 6.1% and 6.2% for low values (18 mg/dL) and between 5.6% and 6.8% for high values (58 mg/dL). The inter-assay coefficients of variation were between 9.4% and 16.3% for the low values (18 mg/dL) and between 5.7% and 8.7% for the high values (pool; ±75 mg/dL). The regression equation by comparison to the central laboratory was y = 4.08 + 0.82 x, with a coefficient of determination (r²) of 0.95.</p></div><div><h3>Conclusions</h3><p>The measurement of glycorrhachia with a connected glucometer before the analysis in the central laboratory allows a rapid orientation in the deferential diagnosis of a meningitis of viral vs bacterial origin. The response time is fast (6 s) and requires only a small amount of fluid (1.2 μL), which is important in infants, especially since lumbar puncture is an integral part of the investigation of the origin of a fever in this population.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00384"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000301/pdfft?md5=832c69b64e8568ee7d0ec1e53b2808dd&pid=1-s2.0-S2352551724000301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140031400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of plasma collection tubes on N-glycome in human blood samples","authors":"Zejian Zhang ,&nbsp;Xiangyi Cui ,&nbsp;Nan Zhou ,&nbsp;Lisi Zhu ,&nbsp;Yuxiang Zhi ,&nbsp;Shuyang Zhang","doi":"10.1016/j.plabm.2024.e00383","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00383","url":null,"abstract":"<div><h3>Background and aims</h3><p>Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.</p></div><div><h3>Materials and methods</h3><p>We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.</p></div><div><h3>Results</h3><p>Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits.</p></div><div><h3>Conclusion</h3><p>Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00383"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000295/pdfft?md5=2f804ffd65e96a5c5246b7e8c695a672&pid=1-s2.0-S2352551724000295-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of chemiluminescent immunoassay and enzyme-linked immunosorbent assay for detecting phospholipase A2 receptor antibody in primary membranous nephropathy","authors":"Xiaotao Ma ,&nbsp;Ruiting Wang ,&nbsp;Linting Wei ,&nbsp;Pengfei Liu ,&nbsp;Lanmei Jing ,&nbsp;Jinghua Wang ,&nbsp;Wei Dong ,&nbsp;Xuefei Tian ,&nbsp;Rongguo Fu","doi":"10.1016/j.plabm.2024.e00385","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00385","url":null,"abstract":"<div><h3>Objective</h3><p>The accurate detection of phospholipase A2 receptor (PLA2R) autoantibody is crucial in the diagnosis and monitoring of primary membranous nephropathy (pMN). While enzyme-linked immunosorbent assay (ELISA) is the commonly used detection method, its complexity and time-consuming nature pose challenges, especially for small sample sizes. Chemiluminescence immunoassay (CLIA) has emerged as a rapid alternative for clinical immunoassays. This study aims to compare the sensitivity, specificity, and precision of CLIA and ELISA in detecting PLA2R autoantibody.</p></div><div><h3>Method</h3><p>A total of 145 patients with biopsy-confirmed primary membranous nephropathy and 85 patients with non-membranous nephropathy were enrolled in this comparative study. CLIA and ELISA were employed to test all samples for the presence of PLA2R autoantibodies. Statistical analysis of sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) was performed using SPSS 26.0. The diagnostic value of ELISA and CLIA for pMN was analyzed using the ROC curve, and Correlation analysis was performed using Spearman.</p></div><div><h3>Results</h3><p>Serum levels of anti-PLA2R antibody in pMN group were significantly higher than those in nMN group(P &lt; 0.05). The accuracy of CLIA for detecting anti-PLA2R antibody was 76.96%, while ELISA showed an accuracy of 74.78%. The sensitivity for CLIA was 64.83%, compared to 60% for ELISA. However, no statistically significant difference was observed between the two methods (<em>P</em> &gt; 0.05). The overall qualitative agreement of anti-PLA2R detection was 93.35% (95% confidence interval[CI] 89.47–96.3). ROC curve analysis showed that AUC of anti-PLA2R antibody detected by ELISA and CLIA were 0.8737 (95% confidence interval [CI] 0.8270–0.9204), 0.8914 (95% confidence interval [CI]0.8495–0.9332), respectively. The Spearman correlation analysis revealed a significant correlation between them(<em>P</em> &lt; 0.05). Notably, CLIA demonstrated a significant time-saving advantage, particularly when the sample size was less than 200, and especially when it was less than 20.</p></div><div><h3>Conclusion</h3><p>CLIA and ELISA showed similar accuracy and consistency in detecting anti-PLA2R antibody for primary membranous nephropathy. However, CLIA exhibited a significant advantage in terms of automation and time-saving compared to ELISA, particularly for smaller sample sizes. This finding suggests that CLIA has the potential to become a preferred and widely adopted test in the future.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00385"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000313/pdfft?md5=77ed71aee7e179b45c671f2edfe84687&pid=1-s2.0-S2352551724000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between locally versus centrally processed serum procalcitonin during emergency department research evaluation of febrile infants aged 0–60 days","authors":"Cosby G. Arnold ,&nbsp;Prashant Mahajan ,&nbsp;Russell K. Banks ,&nbsp;John M. VanBuren ,&nbsp;Nam K. Tran ,&nbsp;Octavio Ramilo ,&nbsp;Nathan Kuppermann","doi":"10.1016/j.plabm.2024.e00391","DOIUrl":"10.1016/j.plabm.2024.e00391","url":null,"abstract":"<div><h3>Introduction</h3><p>Procalcitonin (PCT) is a useful biomarker in the initial evaluation of febrile infants for serious bacterial infections (SBIs). However, PCT is not always available locally and must at times be frozen and shipped to a reference laboratory for research studies. We sought to compare PCT measured locally versus centrally at a reference laboratory during a research study.</p></div><div><h3>Materials and methods</h3><p>This was a secondary analysis of a multicenter study of febrile infants ≤60 days evaluated for SBIs from June 2016 to April 2019. A PCT cutoff value of 0.5 ng/mL was used to stratify infants at low-versus high-risk of SBIs. Statistical analyses consisted of Spearman's correlation, Bland-Altman difference plotting, Passing-Bablok regression, Deming regression, and Fisher's exact testing at the 0.5 ng/mL threshold.</p></div><div><h3>Results</h3><p>241 febrile infants had PCT levels measured both locally and at the reference laboratory. PCT levels measured locally on 5 different platforms and from the frozen research samples demonstrated strong Spearman's correlation (ρ = 0.83) and had similar mean PCT values with an average relative difference of 0.02%. Eleven infants with SBIs had PCT values &lt; 0.5 ng/mL in both the clinical and research samples. Six other infants had differences in SBI prediction based on PCT values at the 0.5 ng/mL threshold between the clinical and research platforms.</p></div><div><h3>Conclusions</h3><p>We found no significant differences in detection of febrile infants at high risk for SBI based on locally (on multiple platforms) versus centrally processed PCT. Testing at a central reference laboratory after freezing and shipping is an accurate and reliable alternative for research studies or when rapid turnaround is not required.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00391"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000374/pdfft?md5=4f51ab267812173286559ea7c32b5f65&pid=1-s2.0-S2352551724000374-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140275291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are we ready for widespread implementation of lactate POCT in fetal blood sampling? An analytical and clinical verification of the novel statstrip POCT analyzer","authors":"Nienke Spronk ,&nbsp;Madeleine SQ. Kortenhorst ,&nbsp;Jasmijn A. van Balveren","doi":"10.1016/j.plabm.2024.e00375","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00375","url":null,"abstract":"<div><h3>Objectives</h3><p>Currently, pH values are used in fetal scalp blood sampling as a parameter to rule out fetal distress during the delivery. Due to a high number of pre-analytical errors in pH measurements, lactate measurement is already extensively examined as an alternative. Our objectives were to confirm the analytical performance of the StatStrip lactate POCT analyzer and to compare pH and lactate as a marker of fetal distress.</p></div><div><h3>Design</h3><p><em>and methods</em>: Fetal blood scalp, umbilical and arterial blood test results (n = 100) were analyzed to compare the current POC blood gas analyzer including lactate (iSTAT-1) with the new POCT analyzer (StatStrip) to test the analytical performance. Furthermore, in all fetal scalp blood tests with a lactate en pH measurement from 2021 to 2023, clinical delivery data was collected to perform a clinical verification.</p></div><div><h3>Results</h3><p>The lactate concentration on the StatStrip analyzer correlated well with the iSTAT-1 (Pearson's r ≥ 0.95). 73 Fetal scalp blood tests showed 18% discrepant results when comparing pH and lactate with regard to fetal distress and consequent delivery intervention. Lactate showed more false positive results than pH (4 versus 1), but no false negatives as opposed to pH (0 versus 1).</p></div><div><h3>Conclusions</h3><p>The lactate Statstrip and iSTAT-1 POCT analyzers were analytically equivalent. The clinical verification study showed that lactate is a good predictor of fetal distress, although more false positive results were found in our limited dataset. However, unnecessary interventions due to failed pH measurements might be prevented when a lactate measurement is introduced.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00375"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000210/pdfft?md5=264c8d80341769c182b509e0f7e6321b&pid=1-s2.0-S2352551724000210-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140160673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of infliximab, adalimumab, and anti-drug antibodies: Development and validation of new monotest, automated assays on multiparametric instrument","authors":"Helena Cerutti ,&nbsp;Giulia Tesi ,&nbsp;Francesco Petrini ,&nbsp;Tommaso Bandini ,&nbsp;Alessandra Cartocci ,&nbsp;Andrea Ianniello ,&nbsp;Alessia Bogi ,&nbsp;Chiara Muzzi ,&nbsp;Alessandra Brogi","doi":"10.1016/j.plabm.2024.e00374","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00374","url":null,"abstract":"<div><h3>Objective</h3><p>To convert manual ELISA kits to fully automated immunoassays that quantify serum drug levels and anti-drug antibodies levels of infliximab and adalimumab (CHORUS Promonitor kits).</p></div><div><h3>Desing and methods</h3><p>CHORUS Promonitor INFLIXIMAB, CHORUS Promonitor ADALIMUMAB, CHORUS Promonitor ANTI-INFLIXIMAB, and CHORUS Promonitor ANTI-ADALIMUMAB kits were compared with the corresponding Promonitor kits to determine sensitivity and specificity of the assays. For the automated assays, the entire procedure -from samples dilution to final readouts-was performed by CHORUS TRIO instrument (DIESSE, Italy). Residual human serum samples from clinical laboratory investigations and samples resulting from the addition of specific drugs (IFX or ADL) or anti-drug antibodies (anti-IFX or anti-ADL) were used for the characterization and validation of the tests.</p></div><div><h3>Results</h3><p>CHORUS Promonitor kits showed an excellent agreement (Cohen's coefficient = 1) with the Promonitor kits and were linear within predefined ranges. All assays were accurate and repeatable, as an acceptable variability were observed within runs, between runs, lots, and instruments. No difference in detecting the reference drug or biosimilars emerged.</p></div><div><h3>Conclusion</h3><p>During preclinical development, these kits resulted as sensitive, specific, accurate, and able to quantify either the reference drug or the corresponding biosimilars. All these features support their use in clinical practice for therapeutic drug monitoring of patients with inflammatory diseases under treatment with IFX or ADL.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00374"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000209/pdfft?md5=5a0d77dccb5d4f96d63fe3c41f27ee35&pid=1-s2.0-S2352551724000209-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of using real-world free thyroxine data from the US and Europe to enable fast and efficient transfer of reference intervals from one population to another","authors":"Hedwig Kurka ,&nbsp;Peter Dilba ,&nbsp;Carlos Castillo Perez ,&nbsp;Peter Findeisen ,&nbsp;Ignacio Gadea Gironés ,&nbsp;Alex Katayev ,&nbsp;Laura Rodríguez Alonso ,&nbsp;André Valcour ,&nbsp;Thorsten Rehberg ,&nbsp;Benedikt Weber ,&nbsp;Horst Donner ,&nbsp;Anja Thorenz","doi":"10.1016/j.plabm.2024.e00382","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00382","url":null,"abstract":"<div><h3>Objectives</h3><p>The direct approach for determining reference intervals (RIs) is not always practical. This study aimed to generate evidence that a real-world data (RWD) approach could be applied to transfer free thyroxine RIs determined in one population to a second population, presenting an alternative to performing multiple RI determinations.</p></div><div><h3>Design and methods</h3><p>Two datasets (US, n = 10,000; Europe, n = 10,000) were created from existing RWD. Descriptive statistics, density plots and cumulative distributions were produced for each data set and comparisons made. Cumulative probabilities at the lower and upper limits of the RIs were identified using an empirical cumulative distribution function. According to these probabilities, estimated percentiles for each dataset and estimated differences between the two sets of percentiles were obtained by case resampling bootstrapping. The estimated differences were then evaluated against a pre-determined acceptance criterion of ≤7.8% (inter-individual biological variability). The direct approach was used to validate the RWD approach.</p></div><div><h3>Results</h3><p>The RWD approach provided similar descriptive statistics for both populations (mean: US = 16.1 pmol/L, Europe = 16.4 pmol/L; median: US = 15.4 pmol/L, Europe = 15.8 pmol/L). Differences between the estimated percentiles at the upper and lower limits of the RIs fulfilled the pre-determined acceptance criterion and the density plots and cumulative distributions demonstrated population homogeneity. Similar RI distributions were observed using the direct approach.</p></div><div><h3>Conclusions</h3><p>This study provides evidence that a RWD approach can be used to transfer RIs determined in one population to another.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00382"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000283/pdfft?md5=aa43c4abd2673d17687186c2252deb4a&pid=1-s2.0-S2352551724000283-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a whole blood machine learning strategy for distinguishing between bacterial and viral infection in a pediatric hospital setting","authors":"Ridwan B. Ibrahim ,&nbsp;Herda Ona ,&nbsp;Anil K. Chokkalla ,&nbsp;Estella Tam ,&nbsp;Sridevi Devaraj","doi":"10.1016/j.plabm.2024.e00387","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00387","url":null,"abstract":"<div><p>Similar symptoms between viral and bacterial diseases often make diagnosis difficult. This study assessed the clinical performance of the newly cleared whole-blood Bacterial versus Viral Score assay in our pediatric cohort to the previously validated serum assay and emergency department physician diagnosis. This assay shows excellent agreement (R = 0.997) with the serum assay and has great diagnostic accuracy when compared to physician diagnosis.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00387"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000337/pdfft?md5=dba0a944f44ef3bfd57138f27938c1db&pid=1-s2.0-S2352551724000337-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140134836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the efficacy of pooled serum for serum glucose inhouse quality control material in comparison with commercial internal quality control in clinical chemistry laboratory","authors":"Haymanot Tewabe ,&nbsp;Asaye Mitiku ,&nbsp;Abebe Yenesew","doi":"10.1016/j.plabm.2024.e00377","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00377","url":null,"abstract":"<div><h3>Background</h3><p>This study aimed to create an in-house glucose quality control material for humans, addressing the challenge of obtaining high-cost commercially prepared quality control materials in developing countries.</p></div><div><h3>Methods</h3><p>An in-house quality control material for glucose was prepared using a pooled serum sample and analyzed using a fully automated chemistry analyzer following the ISO 80 guidelines. The mean, standard deviation (SD), and coefficient of variance were calculated from the first 30 days of measurement, and the variability was checked over eight months using SPSS software. The study used Pearson's correlation with a 95% confidence interval and a P-value less than 0.05, which was statistically significant.</p></div><div><h3>Results</h3><p>The average mean ± SD of human serum glucose was 185.2 ± 8.4 mg/dL, indicating that the precision between each measurement was better. The prepared in-house quality control material was stable for approximately five months without any significant change in the serum glucose concentration (mg/dl) (p-value&lt;0.05).</p></div><div><h3>Conclusions</h3><p>The study suggests that room-temperature, 2–8 °C, and −20 °C to −30 °C storage of human serum samples for glucose analysis is a viable option, with stable glucose concentrations for up to 30 days. Pooled serum is a cost-effective method for in-house quality control, especially in resource-limited laboratories.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00377"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000234/pdfft?md5=1e926695fe7872c3a3067d9ca3d0ce5c&pid=1-s2.0-S2352551724000234-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140113416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To disinfect or not to disinfect that is the question – Procedure when drawing blood for alcohol measurements in Denmark","authors":"Rebekka Lynge ,&nbsp;Christina I. Kirkvaag ,&nbsp;Ida H. Eilenberger ,&nbsp;Anne M.D. Hansen ,&nbsp;Julie Smith","doi":"10.1016/j.plabm.2024.e00381","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00381","url":null,"abstract":"<div><p>Swabbing with ethanol to disinfect the skin before venipuncture does not bias measurements of blood ethanol, as previously suspected. International evidence-based theory may not always be successfully integrated into local practices, where old customs may remain. So how are the local protocols for swabbing in practice – if they even do swab? Not disinfecting may risk patient safety. We aim to put a focus on the venipuncture disinfection procedure in practice when measuring blood alcohol for clinical matters and if their procedure refers to a guideline.</p><p>Specialized biomedical laboratory scientists (BLS) are typically responsible for the phlebotomy procedure in Denmark, thus questionnaires were sent to the relevant BLS in 2020 to map disinfection procedures in all Danish hospitals and affiliated blood draw clinics (n = 58).</p><p>The response rate was 93% (54/58). We observed an inter-laboratory dissimilarity in swabbing procedures, when measuring blood alcohol: A quarter did not use any disinfectant (26%), while the remaining disinfected with ethanol 55%, isopropanol 13%, and 6% with ethanol/chlorhexidine. Of the five Danish regions, three had a regional guideline (3/5), otherwise the swabbing protocol was locally based. There was a regional difference in disinfecting or not (Chi² p &lt; 0,0001).</p><p>Danish protocols do not always parallel international literature and international guidelines. Not applying disinfectant may jeopardize patient safety. Laboratories are encouraged to work with evidence-based practice or follow newest standardized international guidelines.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":"39 ","pages":"Article e00381"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000271/pdfft?md5=3b28d77d8ceed9608c01af18444d1bc7&pid=1-s2.0-S2352551724000271-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140209426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}