Potato ResearchPub Date : 2024-02-21DOI: 10.1007/s11540-024-09702-7
Carlos Miguel Peraza-Alemán, Ainara López-Maestresalas, Carmen Jarén, Niuton Rubio-Padilla, Silvia Arazuri
{"title":"A Systematized Review on the Applications of Hyperspectral Imaging for Quality Control of Potatoes","authors":"Carlos Miguel Peraza-Alemán, Ainara López-Maestresalas, Carmen Jarén, Niuton Rubio-Padilla, Silvia Arazuri","doi":"10.1007/s11540-024-09702-7","DOIUrl":"https://doi.org/10.1007/s11540-024-09702-7","url":null,"abstract":"<p>The application of hyperspectral imaging (HSI) has gained significant importance in the past decade, particulary in the context of food analysis, including potatoes. However, the current literature lacks a comprehensive systematic review of the application of this technique in potato cultivation. Therefore, the aim of this work was to conduct a systematized review by analysing the most relevant compounds, diseases and stress factors in potatoes using hyperspectral imaging. For this purpose, scientific studies were retrieved through a systematic keyword search in Web of Science and Scopus databases. Studies were only included in the review if they provided at least one set of quantitative data. As a result, a total of 52 unique studies were included in the review. Eligible studies were assigned an in-house developed quality scale identifying them as high, medium or low risk. In most cases the studies were rated as low risk. Finally, a comprehensive overview of the HSI applications in potatoes was performed. It has been observed that most of the selected studies obtained better results using linear methods. In addition, a meta-analysis of studies based on regression and classification was attempted but was not possible as not enough studies were found for a specific variable.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"19 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139927058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato ResearchPub Date : 2024-02-14DOI: 10.1007/s11540-024-09700-9
Michaela Laumer, Adolf Kellermann, Franz-Xaver Maidl, Kurt-Jürgen Hülsbergen, Thomas Ebertseder
{"title":"Development and Validation of an Optoelectronic Method for the Assessment of Frying Colours of French Fries Based on RGB Colour Values","authors":"Michaela Laumer, Adolf Kellermann, Franz-Xaver Maidl, Kurt-Jürgen Hülsbergen, Thomas Ebertseder","doi":"10.1007/s11540-024-09700-9","DOIUrl":"https://doi.org/10.1007/s11540-024-09700-9","url":null,"abstract":"<p>High frying quality in terms of frying colour is an important trait for potatoes meant for French fry production. Determination of frying colour is often performed visually, which might be affected by biases and is therefore less suitable for research. Available laboratory methods also lack the capability to cover the whole sample and distinguish between tuber parts. Additionally, sample destruction is often required for colour analysis. Therefore, a new approach using RGB colour values and the subsequent R/G ratio of French fries to assess frying colour was tested in this research. Over the course of 3 years, 673 samples of the cultivar <i>Innovator</i> were assessed and compared to visual scoring, and some samples were also assessed for glucose content. A high correlation of <i>R</i><sup>2</sup> = 0.8346 between the visual scoring and the optoelectronic assessment was found for 633 samples. The validation data set of 40 samples reached a correlation of <i>R</i><sup>2</sup> = 0.7850 with the implemented model. These results show that French fry frying colour can be described using the R/G ratio. This promising approach is suitable for other researchers as well as industry professionals with further research.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"6 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139751152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato ResearchPub Date : 2024-02-07DOI: 10.1007/s11540-024-09693-5
Ebrahim Salari, Sareh Baghaee-Ravari
{"title":"Utilization of Antagonistic Bacteria for Managing Potato Brown Leaf Spot Caused by Alternaria tenuissima and Plant-Associated Biochemical Changes During Disease Development","authors":"Ebrahim Salari, Sareh Baghaee-Ravari","doi":"10.1007/s11540-024-09693-5","DOIUrl":"https://doi.org/10.1007/s11540-024-09693-5","url":null,"abstract":"<p>The incidence of brown leaf spot, attributed to <i>Alternaria tenuissima</i>, has exhibited an increase within potato fields in Iran. The economic losses caused by brown leaf spot and detection of cross-resistance among commonly used fungicides have incited research towards identifying alternative biocontrol sources to manage this aggressive pathogen in combination with chemicals. Among bacterial isolates that showed inhibition of <i>A. tenuissima</i> growth, the tested biocontrol properties and plant growth-promoting (PGP) characteristics varied. Three strains with antifungal traits and one strain with PGP capacity were chosen for further in vitro and in vivo experiments. They were identified as <i>Bacillus halotolerans</i>-H14, <i>Bacillus</i> sp.-H52, <i>Serratia polymuthica-</i>FR1, and <i>Stenotrophomonas</i> sp.<i>-</i>J29, respectively, using a combination of phenotypic traits and 16S rRNA sequencing. In co-culturing assays with the aforementioned bacterial strains, it was observed that they induced hyphal structural deformation and suppressed spore germination of <i>A. tenuissima.</i> Monitoring of the disease symptoms showed the ability of H14, FR1, and J29 to decrease disease severity under in vivo challenge experiments. Moreover, the role of aforementioned bacteria was studied in activation of potato defence pathways against <i>A. tenuissima</i>. During <i>Solanum tuberosum-A. tenuissima</i> interaction, biochemical attributes related to defence response such as superoxide and hydrogen peroxide production, redox enzymes (catalase, superoxide dismutase, ascorbate peroxidase, and guaiacol peroxidase), phenylalanine ammonia-lyase activity, and accumulation of total phenolics were markedly increased in potato plants pre-treated with <i>Stenotrophomonas</i> sp.-J29 and <i>Serratia plymuthica-</i>FR and post-inoculated with <i>A. tenuissima</i>. This study provides the initial evidence demonstrating the potential role of <i>Bacillus halotolerans</i>, <i>Serratia plymuthica</i>, and <i>Stenotrophomonas</i> sp. as prohibitor of disease progression as well as biological inducer of resistance in potato—<i>A. tenuissima</i> interplay.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"2 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139751161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato ResearchPub Date : 2024-02-07DOI: 10.1007/s11540-024-09690-8
N. K. Pandey, Pynbianglang Kharumnuid, Sant Kumar, S. K. Chakrabarti, Vinay Bhardwaj
{"title":"Economic Impact of Early Bulking and Drought-Tolerant Potato Cultivar Kufri Pukhraj in India","authors":"N. K. Pandey, Pynbianglang Kharumnuid, Sant Kumar, S. K. Chakrabarti, Vinay Bhardwaj","doi":"10.1007/s11540-024-09690-8","DOIUrl":"https://doi.org/10.1007/s11540-024-09690-8","url":null,"abstract":"<p>Adoption of high-yielding, early maturing and drought-resistant varieties is a good adaptation strategy to cope with the adverse effects of climatic events like frequent occurrence of drought and heat stress. Kufri Pukhraj, an early bulking cultivar that is, drought tolerant and moderately resistant to late blight, is the most popular potato variety in India, covering 33% of total potato area. This paper assesses the gross returns from the investment in Kufri Pukhraj using the economic surplus method in a closed economy condition at all-India level. Time-series published data from various sources have been used in this study. A total of 41-year period (1978–2018) is considered to estimate the benefits in an ex-post framework using actual data. The analysis of varietal diversity of potato revealed that 11 out of 66 varieties are most popular and share about 75% of total potato area in India. These varieties are early-to-medium maturing and medium-to-high tolerant to drought. The findings show that R&D investment on Kufri Pukhraj has led to enormous benefits to both consumers and producers. The total surplus accrued to economy is estimated as Rs 540,840 million during 1978 to 2018. The net present value of returns was Rs 157,720 million and Rs 63,600 million at 5% and 8%, respectively. The study has found internal rates of return of 48%, which is a good rate of returns. Sensitivity analyses also showed good returns, with total economic surplus ranging from Rs 293,220 million to Rs 814,950 and internal rates of return ranging between 45 and 50%. Results imply that the investment in R&D of Kufri Pukhraj has proven to be successful and beneficial in India. The study recommends that other popular varieties equipped with other traits should be evaluated for shaping future research policies of potato.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"08 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139751163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato ResearchPub Date : 2024-02-07DOI: 10.1007/s11540-024-09697-1
Manuel Gastelo, Willmer Pérez, Raul Eyzaguirre, Katherine Quispe, Kimberlayn Sanabria, Carolina Bastos, Ronal Otiniano, Juan M. Pérez, Alejandro Mendoza, Trinidad Unda, Jorge Andrade
{"title":"New Potato Varieties Resistant to Late Blight and with High Quality for French Fries Generated in Peru","authors":"Manuel Gastelo, Willmer Pérez, Raul Eyzaguirre, Katherine Quispe, Kimberlayn Sanabria, Carolina Bastos, Ronal Otiniano, Juan M. Pérez, Alejandro Mendoza, Trinidad Unda, Jorge Andrade","doi":"10.1007/s11540-024-09697-1","DOIUrl":"https://doi.org/10.1007/s11540-024-09697-1","url":null,"abstract":"<p>Potato is one of the main crops worldwide and generates income for small-scale farmers, and it is a primary component of the diet of rural inhabitants, especially in developing countries. In Peru, the demand for French fries in traditional restaurants, rotisserie chicken (“Pollerias”), and fast-food restaurants is increasing every day. However, supply is covered by imported pre-fried potatoes. During the 2019–2020 and 2020–2021 growing seasons, ten potato clones belong to the International Potato Center (CIP) breeding program, and two Peruvian varieties used for frying as controls UNICA (CIP392797.22) and INIA 303-CANCHAN (CIP380389.1) were tested in 13 trials in farmers' fields placed in the north, center, and south of Peru using a randomized complete block design with three replications of 150 plants. Late blight severity and yield were evaluated under field conditions, and the contents of dry matter and reducing sugars were determined with laboratory tests. Clones CIP395123.6, CIP396026.101, and CIP396034.103 were selected for their high level of resistance to late blight, yields over 30 t/ha, with more than 20% dry matter, less than 0.20% reducing sugars and excellent quality for French fries’ sticks. Selected clones have been registered in the official register of cultivars of Peru, as new varieties. The standard of living of small and medium-scale potato farmers can be improved with the production of these new varieties, which support the health of producers and consumers and the environment. New potato varieties can be used as parents in breeding programs around the world.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"21 2 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139751540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quality Evaluation and Fourier Transform Infrared (FTIR) Spectroscopy Characterization of Vodka Produced from Malted and Un-malted Potatoes Using Saccharomyces cerevisiae (MK680910)","authors":"Vishal Luthra, Keshani Bhushan, Gurvinder Singh Kocher, Arashdeep Singh","doi":"10.1007/s11540-024-09694-4","DOIUrl":"https://doi.org/10.1007/s11540-024-09694-4","url":null,"abstract":"<p>The present study aims to evaluate the suitability of un-malted (raw version) and malted (sprouted) potato variety Kufri Pukhraj for the production of vodka using the brewing yeast strain <i>Saccharomyces cerevisiae</i> (MK680910). Both un-malted and malted potatoes were subjected to 72 h of fermentation and valuated for changes in total sugars, reducing sugars and ethanol conversion. Results showed that with an increase in fermentation time from 24 to 72 h, the content of total and reducing sugars decreased significantly (<i>p</i> < 0.05), resulting in a significant increase in the ethanol concentration. After 72 h of fermentation, malted potato produced the best results, with a greater substrate-to-product conversion (0.47 g ethanol g<sup>−1</sup> reducing sugars), a higher fermentation yield (91.97%), and higher ethanol content (9.82 percent v/v). Following fermentation, double distillation, clarification with activated charcoal, blending, and sensory analysis with different flavours were conducted. Vodka with ethanol concentration of 41.3% v/v was produced having 82.6 proof. Vodka blended with lemon was found to be more preferred with mean score of 8 and was of standard quality. The structural characterisation of the FTIR spectrum band at 1000 cm<sup>−1</sup> analysis correlates to C-O stretching in the volatile components and showed the presence of ethanol in the vodka.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"16 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139751423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato ResearchPub Date : 2024-02-02DOI: 10.1007/s11540-024-09699-z
T. Archana, A. Kamalakannan, C. Gopalakrishnan, I. Johnson, L. Rajendran, S. Varanavasiappan, S. Rajesh, S. P. Thamaraiselvi
{"title":"Detection of Indian Isolates of Soil and Tuber Borne Ralstonia solanacearum (Smith) Infecting Potato (Solanum tuberosum L.) Through a Colorimetric LAMP Assay","authors":"T. Archana, A. Kamalakannan, C. Gopalakrishnan, I. Johnson, L. Rajendran, S. Varanavasiappan, S. Rajesh, S. P. Thamaraiselvi","doi":"10.1007/s11540-024-09699-z","DOIUrl":"https://doi.org/10.1007/s11540-024-09699-z","url":null,"abstract":"<p>Bacterial wilt caused by <i>Ralstonia solanacearum</i>, is one of the most notorious plant diseases of potato and causes severe yield losses globally. <i>R. solanacearum</i> produces fluidal white coloured colonies with a light pink centre on casamino acid-peptone-glucose medium. Bacterial wilt pathogen is soil as well as tuber borne, so early detection of bacterial wilt pathogen is necessary to take up timely management practices. Traditional methods used for detection of <i>R. solanacearum</i> are labour-intensive, expensive, time-consuming and can be carried out only in well-equipped laboratories. Hence, the present study was aimed to develop a loop-mediated isothermal amplification (LAMP) assay for <i>R. solanacearum</i>, as an alternative approach for quick and efficient detection of the bacterial wilt. LAMP reaction mix was optimized by adjusting the concentrations of MgSO<sub>4,</sub> dNTPs, betaine, and time, temperature etc. The optimum temperature and time for the detection of <i>R. solanacearum</i> were 65 °C and 50 min, respectively. The positive samples showed colour change from violet and were reconfirmed in 2% agarose gel electrophoresis which produced ladder like bands. The LAMP assay developed was highly specific to detect <i>R. solanacearum</i> from other bacteria and sensitive with a lowest detection limit of 10 pg/µl of template DNA. The developed LAMP assay was validated with <i>R. solanacearum</i> isolates, infected stems, tubers and soil, and also, it was capable of detecting latent infection of <i>R. solanacearum</i> in seed tubers. Hence, the LAMP assay protocol provides a rapid, specific and sensitive tool for the latent detection of <i>R. solanacearum</i> in seed potato tubers.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"6 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Understanding the Significance of Weather and Soil Microclimate for Improvement of Potato Yield Using SUBSTOR and Statistical Models","authors":"Poonam Biswal, Dillip Kumar Swain, Madan Kumar Jha","doi":"10.1007/s11540-024-09698-0","DOIUrl":"https://doi.org/10.1007/s11540-024-09698-0","url":null,"abstract":"<p>Potato (<i>Solanum tuberosum</i>) production at subtropical latitude is highly influenced by varying weather and soil microclimate. There is a need to quantify the requirement of key weather and soil climatic variables during the critical growth stages of potato. The present investigation was carried out to study the effect of varying weather and soil microclimatic factors during critical growth stages on potato yield in the subtropical climate. Field experiments were conducted in 2018–2019 and 2019–2020 at Kharagpur, India, to determine the relationship between key weather as well as soil microclimate factors and potato tuber yield. The experimental data were used for validation of the crop model DSSAT-SUBSTOR-Potato and simulation of potato tuber yield for varying weather. The effects of three weather variables, i.e. maximum temperature (<i>T</i><sub>max</sub>), minimum temperature (<i>T</i><sub>min</sub>), and solar radiation (<i>S</i><sub>rad</sub>), and two soil variables, i.e. soil moisture (SM) content and soil temperature (ST), on potato yield were assessed through regression analysis. During the ‘Emergence to tuber initiation’ stage, the factors <i>T</i><sub>max</sub>, <i>S</i><sub>rad</sub>, and SM and during ‘Tuber initiation to maturity’ stage, the factors <i>S</i><sub>rad</sub> and SM had significant effects (<i>p</i> ≤ 0.05) on potato production. The optimum values of <i>T</i><sub>max</sub> were 17.58 (± 1.97) and 27.39 (± 0.9) °C; <i>S</i><sub>rad</sub> values were 14.28 (± 2.03) and 19.32 (± 1.96) MJ m<sup>−2</sup> day<sup>−1</sup>; and SM values were 21.79 (± 2.35) and 21.77 (± 1.74) % during ‘emergence to tuber initiation’ and ‘tuber initiation to maturity’ stages, respectively, for increased potato production. The results stated the need for an optimum planting window for providing favourable weather and soil microclimate for potato production in subtropical climate.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"17 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139649053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Potato ResearchPub Date : 2024-01-27DOI: 10.1007/s11540-024-09692-6
M. Harun-Or-Rashid, S. M. Shahinul Islam, M. A. Bari Miah, Sreeramanan Subramaniam
{"title":"Evaluation of the Thermo-tolerance Effect on Cell Suspension Culture in Potato (Solanum tuberosum L.)","authors":"M. Harun-Or-Rashid, S. M. Shahinul Islam, M. A. Bari Miah, Sreeramanan Subramaniam","doi":"10.1007/s11540-024-09692-6","DOIUrl":"https://doi.org/10.1007/s11540-024-09692-6","url":null,"abstract":"<p>In this study, the effect of heat stress pretreatment factors on cell suspension culture of potato was investigated. The duration of stress pretreatment at 5–50 °C for 20 min was evaluated by measuring cell growth and viability using the trypan blue exclusion method. Data were collected by measuring the dry weight of suspended cells, duration of heat stress, growth, and biomass content at the respective time points. Cell viability was negatively affected by 85 to 25% after pretreatment with heat stress between 35 and 50 °C. However, suspended cells exposed to 30 °C and 35 °C during the recovery period began to regain viability at 75% and 69%, respectively, after 55 h. In suspended cells treated at 40 °C or high temperatures, a complete decrease in cell viability was observed, resulting in no recovery of growth. In potato cell suspension cultures, heat treatments at 40 °C or above for 20 min not only have an immediate effect on cell viability, but also lead to subsequent cell death. Effective viability tests showed the natural heat tolerance of potato plants in cell suspension cultures. The basic parameters of cellular response to heat determined in this study will be helpful in selecting heat-tolerant potato varieties by establishing in vitro cell suspension cultures.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"62 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139581618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhancing Cadmium Stress Tolerance in Potato Plants Through Overexpression of the VvWRKY2 Transcription Factor","authors":"Nour Chiab, Safa Charfeddine, Mariam Ayadi, Yosr Abdelkafi, Rim Mzid, Radhia Gargouri-Bouzid, Oumèma Nouri-Ellouz","doi":"10.1007/s11540-023-09687-9","DOIUrl":"https://doi.org/10.1007/s11540-023-09687-9","url":null,"abstract":"<p><i>WRKY</i> transcription factors (TF) are identified as important regulating plant proteins involved in stress response signaling pathways. Overexpression of these transcription factors in plants improved plant biotic and abiotic stress responses. In this context, we have envisaged transferring a cDNA encoding the grapevine <i>VvWRKY2</i>TF in potato plants. Four transgenic lines were selected (BFW2A, BFW2C, BFW2D, and BFW2F). In the present study, their response to Cadmium (Cd) stress (50, 100, 150, and 300 μM) was evaluated in vitro. Cadmium is recognized as being among the most harmful heavy metals to plants. Its accumulation in plant cells and tissues disturbs cell homeostasis and causes numerous metabolic damages that affect productivity. The wildtype (WT) plants from the BF15 potato variety and the transgenic plants overexpressing <i>VvWRKY2</i>TF were submitted to cadmium in vitro stress for 20 days. Plant growth and oxidative stress parameters were followed in these plants. All transgenic plants appeared more vigorous than WT. The BFW2A, BFW2C, and BFW2D lines showed better stem development rates than the WT and BFW2F lines. Malondialdehyde (MDA) production in both roots and leaves was reduced in BFW2A, BFW2C, and BFW2D plants as compared to BFW2F and WT plants. This result was associated with the best antioxidant activities of superoxide dismutase (SOD) and catalase (CAT) displayed by these genetically modified lines suggesting their better adaptation to Cd stress conditions. Cd accumulation in plant tissues was investigated, and higher levels of Cd were found in transgenic plants than in WT plants. These findings point to a functional Cd sequestration mechanism in the roots of transgenic plants expressing <i>VvWRKY2</i>. These findings imply that the <i>VvWRKY2</i> TF is implicated in heavy metal response signaling processes. Its overexpression in plants may be an efficient strategy to reduce the negative effects of Cd stress, promoting the growth patterns and the activity of reactive oxygen species-scavenging enzymes in potato plants.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\u0000","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":"98 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139509686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}