Polish journal of veterinary sciences最新文献_第3页

Effect of shoeing horses with eggbar shoes and shoes with wedge pads on blood flow parameters in the lateral digital artery in the Doppler ultrasound. 用蛋棒鞋和楔形垫鞋给马穿鞋对多普勒超声指外侧动脉血流参数的影响。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145021

M Mieszkowska, P Holak, Y Zhalniarovich, M Mieszkowski, K Domagalska-Stomska

{"title":"Effect of shoeing horses with eggbar shoes and shoes with wedge pads on blood flow parameters in the lateral digital artery in the Doppler ultrasound.","authors":"M Mieszkowska, P Holak, Y Zhalniarovich, M Mieszkowski, K Domagalska-Stomska","doi":"10.24425/pjvs.2023.145021","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145021","url":null,"abstract":"A sound knowledge of horseshoe impact on blood flow parameters is required for making shoeing decisions and selecting the most appropriate types of shoes. The aim of this study was to determine the effect of horse shoeing with egg bar shoes and shoes with wedge pads on blood flow parameters in the lateral palmar digital artery measured by Doppler ultrasound. The study was conducted on 16 horses divided into two groups. Horses from group 1 were shod with egg bar shoes. Horses from group 2 were shod with shoes with wedge pads. Doppler ultrasound parameters of the lateral palmar digital artery at the level of the metacarpophalangeal joint were evaluated. Doppler tests were performed before and after shoeing within a monthly interval. The results of the study indicate that egg bar shoes have a greater impact on blood circulation in the distal part of the equine limb than shoes with wedge pads. However, the only parameters to have changed substantially after shoeing with egg bar shoes were end-diastolic velocity (EDV) and mean velocity (Vmn) in the lateral palmar digital artery. A low-resistance blood flow pattern was noted before shoeing. After shoeing in group 1, it remained unchanged in 5 horses, whereas a high-resistance pattern was observed in 3 animals. A low-resistance blood flow pattern was noted in all group 2 horses after shoeing. The difference between the analyzed shoeing techniques could be attributed to increased pressure in the heel bulb area in horses shod with egg bar shoes. Wedge pads shift the load away from the heel bulbs, which might reduce the pressure on the palmar digital vessels and exert a smaller influence on the parameters measured in the Doppler ultrasound test.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10109664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR. 采用钯化合物预处理后实时荧光定量PCR方法对包膜DNA病毒进行感染性鉴定。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145024

M Krzyzankova, M Krasna, J Prodelalova, P Vasickova

{"title":"A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR.","authors":"M Krzyzankova, M Krasna, J Prodelalova, P Vasickova","doi":"10.24425/pjvs.2023.145024","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145024","url":null,"abstract":"Cultivation-based assays represent the gold standard for the assessment of virus infectivity; however, they are time-consuming and not suitable for every virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. This study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, paying attention to two significant pathogens of livestock - bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl 2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl 2-COD) produced the highest differences found between native and heat- -treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 min, 4°C) were applied on both virus genera and the heat inactivation profiles were assessed. A significant decrease in the detected quantity of BoHV-1 DNA and ASFV DNA after heat-treatment (60°C and 95°C) and consequent incubation with Pd compounds was observed. BB-PdCl 2 and PdCl 2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses such as BoHV-1 or ASFV.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MGF360-12L of ASFV-SY18 is an immune-evasion protein that inhibits host type I IFN, NF-κB, and JAK/STAT pathways. ASFV-SY18的MGF360-12L是一种免疫逃避蛋白，可抑制宿主I型IFN、NF-κB和JAK/STAT通路。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145013

Q Chen, X X Wang, S W Jiang, X T Gao, S Y Huang, Y Liang, H Jia, H F Zhu

{"title":"MGF360-12L of ASFV-SY18 is an immune-evasion protein that inhibits host type I IFN, NF-κB, and JAK/STAT pathways.","authors":"Q Chen, X X Wang, S W Jiang, X T Gao, S Y Huang, Y Liang, H Jia, H F Zhu","doi":"10.24425/pjvs.2023.145013","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145013","url":null,"abstract":"African swine fever virus (ASFV) causes feverous and hemorrhagic disease of domestic pigs and European wild boars with high mortality, yet no commercial vaccine is currently available. Several ASFV strains with natural deletion or gene-targeted knockout of multiple MGF360 and MGF505 genes are attenuated in vitro and in vivo, and can offer full protection against homologous challenge. However, the mechanisms underlying the protection are not fully understood. This study aims to investigate the effects of MGF360-12L of ASFV-SY18 on the cGAS-STING signaling pathway and explore the potential mechanisms. We identified that ASFV-SY18 MGF360-12L could inhibit cGAS-STING, TBK1, or IRF3-5D-stimulated IFN-β expression and ISRE activation. Specifically, MGF360-12L inhibits both the activation of PRD(III-I) in a dose-dependent manner, and suppresses the exogenous expression of TBK1 and IRF3-5D. MGF360-12L could block NF-κB activation induced by overexpression of cGAS-STING, TBK1, IKKβ. Downstream of the IFN-β signaling, MGF360-12L blocks the ISRE promoter activation by reducing total protein level of IRF9. Moreover, MGF360-12L protein can inhibit IFN-β-mediated antiviral effects. In conclusion, our findings suggest that MGF360-12L is a multifunctional immune-evasion protein that inhibits both the expression and effect of IFN-β, which could partially explain the attenuation of relevant gene-deleted ASFV strains, and shed light on the development of efficient ASFV live attenuated vaccines in the future.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9192659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of tilmicosin and diclofenac sodium combination on cardiac biomarkers in sheep. 替尔米考星与双氯芬酸钠联合用药对绵羊心脏生物标志物的影响。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145001

R Yildiz, D Durna Corum, O Corum, M Ider, O Atik, M Ok, K Uney

{"title":"The effect of tilmicosin and diclofenac sodium combination on cardiac biomarkers in sheep.","authors":"R Yildiz, D Durna Corum, O Corum, M Ider, O Atik, M Ok, K Uney","doi":"10.24425/pjvs.2023.145001","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145001","url":null,"abstract":"The aim of this study was to investigate the cardiotoxic effect of the combination of tilmicosin and diclofenac sodium in sheep. Thirty-two sheep were used and were randomly divided into four equal groups as tilmicosin (T), diclofenac sodium (D), tilmicosin+diclofenac sodium (TD) and control (C) group. Group T received a single dose of tilmicosin, Group D was administered diclofenac sodium once a day for 3 days, and group TD was administered diclofenac and tilmicosin at the same doses as group T and D. Group C received NaCl in a similar way. The blood samples were taken before dosing and at 4th, 8th, 24th and 72nd hour post-dosing for measurement of cardiac markers such as H-FABP, cTn-I, CK-MB. H-FABP level of group TD was found to be significantly (p⟨0.05) higher than of group C at the 8th, 24th and 72nd hour and group D and T at the 72nd hour. cTn-I and CK-MB levels of group TD were found significantly (p⟨0.05) higher compared with other groups. In conclusion, the combined use of tilmicosin and diclofenac in sheep causes an increase in cardiac biomarkers and it can be stated that this combination of drugs may cause cardiac damage.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9192655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of emerging ruminant viruses in goats in Poland. 波兰山羊中新出现的反刍病毒的发生。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145015

M Larska, W Socha, J Rola

引用次数: 0

Investigation of Salmonella spp. and Escherichia coli in the Snake-eyed lizard (Ophisops elegans) (Sauria, Lacertidae) in the Çankırı Province of Turkey. 对土耳其Çankırı省蛇眼蜥蜴（秀丽隐尾蛇）（蜥科，蜥蜴科）中沙门氏菌和大肠杆菌的调查。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145009

S Tarhane, E Bozkurt, F Büyük

{"title":"Investigation of Salmonella spp. and Escherichia coli in the Snake-eyed lizard (Ophisops elegans) (Sauria, Lacertidae) in the Çankırı Province of Turkey.","authors":"S Tarhane, E Bozkurt, F Büyük","doi":"10.24425/pjvs.2023.145009","DOIUrl":"10.24425/pjvs.2023.145009","url":null,"abstract":"Zoonoses are frequently associated with wild animals. Research on reptiles either living in their natural habitat or kept as pet animals has shown that these animals frequently serve as the asymptomatic hosts of bacterial zoonotic agents, including Salmonella spp. and Escherichia coli. Studies have shown the potential of reptiles to transmit these pathogens to humans and other animals. Epidemiological research on the herpetofauna of various regions has demonstrated the high potential of reptiles as a reservoir of Salmonella spp. In the present study, Salmonella spp. were not isolated or identified from the snake-eyed lizard. Out of 150 cloacal swab samples of snake-eyed lizard 25 (16.7%) E. coli were isolated and out of these 4 (2.7%) were identified to be E. coli O157:H7 by PCR. The results suggest that Ophisops elegans could be involved in the transmission of E. coli, rather than Salmonella spp. This study demonstrates for the first time that the snake-eyed lizard acts as a cloacal carrier of E. coli O157:H7 and presents data that may aid in preventing the transmission of this strain to humans.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9192658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus. 建立基于E1基因taqman实时PCR定量检测Getah病毒的方法。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145003

A Lin, X Hu, S Cui, T Yang, Z Zhang, P Li, M Guo, Y Lu

{"title":"Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus.","authors":"A Lin, X Hu, S Cui, T Yang, Z Zhang, P Li, M Guo, Y Lu","doi":"10.24425/pjvs.2023.145003","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145003","url":null,"abstract":"To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9192660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of antimicrobial resistance genes in intestinal content from Coyote (Canis latrans). 土狼(Canis latrans)肠道内容物耐药基因的鉴定。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145016

J J López-Islas, E T Méndez-Olvera, T Reyes C, D Martínez-Gómez

引用次数: 1

A review of tuberculosis and parasitic disease co-infection in ungulates, with regard to the potential threat to European bison (Bison bonasus). 关于对欧洲野牛潜在威胁的有蹄类动物结核病和寄生虫病合并感染的综述。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145018

M Gałązka, A Didkowska, K Anusz, A Pyziel-Serafin

引用次数: 0

Clinical effects of combined Lactobacillus paracasei and kestose on canine atopic dermatitis. 副干酪乳杆菌与酮症糖联合治疗犬特应性皮炎的临床疗效。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145014

K Kawano, K Iyori, N Kondo, S Yamakawa, T Fujii, K Funasaka, Y Hirooka, T Tochio

{"title":"Clinical effects of combined Lactobacillus paracasei and kestose on canine atopic dermatitis.","authors":"K Kawano, K Iyori, N Kondo, S Yamakawa, T Fujii, K Funasaka, Y Hirooka, T Tochio","doi":"10.24425/pjvs.2023.145014","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145014","url":null,"abstract":"Probiotics and prebiotics are viable bacteria with beneficial effects on the host and components that selectively act on the beneficial commensal bacteria, respectively. The combined use of probiotics and prebiotics is termed synbiotics. Probiotic intake improves dysbiosis in the intestinal microbiota and can positively affect canine atopic dermatitis (CAD). However, clinical studies on improvements in CAD using synbiotics remain limited. In this study, 15 dogs with CAD who received prednisolone, a synthetic glucocorticoid (GC) used in the treatment of CAD, for more than 90 days were continuously treated with Lactobacillus paracasei M-1 from fermented food as a probiotic, and trisaccharide kestose as a prebiotic, for 90 days to determine their synbiotic effects on CAD. The CAD symptoms were evaluated using the canine atopic dermatitis lesion index (CADLI) and pruritus visual analog scores (PVAS) at 30, 60 and 90 days after synbiotic administration. The total prednisolone use for 90 days pre- and post-administration was also evaluated. Synbiotic administration significantly reduced the CADLI (pre: median, 28.0 [22.0-32.0]; 30 days: median, 20.0 [20.0-28.0]; 60 days: median, 20.0 [10.0-21.0]; 90 days: median, 12.0 [10.0-19.0]) and PVAS (pre: median, 6.0 [5.0-7.0]; 30 days: median, 3.0 [3.0-3.5]; 60 days: median, 3.0 [3.0-3.5]; 90 days: median, 2.0 [2.0-3.5]) scores, and reduced the total prednisone use over 90 days (pre: 112.0 [25-450] mg; post: 80.0 [18.-300.0] mg; p⟨0.001) in the 15 dogs. Thus, the synbiotic activity of L. paracasei M-1 and trisaccharide kestose can improve CAD.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1