Polish journal of veterinary sciences最新文献_第2页

Concomitant virus-induced gastrointestinal infection in dogs. 犬并发病毒引起的胃肠道感染。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145023

H S Saltık

{"title":"Concomitant virus-induced gastrointestinal infection in dogs.","authors":"H S Saltık","doi":"10.24425/pjvs.2023.145023","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145023","url":null,"abstract":"Many viruses are involved in concomitant infections, which are prevalent in nature. In mixed infections, one or both infectious agents may be increased, reduced, or both may be increased while the other is suppressed. Canine distemper virus (CDV) and Canine parvovirus- 2 (CPV-2) are important causes of gastroenteritis in dogs. Detection of these viruses is challenging since the symptoms are very similar. CDV is a member of the morbillivirus genus in the Paramyxoviridae family, and CPV-2 is a member of the Protoparvovirus genus in the Parvoviridae family; and both predominantly affect puppies and induce gastrointestinal symptoms in dogs. The purpose of this study was to contribute to the differential diagnosis of dogs with gastrointestinal symptoms. A PCR technique with specific primers was used to identify CDV and CPV-2 infections in gastroenteric dogs, and clinical changes in the infected dogs were monitored. The VP2 structural gene of CPV and the nucleocapsid gene of CDV were partially amplified in the study. PCR amplified the partial fragments of the CDV nucleocapsid (287 bp) and CPV-2 VP2 proteins (583 bp) from feces. In total, 3 out of 36 stool samples were positive for CDV and CPV-2 in the same dogs. Gasterointestinal symptoms also supported the diagnosis of concomitant infection with CDV and CPV-2 in these dogs. Dehydration and diarrhea in dogs can be signs of various diseases, such as viral, bacterial, and parasitic infections. After the elimination of non-viral pathogens, CDV and CPV-2 should also be simultaneously investigated to establish what is causing these symptoms. This study demonstrates the potential utility of correct diagnosis for the control of viral infection in dogs, but more research with a broader use of PCR-based detections is needed to assess its impact on differential diagnosis for concomitant infections.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eradication of carriers of Complex Vertebral Malformation (CVM) and Brachyspina in Polish Holstein-Friesian bulls. 波兰荷尔斯泰因-弗里西亚公牛复杂椎体畸形(CVM)和短棘携带者的根除。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145031

S Kamiński

引用次数: 0

Comparison of the effect of zinc oxide nanoparticles and extract of Acorus calamus applied topically on surgical wounds inflicted on the skin of rabbits. 氧化锌纳米颗粒与菖蒲提取物局部应用于兔皮肤外科创面的效果比较。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145035

A Abbas, S A Muhammad, A Ashar, S A Mehfooz, A Rauf, M Bakhsh, T Nadeem, H Fu

{"title":"Comparison of the effect of zinc oxide nanoparticles and extract of Acorus calamus applied topically on surgical wounds inflicted on the skin of rabbits.","authors":"A Abbas, S A Muhammad, A Ashar, S A Mehfooz, A Rauf, M Bakhsh, T Nadeem, H Fu","doi":"10.24425/pjvs.2023.145035","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145035","url":null,"abstract":"Antibiotics are used for postsurgical wound healing purposes but unfortunately, resistance against them demands some alternatives for quick recovery. Sepsis of wounds is a challenge for medical as well as veterinary professionals. Nanoparticles have significant advantages in wound treatment and drug resistance reversal. This study was conducted to appreciate emerging alternates of antibiotics like zinc oxide nanoparticles and plant extracts in topical application. Zinc oxide is considered a good wound healer and its nanoparticles are easy to access. So, the efficacies of zinc oxide nanoparticles and sweet flag plant extract ointments were tested to compare modern and traditional therapeutics as sweet flag is considered a pure medicinal plant. Rabbits were selected for this study due to the healing properties of their skin. Wounds were inflicted on the thoracolumbar region and treated for 29 days post-surgically daily with normal saline and the ointment of zinc oxide nanoparticles and sweet flag extract ointment, prepared in a hydrophilic solvent. Wound shrinkage was observed daily and histopathological analysis was made and results were compared. Zinc oxide nanoparticles ointment showed the most satisfactory results for every parameter included in the study. No side effects of its topical application were observed. Healing was normal without any complications. The preparations of zinc oxide nanoparticles may help in the era of antibiotic resistance as topical drugs in the future.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Blastocystis subtype ST25 in calves in Turkey. 土耳其犊牛中首次报道ST25亚型囊虫。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145022

B Aslan Çelik

引用次数: 0

Protective effectiveness of anise against testicular ischemia and reperfusion injury: An experimental study in rats. 茴香对睾丸缺血和再灌注损伤的保护作用：大鼠实验研究

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145029

E Yildizhan, M Akkus, B V Ulger, F Asır, S Söker, E Gündüz, M Rençber, M Barçin

{"title":"Protective effectiveness of anise against testicular ischemia and reperfusion injury: An experimental study in rats.","authors":"E Yildizhan, M Akkus, B V Ulger, F Asır, S Söker, E Gündüz, M Rençber, M Barçin","doi":"10.24425/pjvs.2023.145029","DOIUrl":"10.24425/pjvs.2023.145029","url":null,"abstract":"Testicular torsion is a frequently encountered clinical condition that requires urgent treatment. The aim of this study is to investigate the efficacy of Anise (Pimpinella anisum L.) in treating the pathological condition due to ischemia and reperfusion injury by using biochemical, histopathological and immunohistochemical methods. A total of 6 groups were formed with 8 male Wistar Albino rats in each group. Group 1 (n=8): control group, Group 2 (n=8): Anise aqueous solution was given orally 5 ml/kg by gavage for 30 days. Group 3 (n=8): Ischemia and Reperfusion (I/R) group, bilateral testicles were rotated 270° and reperfused after 30 minutes of ischemia. Group 4 (n=8): I/R+ Anise group, Group 5 (n=8): Anise+ I/R group and Group 6 (n=8): Anise+ I/R+ Anise group. The results of the Anise group and the Control group were similar. However, the damage in the I/R group was considerably more severe than in any of the other study groups. While it was observed that spermatogenic cells started to regenerate in the I/R+Anise group, edema and congestion were observed in the Anise+I/R group. In the Anise+I/R+Anise group, all histological findings and biochemical parameters were similar to those of the control group. It was observed that anise had protective effects in ischemia and reperfusion injury in rat testicles.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection, typing, and virulence potential of Salmonella Serotypes isolated from poultry feeds. 从家禽饲料中分离的沙门氏菌血清型的分子检测、分型和毒力潜力。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145028

G Shahbazi, J Shayegh, C Ghazaei, M H M Ghazani, S Hanifian

{"title":"Molecular detection, typing, and virulence potential of Salmonella Serotypes isolated from poultry feeds.","authors":"G Shahbazi, J Shayegh, C Ghazaei, M H M Ghazani, S Hanifian","doi":"10.24425/pjvs.2023.145028","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145028","url":null,"abstract":"Salmonella contamination in poultry feed is one of the main issues in poultry industry and public health. The aim of the present study was molecular detection and typing of Salmonella serotypes isolated from poultry feeds. Moreover, we determined the antibiotic resistance pattern and the ability of biofilm formation in the serotypes. To this end, eighty feed samples were collected from aviculture depots. Salmonella serotypes were identified by culture and PCR methods. For serological identification, a slide agglutination test was used. BOXAIR and rep-PCR methods were applied to evaluate the diversity of serotypes. The disc diffusion method was performed to evaluate the antibiotic susceptibility of serotypes to sixteen antibiotics. Biofilm formation was also assessed by the microtiter-plate test. From a total of 80 feed samples, 30 samples were contaminated with Salmonella spp., which were divided into 5 different serotypes belonging to B, C, and D serogroups. BOXAIR-PCR (D value [DI] 0.985) and rep-PCR (DI 0.991) fingerprinting of isolates revealed 23 and 19 reproducible fingerprint patterns, respectively. A higher antibiotic resistance was observed to ampicillin and doxycycline (100% each), followed by chloramphenicol (83.33%) and tetracycline (73.33%). Multidrug resistance (MDR) was detected in all Salmonella serotypes. Half of the serotypes possessed the ability of biofilm formation with varied adhesion strengths. These results revealed the high and unexpected prevalence of Salmonella serotypes in poultry feed with MDR and biofilm formation ability. BOXAIR and rep-PCR revealed a high diversity of Salmonella serotypes in feeds and subsequently indicated variation in the source of Salmonella spp. The unknown sources harboring high diversity of Salmonella serotypes indicated poor control, which could cause problems for feed manufacturing.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of feline hospitalization environment using a one-way mirror. 用单向镜评价猫住院环境。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145026

T Miyata, R Higuchi, K Yokobori, S Seki, K Ishioka

引用次数: 0

Correlation between clinical efficacy on pruritus and serum interleukin-31 levels in dogs with atopic dermatitis treated with lokivetmab. lokivetmab治疗特应性皮炎犬瘙痒临床疗效与血清白细胞介素-31水平的相关性

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145027

J R Calesso, V S Marques, O V de Carvalho, A P Costa-Val

{"title":"Correlation between clinical efficacy on pruritus and serum interleukin-31 levels in dogs with atopic dermatitis treated with lokivetmab.","authors":"J R Calesso, V S Marques, O V de Carvalho, A P Costa-Val","doi":"10.24425/pjvs.2023.145027","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145027","url":null,"abstract":"Studies on serum interleukin (IL)-31 levels in dogs with atopic dermatitis (AD) and their correlation with disease severity are limited. To the author's knowledge, there are no studies that measured serum IL-31 in dogs treated with lokivetmab injections, a selective inhibitor of this key cytokine in pruritus. The aim of the study was to evaluate serum IL-31 levels in dogs treated with lokivetmab and correlate it with the severity of canine atopic dermatitis using the pruritus visual analog scale (pVAS) and canine atopic dermatitis extent and severity index (CADESI-04). Ten client-owned dogs diagnosed with AD received two injections of lokivetmab four weeks apart. Disease severity was assessed using the pVAS and CADESI-04 scores before and after both injections. In addition, canine serum IL-31 levels were measured at the same moments. Serum IL-31 was detected in all dogs in the study. There was a significant reduction in pVAS scores and serum IL-31 after administrations. However, there was no difference in CADESI-04 scores, and there was no significant correlation between CADESI-04 scores and serum IL-31 in dogs diagnosed with AD. Nonetheless, a significant positive correlation was observed between the pVAS scores and serum IL-31 levels with lokivetmab therapy, which reinforces the role of IL-31 in the pathogenesis of pruritus in dogs with AD. The data presented here provide further evidence that IL-31 is directly involved in pruritus pathogenesis in dogs with AD. In addition, blocking IL-31 has a significant antipruritic effect, but has no influence on skin lesion severity and extension.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10099034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laparoscopic embryo transfer in pigs - comparison of different variants and efficiencies of the method. 猪的腹腔镜胚胎移植-不同变体和方法效率的比较。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145036

J Wieczorek, E Stodolak-Zych, K Okoń, J Koseniuk, M Bryła, J Jura, K Poniedziałek-Kempny, I Rajska, K Sobol, M Kotula Balak, M Chmurska-Gasowska

{"title":"Laparoscopic embryo transfer in pigs - comparison of different variants and efficiencies of the method.","authors":"J Wieczorek, E Stodolak-Zych, K Okoń, J Koseniuk, M Bryła, J Jura, K Poniedziałek-Kempny, I Rajska, K Sobol, M Kotula Balak, M Chmurska-Gasowska","doi":"10.24425/pjvs.2023.145036","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145036","url":null,"abstract":"The aim of the study was to develop a method of laparoscopic embryo transfer in pigs and to compare different variants of this method. Two catheter diameters (1.6 mm and 1.0 mm), the method and site of embryo deposition (oviduct or uterus), the embryo development stage (2 - 4 cell or blastocyst), the method for oviduct or uterus stabilization, the potential for cryopreserved embryo transfer, the developmental potential of the embryos after transfer to the oviduct, patomorphology of the oviduct after transfer and possible clinical complications were taken into consideration. Two studies compared two variants of transfer to the uterus, and five variants of transfer to the fallopian tube. The transfer of embryos by the infundibulum may be of limited use due to handling problems and very low efficiency (pregnancy was not achieved). Very low efficiency was shown after transfer of vitrified embryos. Transfer to the fallopian tube by puncture of the fallopian tube, regardless of the developmental stage of the embryo, is the recommended method of embryo transfer. The histopathological examination of the fallopian tube revealed possible changes within the puncture site. The numerous clinical complications observed did not affect the effectiveness of the method.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9739477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new strategy: high level expression and immunogenicity analysis of triplicate repeated multigenes in Mycoplasma hyopneumoniae. 新策略:低肺炎支原体三重复多基因的高表达及免疫原性分析。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-06-01 DOI: 10.24425/pjvs.2023.145034

J Li, G Wang

{"title":"A new strategy: high level expression and immunogenicity analysis of triplicate repeated multigenes in Mycoplasma hyopneumoniae.","authors":"J Li, G Wang","doi":"10.24425/pjvs.2023.145034","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145034","url":null,"abstract":"Highly immunogenic nucleotide fragments from 3 genes of Mycoplasma hyopneumoniae strain 232 were selected using information software technology. After repeating each fragment three times, a total of 9 nucleotide fragments were joined together to form a new nucleotide sequence called Mhp2321092bp. Mhp2321092bp was directly synthesized and cloned into a pET100 vector and expressed in Escherichia coli. After purification, the proteins were successfully validated by SDS-PAGE and Western blotting using mouse His-tag antibody and pig anti-Mhp serum. BALB/c mice were intraperitoneally injected with purified proteins in the high-dose (100 μg), medium-dose group (50 μg) and low-dose (10 μg) groups. Mice in each group were injected on day 1, day 8 and day 15 of feeding, respectively. Serum samples were collected from all mice on the day before immunization and on day 22 after immunization. The antibody level in the mouse serum was detected using western blotting using purified expressed proteins as antigens. IL-2, TNF-α and IFN-γ were simultaneously detected in the mouse serum by ELISA. The results showed that the 60 kDa protein was successfully expressed and reacted specifically with the specific serum Mhp His-Tag mouse monoclonal antibody and pig anti-Mhp serum. From day 0 to day 22 of immunization, IFN-γ increased from 269.52 to 467.74 pg/mL, IL-2 increased from 14.03 to 145.16 pg/mL, and TNF-α increased from 6.86 to 12.37 pg/mL. The IgG antibody in mice increased significantly from 0 day to day 22 after immunization. This study suggests that the expressed recombinant protein may serve as one of the novel vaccine candidates for Mhp.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0