Polish journal of veterinary sciences最新文献_第4页

Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season. 内源性一氧化碳(CO)作为分子生物钟的调节因子在猪和野猪杂种长、短昼期参与光传输的下丘脑结构中。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145008

P Gilun, M Koziorowska-Gilun, B Wąsowska, M Sowa-Kućma, K Kozioł, M Romerowicz-Misielak, W Kordan, M Koziorowski

{"title":"Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season.","authors":"P Gilun, M Koziorowska-Gilun, B Wąsowska, M Sowa-Kućma, K Kozioł, M Romerowicz-Misielak, W Kordan, M Koziorowski","doi":"10.24425/pjvs.2023.145008","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145008","url":null,"abstract":"Mature males of a wild boar-pig crossbreed, during the long and short day season, were used for the study which demonstrates that the chemical light carrier CO regulates the expression of biological clock genes in the hypothalamus via humoral pathways. Autologous blood with experimentally elevated concentrations of endogenous CO (using lamps with white light-emitting diodes) was infused into the ophthalmic venous sinus via the right dorsal nasal vein. Molecular biology methods: qPCR and Western Blot were used to determine the expression of genes and biological clock proteins. The results showed that elevated endogenous CO levels, through blood irradiation, induces changes in genes expression involved in the functioning of the main biological clock located in suprachiasmatic nuclei. Changes in the expression of the transcription factors Bmal1, Clock and Npas2 have a similar pattern in both structures, where a very large decrease in gene expression was shown after exposure to elevated endogenous CO levels. The changes in the gene expression of PER 1-2, CRY 1-2, and REV-ERB α-β and ROR β are not the same for both POA and DH hypothalamic structures, indicating that both structures respond differently to the humoral signal received. The results indicate that CO is a chemical light molecule whose production in an organism depends on the amount of light. An adequate amount of light is an essential factor for the proper functioning of the main biological clock.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of β-1,3/1,6 glucan dietary supplements on some immunological and hematological health markersin Siberian sturgeon (Acipenser baerii) infected with Aeromonas hydrophila. 饲粮中添加β-1,3/1,6葡聚糖对感染嗜水气单胞菌的西伯利亚鲟部分免疫和血液健康指标的影响

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145012

S Duman, A Şahan

{"title":"Effects of β-1,3/1,6 glucan dietary supplements on some immunological and hematological health markersin Siberian sturgeon (Acipenser baerii) infected with Aeromonas hydrophila.","authors":"S Duman, A Şahan","doi":"10.24425/pjvs.2023.145012","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145012","url":null,"abstract":"This experimental study aimed to investigate some hematological and immunological changes as a result of Aeromanas hydrophila infection in Siberian sturgeon (Acipenser baerii Brandt, 1869). Their feeds were supplemented with β-1,3/1,6 glucan at different ratios, 250 mg/kg (βG250); 500 mg/kg (βG500) and 750 mg/kg (βG750). To create an experimental infection, 4×106 cfu/ml Aeromonas hydrophila inoculum was intraperitoneally injected to fish. 0.1 ml intraperitoneal bacteria injection was given to the fish in 12 of 15 tanks, each consisting of 10 fish (the fish in the control group were not given bacteria). Considering the βG500 and βG750 group fish as positive (C+) and negative control (C-) groups in terms of hematological parameters, it was found that RBC (erythrocyte) and Hb (hemoglobin) values, as well as RBC indices (MCV, MCHC, MCH) significantly increased. The immunological parameters, including WBC (leukocyte), leukocyte cell percentages (lymphocyte, monocyte, neutrophil, eosinophil), as well as cytokines, including IL-1β, IL-6, IL-8, and TNF-α values showed similar increases in the βG500 and βG750 groups. It was found that the addition of 500 and 750 mg/kg doses of β-1,3/1,6 glucan to the feed stimulated non-specific immunity of fish against bacterial agents and/or septicemic diseases and beta glucan at this dosage range was determined to be ideal for fish health and that it may be a herbal immunostimulant that can be an alternative to many medicaments.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Review and future perspectives on the integration characteristics for equine lentivirus in the host genome. 马慢病毒在宿主基因组整合特性的研究进展与展望。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145019

Y-Y Yu, M-S Xu, H Liang, H-Y Wang, C-Q Yu, Q Liu

{"title":"Review and future perspectives on the integration characteristics for equine lentivirus in the host genome.","authors":"Y-Y Yu, M-S Xu, H Liang, H-Y Wang, C-Q Yu, Q Liu","doi":"10.24425/pjvs.2023.145019","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145019","url":null,"abstract":"Despite over 40 years of research on the human immunodeficiency virus type 1 (HIV-1) vaccine, we still lack a considerable progress. Equine infectious anemia virus (EIAV) is a lentivirus in the Retroviridae family, akin to HIV-1 in genome structure and antigenicity. EIA is an important infectious disease in equids, characterized by anemia, persistent infection, and repeated fevers. The EIAV attenuated vaccine in China is the only lentiviral vaccine used on a large scale. Elucidating the mechanism of waning and induction of protective immunity from this attenuated vaccine strain will provide a critical theoretical basis and reference point for vaccine research, particularly in the development of lentivirus vaccines, with far-reaching scientific value and social significance. In this paper, we summarize the information related to EIAV integration site selection, particularly for the Chinese EIAV attenuated vaccine strains on the equine genome. This may improve our mechanistic understanding of EIAV virulence reduction at the host genome level. The obtained data may help elucidate the biological characteristics of EIAV, particularly the Chinese attenuated EIAV vaccine strain, and provide valuable information regarding retroviral infections, particularly lentiviral infection and associated therapeutic vectors.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9567322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of tylosin and tilmicosin residues in meat by high-performance liquid chromatography method. 高效液相色谱法测定肉类中泰乐素和替尔米科星的残留。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145005

M Gürel Yücel, H Seçilmiş, F Taşçı

{"title":"Investigation of tylosin and tilmicosin residues in meat by high-performance liquid chromatography method.","authors":"M Gürel Yücel, H Seçilmiş, F Taşçı","doi":"10.24425/pjvs.2023.145005","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145005","url":null,"abstract":"In this study, the presence and level of macrolide group antibiotics (tylosin and tilmicosin) were analyzed by the High-Performance Liquid Chromatography (HPLC) method in a total of 126 raw meat samples, including 42 chicken breast and 84 beef neck, available for consumption in the Burdur province (Turkey). The method demonstrated good linearity (R2 > 0.999) over the assayed concentration range (0.10-10 μg/mL). Intra-day and inter-day recoveries were used to express the accuracy of the method at three different levels of 0.5, 1, 2.5 μg/mL. Intraday recoveries and relative standard deviation values ranged from 97.270 (0.054)% to 98.643 (0.061)%, and inter-day recoveries and relative standard deviation values ranged from 97.057 (0.070)% to 98.197(0.042)% for tylosin. Intraday recoveries and relative standard deviation values ranged from 96.360 (0.065)% to 98.153 (0.046)%, and inter-day recoveries and relative standard deviation values ranged from 96.050 (0.058)% to 97.053 (0.096)% for tilmicosin. The limit of detection (LOD) value was calculated as 0.473 μg/kg for tylosin, and 0.481 μg/kg for tilmicosin; the limit of quantification (LOQ) value was calculated as 1.561 μg/kg for tylosin, and 1.587 μg/kg for tilmicosin. In general, tylosin and tilmicosin were determined in the range of 8-256 μg/kg and 30-447 μg/kg, respectively, in chicken breast meat samples; also, they were detected in the range of 36-1209 μg/kg and 30-1102 μg/kg, respectively, in beef neck meat samples. It was also found that the residues of tylosin and tilmicosin in chicken and beef meats from the market were at a much higher level than the acceptable limits specified in the regulations. This creates serious problems in terms of the ecosystem, food technology, and public health, and causes significant economic losses.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan. 巴基斯坦禽源产广谱β -内酰胺酶(ESBL)的肠沙门氏菌和大肠杆菌的发病率和分子特征

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145006

M A Saeed, U Waheed, S Ehtisham-Ul-Haque, A U Khan, M Kashif, M F Qamar, A Ghafoor, M Saqlain, J Asghar

{"title":"Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan.","authors":"M A Saeed, U Waheed, S Ehtisham-Ul-Haque, A U Khan, M Kashif, M F Qamar, A Ghafoor, M Saqlain, J Asghar","doi":"10.24425/pjvs.2023.145006","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145006","url":null,"abstract":"Members of Enterobacteriaceae are known to produce extended-spectrum beta-lactamases (ESBL) which hydrolyze the beta-lactam group of antibiotics. The existence of ESBL-producing Salmonella enterica (S. enterica) and Escherichia coli (E. coli) harbored by urban avifauna was investigated in this study. Dropping samples (n= 180) were collected from six different bird species in the district Jhang, Punjab province, Pakistan. Isolation and identification of ESBL isolates were made by using cefotaxime- (4 mg/L) supplemented MacConkey agar and double disc synergy test (DDST). Polymerase chain reaction (PCR) was performed for the detection of four different ESBL genes including blaCTX-M, blaTEM, blaSHV and blaOXA. A total of 42.69% isolates were confirmed as ESBL via DDST including 30.64% S. enterica and 49.54% E. coli. The incidence of ESBL S. enterica and ESBL E. coli was found highest in egret (Ardea alba) and pigeon (Columba livia) as 64.28% and 78.95%, respectively. The blaCTX-M gene was detected in 57.89% and 64.81% of isolates of S. enterica and E. coli, respectively. Among other genes in S. enterica and E. coli, blaTEM (21.05%, 20.4%); blaSHV (15.78%, 9.26%), and blaOXA (5.26%, 5.56%) were detected, respectively. All of the tested isolates were found resistant to at least one of the thirteen antimicrobial agents except meropenem. To the best of our knowledge, this is the first study reporting the incidence and genetic diversity of ESBL bacteria associated with urban avifauna in Pakistan. The urban avifauna can serve as a potential subject of bio-surveillance to monitor the emergence of antimicrobial-resistant bacteria.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of the anti-mycobacterial activity and composition of Carlina acaulis L. root extracts. Carlina acaulis L.根提取物抗分枝杆菌活性及成分评价。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145007

K Puk, J Wawrzykowski, L Guz

{"title":"Evaluation of the anti-mycobacterial activity and composition of Carlina acaulis L. root extracts.","authors":"K Puk, J Wawrzykowski, L Guz","doi":"10.24425/pjvs.2023.145007","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145007","url":null,"abstract":"Nontuberculous mycobacteria (NTM) have recently emerged as important bacterial pathogens of animals and humans. Of particular concern is the high level of antimicrobial resistance displayed by these organisms, which complicates treatment and potential successful outcomes. Here, we evaluated the potential of Carlina acaulis L. as a source of novel anti-mycobacterial agents. Our goal was to measure the activity of aqueous, ethanol, and chloroform C. acaulis root extracts against 99 NTM strains. GC-MS spectroscopy analyses were performed to deliver qualitative and quantitative data on the composition of C. acaulis extract. In our study, we have shown for the first time the activity of C. acaulis extracts against NTM. The highest activity was exhibited by the chloroform extract, which inhibited the growth of more than 90% of the strains at the dose of 100 μg/mL (MIC90 = 100 μg/mL). The results of the GC-MS analysis of the C. acaulis chloroform extract contributed to the identification of 37 compounds, with carlina oxide as the most representative compound (69.52%) followed by 3,4-dihydro-2H-phenanthren- -1-one (6.54%) and stigmast-5-en-3-ol (4.14%). Our results indicate that C. acaulis chloroform and ethanol extracts have potential for treatment of NTM infections and that this plant contains anti-mycobacterial compounds.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9192662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thyroid evaluation in suspicious hypothyroid adult dogs before and after treatment. 可疑甲状腺功能减退犬治疗前后甲状腺功能评估。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145011

O Bucalo, K Satué, P Medica, C Cravana, E Fazio

{"title":"Thyroid evaluation in suspicious hypothyroid adult dogs before and after treatment.","authors":"O Bucalo, K Satué, P Medica, C Cravana, E Fazio","doi":"10.24425/pjvs.2023.145011","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145011","url":null,"abstract":"The purpose of this study was to measure circulating TSH, T4 and fT4 concentrations in dogs submitted to a clinical visit for general symptoms (weight gain, polyuria and polydipsia, changes in hair coat). Twenty-eight dogs, 14 cross-breed and 14 purebreds (Golden Retriever, Labrador, Doberman), of both sexes (14 males and 14 females), aged 8 to 14 years, were assessed. No significant differences of circulating TSH, T4 , fT4 concentrations between the baseline and after therapeutic treatment nor between intact and neutered females were observed. Compared to baseline values, intact males showed higher TSH concentrations (p⟨0.01), and castrated males lower TSH concentrations (p⟨0.01) after therapeutic treatment. Compared to intact males, castrated males showed baseline TSH concentrations higher (p⟨0.01), but lower (p⟨0.01) after therapeutic treatment. No significant differences of T4 and fT4 concentrations between baseline conditions and after therapeutic treatment, nor between intact and castrated males, were observed. The experimental sample considered in this study falls within that casuistry involving elevated TSH concentrations but low serum T4 and fT4 concentrations or close to the minimum physiological cut-off, in which the common clinical signs suggestive of hypothyroidism was, essentially, overweight and neglected appearance of the hair.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SNP panel for evaluation of genetic variability and relationship in roe deer (Capreolus capreolus). 用于评估狍(Capreolus Capreolus)遗传变异和亲缘关系的SNP面板。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145004

K Oleński, D Zalewski, S Kamiński

{"title":"SNP panel for evaluation of genetic variability and relationship in roe deer (Capreolus capreolus).","authors":"K Oleński, D Zalewski, S Kamiński","doi":"10.24425/pjvs.2023.145004","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145004","url":null,"abstract":"Blood samples from forty-six roe deer ( Capreolus capreolus) acquired during officially approved hunting in six hunting divisions throughout Poland were used to isolate the genomic DNA. All individuals were genotyped by MD_Bovine BeadChip (Illumina) for 46.750 Single Nucleotide Polymorphism (SNP) markers. SNPs of inappropriate clusters, with a marker call rate lower than 90% and with a minor allele frequency (MAF) lower than 0.01, located on sex chromosomes and mitochondrial DNA, were removed. Altogether, 21.033 SNP markers were included for further analysis. Observed and expected heterozygosity amounted to 0.098 and 0.119, respectively. Among 21.033 markers, a panel of 148 SNPs were selected for relationship analysis. They were unlinked and had a MAF higher than 0.2. This set of SNPs showed a probability of parentage exclusion of 1.29x10 -6 and 2.37x10 -19 for one, and two known parents, respectively. The probability of identity was estimated at 1.8x10 -40. The probabilities obtained in this study are sufficient for the monitoring and effective management of the genetic diversity of roe deer in Poland and is a cost-effective complementary tool for forensic applications.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological studies of the round goby (Neogobius melanostomus) from the Puck Bay, southern Baltic Sea. 波罗的海南部帕克湾圆虾虎鱼(Neogobius melanostomus)的微生物学研究。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145017

K Nadolna-Ałtyn, A Pękala-Safińska, P Balazy, G Siedlewicz, E Paździor, J Pawlak, I Psuty

引用次数: 0

The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen. 木犀草素对冷冻兔精液精子学参数、细胞凋亡及氧化应激率的影响。

IF 0.8 4区农林科学

Polish journal of veterinary sciences Pub Date : 2023-03-01 DOI: 10.24425/pjvs.2023.145010

S A Akarsu, T C Acısu, I H Güngör, A Çakır Cihangiroğlu, R H Koca, G Türk, M Sönmez, S Gür, F Fırat, H E Esmer Duruel

{"title":"The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen.","authors":"S A Akarsu, T C Acısu, I H Güngör, A Çakır Cihangiroğlu, R H Koca, G Türk, M Sönmez, S Gür, F Fırat, H E Esmer Duruel","doi":"10.24425/pjvs.2023.145010","DOIUrl":"https://doi.org/10.24425/pjvs.2023.145010","url":null,"abstract":"The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 µM, LUT 50 µM, LUT 100 µM and LUT 200 µM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 106 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 µM compared to the other groups (p⟨0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 µM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H2 O2 percentage was found to be lower in all experimental groups compared to the control group (p⟨0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.","PeriodicalId":20366,"journal":{"name":"Polish journal of veterinary sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0