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Histony łącznikowe H1 w chorobach człowieka 人类疾病中的 H1 连接组蛋白
Postępy Biochemii Pub Date : 2024-08-09 DOI: 10.18388/pb.2021_561
Adrianna Żukowska, Joanna Perła-Kaján
{"title":"Histony łącznikowe H1 w chorobach człowieka","authors":"Adrianna Żukowska, Joanna Perła-Kaján","doi":"10.18388/pb.2021_561","DOIUrl":"https://doi.org/10.18388/pb.2021_561","url":null,"abstract":"Linker histones (H1) are basic proteins that are part of the nucleosome structure in the cell nucleus and are involved in the packaging of genetic material and the regulation of gene expression. As research progressed, it was discovered that linker histones constitute the largest group of histones in terms of variants found in humans. Even though the H1 variants differ slightly in the primary structure, they can perform different functions, undergo multiple post-translational modifications and differ in cellular localization. In addition to the nucleus, histones H1 can occur in the cytoplasm, on the cell surface and in the intercellular space. In these places, they play a supporting role for the immune system and act as signaling molecules. Changes in the levels of histones and their post-translational modifications have been associated with many human diseases and it is postulated that some of them may serve as biomarkers or therapeutic targets.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141924361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Historia Instytutu Chemii Bioorganicznej Polskiej Akademii Nauk w Poznaniu 波兹南波兰科学院生物有机化学研究所的历史
Postępy Biochemii Pub Date : 2024-06-05 DOI: 10.18388/pb.2021_556
L. Handschuh, Marek Figlerowicz, Agnieszka Konrad, Edyta Kościańska
{"title":"Historia Instytutu Chemii Bioorganicznej Polskiej Akademii Nauk w Poznaniu","authors":"L. Handschuh, Marek Figlerowicz, Agnieszka Konrad, Edyta Kościańska","doi":"10.18388/pb.2021_556","DOIUrl":"https://doi.org/10.18388/pb.2021_556","url":null,"abstract":"W 2023 roku Instytut Chemii Bioorganicznej Polskiej Akademii Nauk w Poznaniu (ICHB PAN) obchodził 35-lecie istnienia. Dodatkowo afiliowane przy Instytucie Poznańskie Centrum Superkomputerowo-Sieciowe (PCSS) świętowało 30-lecie powołania przez Kolegium Rektorów miasta Poznania. Obchody podwójnego jubileuszu uświetniła międzynarodowa konferencja naukowa Understand and describe life, która odbyła się w stolicy Wielkopolski w dniach 14-15 listopada 2023 r. Przybyło na nią wielu znamienitych gości z kraju i zagranicy. Spotkanie było okazją do wymiany myśli i dyskusji na temat różnych aspektów życia, od molekularnego po populacyjny i wirtualny.\u0000Jubileusz to z jednej strony czas na podsumowanie tego, co udało się dokonać, z drugiej czas na refleksję na temat kierunków przyszłych działań. Niezwykła historia Instytutu, którą przybliżamy poniżej, jest przykładem łączenia naukowej pasji z wizją, determinacją i odwagą do podejmowania ambitnych wyzwań.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141383785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-analyte responsive luminescent probes for the detection of biochemical targets in cell models 用于检测细胞模型中生化目标的多分析物反应发光探针
Postępy Biochemii Pub Date : 2024-06-05 DOI: 10.18388/pb.2021_538
Anna Wychowaniec, Francesca Canyelles i Font, Masroor Ahmed Khan, MIchał Gładysz, Dorota Kwiatek, Jacek L Kolanowski
{"title":"Multi-analyte responsive luminescent probes for the detection of biochemical targets in cell models","authors":"Anna Wychowaniec, Francesca Canyelles i Font, Masroor Ahmed Khan, MIchał Gładysz, Dorota Kwiatek, Jacek L Kolanowski","doi":"10.18388/pb.2021_538","DOIUrl":"https://doi.org/10.18388/pb.2021_538","url":null,"abstract":"Luminescence has found wide application in biology, biotechnology and medicine. Particularly, fluorescent and bioluminescent probes allow visualization of molecular targets at the cellular level and even macromolecules or single small-molecule analytes. Among the most reliable tools for visualization of molecular targets are so-called responsive probes, which change the intensity and colour of the emitted signal after interaction with a molecular target. The majority of such probes allow detection of a single analyte. Meanwhile, most of the processes in the human body involve multiple elements. To better understand these mechanisms, it is possible to use several responsive probes simultaneously. However, this poses a risk of their different uptake by cells or different metabolism. In order to provide a more reliable response, so-called multi-analyte and multi-responsive probes are being developed. Examples of such probes will be discussed in the article, divided based on their response mechanism and detected changes within the cell.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141385132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metody spektrometrii mas oparte o dane (DDA) oraz metody niezależne od danych (DIA) wykorzystywane w analizie materiału biologicznego 用于分析生物材料的数据驱动（DDA）和数据无关（DIA）质谱方法
Postępy Biochemii Pub Date : 2024-06-05 DOI: 10.18388/pb.2021_535
Marta Nolka-Szaszner, Aleksander Strugała, Łukasz Marczak
{"title":"Metody spektrometrii mas oparte o dane (DDA) oraz metody niezależne od danych (DIA) wykorzystywane w analizie materiału biologicznego","authors":"Marta Nolka-Szaszner, Aleksander Strugała, Łukasz Marczak","doi":"10.18388/pb.2021_535","DOIUrl":"https://doi.org/10.18388/pb.2021_535","url":null,"abstract":"Mass spectrometry is an important tool in proteomic, metabolomic and lipidomic analysis. To fully use its potential, it is crucial to select and configure the appropriate analytical approach. For untargeted research, there are two main strategies available: data-dependent analysis (DDA) and data-independent analysis (DIA). Both methods differ in the way the analysis is carried out and in the degree of coverage of the obtained data, which is why each of them can be used in various types of research. The DDA method is based on continuous scanning of the analyzed ions, as a result of which the precursors with the highest intensity are fragmented in the MS2 mode. On the other hand, DIA, due to the use of combined ranges of precursor ion isolation, allows for a deeper analysis of the analyzed compounds. Both approaches also have modifications that improve their operation and enable obtaining more valuable data. Methods combining both techniques are also appearing on the horizon, such as DDIA, which uses the advantages of both methods, opening new analytical possibilities.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141384220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cruciferous vegetables In Hashimoto’s disease diet 桥本氏病饮食中的十字花科蔬菜
Postępy Biochemii Pub Date : 2024-06-05 DOI: 10.18388/pb.2021_547
Dominika Dobiecka, Justyna Moskwa, R. Markiewicz-Żukowska, K. Socha, S. Naliwajko
{"title":"Cruciferous vegetables In Hashimoto’s disease diet","authors":"Dominika Dobiecka, Justyna Moskwa, R. Markiewicz-Żukowska, K. Socha, S. Naliwajko","doi":"10.18388/pb.2021_547","DOIUrl":"https://doi.org/10.18388/pb.2021_547","url":null,"abstract":"Thyroid is an endocrine gland that is responsible for producing and releasing two hormones: triiodothyronine (T3) and thyroxine (T4). Hypothyroidism as the disorder happens when the synthesis of those hormones is impaired. It is most commonly caused by the chronic autoimmune inflammation of the thyroid, referred to as Hashimoto’s disease. In this case, apart from the pharmacological treatment, diet does matter a great deal, too. It is extremely important to provide the body with all the essential nutrients. Exclusion of products that may interfere with the uptake of iodine - the trace element that is indispensable for the thyroid gland hormone synthesis, seems to be crucial, too. They most of all include cruciferous vegetables containing goitrogens (goitrogenic substances). This review aimed to collect and summarize the available scientific data on the safety of the cruciferous vegetable consumption within the context of its impact upon the thyroid function.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141386274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mikrokalorymetria jako narzędzie w badaniach kinetyki enzymatycznej 作为酶动力学研究工具的微量热仪
Postępy Biochemii Pub Date : 2024-06-05 DOI: 10.18388/pb.2021_529
Joanna Śliwiak, A. Urbanowicz
{"title":"Mikrokalorymetria jako narzędzie w badaniach kinetyki enzymatycznej","authors":"Joanna Śliwiak, A. Urbanowicz","doi":"10.18388/pb.2021_529","DOIUrl":"https://doi.org/10.18388/pb.2021_529","url":null,"abstract":"Enzymes, as biocatalysts, are an important target of many therapies and are also of great industrial importance, which is why repeatable and accurate parameterization of enzymatic catalysis is very important. The most popular spectrophotometric detection method in enzymology, despite its low cost and speed, often cannot be used directly due to the inappropriate spectral properties of substrates and products. It is then necessary to use auxiliary enzymes, chemical modification of substrates or post-reaction analysis, which may increase the cost of measurement, extend its time or affect the accuracy. Isothermal titration calorimetry is a method widely used mainly for the characterization of inter-molecular interactions, however, its use in enzyme kinetics is gaining more and more recognition due to the direct measurement of the reaction rate using the universal parameter of heat, high sensitivity and low reagent consumption. This work discusses two strategies for conducting a kinetic calorimetric experiment and their applications.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141382228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sekwencjonowanie transkryptomu pojedynczych komórek: droga do odkrywania bioróżnorodności komórkowej 单细胞转录组测序：发现细胞生物多样性的途径
Postępy Biochemii Pub Date : 2024-05-10 DOI: 10.18388/pb.2021_532
Anna Samelak-Czajka, Małgorzata Marszałek-Zeńczak, Paulina Jackowiak
{"title":"Sekwencjonowanie transkryptomu pojedynczych komórek: droga do odkrywania bioróżnorodności komórkowej","authors":"Anna Samelak-Czajka, Małgorzata Marszałek-Zeńczak, Paulina Jackowiak","doi":"10.18388/pb.2021_532","DOIUrl":"https://doi.org/10.18388/pb.2021_532","url":null,"abstract":"Single-cell transcriptomics (scRNA-Seq) is a breakthrough technology that has opened the way to characterizing gene expression with unprecedented resolution. It has enabled the discovery of the cellular diversity of organisms and tracing their developmental processes. A range of technological solutions have been developed to allow analysis of tens of thousands to even a million cells in a single experiment, as well as an extensive set of tools for bioinformatics analysis of the generated data. The wealth of information provided by scRNA-Seq and the possibility of using this method to study cells, organoids, tissues and even entire organisms determine its wide range of applications. In this paper, we present the experimental and computational parts of the scRNA-Seq procedure, as well as the most important applications of this technology in biomedicine, developmental biology and plant biology.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140992140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ion Moblity Mass Spectrometry in Multi-omics Studies 离子迁移质谱法在多组学研究中的应用
Postępy Biochemii Pub Date : 2024-05-10 DOI: 10.18388/pb.2021_530
Daniel Fochtman, Anna Wojakowska, Łukasz Marczak
{"title":"Ion Moblity Mass Spectrometry in Multi-omics Studies","authors":"Daniel Fochtman, Anna Wojakowska, Łukasz Marczak","doi":"10.18388/pb.2021_530","DOIUrl":"https://doi.org/10.18388/pb.2021_530","url":null,"abstract":"Mass spectrometry (MS) as an analytical technique enables the identification and quantitative determination of proteins, metabolites, or lipids in a studied sample. However, this method has limitations regarding the number of molecules that can be identified at a given time. To increase the number of identifications, the application of ion mobility spectrometry (IMS) can be employed. This technique allows the separation of ions based on their mobility while traversing the analyser in a gradient of an electromagnetic field and opposing gas pressure. The separation is performed in conjunction with MS analysis, adding another dimension to the analysis, resulting in a significant improvement in the number of identified compounds and a reduction in noise. Alternatively, while maintaining the same number of identifications, analysis can be performed in a shorter time period. It is crucial to pay special attention to the type of IMS analyser used, as its specific implementation dictates further stages of analysis and ion detection capabilities.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140992112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rola i wykorzystanie badań wysokoprzepustowych w poszukiwaniu związków chemicznych o działaniu terapeutycznym 高通量检测在寻找具有治疗活性的化合物中的作用和应用
Postępy Biochemii Pub Date : 2024-05-10 DOI: 10.18388/pb.2021_528
Natalia Karczewska, Monika Pyc, Krzysztof Żukowski, Joanna Kosman, Dorota Kwiatek, J. Kolanowski
{"title":"Rola i wykorzystanie badań wysokoprzepustowych w poszukiwaniu związków chemicznych o działaniu terapeutycznym","authors":"Natalia Karczewska, Monika Pyc, Krzysztof Żukowski, Joanna Kosman, Dorota Kwiatek, J. Kolanowski","doi":"10.18388/pb.2021_528","DOIUrl":"https://doi.org/10.18388/pb.2021_528","url":null,"abstract":"With the development of medicine and the aging population, the demand\u0000for more effective therapies is escalating. A tool that facilitates the discovery and\u0000introduction of new therapeutic drugs is High-Throughput Screening (HTS). These tests, consisting of a wide set of various assays, allow testing hundreds of thousands of compounds in a short period of time. The aim is to accurately identify active compounds that could become potential therapeutic candidates in the pharmaceutical industry. HTS is the first step in the quest for potential drugs, therefore it is one of the crucial tests that determine whether a particular drug candidate will be discovered. In this review, different stages of high-throughput screening studies will be described, as well as methods utilized in these studies. The necessary steps in the optimization of these tests, selection of equipment, automation  and key quality control parameters for reliably performed screening will also be presented.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140991090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lipazy roślinne – struktura molekularna, rola w ontogenezie oraz potencjał biotechnologiczny 植物脂肪酶--分子结构、在本体发育中的作用和生物技术潜力
Postępy Biochemii Pub Date : 2024-05-10 DOI: 10.18388/pb.2021_539
Alan Stafiej, Karolina Wleklik, Marta Przybylak, Sławomir Borek
{"title":"Lipazy roślinne – struktura molekularna, rola w ontogenezie oraz potencjał biotechnologiczny","authors":"Alan Stafiej, Karolina Wleklik, Marta Przybylak, Sławomir Borek","doi":"10.18388/pb.2021_539","DOIUrl":"https://doi.org/10.18388/pb.2021_539","url":null,"abstract":"Lipases are enzymes commonly found in microorganisms, fungi, plants and animals. Their main function in cell metabolism is the hydrolysis (lipolysis) of ester bonds between fatty acids and glycerol in mono-, di- and triacylglycerols. In plants, lipases play an important role in ontogeny, participating in both vegetative development and generative stages. These enzymes may also be a component of plant responses to biotic and abiotic stresses. Based on the similarity of the amino acid sequence and vacuolar localization of some plant lipases to yeast Atg15, we present a hypothesis about the participation of lipases in autophagy (precisely, in the degradation of the autophagic body) in plants. Despite the narrow substrate specificity and the type of reactions catalysed in cells, lipases find numerous biotechnological applications. The physicochemical features of lipases, which determine, for example, wide substrate specificity in vitro or high stability in a wide range of pH and temperature, make these enzymes the subject of applied research, and plant lipases show an increasing potential in this area of science and industry.","PeriodicalId":20341,"journal":{"name":"Postępy Biochemii","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140993563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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