高通量检测在寻找具有治疗活性的化合物中的作用和应用

Natalia Karczewska, Monika Pyc, Krzysztof Żukowski, Joanna Kosman, Dorota Kwiatek, J. Kolanowski
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引用次数: 0

摘要

随着医学的发展和人口老龄化的加剧,人们对更有效疗法的需求也在不断增长。高通量筛选(HTS)是促进发现和推出新治疗药物的一种工具。这些试验由多种不同的检测方法组成,可在短时间内测试成千上万种化合物。这样做的目的是为了准确识别出可能成为制药业潜在治疗候选药物的活性化合物。HTS 是寻找潜在药物的第一步,因此是决定特定候选药物能否被发现的关键测试之一。本综述将介绍高通量筛选研究的不同阶段以及在这些研究中使用的方法。此外,还将介绍优化这些测试的必要步骤、设备选择、自动化和关键质量控制参数,以便可靠地进行筛选。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rola i wykorzystanie badań wysokoprzepustowych w poszukiwaniu związków chemicznych o działaniu terapeutycznym
With the development of medicine and the aging population, the demand for more effective therapies is escalating. A tool that facilitates the discovery and introduction of new therapeutic drugs is High-Throughput Screening (HTS). These tests, consisting of a wide set of various assays, allow testing hundreds of thousands of compounds in a short period of time. The aim is to accurately identify active compounds that could become potential therapeutic candidates in the pharmaceutical industry. HTS is the first step in the quest for potential drugs, therefore it is one of the crucial tests that determine whether a particular drug candidate will be discovered. In this review, different stages of high-throughput screening studies will be described, as well as methods utilized in these studies. The necessary steps in the optimization of these tests, selection of equipment, automation  and key quality control parameters for reliably performed screening will also be presented.
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