Plant PhenomicsPub Date : 2023-11-21DOI: 10.34133/plantphenomics.0124
Yuanning Zhai, Lei Zhou, Hengnian Qi, Pan Gao, Chu Zhang
{"title":"Application of visible/near-infrared spectroscopy and hyperspectral imaging with machine learning for high-throughput plant heavy metal stress phenotyping: a review","authors":"Yuanning Zhai, Lei Zhou, Hengnian Qi, Pan Gao, Chu Zhang","doi":"10.34133/plantphenomics.0124","DOIUrl":"https://doi.org/10.34133/plantphenomics.0124","url":null,"abstract":"","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-13DOI: 10.34133/plantphenomics.0118
Xiujuan Wang, Jing Hua, Mengzhen Kang, Haoyu Wang, Philippe de Reffye
{"title":"Functional-Structural Plant Model 'GreenLab': A State-of-the-Art Review","authors":"Xiujuan Wang, Jing Hua, Mengzhen Kang, Haoyu Wang, Philippe de Reffye","doi":"10.34133/plantphenomics.0118","DOIUrl":"https://doi.org/10.34133/plantphenomics.0118","url":null,"abstract":"","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136282651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-10eCollection Date: 2023-01-01DOI: 10.34133/plantphenomics.0112
Shamprikta Mehreen, Hervé Goëau, Pierre Bonnet, Sophie Chau, Julien Champ, Alexis Joly
{"title":"Estimating Compositions and Nutritional Values of Seed Mixes Based on Vision Transformers.","authors":"Shamprikta Mehreen, Hervé Goëau, Pierre Bonnet, Sophie Chau, Julien Champ, Alexis Joly","doi":"10.34133/plantphenomics.0112","DOIUrl":"10.34133/plantphenomics.0112","url":null,"abstract":"<p><p>The cultivation of seed mixtures for local pastures is a traditional mixed cropping technique of cereals and legumes for producing, at a low production cost, a balanced animal feed in energy and protein in livestock systems. By considerably improving the autonomy and safety of agricultural systems, as well as reducing their impact on the environment, it is a type of crop that responds favorably to both the evolution of the European regulations on the use of phytosanitary products and the expectations of consumers who wish to increase their consumption of organic products. However, farmers find it difficult to adopt it because cereals and legumes do not ripen synchronously and the harvested seeds are heterogeneous, making it more difficult to assess their nutritional value. Many efforts therefore remain to be made to acquire and aggregate technical and economical references to evaluate to what extent the cultivation of seed mixtures could positively contribute to securing and reducing the costs of herd feeding. The work presented in this paper proposes new Artificial Intelligence techniques that could be transferred to an online or smartphone application to automatically estimate the nutritional value of harvested seed mixes to help farmers better manage the yield and thus engage them to promote and contribute to a better knowledge of this type of cultivation. For this purpose, an original open image dataset has been built containing 4,749 images of seed mixes, covering 11 seed varieties, with which 2 types of recent deep learning models have been trained. The results highlight the potential of this method and show that the best-performing model is a recent state-of-the-art vision transformer pre-trained with self-supervision (Bidirectional Encoder representation from Image Transformer). It allows an estimation of the nutritional value of seed mixtures with a coefficient of determination <i>R</i><sup>2</sup> score of 0.91, which demonstrates the interest of this type of approach, for its possible use on a large scale.</p>","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-10eCollection Date: 2023-01-01DOI: 10.34133/plantphenomics.0095
Min Li, Pengcheng Hu, Di He, Bangyou Zheng, Yan Guo, Yushan Wu, Tao Duan
{"title":"Quantification of the Cumulative Shading Capacity in a Maize-Soybean Intercropping System Using an Unmanned Aerial Vehicle.","authors":"Min Li, Pengcheng Hu, Di He, Bangyou Zheng, Yan Guo, Yushan Wu, Tao Duan","doi":"10.34133/plantphenomics.0095","DOIUrl":"10.34133/plantphenomics.0095","url":null,"abstract":"<p><p>In intercropping systems, higher crops block direct radiation, resulting in inevitable shading on the lower crops. Cumulative shading capacity (<i>CSC</i>), defined as the amount of direct radiation shaded by higher crops during a growth period, affects the light interception and radiation use efficiency of crops. Previous studies investigated the light interception and distribution of intercropping. However, how to directly quantify the <i>CSC</i> and its inter-row heterogeneity is still unclear. Considering the canopy height differences (<i>H<sub>ms</sub></i>, obtained using an unmanned aerial vehicle) and solar position, we developed a shading capacity model (SCM) to quantify the shading on soybean in maize-soybean intercropping systems. Our results indicated that the southernmost row of soybean had the highest shading proportion, with variations observed among treatments composed of strip configurations and plant densities (ranging from 52.44% to 57.44%). The maximum overall <i>CSC</i> in our treatments reached 123.77 MJ m<sup>-2</sup>. There was a quantitative relationship between <i>CSC</i> and the soybean canopy height increment (<i>y</i> = 3.61 × 10<sup>-2</sup>×ln(<i>x</i>)+6.80 × 10<sup>-1</sup>, <i>P</i> < 0.001). Assuming that the growth status of maize and soybean was consistent under different planting directions and latitudes, we evaluated the effects of factors (i.e., canopy height difference, latitude, and planting direction) on shading to provide insights for optimizing intercropping planting patterns. The simulation showed that increasing canopy height differences and latitude led to increased shading, and the planting direction with the least shading was about 90° to 120° at the experimental site. The newly proposed SCM offers a quantitative approach for better understanding shading in intercropping systems.</p>","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10637764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41715527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-07DOI: 10.34133/plantphenomics.0120
Laetitia Lemiere, Marc Jaeger, Marie Gosme, Gérard Subsol
{"title":"Combinatorial maps, a new framework to model agroforestry systems","authors":"Laetitia Lemiere, Marc Jaeger, Marie Gosme, Gérard Subsol","doi":"10.34133/plantphenomics.0120","DOIUrl":"https://doi.org/10.34133/plantphenomics.0120","url":null,"abstract":"","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135540467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-07DOI: 10.34133/plantphenomics.0119
Tingting Wu, Peng Shen, Jianlong Dai, Yuntao Ma, Yi Feng
{"title":"A Pathway to Assess Genetic Variation of Wheat Germplasm by Multi-dimensional Traits with Digital Images","authors":"Tingting Wu, Peng Shen, Jianlong Dai, Yuntao Ma, Yi Feng","doi":"10.34133/plantphenomics.0119","DOIUrl":"https://doi.org/10.34133/plantphenomics.0119","url":null,"abstract":"In this paper, a new pathway was proposed to assess the germplasm genetic variation by multidimensional traits of wheat seeds generated from digital images. A machine vision platform was first established to reconstruct wheat germplasm 3D model from omnidirectional image sequences of wheat seeds. Then, multidimensional traits were conducted from the wheat germplasm 3D model, including seed length, width, thickness, surface area, volume, maximum projection area, roundness, and 2 new defined traits called cardioid-derived area and the index of adjustment (J index). To assess genetic variation of wheat germplasm, phenotypic coefficients of variation (PCVs), analysis of variance (ANOVA), clustering, and the defined genetic variation factor (GVF) were calculated using the extracted morphological traits of 15 wheat accessions comprising 13 offspring and 2 parents. The measurement accuracy of 3D reconstruction model is demonstrated by the correlation coefficient (R) and root mean square errors (RMSEs). Results of PCVs among all the traits show importance of multidimensional traits, as seed volume (22.4%), cardioid-derived area (16.97%), and maximum projection area (14.67%). ANOVA shows a highly significance difference among all accessions. The results of GVF innovatively reflect the connection between genotypic variance and phenotypic traits from parents to offspring. Our results confirmed that extracting multidimensional traits from digital images is a promising high-throughput and cost-efficient pathway that can be included as a valuable approach in genetic variation assessment, and it can provide useful information for genetic improvement, preservation, and evaluation of wheat germplasm.","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135480295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-03DOI: 10.34133/plantphenomics.0115
Yanan Li, Yuling Tang, Yifei Liu, Dingrun Zheng
{"title":"Semi-supervised Counting of Grape Berries in the Field Based on Density Mutual Exclusion","authors":"Yanan Li, Yuling Tang, Yifei Liu, Dingrun Zheng","doi":"10.34133/plantphenomics.0115","DOIUrl":"https://doi.org/10.34133/plantphenomics.0115","url":null,"abstract":"Automated counting of grape berries has become one of the most important tasks in grape yield prediction. However, dense distribution of berries and the severe occlusion between berries bring great challenges to counting algorithm based on deep learning. The collection of data required for model training is also a tedious and expensive work. To address these issues and cost-effectively count grape berries, a semi-supervised counting of grape berries in the field based on density mutual exclusion (CDMENet) is proposed. The algorithm uses VGG16 as the backbone to extract image features. Auxiliary tasks based on density mutual exclusion are introduced. The tasks exploit the spatial distribution pattern of grape berries in density levels to make full use of unlabeled data. In addition, a density difference loss is designed. The feature representation is enhanced by amplifying the difference of features between different density levels. The experimental results on the field grape berry dataset show that CDMENet achieves less counting errors. Compared with the state of the arts, coefficient of determination (R2) is improved by 6.10%, and mean absolute error and root mean square error are reduced by 49.36% and 54.08%, respectively. The code is available at https://github.com/youth-tang/CDMENet-main.","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135821332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-03DOI: 10.1007/s43657-023-00128-8
Lizhen Lan, Kai Feng, Yudan Wu, Wenbo Zhang, Ling Wei, Huiting Che, Le Xue, Yidan Gao, Ji Tao, Shufang Qian, Wenzhao Cao, Jun Zhang, Chengyan Wang, Mei Tian
{"title":"Phenomic Imaging","authors":"Lizhen Lan, Kai Feng, Yudan Wu, Wenbo Zhang, Ling Wei, Huiting Che, Le Xue, Yidan Gao, Ji Tao, Shufang Qian, Wenzhao Cao, Jun Zhang, Chengyan Wang, Mei Tian","doi":"10.1007/s43657-023-00128-8","DOIUrl":"https://doi.org/10.1007/s43657-023-00128-8","url":null,"abstract":"Abstract Human phenomics is defined as the comprehensive collection of observable phenotypes and characteristics influenced by a complex interplay among factors at multiple scales. These factors include genes, epigenetics at the microscopic level, organs, microbiome at the mesoscopic level, and diet and environmental exposures at the macroscopic level. “Phenomic imaging” utilizes various imaging techniques to visualize and measure anatomical structures, biological functions, metabolic processes, and biochemical activities across different scales, both in vivo and ex vivo. Unlike conventional medical imaging focused on disease diagnosis, phenomic imaging captures both normal and abnormal traits, facilitating detailed correlations between macro- and micro-phenotypes. This approach plays a crucial role in deciphering phenomes. This review provides an overview of different phenomic imaging modalities and their applications in human phenomics. Additionally, it explores the associations between phenomic imaging and other omics disciplines, including genomics, transcriptomics, proteomics, immunomics, and metabolomics. By integrating phenomic imaging with other omics data, such as genomics and metabolomics, a comprehensive understanding of biological systems can be achieved. This integration paves the way for the development of new therapeutic approaches and diagnostic tools.","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135819535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant PhenomicsPub Date : 2023-11-03DOI: 10.34133/plantphenomics.0116
Eric Duchêne, Elsa Chedid, Komlan Avia, Vincent Dumas, Lionel Ley, Nicolas Reibel, Gisèle Butterlin, Maxime Soma, Raul Lopez-Lozano, Frédéric Baret, Didier Merdinoglu
{"title":"LiDAR is effective in characterizing vine growth and detecting associated genetic loci","authors":"Eric Duchêne, Elsa Chedid, Komlan Avia, Vincent Dumas, Lionel Ley, Nicolas Reibel, Gisèle Butterlin, Maxime Soma, Raul Lopez-Lozano, Frédéric Baret, Didier Merdinoglu","doi":"10.34133/plantphenomics.0116","DOIUrl":"https://doi.org/10.34133/plantphenomics.0116","url":null,"abstract":"The strong societal demand to reduce pesticide use and adaptation to climate change challenges the capacities of phenotyping new varieties in the vineyard. High-throughput phenotyping is a way to obtain meaningful and reliable information on hundreds of genotypes in a limited period. We evaluated traits related to growth in 209 genotypes from an interspecific grapevine biparental cross, between IJ119, a local genitor, and Divona, both in summer and in winter, using several methods: fresh pruning wood weight, exposed leaf area calculated from digital images, leaf chlorophyll concentration, and LiDAR-derived apparent volumes. Using high-density genetic information obtained by the genotyping by sequencing technology (GBS), we detected 6 regions of the grapevine genome [quantitative trait loci (QTL)] associated with the variations of the traits in the progeny. The detection of statistically significant QTLs, as well as correlations (R2) with traditional methods above 0.46, shows that LiDAR technology is effective in characterizing the growth features of the grapevine. Heritabilities calculated with LiDAR-derived total canopy and pruning wood volumes were high, above 0.66, and stable between growing seasons. These variables provided genetic models explaining up to 47% of the phenotypic variance, which were better than models obtained with the exposed leaf area estimated from images and the destructive pruning weight measurements. Our results highlight the relevance of LiDAR-derived traits for characterizing genetically induced differences in grapevine growth and open new perspectives for high-throughput phenotyping of grapevines in the vineyard.","PeriodicalId":20318,"journal":{"name":"Plant Phenomics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135821333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}