Journal of Tissue Engineering and Regenerative Medicine最新文献

{"title":"Recent Advancements in the Generation and Application of Therapeutic Cell Populations for Lung Epithelial Repair","authors":"Muyang Zhou,&nbsp;Dana Brinson,&nbsp;Cindy Lei,&nbsp;Golnaz Karoubi","doi":"10.1155/term/8367426","DOIUrl":"10.1155/term/8367426","url":null,"abstract":"<p>Chronic respiratory diseases are a major global health concern. Lung epithelial dysfunction is a common underlying feature of many such conditions; hence, reconstructing the diseased epithelium with functional epithelial cells is a promising therapeutic approach. There are various endogenous stem cell and progenitor populations in the lung epithelium that can be utilized for transplantation. Additionally, pluripotent stem cells (PSCs) have emerged as a valuable source for generating therapeutic cells due to their capacity for indefinite self-renewal and the availability of directed differentiation protocols to transform them into lung progenitors or mature lung epithelial cells. This review discusses the endogenous stem cell and progenitor populations of the lung epithelium, recent advances in developing directed differentiation protocols to generate these cells, and the application of both endogenous and PSC-derived lung epithelial cells for disease modeling in vitro and as cell therapies in vivo. It provides valuable insights into the current progress of regenerative medicine within the respiratory field and highlights areas that require further research.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12754274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145888352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keratin Additive for Cellular Adhesion in Transcutaneous Prosthetics","authors":"A. L. Cagle,&nbsp;E. L. Szulc,&nbsp;J. Flaggert,&nbsp;Y. Arias,&nbsp;A. Nikhar,&nbsp;D. Tadio,&nbsp;J. A. Durant,&nbsp;I. L. Gitajn,&nbsp;K. R. Hixon","doi":"10.1155/term/4337554","DOIUrl":"10.1155/term/4337554","url":null,"abstract":"<p>The dermal barrier is widely considered the body’s first line of defense against most foreign bodies, protecting it from both moisture loss and bacterial invasion. However, when the skin is ruptured for long-term medical interventions (e.g., transcutaneous prosthetics), it is difficult to restore and maintain this protective barrier. Although there are no direct, biological examples of true transcutaneous features in the human body, similar phenomena can be observed in phalangeal nails. This study aims to investigate keratin, the primary component of fingernails, in its hydrolyzed form as an additive to induce cell adhesion in two representative scaffold types. Electrospun fibers and chitosan–gelatin cryogels—two well-characterized scaffolds used in dermal tissue engineering—were selected for this study as a fibrous and macroporous foundation. Both electrospun fibers and cryogels were fabricated with a range of keratin additive concentrations (0, 1, 3, 5, 7, and 10 wt/wt% and wt/v% for electrospun fibers and cryogels, respectively) and tested for surface properties, mechanical strength, biocompatibility, and material behavior. Overall, it was determined that hydrolyzed keratin had a positive effect on cell adhesion and proliferation but that high quantities of the keratin resulted in adverse effects on the scaffold properties. With dermal applications in mind, this study found that 5 and 7 wt/wt% keratin electrospun fibers possessed required cell counts, surface energies, tensile strength, and contact angle, all with consistent reproducibility. For the cryogels, 3 and 5 wt/v% keratin had the best combined performance, maintained structural integrity through swelling and porosity, and displayed minimal loss in compressive strength. Therefore, hydrolyzed keratin represents a promising additive for bothelectrospun fibers and cryogels in tissue engineering applications.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12750100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amniotic Membrane and Mesenchymal Stem Cell Coalescence for Islet Transplantation in Experimental Diabetes in Rats","authors":"Meral Tiryaki,&nbsp;Nurgul Atmaca,&nbsp;Ferda Pinarli,&nbsp;Gulbahar Boyuk Ozcan,&nbsp;Mehmet Sedat Feyat,&nbsp;Sercan Mercan,&nbsp;Aynur Albayrak,&nbsp;Hasan Tarik Atmaca","doi":"10.1155/term/2645595","DOIUrl":"10.1155/term/2645595","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>The aim of this study is to investigate the effects of islet cells and mesenchymal stem cells transferred together in the amniotic membrane (AM) in order to preserve the viability and functionality of islet cells on the success of islet transplantation in diabetes mellitus–induced rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 80 male Wistar albino rats, aged 3.5–4 months, were included in this study. While 40 Wistar Albino rats were used for the process of islet cell isolation, 40 Wistar Albino rats were used to establish experimental groups. These rats were assigned to five experimental groups including eight rats in each. These groups were AM, amniotic membrane + mesenchymal stem cell (AM + MSC), amniotic membrane + islet cell (AM + IC), amniotic membrane + islet cell + mesenchymal stem cell (AM + IC + MSC), and sham groups. The study was concluded for 28 days.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Although there was no significant difference between AM + IC and AM + IC + MSC groups in terms of mean blood glucose levels, both groups had statistically different values compared to the sham group. A significant difference was observed between the AM + IC and AM + IC + MSC groups in the c-peptide levels before and after transplantation. Immunohistochemical staining illustrated the presence of insulin-positive cells in both AM + IC and AM + IC + MSC groups. Moreover, BrDU (+) cells were determined in AM + IC and AM + IC + MSC groups using BrDU staining.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The study results indicated that transplanting islet cells into the omentum by being packaged in AM preserved their viability and function, leading to significant effects on blood glucose and c-peptide levels.</p>\u0000 </section>\u0000 </div>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12721394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145814802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SphK1 Suppresses Human Dental Pulp Stem Cell Apoptosis by Promoting Glycolysis Under Simulated Microgravity","authors":"Jingyi Che,&nbsp;Zhengjun Qiu,&nbsp;Huailong Hou,&nbsp;Yanping Li,&nbsp;Lina He,&nbsp;Jingxuan Sun,&nbsp;Shuang Zhang,&nbsp;Mengdi Li,&nbsp;Shuang Pan,&nbsp;Weiwei Zhang,&nbsp;Yumei Niu","doi":"10.1155/term/6659059","DOIUrl":"10.1155/term/6659059","url":null,"abstract":"<p>Glycolysis supports mesenchymal stem cell (MSC) proliferation and sustains their undifferentiated state by maintaining energy supply and limiting apoptosis. The rapid advancement of space life sciences has spurred considerable interest in the effects of microgravity on stem cells. However, the contribution of glycolytic metabolism to apoptotic regulation under simulated microgravity (SMG) remains unclear. This study examined the influence of SMG on glycolytic activity and apoptosis in human dental pulp stem cells (hDPSCs). Lactic acid and glucose measurements were used to evaluate glycolytic flux, while transcript levels of HK2, PKM2, and LDHA were quantified by qPCR, HK2 and PKM2 protein expression was assessed by Western blotting, and annexin V-FITC/PI staining combined with immunoblotting of apoptosis-related proteins (BAX, BCL-2, and cleaved caspase-3) was performed to assess cell death. SMG markedly increased glycolytic capacity and attenuated apoptosis in hDPSCs. SphK1 expression was also elevated, indicating a role in cell survival. Pharmacological inhibition of SphK1 with PF-543 reduced both glycolysis and the antiapoptotic effect, implicating SphK1 as a critical regulator of these processes. Inhibition of glycolysis by 2-DG further increased apoptosis, confirming the protective role of glycolytic metabolism under SMG. These findings demonstrate that SMG enhances glycolysis and limits apoptosis in hDPSCs via SphK1 upregulation, suggesting that microgravity conditions may augment stem cell survival and function.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12714168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145802782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Chorioallantoic Membrane as a Platform for Developing Vascularized Cell Macroencapsulation Devices","authors":"Murillo D. L. Bernardi,&nbsp;Sonny F. de Jong,&nbsp;Maarten B. Rookmaker,&nbsp;Andrej Shoykhet,&nbsp;Roel Deckers,&nbsp;Silvia M. Mihăilă,&nbsp;Rosalinde Masereeuw,&nbsp;Marianne C. Verhaar","doi":"10.1155/term/5577199","DOIUrl":"https://doi.org/10.1155/term/5577199","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Cell macroencapsulation devices (CMD) offer a promising solution for organ function replacement by shielding implanted cells from the host immune system while allowing the exchange of nutrients and waste products. Developing efficient CMD necessitates optimizing vascular integration, membrane permeability, and cellular functionality using robust preclinical models. In this study, we adapted the chick chorioallantoic membrane (CAM) model to develop and evaluate CMD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Semipermeable membranes were integrated into the CAM, with vascularization modulated through growth factors and extracellular matrix manipulation. Human kidney tubular epithelial cells were cultured on these vascularized membranes to assess cell viability, polarization, and functionality, including selective transport and barrier integrity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The membranes integrated successfully into the CAM and supported functional vascularization, demonstrating selective permeability by facilitating the exchange of low-molecular-weight compounds while preventing the infiltration of larger proteins and cells, thereby creating an immune-isolated environment. Kidney tubular epithelial cells remained viable, polarized, and functionally active, showcasing selective compound transport and robust barrier integrity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings underscore the CAM model’s utility in evaluating vascular integration, membrane permeability, and epithelial cell functionality, all critical parameters for CMD development. The CAM model provides a rapid, cost-effective platform for CMD assessment, significantly accelerating their development and potential clinical translation. This approach holds particular promise for applications targeting kidney diseases characterized by compromised transport functions, offering a pathway toward more effective therapeutic solutions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/term/5577199","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Retinal Progenitor Cell (hRPC) Migration in Three-Dimensional (3D) Environments of Varying Stiffness and Composition","authors":"Peng Zhao,&nbsp;Joydip Kundu,&nbsp;Douglas Blanton,&nbsp;Mahboobeh Rezaeeyazdi,&nbsp;Madeleine J. Oudin,&nbsp;Miles A. Miller,&nbsp;Aaron S. Meyer,&nbsp;Sidi A. Bencherif,&nbsp;Petr Y. Baranov,&nbsp;Michael J. Young,&nbsp;Rebecca L. Carrier","doi":"10.1155/term/9963972","DOIUrl":"https://doi.org/10.1155/term/9963972","url":null,"abstract":"<p>Retinal degeneration is the leading cause of blindness worldwide. Subretinal implantation of human retinal progenitor cells (hRPCs) has shown great promise in models of retinal degeneration for restoration of vision but is limited by extremely low (&lt; 2%) integration into the retina. Successful integration of implanted cells requires their migration from the site of implantation into the degenerating retina. Little is known about what cues promote RPC migration in the context of the postimplantation microenvironment, such as cues presented by a biomaterial carrier. We utilized a high-throughput assay to study the migration of hRPCs in three-dimensional hydrogel matrices of varying chemical composition and stiffness and, with exposure to different soluble factors, to identify cues important for hRPC migration and associated cell signaling events driving migration. Collagen type I, collagen type I methacrylate, and hyaluronic acid glycidyl methacrylate gels were developed with variable stiffness. The impact of key growth factors in neural development, regeneration, and cell migration such as epidermal growth factor (EGF), fibroblast growth factor (FGF), stromal cell–derived factor (SDF), and hepatocyte growth factor (HGF) was studied using hRPCs in 2 mg/mL collagen type I gels. Migration of the hRPCs varied significantly in gels of different composition and stiffness, with higher levels of mean migration distance after 48 h in nonphoto crosslinked collagen-based gels with higher concentrations of gel components and associated compressive moduli. In addition, the presence of SDF and HGF in collagen gels increased hRPC migration compared to media alone. Key signaling nodes correlating with hRPC migration were identified in Akt and MAPK signaltransduction pathways using bead-based multiplex ELISA and partial least-squares regression (PLSR) modeling. These results motivate the further exploration of material stiffness and co-delivery of soluble factors as important design parameters in cell delivery vehicles to promote transplanted hRPC migration and successful integration into degenerating retina.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/term/9963972","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145406904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ex Vivo Organ Perfusion Systems for Disease Modeling and Therapeutic Applications in Small Animal Models","authors":"Mina Yeganeh,&nbsp;Andea Zito,&nbsp;Marwa Sadat,&nbsp;Agostino Pierro,&nbsp;Ian M. Rogers","doi":"10.1155/term/2583925","DOIUrl":"https://doi.org/10.1155/term/2583925","url":null,"abstract":"<p>Ex vivo organ perfusion (EVOP) is used for whole organ preservation, and the main focus is to improve the outcome of donor organs for transplantation. Recently, EVOP has found application in disease modeling, drug development, and tissue regeneration. We discuss progress in EVOP research involving small animal organs using benchtop and incubator-based EVOP systems, highlighting innovative designs of EVOP systems, technical specifications of each system, and their versatile applications across a range of research fields.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/term/2583925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145101782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Design and development of thyroxine/heparin releasing affordable cotton dressings to treat chronic wounds","authors":"Journal of Tissue Engineering and Regenerative Medicine","doi":"10.1155/term/9795348","DOIUrl":"https://doi.org/10.1155/term/9795348","url":null,"abstract":"<p>RETRACTION: T. S. Waris, S. T. Abbas Shah, A. Mehmood, et al., “Design and development of thyroxine/heparin releasing affordable cotton dressings to treat chronic wounds,” <i>Journal of Tissue Engineering and Regenerative Medicine</i> 2022 (2022): term.3295, https://doi.org/10.1002/term.3295.</p><p>The above article, published online on 4 March 2022 in Wiley Online Library (https://wileyonlinelibrary.com), has been retracted by agreement between the journal’s Chief Editor Catherine K. Kuo and John Wiley &amp; Sons Ltd.</p><p>The retraction has been agreed following an investigation of the concerns raised by <i>Mycosphaerella arachidis</i> on PubPeer [<span>1</span>], which identified unexpected similarities between multiple panels of Figure 6(a).</p><p>More specifically, the images depicting the wounds of animals in the Hep-W and Control-W groups after 20 days appear identical to the image of the T4-W group after 15 days. Further concerns have also been identified related to the similarity of two panels to images shown in Figure 7 from another publication by the author group, where they are attributed to a different experimental condition [<span>2</span>].</p><p>As a result of the investigation, the data and conclusions of this article are considered unreliable.</p><p>The authors disagree with this retraction.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/term/9795348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: “Mechanical stretch promotes antioxidant responses and cardiomyogenic differentiation in P19 cells”","authors":"Journal of Tissue Engineering and Regenerative Medicine","doi":"10.1155/term/9851972","DOIUrl":"https://doi.org/10.1155/term/9851972","url":null,"abstract":"<p>RETRACTION: J. Cheng, Q. Zou, Y. Xue, C. Sun, and D. Zhang, “Mechanical stretch promotes antioxidant responses and cardiomyogenic differentiation in P19 cells,” <i>Journal of Tissue Engineering and Regenerative Medicine</i>, 2021 (2021): term.3184, https://doi.org/10.1002/term.3184.</p><p>The above article, published online on 20 March 2021 in Wiley Online Library (https://wileyonlinelibrary.com), has been retracted following the agreement of the journal’s Chief Editor Catherine Kuo; and John Wiley &amp; Sons Ltd.</p><p>The retraction has been agreed following an investigation of the concerns raised by Hoya Camphorifolia on PubPeer [<span>1</span>], which identified concerns regarding Figures 4 and 5.</p><p>Specifically, similarity has been identified between the Western blot bands corresponding to SIRT 1 and GAPDH in Figure 4(b) to c-Casp 3 and c-Casp 9 bands in Figure 3(d) of [<span>2</span>]. Unexpected similarities were also identified between the cTNT and Cx43 bands in Figures 5(b), 5(d) and the Bcl-2 and Bad blots in Figure 3(d) of [<span>2</span>].</p><p>As a result of the investigation, the data and conclusions of this article are considered unreliable.</p><p>The authors were informed of the decision to retract the article but did not provide a response.</p>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/term/9851972","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viewing Decellularized Amniotic Membrane Through the Lens of Coupled Scaffolding and Drug Delivery Systems in Regenerative Medicine","authors":"Fatemeh Alibabaei-Omran,&nbsp;Nima Javanmehr,&nbsp;Atiyeh Al-e-Ahmad,&nbsp;Ebrahim Zabihi,&nbsp;Tohid Najafi","doi":"10.1155/term/8818058","DOIUrl":"https://doi.org/10.1155/term/8818058","url":null,"abstract":"<div>\u0000 <p>Regenerative medicine (RM) exploits stem cells to construct biological replacements and repair damaged tissues, offering an alternative to daunting organ transplantation. Even while RM has advanced quickly, building an entire organ remains beyond our capabilities. Experts are thus investigating the adoption of biologically generated composites that preserve the tissue’s crucial physiological, morphological, and mechanical characteristics. Noncellular tissues like extracellular matrix offer cells a milieu similar to their physiological niche, becoming a promising substitute for synthetic composites. In this context, amnion, the membrane enclosing the fetus, is a great contender since it is widely obtainable and economical. Given its biochemical and anatomic characteristics, and the extensive supply of stem cells, growth factors, and matrix proteins, the amnion is considered a fantastic candidate to employ in RM. Decellularized amniotic membrane (DAM) has many uses as two- and three-dimensional scaffolds, anchoring for cell adhesion and expansion for tissue regeneration, and as carrier systems for cell and drug cargoes. The present research aims to assess the recent surge in DAM-RM research, potentially to get beyond the existing barriers impeding the RM’s clinical translation landscape. The present paper draws a comprehensive picture of the experimental evidence and clinical trials regarding exploiting DAM in RM.</p>\u0000 </div>","PeriodicalId":202,"journal":{"name":"Journal of Tissue Engineering and Regenerative Medicine","volume":"2025 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/term/8818058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}