Polish Journal of Microbiology最新文献_第3页

Prevalence of Closely Related Candida albicans Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the hwp1 Gene Amplification. 基于hwp1基因扩增的波兰南部外阴阴道念珠菌病患者中密切相关白色念珠菌的流行

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-03-01 DOI: 10.33073/pjm-2023-011

Karolina Klesiewicz, Paulina Mrowiec, Katarzyna Kania, Iwona Skiba-Kurek, Joanna Białecka, Magdalena Namysł, Marianna Małek

{"title":"Prevalence of Closely Related Candida albicans Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the hwp1 Gene Amplification.","authors":"Karolina Klesiewicz, Paulina Mrowiec, Katarzyna Kania, Iwona Skiba-Kurek, Joanna Białecka, Magdalena Namysł, Marianna Małek","doi":"10.33073/pjm-2023-011","DOIUrl":"https://doi.org/10.33073/pjm-2023-011","url":null,"abstract":"Candida albicans remains the most common species isolated from women with vulvovaginal candidiasis. However, closely related species such as Candida africana and Candida dubliniensis may also occur, although they are often misidentified. The aim of the study was to confirm the phenotypic identification of C. albicans and its closely related species isolated from women with genital tract infections by amplification of the hwp1 (hyphal wall protein 1) gene in a PCR assay. We report a detailed molecular identification of C. albicans and its closely related species among 326 patients in the Małopolska region, Poland. Initial phenotypic identifications were confirmed by amplification of the hwp1 gene. Based on molecular analysis, we revealed 307 strains (94.17%) as C. albicans and 17 as C. dubliniensis (5.22%). No strain of C. africana was detected. Two patients h ad co-infection with C. albicans and C. dubliniensis (0.61%). A PCR assay targeting the hwp1 gene was reliable for correctly identifying species among the C. albicans complex.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/92/1e/pjm-72-1-pjm-2023-011.PMC10280305.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10062276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China. 浙江省常见水产品中细菌耐药性的初步研究

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-03-01 DOI: 10.33073/pjm-2023-010

Jiajia Wu, Fan Ye, Jiong Qu, Zhiyuan Dai

{"title":"Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China.","authors":"Jiajia Wu, Fan Ye, Jiong Qu, Zhiyuan Dai","doi":"10.33073/pjm-2023-010","DOIUrl":"https://doi.org/10.33073/pjm-2023-010","url":null,"abstract":"The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. Aeromonas spp., Shewanella spp., Acinetobacter spp., Myroides spp., Pseudomonas spp., and Citrobacter spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes qnrS, tetA, floR, and cmlA were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes sul2, aac(6')-Ib, and bla PSE were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/d4/pjm-72-1-pjm-2023-010.PMC10280302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9705083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Plant Growth-Promoting Endohyphal Bacillus subtilis in Fusarium acuminatum from Spiranthes sinensis. 螺旋藻尖锐镰刀菌促生长内生枯草芽孢杆菌的鉴定。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-03-01 DOI: 10.33073/pjm-2023-007

Lan Fang, Xiao Zheng, Zhengxiang Sun, Yanyan Li, Jianxin Deng, Y I Zhou

{"title":"Characterization of a Plant Growth-Promoting Endohyphal Bacillus subtilis in Fusarium acuminatum from Spiranthes sinensis.","authors":"Lan Fang, Xiao Zheng, Zhengxiang Sun, Yanyan Li, Jianxin Deng, Y I Zhou","doi":"10.33073/pjm-2023-007","DOIUrl":"https://doi.org/10.33073/pjm-2023-007","url":null,"abstract":"Successful seed germination and seedling growth in orchids require an association with mycorrhizal fungi. An endophytic Fusarium fungal strain YZU 172038 exhibiting plant growth-promoting (PGP) ability was isolated from the roots of Spiranthes sinensis (Orchidaceae). The harboring endohyphal bacteria were detected in the hypha by SYTO-9 fluorescent nucleic acid staining, fluorescence in situ hybridization (FISH), and PCR amplification of the 16S rDNA gene's region. Consequently, one endohyphal bacterium (EHB) - a strain YZSR384 was isolated and identified as Bacillus subtilis based on morphology, phylogenetic analysis, and genomic information. The results indicated that the strain YZSR384 could significantly promote the growth of rice roots and shoots similar to its host fungus. Its indole acetic acid (IAA) production reached a maximum of 23.361 μg/ml on the sixth day after inoculation. The genome annotation revealed several genes involved in PGP traits, including the clusters of genes encoding the IAA (trpABCDEFS), the siderophores (entABCE), and the dissolving phosphate (pstABCS and phoABDHPR). As an EHB, B. subtilis was first isolated from endophytic Fusarium acuminatum from S. sinensis.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/04/pjm-72-1-pjm-2023-007.PMC10280306.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9705077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics and Prebiotics as Dietary Supplements for the Adjunctive Treatment of Type 2 Diabetes. 益生菌和益生元作为辅助治疗2型糖尿病的膳食补充剂。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2023-03-01 DOI: 10.33073/pjm-2023-013

Yuying Wang, Lina Wen, Huazhen Tang, Jinxiu Qu, Benqiang Rao

引用次数: 1

The Emergence of Colistin- and Imipenem-Associated Multidrug Resistance in Escherichia coli Isolates from Retail Meat. 从零售肉类中检出的大肠埃希菌出现了与考利司汀和亚胺培南相关的多重耐药性。

IF 2 4区生物学

Polish Journal of Microbiology Pub Date : 2022-12-06 eCollection Date: 2022-12-01 DOI: 10.33073/pjm-2022-046

Benson Chuks Iweriebor, Olivia Sochi Egbule, Larry C Obi

{"title":"The Emergence of Colistin- and Imipenem-Associated Multidrug Resistance in Escherichia coli Isolates from Retail Meat.","authors":"Benson Chuks Iweriebor, Olivia Sochi Egbule, Larry C Obi","doi":"10.33073/pjm-2022-046","DOIUrl":"10.33073/pjm-2022-046","url":null,"abstract":"To determine the prevalence of Escherichia coli and their drug resistance profiles in fresh pork sold at two retail outlets (open-air market and closed retail stores) in Alice, South Africa. Retail meat samples (n = 176) collected from four shops (two from open-air markets and two from closed stores) were analyzed by conventional biochemical and PCR-based molecular confirmatory tests. The confirmed isolates were profiled for antimicrobial susceptibility to a panel of 12 commercial antibiotics: tetracycline, ampicillin, sulphamethoxazole trimethoprim, erythromycin, gentamycin, colistin sulphate, cefotaxime, chloramphenicol, norfloxacin, ciprofloxacin, cefuroxime, and imipenem. Colistin, ampicillin, and erythromycin resistance genes were profiled with the gene-specific primers. Multidrug resistance (MDR) and the association of imipenem and colistin in the MDR profile were determined. A total of 68 (39.08%) E. coli isolates were confirmed by PCR analysis. Resistance was most common to erythromycin (100%), followed by cefotaxime (95.58%), ampicillin (88.23%), cefuroxime (88.23%), trimethoprim-sulphamethoxazole (88.23%), and tetracycline (60.29%). Overall, 27/68 (39.70%) were MDR (≥ 3antibiotics classes). MDR E. coli isolates associated with imipenem resistance (50.00%) and colistin resistance (33.82%) were detected. The resistance genes were detected among the isolates though not in all the phenotypically resistant isolates. The detection of colistin resistance among MDR E. coli isolates from retail meat is troubling as the drug is a last resort antibiotic. Overall, the epidemiological implications of the findings are of public health importance.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/7a/pjm-71-519.PMC9944967.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9309829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Omicron SARS-CoV-2 Variants in an In Silico Genomic Comparison Study with the Original Wuhan Strain and WHO-Recognized Variants of Concern. SARS-CoV-2基因组变异与原武汉株和世卫组织认可的关注变异的计算机基因组比较研究

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2022-12-01 DOI: 10.33073/pjm-2022-053

Elmutuz H Elssaig, Tarig M S Alnour, Mohammad Fahad Ullah, Eltayib H Ahmed-Abakur

{"title":"Omicron SARS-CoV-2 Variants in an In Silico Genomic Comparison Study with the Original Wuhan Strain and WHO-Recognized Variants of Concern.","authors":"Elmutuz H Elssaig, Tarig M S Alnour, Mohammad Fahad Ullah, Eltayib H Ahmed-Abakur","doi":"10.33073/pjm-2022-053","DOIUrl":"https://doi.org/10.33073/pjm-2022-053","url":null,"abstract":"This study aimed to determine the genetic alterations in the Omicron variants compared to other variants of concern (VOCs) to trace the evolutionary genetics of the SARS-CoV-2 variants responsible for the multiple COVID-19 waves globally. The present study is an in silico analysis determining the evolution of selected 11 VOCs compared to the original Wuhan strain. The variants included six Omicrons and one variant of Alpha, Beta, Delta, Gamma, and Mu. The pairwise alignment with the local alignment search tool of NCBI Nucleotide-BLAST and NCBI Protein-BLAST were used to determine the nucleotide base changes and corresponding amino acid changes in proteins, respectively. The genomic analysis revealed 210 nucleotide changes; most of these changes (127/210, 60.5%) were non-synonymous mutations that occurred mainly in the S gene (52/127, 40.1%). The remaining 10.5% (22/210) and 1.9% (4/210) of the mutations were frameshift deletions and frameshift insertions, respectively. The frameshift insertion (Ins22194T T22195G) led to frameshift deletion (Δ211N). Only four mutations (C241T, C3037T, C14408T, and A23403G) were shared among all the VOCs. The nucleotide changes among Omicron variants resulted in 61 amino acid changes, while the nucleotide changes in other VOCs showed 11 amino acid changes. The present study showed that most mutations (38/61, 62.3%) among Omicron variants occurred in the S gene; and 34.2% of them (13/38) occurred in the receptor-binding domain. The present study confirmed that most of mutations developed by Omicron variants occurred in the vaccine target gene (S gene).","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/28/pjm-71-577.PMC9944972.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9310346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of Fecal Microbiomes of Osteoporotic Patients in Korea. 韩国骨质疏松症患者粪便微生物组特征分析。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2022-12-01 DOI: 10.33073/pjm-2022-045

Asad Ul-Haq, Hoonhee Seo, Sujin Jo, Hyuna Park, Sukyung Kim, Youngkyoung Lee, Saebim Lee, Je Hoon Jeong, Ho-Yeon Song

{"title":"Characterization of Fecal Microbiomes of Osteoporotic Patients in Korea.","authors":"Asad Ul-Haq, Hoonhee Seo, Sujin Jo, Hyuna Park, Sukyung Kim, Youngkyoung Lee, Saebim Lee, Je Hoon Jeong, Ho-Yeon Song","doi":"10.33073/pjm-2022-045","DOIUrl":"https://doi.org/10.33073/pjm-2022-045","url":null,"abstract":"An imbalanced gut microbiome has been linked to a higher risk of many bone-related diseases. The objective of this study was to discover biomarkers of osteoporosis (OP). So, we collected 76 stool samples (60 human controls and 16 OP patients), extracted DNA, and performed 16S ribosomal ribonucleic acid (rRNA) gene-based amplicon sequencing. Among the taxa with an average taxonomic composition greater than 1%, only the Lachnospira genus showed a significant difference between the two groups. The Linear Discriminant Effect Size analysis and qPCR experiments indicated the Lachnospira genus as a potential biomarker of OP. Moreover, a total of 11 metabolic pathways varied between the two groups. Our study concludes that the genus Lachnospira is potentially crucial for diagnosing and treating osteoporosis. The findings of this study might help researchers better understand OP from a microbiome perspective. This research might develop more effective diagnostic and treatment methods for OP in the future.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c3/b5/pjm-71-601.PMC9944973.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9309863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ciliated Peritrichous Protozoa in a Tezontle-Packed Sequencing Batch Reactor as Potential Indicators of Water Quality. tezontl -填料序批式反应器中纤毛含氮原生动物作为水质潜在指标。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2022-12-01 DOI: 10.33073/pjm-2022-049

Andrea M Rivas-Castillo, Antonio A Garcia-Barrera, Aristeo Garrido-Hernandez, Francisco J Martinez-Valdez, Maria S Cruz-Romero, Maribel Quezada-Cruz

{"title":"Ciliated Peritrichous Protozoa in a Tezontle-Packed Sequencing Batch Reactor as Potential Indicators of Water Quality.","authors":"Andrea M Rivas-Castillo, Antonio A Garcia-Barrera, Aristeo Garrido-Hernandez, Francisco J Martinez-Valdez, Maria S Cruz-Romero, Maribel Quezada-Cruz","doi":"10.33073/pjm-2022-049","DOIUrl":"https://doi.org/10.33073/pjm-2022-049","url":null,"abstract":"The presence of colonial and solitary ciliated peritrichous protozoa was determined in a Sequencing Batch Reactor system filled with tezontle, a volcanic rock, economic, and abundant material that can be found in some parts of the world, like Mexico. The presence of these protozoa was related to the removal efficiencies of organic matter. Also, two novel staining techniques are proposed for staining both colonial and solitary peritrichous protozoa. The results show that tezontle promotes the growth of solitary and colonial ciliated peritrichous protozoa, which, once identified, could be used as indicators of the efficiency of the wastewater treatment process. Additionally, the staining techniques established in the current study allowed the precise observation of protozoan nuclei. They can represent a useful complementary methodology for identifying protozoan species present in water treatment processes, along with the already existing identification techniques. The number and variety of protozoa found in the system may be considered potential bioindicators of water quality during biological treatments.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d6/28/pjm-71-539.PMC9944969.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10762236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Cell-Free DNA-Based Next-Generation Sequencing for Identifying Pathogens in Bacteremia Patients. 基于无细胞dna的新一代测序鉴定菌血症患者病原体的评估。

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2022-12-01 DOI: 10.33073/pjm-2022-043

Chaoqin Zhang, Hang Cheng, Yuxi Zhao, Jinlian Chen, Meng Li, Zhijian Yu, Xiang Sun, Peiyu Li, Yongpeng Shang, Jinmin Ma, Jinxin Zheng

{"title":"Evaluation of Cell-Free DNA-Based Next-Generation Sequencing for Identifying Pathogens in Bacteremia Patients.","authors":"Chaoqin Zhang, Hang Cheng, Yuxi Zhao, Jinlian Chen, Meng Li, Zhijian Yu, Xiang Sun, Peiyu Li, Yongpeng Shang, Jinmin Ma, Jinxin Zheng","doi":"10.33073/pjm-2022-043","DOIUrl":"https://doi.org/10.33073/pjm-2022-043","url":null,"abstract":"Rapid detection of bloodstream pathogens would greatly facilitate clinicians to make precise antimicrobial treatment in patients with bacteremia. In this study, 114 plasma samples were collected from patients with identified or suspected bacteremia, and pathogens were detected by the conventional blood culture (BC) and cell-free DNA metagenomics next-generation sequencing (cfDNA mNGS). The present study indicated that 76% (38/50) of positive conventional blood culture (BC+ group) patients were positively detected by cfDNA mNGS, and only 4% were mismatched between cfDNA mNGS and conventional bacteria culture. Pathogens in 32.8% of suspected bacteremia patients with negative conventional blood culture (BC- group) were determined accurately by cfDNA mNGS combined with analyzing the patients' clinical manifestations. Escherichia coli and Klebsiella pneumoniae were the most detected pathogens in identified bacteremia patients by cfDNA mNGS. 76.2% (16/21) of E. coli and 92.3% (12/13) of K. pneumoniae in bacteremia patients were identified by conventional blood cultures that were also detected by cfDNA mNGS. This study demonstrated that genomic coverage of E. coli and K. pneumoniae were more often detected in BC+ group patients and genomic coverage of Acinetobacter johnsonii and Paucibacter sp. KCTC 42545 was more often detected in BC- group patients. In conclusion, cfDNA mNGS could rapidly and precisely provide an alternative detection method for the diagnosis of bacteremia.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e9/3e/pjm-71-499.PMC9944966.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10749970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Screening of Toxin Genes in Methicillin-Resistant Staphylococcus aureus Clinical Isolates from a Hospital Setting in a Tertiary Hospital in Northern Cyprus. 北塞浦路斯某三级医院耐甲氧西林金黄色葡萄球菌临床分离株毒素基因筛选

IF 2.1 4区生物学

Polish Journal of Microbiology Pub Date : 2022-12-01 DOI: 10.33073/pjm-2022-042

Tchamou M F Potindji, Osaid A A Momani, Bakare B Omowumi, Buket Baddal

{"title":"Screening of Toxin Genes in Methicillin-Resistant Staphylococcus aureus Clinical Isolates from a Hospital Setting in a Tertiary Hospital in Northern Cyprus.","authors":"Tchamou M F Potindji, Osaid A A Momani, Bakare B Omowumi, Buket Baddal","doi":"10.33073/pjm-2022-042","DOIUrl":"https://doi.org/10.33073/pjm-2022-042","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) is a significant opportunistic pathogen with a wide repertoire of virulence characteristics. Data regarding the molecular profile of MRSA in Northern Cyprus is limited. The current study aimed to examine the virulence profiles of MRSA with a focus on toxin-associated factors. Ninety-one S. aureus isolates collected at a university hospital were included in the study. Identification and antibiotic susceptibility testing were performed with BD Phoenix™ automated system. Methicillin resistance was evaluated by the disc diffusion assay and mecA detection. The presence of nuc was confirmed by conventional PCR. Confirmed MRSA isolates were assessed for the presence of virulence genes hla, eta, etb, etd and tst using molecular methods. Among 91 S. aureus isolates identified as MRSA using the BD Phoenix™ platform, 80.85% (n = 76/91) were confirmed as MRSA using phenotypic and genotypic methods. All confirmed MRSA isolates (n = 76, 100%) were positive for the nuc. MRSA rates were statistically higher in elderly inpatients. The prevalence of toxin-encoding genes was 97.3% (n = 74/76) for hla, 2.63% (n = 2/76) for eta, 1.3% (n = 1/76) for etb, and 2.63% (n = 2/76) for tst. None of the screened isolates harbored the etd gene. These results represent the first report to investigate multiple virulence factors in MRSA isolates in Northern Cyprus.","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/81/0b/pjm-71-491.PMC9944970.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10766141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2