Characterization of Fecal Microbiomes of Osteoporotic Patients in Korea.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-12-01 DOI:10.33073/pjm-2022-045

Asad Ul-Haq, Hoonhee Seo, Sujin Jo, Hyuna Park, Sukyung Kim, Youngkyoung Lee, Saebim Lee, Je Hoon Jeong, Ho-Yeon Song

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引用次数: 4

Abstract

An imbalanced gut microbiome has been linked to a higher risk of many bone-related diseases. The objective of this study was to discover biomarkers of osteoporosis (OP). So, we collected 76 stool samples (60 human controls and 16 OP patients), extracted DNA, and performed 16S ribosomal ribonucleic acid (rRNA) gene-based amplicon sequencing. Among the taxa with an average taxonomic composition greater than 1%, only the Lachnospira genus showed a significant difference between the two groups. The Linear Discriminant Effect Size analysis and qPCR experiments indicated the Lachnospira genus as a potential biomarker of OP. Moreover, a total of 11 metabolic pathways varied between the two groups. Our study concludes that the genus Lachnospira is potentially crucial for diagnosing and treating osteoporosis. The findings of this study might help researchers better understand OP from a microbiome perspective. This research might develop more effective diagnostic and treatment methods for OP in the future.

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韩国骨质疏松症患者粪便微生物组特征分析。

不平衡的肠道微生物群与许多骨骼相关疾病的高风险有关。本研究的目的是发现骨质疏松症(OP)的生物标志物。因此，我们收集了76份粪便样本(60例人类对照和16例OP患者)，提取DNA，并进行了16S核糖体核糖核酸(rRNA)基因扩增子测序。在平均分类组成大于1%的分类群中，只有毛螺旋体属在两组间存在显著差异。线性判别效应(Linear Discriminant Effect Size)分析和qPCR实验表明，毛螺旋体属是op的潜在生物标志物。此外，两组之间共有11条代谢途径存在差异。我们的研究得出结论，毛螺旋体属对骨质疏松症的诊断和治疗具有潜在的关键作用。这项研究的发现可能有助于研究人员从微生物组的角度更好地理解OP。本研究可能在未来为OP提供更有效的诊断和治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464