Platelets最新文献

{"title":"Platelet mass cytometry reveals dysregulation of prothrombotic pathways in essential thrombocythemia.","authors":"Veronika Dill, Kilian Kirmes, Jiaying Han, Melissa Klug, Marc Rosenbaum, Giacomo Viggiani, Moritz von Scheidt, Markus List, Peter Herhaus, Jürgen Ruland, Florian Bassermann, Karl-Ludwig Laugwitz, Katharina S Götze, Philipp J Jost, Stefanie Jilg, Conor J Bloxham, Philip W J Raake, Isabell Bernlochner, Dario Bongiovanni","doi":"10.1080/09537104.2024.2358244","DOIUrl":"https://doi.org/10.1080/09537104.2024.2358244","url":null,"abstract":"<p><p>Thromboembolic events are common in patients with essential thrombocythemia (ET). However, the pathophysiological mechanisms underlying the increased thrombotic risk remain to be determined. Here, we perform the first phenotypical characterization of platelet expression using single-cell mass cytometry in six ET patients and six age- and sex-matched healthy individuals. A large panel of 18 transmembrane regulators of platelet function and activation were analyzed, at baseline and after <i>ex-vivo</i> stimulation with thrombin receptor-activating peptide (TRAP). We detected a significant overexpression of the activation marker CD62P (p-Selectin) (<i>p</i> = .049) and the collagen receptor GPVI (<i>p</i> = .044) in non-stimulated ET platelets. In contrast, ET platelets had a lower expression of the integrin subunits of the fibrinogen receptor GPIIb/IIIa CD41 (<i>p</i> = .036) and CD61 (<i>p</i> = .044) and of the von Willebrand factor receptor CD42b (<i>p</i> = .044). Using the FlowSOM algorithm, we identified 2 subclusters of ET platelets with a prothrombotic expression profile, one of them (cluster 3) significantly overrepresented in ET (22.13% of the total platelets in ET, 2.94% in controls, <i>p</i> = .035). Platelet counts were significantly increased in ET compared to controls (<i>p</i> = .0123). In ET, MPV inversely correlated with platelet count (<i>r</i>=-0.96). These data highlight the prothrombotic phenotype of ET and postulate GPVI as a potential target to prevent thrombosis in these patients.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2358244"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of platelet thrombus formation in patients following severe thermal injury.","authors":"Ali Asiri, Joshua M J Price, Jon Hazeldine, Kirsty C McGee, Amanda V Sardeli, Yung-Yi Chen, Jack Sullivan, Naiem S Moiemen, Paul Harrison","doi":"10.1080/09537104.2024.2420952","DOIUrl":"10.1080/09537104.2024.2420952","url":null,"abstract":"<p><p>Severe thermal injury significantly impacts upon hemostasis and is associated with classical changes to the circulating platelet count with a nadir followed by a rebound thrombocytosis at days ~3 and ~15 post-injury, respectively. To date, few studies have assessed platelet function following thermal injury as platelet tests often require large quantities of blood, are not representative of normal platelet pathophysiology, and are usually dependent on a normal platelet count. The purpose of this study was to measure platelet thrombus formation <i>in vitro</i> using a whole blood flow chip-based system following thermal injury and to study how platelet counts may impact upon the measurement. Adult (≥16 years) patients (<i>N</i> = 10) with ≥ 20% total burn surface area (TBSA) burn were recruited within 24 h of injury. Healthy controls (<i>N</i> = 25) were also recruited. Whole blood counts were measured using a hematology analyzer (Sysmex XN-1000). Platelet function was measured using the Total Thrombus-formation Analyzer System (T-TAS) within chips coated with tissue factor and collagen at shear rates of either 600 sec^-1 (AR chips) or 1200 sec^-1 (HD chips), the latter test being independent of platelet count. We confirmed the classical nadir in platelet counts following severe thermal injury at days 2, 3, 4 (<i>p</i> < 0.0001) and day 5 (<i>p</i> < 0.01) post-injury compared to healthy controls. Physiological platelet thrombus formation was significantly (<i>p</i> < 0.01) abnormal at day 3 post-injury using the AR chips but was related to the platelet count. However, although platelet dysfunction was not significant using HD chips, some of the results were independent of platelet count. A small number of samples, however, still gave abnormal results suggesting that there can be an underlying acquired platelet functional abnormality. Furthermore, the AR chip Area Under the Curve (AUC) was significantly lower on day 1 post-injury and negatively associated with severity of injury (TBSA, <i>p</i> < 0.05) and higher platelet function (AUC) positively associated with survival (<i>p</i> < 0.05). This study suggests that measuring platelet dysfunction within a more physiological <i>in vitro</i> test may have potential clinical utility. Larger studies are required to fully understand the impact of platelet dysfunction following severe thermal injury.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2420952"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential utilization of platelet-derived extracellular vesicles in clinical treatment","authors":"Zhijia Fan, Yixiao Gan, Yanwei Hu","doi":"10.1080/09537104.2024.2397592","DOIUrl":"https://doi.org/10.1080/09537104.2024.2397592","url":null,"abstract":"Platelet-derived extracellular vesicles (PEVs) are released by platelets in the blood circulation, which carry a rich bio-molecular cargo influential in intercellular communications. PEVs can enter...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"10 1","pages":"2397592"},"PeriodicalIF":3.3,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The safety and efficacy of indobufen or aspirin combined with clopidogrel in patients with acute myocardial infarction after percutaneous coronary intervention","authors":"Wen-Bo Dai, Jia-Yi Ren, Su-Tao Hu, Yu-Kun Zhang, Tian-Shu Gu, Xue Wu, Jing-Kun Zhang, Jing-Jin Che, Xiang-Hong Ma, Tong Liu, Guang-Ping Li, Kang-Yin Chen","doi":"10.1080/09537104.2024.2364748","DOIUrl":"https://doi.org/10.1080/09537104.2024.2364748","url":null,"abstract":"Currently, the standard treatment for patients who have undergone percutaneous coronary intervention (PCI) following acute myocardial infarction (MI) involves dual antiplatelet therapy (DAPT) with ...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"6 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141509900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures","authors":"Caiting Yang, Jie Han, Hai Liu, Yuyu He, Zhenhua Zhang, Xiaochun Liu, Farooq Waqas, Lizhong Zhang, Huiping Duan, Jing He, Li Dong","doi":"10.1080/09537104.2024.2337255","DOIUrl":"https://doi.org/10.1080/09537104.2024.2337255","url":null,"abstract":"Exosomes carry large cargo of proteins, lipids, and nucleic acids, serving as versatile biomarkers for disease diagnosis and vehicles for drug delivery. However, up to date, no well recognized stan...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"14 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140617886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of AMPK in platelets promotes the production of offspring","authors":"Tong Zhang, Mengnan Yang, Shujun Li, Rong Yan, Kesheng Dai","doi":"10.1080/09537104.2024.2334701","DOIUrl":"https://doi.org/10.1080/09537104.2024.2334701","url":null,"abstract":"Platelets are terminally differentiated anucleated cells, but they still have cell-like functions and can even produce progeny platelets. However, the mechanism of platelet sprouting has not been e...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"69 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The bile acid receptor TGR5 inhibits platelet activation and thrombus formation","authors":"Friedrich Reusswig, Maria Reich, Leonard Wienands, Diran Herebian, Verena Keitel-Anselmino, Margitta Elvers","doi":"10.1080/09537104.2024.2322733","DOIUrl":"https://doi.org/10.1080/09537104.2024.2322733","url":null,"abstract":"Liver failure and cirrhosis are characterized by abnormal hemostasis with aberrant platelet activation. In particular, the consequences of cholestatic liver disease and molecular mechanisms, includ...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"2018 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The natural anticoagulant protein S; hemostatic functions and deficiency","authors":"Fahad S. Alshehri, Abdullah A. Bashmeil, Ibrahim A. Alamar, Sarah K. Alouda","doi":"10.1080/09537104.2024.2337907","DOIUrl":"https://doi.org/10.1080/09537104.2024.2337907","url":null,"abstract":"Protein S (PS) is a vital endogenous anticoagulant. It plays a crucial role in regulating coagulation by acting as a cofactor for the activated protein C (APC) and tissue factor pathway inhibitor (...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"1 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlations among different platelet aggregation pathways in a group of healthy volunteers","authors":"Alejandro Carazo, Marcel Hrubša, Lukáš Konečný, Catherine Gunaseelan, Jaka Fadraersada, Pavel Skořepa, Markéta Paclíková, František Musil, Jana Karlíčková, Lenka Javorská, Kateřina Matoušová, Lenka Kujovská Krčmová, Alena Šmahelová, Vladimír Blaha, Přemysl Mladenka","doi":"10.1080/09537104.2024.2336093","DOIUrl":"https://doi.org/10.1080/09537104.2024.2336093","url":null,"abstract":"Platelet aggregation is a complicated process mediated by different signaling pathways. As the process is highly complex and apparently redundant, the relationships between these pathways are not y...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"69 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of VerifyNow to assess the immediate pharmacodynamic response of chewed and swallowed aspirin: comparison with aggregometry","authors":"Kevin P. Bliden, Parshotam Kundan, Danielle Kraft, Rueshil Parekh, Sahib Singh, Aravind D. Babu, Anika P. Shah, Udaya S. Tantry, Paul A. Gurbel","doi":"10.1080/09537104.2023.2298352","DOIUrl":"https://doi.org/10.1080/09537104.2023.2298352","url":null,"abstract":"VerifyNow (VN) test is a less laborious method to assess pharmacodynamics (PD) compared to light transmittance aggregometry (LTA). VN assay has not been used to study the immediate PD effects of ac...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"70 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139083186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}