Platelets最新文献

{"title":"A case-control study of bleeding risk in children with 22q11.2 deletion syndrome undergoing cardiac surgery","authors":"T. Blaine Crowley, Ian Campbell, Abinaya Arulselvan, David Friedman, Elaine H. Zackai, Tracy R. Geoffrion, Char Witmer, J. William Gaynor, Donna M. McDonald-McGinn, Michele P. Lambert","doi":"10.1080/09537104.2023.2290108","DOIUrl":"https://doi.org/10.1080/09537104.2023.2290108","url":null,"abstract":"Previous research suggests that individuals with 22q11.2 deletion syndrome (DS) have an increased risk of bleeding following cardiac surgery. However, current guidelines for management of patients ...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"49 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138688142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of platelet count in a murine stasis model of deep vein thrombosis","authors":"Rick Mathews, Naly Setthavongsack, Anh Le-Cook, Andy Kaempf, Jennifer M Loftis, Randall L Woltjer, Christina U Lorentz, Alexey Revenko, Monica T Hinds, Khanh P Nguyen","doi":"10.1080/09537104.2023.2290916","DOIUrl":"https://doi.org/10.1080/09537104.2023.2290916","url":null,"abstract":"Platelets are core components of thrombi but their effect on thrombus burden during deep vein thrombosis (DVT) has not been fully characterized. We examined the role of thrombopoietin-altered plate...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"23 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rapid change of shear rate gradient is beneficial to platelet activation","authors":"Tiancong Zhang, Ling Liu, Xiaojing Huang, Xuemei Gao, Xuanrong Huan, Cui He, Yuan Li","doi":"10.1080/09537104.2023.2288679","DOIUrl":"https://doi.org/10.1080/09537104.2023.2288679","url":null,"abstract":"Fluid shear plays a key role in hemostasis and thrombosis, and the purpose of this study was to investigate the effect of shear gradient change rate (SGCR) on platelet reactivity and von Willebrand...","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"4 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of apolipoprotein A-I as a target of platelet tyrosine kinases","authors":"Christine Föhrkolb, Katrin Vogel, Günter Lochnit, Peter Presek","doi":"10.1080/09537104.2023.2290921","DOIUrl":"https://doi.org/10.1080/09537104.2023.2290921","url":null,"abstract":"Published in Platelets (Vol. 35, No. 1, 2024)","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"264 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138688072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the therapeutic window of sirolimus by monitoring blood concentration for the treatment of immune thrombocytopenia.","authors":"Yun Zhang, Yao Quan, Dan Wang, Kaniel Cassady, Wenhang Zou, Jingkang Xiong, Han Yao, Xiaojuan Deng, Ping Wang, Shijie Yang, Xi Zhang, Yimei Feng","doi":"10.1080/09537104.2023.2277831","DOIUrl":"10.1080/09537104.2023.2277831","url":null,"abstract":"<p><p>Previous studies have demonstrated that sirolimus (SRL) is an effective agent for the treatment of refractory/relapsed (R/R) ITP. However, the therapeutic window of sirolimus in the treatment of ITP has not been established. As the toxicity of sirolimus increases with higher blood concentrations, it is crucial to determine the optimal therapeutic concentration of SRL for the treatment of ITP. Thus, in this study, we used a retrospective cohort of ITP patients treated with sirolimus to propose the therapeutic dosage window for sirolimus. A total of 275 laboratory results of SRL blood concentration from 63 ITP patients treated with SRL were analyzed retrospectively. The ITP patients were divided into five groups based on their SRL blood concentration: 0-4 ng/ml, 4-8 ng/ml, 8-12 ng/ml, 12-16 ng/ml and ≥16 ng/ml. In addition to the SRL blood concentration, platelet counts and adverse events that occurred during the first 6 weeks of SRL treatment were analyzed. These findings were then used to establish the decision matrix tables and ROC curves, which helped identify the therapeutic window of SRL. Based on the values and trends of true-positive rate (TPR) and false-positive rate (FPR) in the ROC curve, patients who achieved a SRL blood concentration of 4-12 ng/ml displayed a higher response rate compared to those with a SRL concentration of 0-4 ng/ml or ≥16ng/ml. Additionally, the response rate was better for patients with a SRL concentration of 8-12 ng/ml compared to 4-8 ng/ml. Adverse events were related to the concentration of SRL; however, there was no significant difference in the incidence of adverse events between the concentrations of 4-8 ng/ml and 8-12 ng/ml (<i>P </i>> .05). Regression analysis suggested that the concentration of SRL correlated with the patient's age, PLT count at the start of SRL administration, and the dose of SRL. It is suggested that the optimal blood concentration of SRL monotherapy for managing ITP is 8-12 ng/ml. This range may achieve a favorable balance between clinical efficacy and the severity of adverse events.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"34 1","pages":"2277831"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends among platelet function, arterial calcium, and vascular function measures.","authors":"Jason Cunha, Melissa V Chan, Bongani B Nkambule, Florian Thibord, Amber Lachapelle, Robin E Pashek, Ramachandran S Vasan, Jian Rong, Emelia J Benjamin, Naomi M Hamburg, Ming-Huei Chen, Gary F Mitchell, Andrew D Johnson","doi":"10.1080/09537104.2023.2238835","DOIUrl":"10.1080/09537104.2023.2238835","url":null,"abstract":"<p><p>Arterial tonometry and vascular calcification measures are useful in cardiovascular disease (CVD) risk assessment. Prior studies found associations between tonometry measures, arterial calcium, and CVD risk. Activated platelets release angiopoietin-1 and other factors, which may connect vascular structure and platelet function. We analyzed arterial tonometry, platelet function, aortic, thoracic and coronary calcium, and thoracic and abdominal aorta diameters measured in the Framingham Heart Study Gen3/NOS/OMNI-2 cohorts (<i>n</i> = 3,429, 53.7% women, mean age 54.4 years ±9.3). Platelet reactivity in whole blood or platelet-rich plasma was assessed using 5 assays and 7 agonists. We analyzed linear mixed effects models with platelet reactivity phenotypes as outcomes, adjusting for CVD risk factors and family structure. Higher arterial calcium trended with higher platelet reactivity, whereas larger aortic diameters trended with lower platelet reactivity. Characteristic impedance (Zc) and central pulse pressure positively trended with various platelet traits, while pulse wave velocity and Zc negatively trended with collagen, ADP, and epinephrine traits. All results did not pass a stringent multiple test correction threshold (<i>p</i> < 2.22e-04). The diameter trends were consistent with lower shear environments invoking less platelet reactivity. The vessel calcium trends were consistent with subclinical atherosclerosis and platelet activation being inter-related.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"34 1","pages":"2238835"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10947606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10283159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humanized mouse models for inherited thrombocytopenia studies.","authors":"Xiaojie Wang,&nbsp;Maoshan Chen,&nbsp;Lanyue Hu,&nbsp;Chengning Tan,&nbsp;Xiaoliang Li,&nbsp;Peipei Xue,&nbsp;Yangzhou Jiang,&nbsp;Peipei Bao,&nbsp;Teng Yu,&nbsp;Fengjie Li,&nbsp;Yanni Xiao,&nbsp;Qian Ran,&nbsp;Zhongjun Li,&nbsp;Li Chen","doi":"10.1080/09537104.2023.2267676","DOIUrl":"10.1080/09537104.2023.2267676","url":null,"abstract":"<p><p>Inherited thrombocytopenia (IT) is a group of hereditary disorders characterized by a reduced platelet count as the main clinical manifestation, and often with abnormal platelet function, which can subsequently lead to impaired hemostasis. In the past decades, humanized mouse models (HMMs), that are mice engrafted with human cells or genes, have been widely used in different research areas including immunology, oncology, and virology. With advances of the development of immunodeficient mice, the engraftment, and reconstitution of functional human platelets in HMM permit studies of occurrence and development of platelet disorders including IT and treatment strategies. This article mainly reviews the development of humanized mice models, the construction methods, research status, and problems of using humanized mice for the <i>in vivo</i> study of human thrombopoiesis.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"34 1","pages":"2267676"},"PeriodicalIF":3.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41237990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atorvastatin-mediated inhibition of prenylation of Rab27b and Rap1a in platelets attenuates their prothrombotic capacity and modulates clot structure.","authors":"Mohammed M Jalal, Claire S Whyte, Fraser P Coxon, Nicola J Mutch","doi":"10.1080/09537104.2023.2206921","DOIUrl":"10.1080/09537104.2023.2206921","url":null,"abstract":"<p><p>Statins inhibit the mevalonate pathway by impairing protein prenylation via depletion of lipid geranylgeranyl diphosphate (GGPP). Rab27b and Rap1a are small GTPase proteins involved in dense granule secretion, platelet activation, and regulation. We analyzed the impact of statins on prenylation of Rab27b and Rap1a in platelets and the downstream effects on fibrin clot properties. Whole blood thromboelastography revealed that atorvastatin (ATV) delayed clot formation time (<i>P</i> < .005) and attenuated clot firmness (<i>P</i> < .005). ATV pre-treatment inhibited platelet aggregation and clot retraction. Binding of fibrinogen and P-selectin exposure on stimulated platelets was significantly lower following pre-treatment with ATV (<i>P</i> < .05). Confocal microscopy revealed that ATV significantly altered the structure of platelet-rich plasma clots, consistent with the reduced fibrinogen binding. ATV enhanced lysis of Chandler model thrombi 1.4-fold versus control (<i>P</i> < .05). Western blotting revealed that ATV induced a dose-dependent accumulation of unprenylated Rab27b and Rap1a in the platelet membrane. ATV dose-dependently inhibited ADP release from activated platelets. Exogenous GGPP rescued the prenylation of Rab27b and Rap1a, and partially restored the ADP release defect, suggesting these changes arise from reduced prenylation of Rab27b. These data demonstrate that statins attenuate platelet aggregation, degranulation, and binding of fibrinogen thereby having a significant impact on clot contraction and structure.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"34 1","pages":"2206921"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemostasis-on-a-chip / incorporating the endothelium in microfluidic models of bleeding.","authors":"Yumiko Sakurai, Elaissa T Hardy, Wilbur A Lam","doi":"10.1080/09537104.2023.2185453","DOIUrl":"10.1080/09537104.2023.2185453","url":null,"abstract":"<p><p>Currently, point-of-care assays for human platelet function and coagulation are used to assess bleeding risks and drug testing, but they lack intact endothelium, a critical component of the human vascular system. Within these assays, the assessment of bleeding risk is typically indicated by the lack of or reduced platelet function and coagulation without true evaluation of hemostasis. Hemostasis is defined as the cessation of bleeding. Additionally, animal models of hemostasis also, by definition, lack human endothelium, which may limit their clinical relevance. This review discusses the current state-of-the-art of hemostasis-on-a-chip, specifically, human cell-based microfluidic models that incorporate endothelial cells, which function as physiologically relevant <i>in vitro</i> models of bleeding. These assays recapitulate the entire process of vascular injury, bleeding, and hemostasis, and provide real-time, direct observation, thereby serving as research-enabling tools that enhance our understanding of hemostasis and also as novel drug discovery platforms.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"34 1","pages":"2185453"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10197822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9493322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization of spleen tyrosine kinase (SYK) isoforms in platelets.","authors":"Pankaj Kumar Singh, Carol A Dangelmaier, Hymavathi Reddy Vari, Alexander Y Tsygankov, Satya P Kunapuli","doi":"10.1080/09537104.2023.2249549","DOIUrl":"10.1080/09537104.2023.2249549","url":null,"abstract":"<p><p>Alternate splicing is among the regulatory mechanisms imparting functional diversity in proteins. Studying protein isoforms generated through alternative splicing is therefore critical for understanding protein functions in many biological systems. Spleen tyrosine kinase (Syk) plays an essential role in ITAM/hemITAM signaling in many cell types, including platelets. However, the spectrum of Syk isoforms expressed in platelets has not been characterized. Syk has been shown to have a full-length long isoform SykL and a shorter SykS lacking 23 amino acid residues within its interdomain B. Furthermore, putative isoforms lacking another 23 amino acid-long sequence or a combination of the two deletions have been postulated to exist. In this report, we demonstrate that mouse platelets express full-length SykL and the previously described shorter isoform SykS, but lack other shorter isoforms, whereas human platelets express predominantly SykL. These results both indicate a possible role of alternative <i>Syk</i> splicing in the regulation of receptor signaling in mouse platelets and a difference between signaling regulation in mouse and human platelets.</p>","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"34 1","pages":"2249549"},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10607793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}