Plant Health Progress最新文献_第8页

Xylella fastidiosa not detected on tree crops after five years of survey 经过5年的调查，在树木作物上未发现苛养木杆菌

IF 2.3

Plant Health Progress Pub Date : 2023-01-18 DOI: 10.1094/php-11-22-0120-rs

N. Al-Karablieh, Ibtihal AbuObeid, Lina Al-Elaumi, Ihsan Mutlaq, J. Haddadin, Ruba Al Omari, Abdel-Munem Al-Jabaree, Safa Mazahreh

{"title":"Xylella fastidiosa not detected on tree crops after five years of survey","authors":"N. Al-Karablieh, Ibtihal AbuObeid, Lina Al-Elaumi, Ihsan Mutlaq, J. Haddadin, Ruba Al Omari, Abdel-Munem Al-Jabaree, Safa Mazahreh","doi":"10.1094/php-11-22-0120-rs","DOIUrl":"https://doi.org/10.1094/php-11-22-0120-rs","url":null,"abstract":"With the X. fastidiosa outbreak in Europe affecting olive and other major crops, the Jordanian Ministry of Agriculture (MoA) signaled a red warning light to prevent its entry into Jordan. An intensive survey was performed during 2016-2021 to assess its spread in Jordan across a range of agricultural crops in parallel to the previously published survey on olives. Grapevine (no. of samples: 899), stone fruit trees (1480), citrus fruit trees (1225), pome fruit trees (292), and ornamentals plants (1351) growing in Jordan were sampled. Both symptomatic and asymptomatic plants were sampled, in addition to collecting potential insect vectors from Hemiptera species. Plant samples were tested by ELISA (Enzyme-Linked Immunosorbent Assay) kits and their results were confirmed by conventional PCR (Polymerase Chain Reaction) using three sets of primers. Insect samples were tested using RST31/RST33 PCR. The obtained results did not show any confirmed positive results in any sample. These results indicate that X. fastidiosa has not been detected in Jordan despite a comprehensive survey. These results also demonstrate the importance of the monitoring and inspection programs executed by the MoA for detection of X. fastidiosa and identifying its potential insect vectors; these programs should be continued to prevent the entry of this bacterium and its potential insect vectors into Jordan from the neighboring countries.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48874194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex PCR assay for rapid identification of Monilinia rubi, the causal agent of dry-berry disease of caneberries 用多重PCR方法快速鉴定引起蔓越莓干果病的病原菌红门菌

IF 2.3

Plant Health Progress Pub Date : 2023-01-13 DOI: 10.1094/php-09-22-0095-br

W. Weldon, G. McGhee, Jeffery A. Delong, V. Stockwell

{"title":"Multiplex PCR assay for rapid identification of Monilinia rubi, the causal agent of dry-berry disease of caneberries","authors":"W. Weldon, G. McGhee, Jeffery A. Delong, V. Stockwell","doi":"10.1094/php-09-22-0095-br","DOIUrl":"https://doi.org/10.1094/php-09-22-0095-br","url":null,"abstract":"Monilinia rubi is the causal agent of dry-berry disease of raspberry and blackberry in northern Washington state and western Canada. The symptoms are visible on green fruits and include necrotic and dried drupelets with progressive necrosis from the receptacle into the peduncle. Diagnosis is based on symptoms and isolation and identification of the slow-growing fungal pathogen. Diagnosis is slow and difficult with late season tissues because abiotic stresses or other diseases may cause similar symptoms and the slow-growing pathogen is not easily isolated from tissues harboring fast-growing environmental fungi. A multiplex PCR assay with primers to amplify an ITS region and beta-tubulin was designed to provide a rapid method to identify the pathogen in culture and in infected berry tissues. For M. rubi and infected berries, two amplicons that differ in length by 400 bp are visualized on agarose gels. No bands were obtained from fungal outgroups or non-symptomatic berries. For further confirmation of the pathogen and the disease, a single amplicon can be sequenced directly from the multiplex reaction and compared to reference sequences in GenBank. This rapid multiplex assay streamlines diagnosis of dry-berry disease and its application may provide valuable information on the range of the pathogen, especially in other caneberry production regions.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44269422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of Botrytis cinerea in Petunia Using Cultivar Resistance and Biorational Products 利用品种抗性和生物制品防治矮牵牛花灰霉病

IF 2.3

Plant Health Progress Pub Date : 2023-01-12 DOI: 10.1094/php-11-22-0118-rs

S. Shrestha, M. Hausbeck

{"title":"Management of Botrytis cinerea in Petunia Using Cultivar Resistance and Biorational Products","authors":"S. Shrestha, M. Hausbeck","doi":"10.1094/php-11-22-0118-rs","DOIUrl":"https://doi.org/10.1094/php-11-22-0118-rs","url":null,"abstract":"Botrytis cinerea causes blight on the leaves, stems, and flowers of petunia (Petunia x hybrida), a popular annual bedding plant. Our objectives were to evaluate: (i) four standard and nine Wave® petunia cultivars for resistance to Botrytis blight and (ii) ten biorational products compared to the standard fungicide fenhexamid and an untreated control to limit disease. According to the final disease assessment and AUDPC data, ‘Sophistica Blackberry’ had significantly less disease than ‘Tidal Wave® Cherry’ in all trials. ‘Tidal Wave® Cherry’ also had significantly more disease according to ratings and AUDPC data than ‘Easy Wave® Red Improved’, ‘Wave® Purple Classic’, ‘Tidal Wave® Silver’, ‘Shock Wave® Red’ and ‘Easy Wave® Blue’ in trials 1 and 3, whereas in trial 2 these differences were not observed. When evaluated on ‘Shock Wave® Red’ petunia, Prestop (Gliocladium catenulatum) provided significant B. cinerea control and was similar to the fungicide standard Decree (fenhexamid) in both trials. According to the final disease severity assessment, treatment with Botector (Aureobasidium pullulans), Zio (Pseudomonas chlororaphis) or LifeGard (Bacillus mycoides) also provided control similar to the fungicide standard but was significantly different from the untreated control in one trial only. Results from this study illustrate that certain biorational products can limit B. cinerea when used in conjunction with a cultivar that has disease resistance that limits the impact of the pathogen. Keywords: Petunia x hybrida, gray mold, fenhexamid, fungicide","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46867107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Metconazole + Prothioconazole, Pydiflumetofen + Propiconazole, and Variety Resistance on Fusarium Head Blight and Yield in Hard Red Spring Wheat Metconazole + Prothioconazole、Pydiflumetofen + Propiconazole及品种抗病性对硬红春小麦赤霉病及产量的影响

IF 2.3

Plant Health Progress Pub Date : 2023-01-12 DOI: 10.1094/php-08-22-0070-rs

LeAnn Lux, Jessica M. Halvorson, Bryan Hansen, Scott M. Meyer, Scott Fitterer, David Carruth, A. Friskop

{"title":"Effect of Metconazole + Prothioconazole, Pydiflumetofen + Propiconazole, and Variety Resistance on Fusarium Head Blight and Yield in Hard Red Spring Wheat","authors":"LeAnn Lux, Jessica M. Halvorson, Bryan Hansen, Scott M. Meyer, Scott Fitterer, David Carruth, A. Friskop","doi":"10.1094/php-08-22-0070-rs","DOIUrl":"https://doi.org/10.1094/php-08-22-0070-rs","url":null,"abstract":"Fusarium head blight (FHB), caused primarily by Fusarium graminearum (Schwabe), is one of the most significant diseases of hard red spring wheat (HRSW) in North Dakota (ND). Implementing integrated management practices such as host resistance and fungicides can be used to manage FHB and the associated mycotoxin deoxynivalenol (DON). However, depending on the production year in ND, both management tools may not be needed for FHB. The objectives of this research were to evaluate fungicide premixes and varietal resistance on suppression of FHB, suppression of DON, and economic response in diverse FHB environments in dryland HRSW. Seven field experiments were conducted at dryland locations from 2019 to 2021. Two HRSW varieties, WB-Mayville (susceptible) and ND-VitPro (moderately resistant) served as the main plots and seven fungicide treatments including a non-treated control served as the sub-plots. Based on disease levels and local weather data (relative humidity, rainfall, and temperature) field experiments were categorized into four FHB management environments (high, moderate, low and no disease) to assess effectiveness of management tools in field situations faced by HRSW growers. The fungicide premixture treatments of metconazole + prothioconazole and pydiflumetofen + propiconazole provided similar or better suppression of FHB and DON when compared to FHB fungicide standards. Results suggest that the use of fungicides on a moderately resistant variety provided a more favorable economic response when compared to their use on a susceptible variety in moderate and low FHB environments. Results from this study will help guide FHB management decisions in dryland HRSW.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":"1 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"61310918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Late-Season Decline – A New Bacterial Disease of Corn Identified in the Texas Panhandle 晚季衰退——在得克萨斯州狭长地带发现的一种新的玉米细菌性疾病

IF 2.3

Plant Health Progress Pub Date : 2023-01-09 DOI: 10.1094/php-10-22-0106-rs

Ken Obasa, M. Kolomiets, B. Reed, D. Coker, J. Bell, K. Heflin

{"title":"Late-Season Decline – A New Bacterial Disease of Corn Identified in the Texas Panhandle","authors":"Ken Obasa, M. Kolomiets, B. Reed, D. Coker, J. Bell, K. Heflin","doi":"10.1094/php-10-22-0106-rs","DOIUrl":"https://doi.org/10.1094/php-10-22-0106-rs","url":null,"abstract":"The genus Pantoea has historically been associated with two diseases of corn, Stewart’s wilt caused by P. stewartii, and necrotic or white leaf spots or streaks and stalk rot caused by P. ananatis. In 2020 and 2021, a sudden and unusual decline of corn stands was observed in corn fields in two counties in the Texas High Plains region. Symptoms observed included an initial light green, elongate, slightly translucent, and non-chlorotic streaked lesions with non-wavy margins that developed on leaf blades during corn vegetative growth stages, with lesions becoming necrotic at the onset of crop reproduction. Additionally, stunting of affected plants, poor ear development, and stalk rot were associated with affected stands. Diagnosis of symptomatic tissues consistently recovered bacteria. BLAST searches of the partial 16S rRNA sequences of the bacterial isolates identified them as belonging to the genus Pantoea. Investigations of the pathogenicity of two bacterial isolates, B566 and B623, under greenhouse conditions relying on Koch’s postulates resulted in the development of symptoms identical to those observed on symptomatic field corn plants. The two bacteria were also successfully recovered from symptomatic leaf and stem tissues, thus satisfying Koch’s postulates. Sequence analysis showed these isolates are closely related to P. ananatis, but also phylogenetically distinct. Findings from this study provide evidence for a new disease of corn caused by two Pantoea species that can result in stand decline of infected corn plants.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48311204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viromes of field-grown tomatoes and peppers in Tennessee revealed by RNA sequencing followed by bioinformatic analysis 通过RNA测序和生物信息学分析揭示了田纳西州田间种植的番茄和辣椒的病毒体

IF 2.3

Plant Health Progress Pub Date : 2023-01-06 DOI: 10.1094/php-10-22-0107-rs

N. Dias, Rongbin Hu, F. Hale, Z. Hansen, A. Wszelaki, L. Domier, M. R. Hajimorad

{"title":"Viromes of field-grown tomatoes and peppers in Tennessee revealed by RNA sequencing followed by bioinformatic analysis","authors":"N. Dias, Rongbin Hu, F. Hale, Z. Hansen, A. Wszelaki, L. Domier, M. R. Hajimorad","doi":"10.1094/php-10-22-0107-rs","DOIUrl":"https://doi.org/10.1094/php-10-22-0107-rs","url":null,"abstract":"The prevalent viruses in a population of 106 tomato and 53 pepper field-grown plants in Tennessee (TN) was investigated by RNA sequencing and analysis as two separate pools of ribosomal-depleted total RNA. Contigs of potato leafroll virus (PLRV), red clover vein mosaic virus, tomato spotted wilt virus and a novel rhabdovirus were detected only in tomato and those of bell pepper endornavirus (BPEV), and pepper cryptic virus 2 (PCV-2) only in pepper. Additionally, contigs of five other viruses (solanum nigrum ilarvirus 1; SNIV-1, southern tomato virus; STV, tobacco vein-clearing virus; ToVCV, tomato mosaic virus; ToMV and tomato ringspot virus; ToRSV) were detected in both tomatoes and peppers. Moreover, contigs were assembled of a potentially novel tobamovirus from both tomatoes and peppers. The presence of PLRV, STV, ToVCV, and the novel tobamovirus sequences in the original tomato samples and those of BPEV, PCV-2, ToMV, and ToRSV in the original pepper samples were confirmed by RT-PCR followed by Sanger sequencing. This study represents the first survey of viromes of field-grown tomatoes and peppers in TN. To the best of our knowledge, SNIV-1, STV and ToVCV have not been reported previously associated with peppers elsewhere in the world.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47160968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of sources of resistance to clubroot (Plasmodiophora brassicae) in a collection of Brassica napus plant introductions 甘蓝型引种植物棒根病抗性来源鉴定

IF 2.3

Plant Health Progress Pub Date : 2023-01-03 DOI: 10.1094/php-09-22-0094-rs

D. Marino, K. Chittem, F. Shahoveisi, V. Chapara, Susan Ruud, L. E. del Río Mendoza

引用次数: 1

Strawberry Cultivar Susceptibility to Neopestalotiopsis leaf spot in Indiana 印第安纳州草莓品种对新香蒜霉叶斑病的易感性

IF 2.3

Plant Health Progress Pub Date : 2023-01-03 DOI: 10.1094/php-05-22-0049-rs

W. Guan, J. Bonkowski, T. Creswell, D. Egel

引用次数: 0

Isolates of Phytophthora sansomeana Display a Range of Aggressiveness on Soybean Seedlings 大豆疫霉分离株对大豆幼苗具有一定的侵袭性

IF 2.3

Plant Health Progress Pub Date : 2022-12-22 DOI: 10.1094/php-08-22-0075-rs

Linda Hebb, C. Bradley, D. Telenko, K. Wise, A. Dorrance

{"title":"Isolates of Phytophthora sansomeana Display a Range of Aggressiveness on Soybean Seedlings","authors":"Linda Hebb, C. Bradley, D. Telenko, K. Wise, A. Dorrance","doi":"10.1094/php-08-22-0075-rs","DOIUrl":"https://doi.org/10.1094/php-08-22-0075-rs","url":null,"abstract":"Soilborne oomycete pathogens of soybean are a constant concern, especially at the seedling growth stage. In a survey for Phytophthora sojae, 126 isolates of Phytophthora sansomeana were recovered through soil bating between 2016 and 2018, of which 42 were from Indiana, 9 from Kentucky, and 75 from Ohio. A subset of P. sansomeana isolates along with check isolates of Phytophthora sojae, Pythium irregulare, and Pythium ultimum var ultimum were selected for pathogenicity testing using Petri plate, tray test, and greenhouse cup assays. The average seed rot score for P. sansomeana infected seeds in the Petri plate assay was 2.3 on a scale from zero to three with three indicating complete colonization with little to no germination of seeds. In the tray test assay, the mean lesion development on the seedling tap root for P. sansomeana was 4.9 mm, compared to 46.0 mm for P. sojae. In the greenhouse cup assay the average root rot score of P. sansomeana was 2.1 on a scale from one to five where a score of five indicates no germination. Based on these assays, P. sansomeana isolates were more aggressive seed rotters than P. sojae and Pythium isolates. Conversely, they developed smaller tap root lesions on soybean seedlings than P. sojae, and less severe root rot compared with isolates of Pythium irregulare and Py. ultimum. This research reinforces the importance of selecting a diverse panel of isolates to screen for resistance in host plants, as well as selecting effective management strategies against multiple seedling pathogens.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45508190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of late-season soilborne pathogens infecting strawberry in Watsonville-Salinas, California 加州沃特森维尔-萨利纳斯草莓晚季土传病原菌调查

IF 2.3

Plant Health Progress Pub Date : 2022-12-21 DOI: 10.1094/php-06-22-0056-s

Mary Elizabeth Steele, S. Hewavitharana, Peter M. Henry, P. Goldman, G. Holmes

{"title":"Survey of late-season soilborne pathogens infecting strawberry in Watsonville-Salinas, California","authors":"Mary Elizabeth Steele, S. Hewavitharana, Peter M. Henry, P. Goldman, G. Holmes","doi":"10.1094/php-06-22-0056-s","DOIUrl":"https://doi.org/10.1094/php-06-22-0056-s","url":null,"abstract":"There are four major soilborne pathogens of strawberries in California, but their distribution and prevalence in the Watsonville-Salinas production district are unknown. To fill this knowledge gap, 74 symptomatic strawberry plant samples were collected from 69 fields in the Watsonville-Salinas growing district between 11 August and 15 October 2021. Each sample consisted of eight plants exhibiting moderate to severe plant collapse. Crown tissue from each plant was excised and pooled for recombinase polymerase amplification (RPA) to detect Macrophomina phaseolina, Fusarium oxysporum f. sp. fragariae, Verticillium dahliae, and Phytophthora spp. Root, petiole and crown tissue from plant samples in which no pathogens were detected by RPA was plated on semi-selective media to verify the absence of the four pathogens and screen for other pathogenic fungi. At least one of the four pathogens was detected in 55 of the 74 samples (74.3%). All four of the major soilborne pathogens are prevalent in this growing district, as F. oxysporum f. sp. fragariae, M. phaseolina, Phytophthora spp. and V. dahliae were detected in 23 samples (31.1%), 22 samples (29.7%), 18 samples (24.3%), and 16 samples (22.0%), respectively. No strong associations were found between the pathogens and growing practices.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46970971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0