Viromes of field-grown tomatoes and peppers in Tennessee revealed by RNA sequencing followed by bioinformatic analysis

IF 1.7 Q2 PLANT SCIENCES

Plant Health Progress Pub Date : 2023-01-06 DOI:10.1094/php-10-22-0107-rs

N. Dias, Rongbin Hu, F. Hale, Z. Hansen, A. Wszelaki, L. Domier, M. R. Hajimorad

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引用次数: 1

Abstract

The prevalent viruses in a population of 106 tomato and 53 pepper field-grown plants in Tennessee (TN) was investigated by RNA sequencing and analysis as two separate pools of ribosomal-depleted total RNA. Contigs of potato leafroll virus (PLRV), red clover vein mosaic virus, tomato spotted wilt virus and a novel rhabdovirus were detected only in tomato and those of bell pepper endornavirus (BPEV), and pepper cryptic virus 2 (PCV-2) only in pepper. Additionally, contigs of five other viruses (solanum nigrum ilarvirus 1; SNIV-1, southern tomato virus; STV, tobacco vein-clearing virus; ToVCV, tomato mosaic virus; ToMV and tomato ringspot virus; ToRSV) were detected in both tomatoes and peppers. Moreover, contigs were assembled of a potentially novel tobamovirus from both tomatoes and peppers. The presence of PLRV, STV, ToVCV, and the novel tobamovirus sequences in the original tomato samples and those of BPEV, PCV-2, ToMV, and ToRSV in the original pepper samples were confirmed by RT-PCR followed by Sanger sequencing. This study represents the first survey of viromes of field-grown tomatoes and peppers in TN. To the best of our knowledge, SNIV-1, STV and ToVCV have not been reported previously associated with peppers elsewhere in the world.

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通过RNA测序和生物信息学分析揭示了田纳西州田间种植的番茄和辣椒的病毒体

通过RNA测序和分析，作为核糖体缺失的总RNA的两个独立库，对田纳西州106株番茄和53株辣椒田间种植植物中的流行病毒进行了研究。仅在番茄中检测到马铃薯卷叶病毒（PLRV）、红三叶叶脉花叶病毒、番茄斑点枯萎病病毒和一种新的弹状病毒，仅在辣椒中检测到甜椒内病毒（BPEV）和辣椒隐性病毒2型（PCV-2）。此外，在番茄和辣椒中还检测到其他五种病毒的重叠群（龙葵病毒1；SNIV-1，南部番茄病毒；STV，烟草清脉病毒；ToVCV，番茄花叶病毒；ToMV和番茄环斑病毒；ToRSV）。此外，一种潜在的新型烟草病毒从番茄和辣椒中组合而成。通过RT-PCR和Sanger测序证实了PLRV、STV、ToVCV和新的烟草病毒序列在原始番茄样品中的存在，以及BPEV、PCV-2、ToMV和ToRSV在原始辣椒样品中的出现。这项研究首次对田纳西州田间种植的番茄和辣椒的病毒群进行了调查。据我们所知，SNIV-1、STV和ToVCV以前从未与世界其他地方的辣椒有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Health Progress Agricultural and Biological Sciences-Horticulture

CiteScore

3.00

自引率

13.00%

发文量

期刊介绍： Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.