Survey of late-season soilborne pathogens infecting strawberry in Watsonville-Salinas, California

IF 1.7 Q2 PLANT SCIENCES

Plant Health Progress Pub Date : 2022-12-21 DOI:10.1094/php-06-22-0056-s

Mary Elizabeth Steele, S. Hewavitharana, Peter M. Henry, P. Goldman, G. Holmes

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Abstract

There are four major soilborne pathogens of strawberries in California, but their distribution and prevalence in the Watsonville-Salinas production district are unknown. To fill this knowledge gap, 74 symptomatic strawberry plant samples were collected from 69 fields in the Watsonville-Salinas growing district between 11 August and 15 October 2021. Each sample consisted of eight plants exhibiting moderate to severe plant collapse. Crown tissue from each plant was excised and pooled for recombinase polymerase amplification (RPA) to detect Macrophomina phaseolina, Fusarium oxysporum f. sp. fragariae, Verticillium dahliae, and Phytophthora spp. Root, petiole and crown tissue from plant samples in which no pathogens were detected by RPA was plated on semi-selective media to verify the absence of the four pathogens and screen for other pathogenic fungi. At least one of the four pathogens was detected in 55 of the 74 samples (74.3%). All four of the major soilborne pathogens are prevalent in this growing district, as F. oxysporum f. sp. fragariae, M. phaseolina, Phytophthora spp. and V. dahliae were detected in 23 samples (31.1%), 22 samples (29.7%), 18 samples (24.3%), and 16 samples (22.0%), respectively. No strong associations were found between the pathogens and growing practices.

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加州沃特森维尔-萨利纳斯草莓晚季土传病原菌调查

加利福尼亚州草莓有四种主要的土传病原体，但它们在Watsonville Salinas产区的分布和流行情况尚不清楚。为了填补这一知识空白，在2021年8月11日至10月15日期间，从Watsonville Salinas种植区的69块田地中采集了74份有症状的草莓植物样本。每个样本由八种表现出中度至重度植物塌陷的植物组成。将每株植物的冠组织切除并合并用于重组酶聚合酶扩增（RPA），以检测Macrophomina phaseolina、Fusarium oxysporum f.sp.fragariae、Verticillium dahliae和Phytophthora sp.根，将RPA未检测到病原体的植物样品的叶柄和树冠组织置于半选择性培养基上，以验证四种病原体的不存在，并筛选其他病原真菌。在74个样本中的55个样本（74.3%）中，至少检测到了这四种病原体中的一种。所有四种主要的土传病原体都在该生长区流行，分别在23个样本（31.1%）、22个样本（29.7%）、18个样本（24.3%）和16个样本（22.0%）中检测到了尖孢镰刀菌（F.oxysporum F.sp.fragariae）、相球菌（M.phaseolina）、疫霉菌（Phytophthora spp.）和大丽花（V.dahliae）。没有发现病原体与种植实践之间有强烈的关联。

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来源期刊

Plant Health Progress Agricultural and Biological Sciences-Horticulture

CiteScore

3.00

自引率

13.00%

发文量

期刊介绍： Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.