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Purification and properties of a chloroplastic phosphatase distinct from fructose bisphosphatase 一种不同于果糖双磷酸酶的叶绿体磷酸酶的纯化和性质
Plant Science Letters Pub Date : 1984-09-01 DOI: 10.1016/0304-4211(84)90248-7
Brigitte Gontero, Jean-Claude Meunier, Jacques Ricard
{"title":"Purification and properties of a chloroplastic phosphatase distinct from fructose bisphosphatase","authors":"Brigitte Gontero,&nbsp;Jean-Claude Meunier,&nbsp;Jacques Ricard","doi":"10.1016/0304-4211(84)90248-7","DOIUrl":"10.1016/0304-4211(84)90248-7","url":null,"abstract":"<div><p>A chloroplast phosphatase, distinct from fructose bisphosphatase, has been isolated and purified to apparent electrophoretic homogeneity from spinach leaves. This 100-kDa enzyme hydrolyzes both fructose and sedoheptulose bisphosphates. Given this lack of absolute substrate specificity, it cannot be considered a typical sedoheptulose bisphosphatase. The enzyme is reductively activated by reduced thioredoxin <em>f</em><sub><em>B</em></sub> in the presence of dithiothreitol (DTT), but not by thioredoxin <em>f</em><sub><em>A</em></sub> or thioredoxin <em>m</em>. Oxidized thioredoxin <em>f</em><sub><em>B</em></sub> deactivates the active reduced phosphatase.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90248-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84814974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
On the inhibiting effect of oligomycin on Mg: ATP-dependent ΔpH and Δψ in microsomal vesicles from radish 寡霉素对萝卜微粒体小泡中Mg: atp依赖性ΔpH和Δψ的抑制作用
Plant Science Letters Pub Date : 1984-09-01 DOI: 10.1016/0304-4211(84)90244-X
Maria Ida De Michelis, Franca Rasi-Caldogno, Maria Chiara Pugliarello
{"title":"On the inhibiting effect of oligomycin on Mg: ATP-dependent ΔpH and Δψ in microsomal vesicles from radish","authors":"Maria Ida De Michelis,&nbsp;Franca Rasi-Caldogno,&nbsp;Maria Chiara Pugliarello","doi":"10.1016/0304-4211(84)90244-X","DOIUrl":"10.1016/0304-4211(84)90244-X","url":null,"abstract":"<div><p>The kinetics of inhibition by oligomycin of the mitochondrial ATPase and the Mg: ATP-dependent electrogenic transport of protons were compared in membrane preparations from radish seedlings.</p><p>Oligomycin blocks the phosphohydrolyzing activity of mitochondrial ATPase (measured at pH 8.5) at 1 μg × mg<sup>−1</sup> prot and the ATP-synthase one (measured at pH 6.6) at 3 μg × mg<sup>−1</sup> prot.</p><p>Oligomycin at these concentrations does not influence Mg: ATP-dependent proton transport and Mg: ATP-dependent hyperpolarization of transmembrane electric potential difference (PD) in microsomal vesicles. However, an inhibiting effect of the drug becomes apparent at concentrations higher than 10 μg × mg<sup>−1</sup> prot, increasing with the increase of oligomycin concentration at least up to 240 μg × mg<sup>−1</sup> prot. Both the vanadate and the NO<sub>3</sub><sup>−</sup>-sensitive systems of Mg: ATP-dependent electrogenic transport of protons show similar sensitivity to oligomycin.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90244-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73637698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
In vivo acetylene inactivation of Chlorella nitrate reductase and its subsequent activation by blue light and nitrate 硝酸小球藻还原酶体内乙炔失活及其在蓝光和硝酸盐作用下的活化
Plant Science Letters Pub Date : 1984-09-01 DOI: 10.1016/0304-4211(84)90243-8
M.L Muñoz-Calvo , M Villarroya-Sanchez , M Rodríguez-López , P.J Aparicio
{"title":"In vivo acetylene inactivation of Chlorella nitrate reductase and its subsequent activation by blue light and nitrate","authors":"M.L Muñoz-Calvo ,&nbsp;M Villarroya-Sanchez ,&nbsp;M Rodríguez-López ,&nbsp;P.J Aparicio","doi":"10.1016/0304-4211(84)90243-8","DOIUrl":"10.1016/0304-4211(84)90243-8","url":null,"abstract":"<div><p>Acetylene inactivates in vitro the reduced form of <em>Chlorella</em> NADH-nitrate reductase. In vivo acetylene atmosphere promotes the inactivation of nitrate reductase when the cell cultures were free of nitrate, more effectively under red light than under blue light. In vivo reactivation of acetylene inactivated nitrate reductase is nitrate and light dependent. The reactivation was also achieved in cells kept in media containing tungstate substituting for molybdate. For this in situ reactivation, blue light was much more effective than red light.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90243-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90366462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
In vitro induction of capitula from cotyledons of Carthamus tinctorius (Safflower) 红花红花(Carthamus tinctorius)子叶体外诱导头状花序的研究
Plant Science Letters Pub Date : 1984-09-01 DOI: 10.1016/0304-4211(84)90253-0
G. Tejovathi, S.Y. Anwar
{"title":"In vitro induction of capitula from cotyledons of Carthamus tinctorius (Safflower)","authors":"G. Tejovathi,&nbsp;S.Y. Anwar","doi":"10.1016/0304-4211(84)90253-0","DOIUrl":"10.1016/0304-4211(84)90253-0","url":null,"abstract":"<div><p>In vitro capitula (head-inflorescence) induction was observed in two varieties of Safflower (<em>Carthamus tinctorius</em>) from the inner surface of the cotyledons. Complete blooming of florets in a capitulum was observed within 55–90 days after inoculation. The pollen fertility of in vitro florets ranged from 90–95%. Embryo development was normal and a few seeds were also recovered.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90253-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83312354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Effects of high temperature on RNA-synthesis during germination of maize (Zea mays L.) 高温对玉米萌发过程中rna合成的影响
Plant Science Letters Pub Date : 1984-07-01 DOI: 10.1016/0304-4211(84)90229-3
Graham J.P. Riley
{"title":"Effects of high temperature on RNA-synthesis during germination of maize (Zea mays L.)","authors":"Graham J.P. Riley","doi":"10.1016/0304-4211(84)90229-3","DOIUrl":"10.1016/0304-4211(84)90229-3","url":null,"abstract":"<div><p>Maize seeds imbibing at high temperatyres (above 37°C) germinate poorly, and their embryos show a low rate of protein-synthesis. Extracts of embryos imbibing at normal and high temperatures were able to translate exogenous mRNA, and poly (A) RNA obtained from them stimulated polypeptide synthesis by reticulocyte and maize cell-free extracts.</p><p>Embryos imbibing at 41°C incorporated insignificant amounts of [<sup>3</sup>H] adenine into poly (A) RNA and high M<sub><em>r</em></sub> rRNA. Autoradiography showed that little transcription occurred in these embryos. Chromatin remained aggregated throughout imbibition at this temperature.</p><p>The results suggest that synthesis of new mRNA is essential for germination to proceed. Poor germination of maize at high temperature is probably due to the inhibition of transcription.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90229-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78008853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Retention of resistance to the root-knot nematode, Meloidogyne incognita, by Lycopersicon plants reproduced through tissue culture 通过组织培养繁殖的番茄植株对根结线虫的抗性保持
Plant Science Letters Pub Date : 1984-07-01 DOI: 10.1016/0304-4211(84)90236-0
Mohammed Ammati , Toshio Murashige , Ivan J. Thomason
{"title":"Retention of resistance to the root-knot nematode, Meloidogyne incognita, by Lycopersicon plants reproduced through tissue culture","authors":"Mohammed Ammati ,&nbsp;Toshio Murashige ,&nbsp;Ivan J. Thomason","doi":"10.1016/0304-4211(84)90236-0","DOIUrl":"10.1016/0304-4211(84)90236-0","url":null,"abstract":"<div><p><em>Lycopersicon glandulosum, L. peruvianum</em> and <em>L. esculentum</em> plants reproduced adventitiously from cotyledon explants retained their resistance to the root-knot nematode, <em>Meloidogyne incognita</em>. This reaffirms the utility of tissue cultures for screening and isolating nematode-resistant variants.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90236-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83417649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Control of Chloroplastic NADP-malate dehydrogenase activity by thioredoxins 硫氧还毒素对叶绿体nadp -苹果酸脱氢酶活性的控制
Plant Science Letters Pub Date : 1984-07-01 DOI: 10.1016/0304-4211(84)90225-6
Nathalie Ferté, Jean-Claude Meunier, Paul Sauve, Jacques Ricard
{"title":"Control of Chloroplastic NADP-malate dehydrogenase activity by thioredoxins","authors":"Nathalie Ferté,&nbsp;Jean-Claude Meunier,&nbsp;Paul Sauve,&nbsp;Jacques Ricard","doi":"10.1016/0304-4211(84)90225-6","DOIUrl":"10.1016/0304-4211(84)90225-6","url":null,"abstract":"<div><p>Oxidized (inactive) chloroplastic NADP-malate dehydrogenase is 56-k Da homodimer, the two subunits of which are bound by a disulfide bridge. The enzyme is activated by three distinct chloroplast thioredoxins, thioredoxin <em>m</em>, <em>f</em><sub><em>A</em></sub> and <em>f</em><sub><em>B</em></sub>. When reduced by these proteins, the active enzyme is stable, whereas when reduced by dithiothreitol, activity is unstable. This result is apparently the consequence of aggregation of the reduced protein during prolonged incubation with dithiothreitol. At pH-values similar to those in the chloroplast stroma in the dark (pH 7), the reduced enzyme is still active.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90225-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89146475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea 山药细胞悬浮培养对培养基pH值的影响
Plant Science Letters Pub Date : 1984-07-01 DOI: 10.1016/0304-4211(84)90230-X
R.G. Butenko, A.Kh. Lipsky, N.D. Chernyak, H.C. Arya
{"title":"Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea","authors":"R.G. Butenko,&nbsp;A.Kh. Lipsky,&nbsp;N.D. Chernyak,&nbsp;H.C. Arya","doi":"10.1016/0304-4211(84)90230-X","DOIUrl":"10.1016/0304-4211(84)90230-X","url":null,"abstract":"<div><p>The effect of initial pH of the culture medium (pH<sub><em>i</em></sub>) on growth, changes in pH of culture medium (pH<sub><em>c</em></sub>) and respiration of <em>Dioscorea deltoidea</em> cells was studied in cycle of batch cultivation. At pH<sub><em>i</em></sub> 3.50 the lag phase of growth and respiration was about 24 h. Within the pH<sub><em>i</em></sub>-range of 4.30–6.15 no lag phase was observed, growth and respiration being similar. At pH<sub><em>i</em></sub>-values of 2.00–2.50 and 8.00 the cell population died. At pH 3.50–6.15 the level of pH<sub><em>c</em></sub> was shifted to 4.6–4.7 by cells within 10–48 h. We suppose that the pH-regulation at pH<sub><em>i</em></sub> range of 3.50–6.15 by the cells to a definite value of the pH<sub><em>c</em></sub> (4.6–4.7) ensures their normal growthy cycle.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90230-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78029934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Growth and nicotine content of tobacco callus cultures without organogenesis 无器官发生的烟草愈伤组织的生长和尼古丁含量
Plant Science Letters Pub Date : 1984-07-01 DOI: 10.1016/0304-4211(84)90232-3
M.Teresa Piñol, Javier Palazon, Manuel Serrano
{"title":"Growth and nicotine content of tobacco callus cultures without organogenesis","authors":"M.Teresa Piñol,&nbsp;Javier Palazon,&nbsp;Manuel Serrano","doi":"10.1016/0304-4211(84)90232-3","DOIUrl":"10.1016/0304-4211(84)90232-3","url":null,"abstract":"<div><p>Growth and nicotine production were measured in callus tissue without organogenesis, derived from the petiole of <em>Nicotiana tabacum</em> L. cv. Burley 21 cultured on Murashige-Skoog medium (MS medium), T. Murashige and F. Skoog, Physiol. Plant., 15 (1962) 473, containing auxin α-naphthaleneacetic acid (NAA) and kinetin,at 25°C in the dark. Experiments clearly demonstrated that in the presence of a constant amount of kinetin, it is the auxin concentration in the culture medium that controls the rate of nicotine synthesis which follows a course almost parallel to callus growth. The maximum accumulation of nicotine in the callus (0.16% of dry wt.) is slightly higher than in the intact plant. A cytological examination of the callus tissue showed that the greater degree of cellular differentiation which causes the loss of meristematic areas, inhibits alkaloid synthesis.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90232-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83702318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Intraspecific genetic variation in cytokinin-controlled shoot morphogenesis from tissue explants of Petunia hybrida 细胞分裂素控制的矮牵牛组织外植体芽形态发生的种内遗传变异
Plant Science Letters Pub Date : 1984-07-01 DOI: 10.1016/0304-4211(84)90235-9
Rachel C. Skvirsky , Maureen R. Hanson , Frederick M. Ausubel
{"title":"Intraspecific genetic variation in cytokinin-controlled shoot morphogenesis from tissue explants of Petunia hybrida","authors":"Rachel C. Skvirsky ,&nbsp;Maureen R. Hanson ,&nbsp;Frederick M. Ausubel","doi":"10.1016/0304-4211(84)90235-9","DOIUrl":"10.1016/0304-4211(84)90235-9","url":null,"abstract":"<div><p>In this paper we present the characterization of a differential morphogenetic response to cytokinin between two <em>Petunia</em> lines cultured in vitro. In particular, we report that: (1) two inbred cultivars of <em>Petunia hybrida</em> differed in the concentrations of cytokinin required to induce shoot morphogenesis from leaf explants and from stem epidermal explants in vitro. These differences were specific to the particular cytokinin utilized. (2) The F<sub>1</sub> hybrids of the two cultivars exhibited a biphasic morphogenetic response to cytokinin which was unlike that of either parent. (3) The cytokinin dose responses were similar in the reciprocal F<sub>1</sub> hybrids, indicating that the response is controlled primarily by Mendelian genes, rather than by cytoplasmic factors.</p></div>","PeriodicalId":20221,"journal":{"name":"Plant Science Letters","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-4211(84)90235-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87287094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
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