Intraspecific genetic variation in cytokinin-controlled shoot morphogenesis from tissue explants of Petunia hybrida

Rachel C. Skvirsky , Maureen R. Hanson , Frederick M. Ausubel
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引用次数: 16

Abstract

In this paper we present the characterization of a differential morphogenetic response to cytokinin between two Petunia lines cultured in vitro. In particular, we report that: (1) two inbred cultivars of Petunia hybrida differed in the concentrations of cytokinin required to induce shoot morphogenesis from leaf explants and from stem epidermal explants in vitro. These differences were specific to the particular cytokinin utilized. (2) The F1 hybrids of the two cultivars exhibited a biphasic morphogenetic response to cytokinin which was unlike that of either parent. (3) The cytokinin dose responses were similar in the reciprocal F1 hybrids, indicating that the response is controlled primarily by Mendelian genes, rather than by cytoplasmic factors.

细胞分裂素控制的矮牵牛组织外植体芽形态发生的种内遗传变异
在本文中,我们提出了在体外培养的两个矮牵牛系对细胞分裂素的不同形态发生反应的表征。我们特别报道了:(1)两个自交系矮牵牛叶片外植体和茎表皮外植体诱导芽形态发生所需的细胞分裂素浓度存在差异。这些差异是特定于所使用的特定细胞分裂素的。(2)两个品种的F1杂种对细胞分裂素表现出不同于亲本的双相形态发生反应。(3)细胞分裂素的剂量反应在互易F1杂交体中相似,表明这种反应主要由孟德尔基因控制,而不是细胞质因子。
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