In vivo acetylene inactivation of Chlorella nitrate reductase and its subsequent activation by blue light and nitrate

Acetylene inactivates in vitro the reduced form of Chlorella NADH-nitrate reductase. In vivo acetylene atmosphere promotes the inactivation of nitrate reductase when the cell cultures were free of nitrate, more effectively under red light than under blue light. In vivo reactivation of acetylene inactivated nitrate reductase is nitrate and light dependent. The reactivation was also achieved in cells kept in media containing tungstate substituting for molybdate. For this in situ reactivation, blue light was much more effective than red light.