On the inhibiting effect of oligomycin on Mg: ATP-dependent ΔpH and Δψ in microsomal vesicles from radish

Plant Science Letters Pub Date : 1984-09-01 DOI:10.1016/0304-4211(84)90244-X

Maria Ida De Michelis, Franca Rasi-Caldogno, Maria Chiara Pugliarello

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引用次数: 10

Abstract

The kinetics of inhibition by oligomycin of the mitochondrial ATPase and the Mg: ATP-dependent electrogenic transport of protons were compared in membrane preparations from radish seedlings.

Oligomycin blocks the phosphohydrolyzing activity of mitochondrial ATPase (measured at pH 8.5) at 1 μg × mg⁻¹ prot and the ATP-synthase one (measured at pH 6.6) at 3 μg × mg⁻¹ prot.

Oligomycin at these concentrations does not influence Mg: ATP-dependent proton transport and Mg: ATP-dependent hyperpolarization of transmembrane electric potential difference (PD) in microsomal vesicles. However, an inhibiting effect of the drug becomes apparent at concentrations higher than 10 μg × mg⁻¹ prot, increasing with the increase of oligomycin concentration at least up to 240 μg × mg⁻¹ prot. Both the vanadate and the NO₃⁻-sensitive systems of Mg: ATP-dependent electrogenic transport of protons show similar sensitivity to oligomycin.

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寡霉素对萝卜微粒体小泡中Mg: atp依赖性ΔpH和Δψ的抑制作用

在萝卜幼苗膜制备中，比较了寡霉素对线粒体atp酶的抑制动力学和Mg: atp依赖的质子电转运。寡霉素在1 μg × mg - 1 prot下阻断线粒体atp酶(pH 8.5)和atp合成酶1 (pH 6.6)的磷酸化水解活性。这些浓度的寡霉素不影响Mg: atp依赖的质子运输和Mg: atp依赖的微粒体囊泡跨膜电位差(PD)的超极化。然而，当低霉素浓度高于10 μg × mg−1 prot时，药物的抑制作用变得明显，并且随着低霉素浓度的增加而增强，至少达到240 μg × mg−1 prot。钒酸盐和NO3−敏感的Mg: atp依赖性电致质子转运系统对寡霉素的敏感性相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Plant Science Letters

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