Phycologia最新文献

{"title":"Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition) <b>Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition)</b> . Mona Hoppenrath, Nicolas Chomérat, Takeo Horiguchi, Shauna A. Murray &amp; Lesley Rhodes2023. 376 pp. Senckenberg Gesellschaft für Naturforschung, Frankfurt am Main, Germany. [Senckenberg Buch vol. 88.] 34.90€ [hardback: ISBN 9783510614240].","authors":"Albert Reñé","doi":"10.1080/00318884.2023.2271359","DOIUrl":"https://doi.org/10.1080/00318884.2023.2271359","url":null,"abstract":"\"Marine benthic dinoflagellates – their relevance for science and society (2nd, completely revised edition).\" Phycologia, ahead-of-print(ahead-of-print), p. 1","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting patterns of genetic diversity and population discontinuity in the common red seaweed <i>Gracilaria salicornia</i> (Gracilariaceae) along the coasts of Thailand","authors":"Narongrit Muangmai, Sinchai Maneekat, Anong Chirapart, Giuseppe C. Zuccarello","doi":"10.1080/00318884.2023.2254621","DOIUrl":"https://doi.org/10.1080/00318884.2023.2254621","url":null,"abstract":"ABSTRACTFor long-term management and conservation of marine resources an understanding of their genetic diversity and geographic distribution is necessary. The red alga Gracilaria salicornia is widely distributed along the coasts of Thailand and has been used as food and as fertilizer. However, there have been no studies examining the genetic diversity of this alga. The present research aimed to employ two different genetic markers, mitochondrial (COI-5P) and chloroplast (RuBisCO spacer) for exploring the genetic diversity and phylogeography of G. salicornia in Thailand. Our analyses, inferred from both markers, revealed different levels of genetic diversity between the two coasts of Thailand, and the recognition of seven new COI haplotypes. Gracilaria salicornia in the Andaman Sea had higher genetic diversity than in the Gulf of Thailand. Significant genetic divergence was found between populations in the Andaman Sea and the Gulf of Thailand, likely due to the geographic barrier of the Thai-Malay Peninsula and the complex ocean currents. No genetic structuring was detected in the Gulf of Thailand, whereas low to moderate genetic differentiation was found between populations in the Andaman Sea, suggesting a possible break between the northern and southern Andaman Sea. This break could be explained by ocean circulation patterns around the Malacca Strait. These findings provide further evidence supporting the Thai-Malay Peninsula and oceanographic currents as strong barriers to dispersal, leading to genetic differentiation and population structuring of G. salicornia and other marine species. These results can be used to guide conservation management actions and exploitation of this marine resource.KEYWORDS: COIMacroalgaePopulation geneticsPhylogeographyRhodophyta ACKNOWLEDGEMENTSWe thank Jantana Praiboon and Jakaphan Bulan for help collecting and sequencing samples. We also thank the associate editor Gavin Maneveldt and two anonymous reviewers for their comments and suggestions.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2254621Additional informationFundingThis work was supported by a research grant to NM from the Thailand Research Fund (MRG6080040).","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light irradiance modifies the fatty acid composition of <i>Amphidinium carterae</i> (Dinophyceae)","authors":"Armando Mendoza-Flores, M. del Pilar Sánchez-Saavedra","doi":"10.1080/00318884.2023.2244813","DOIUrl":"https://doi.org/10.1080/00318884.2023.2244813","url":null,"abstract":"ABSTRACTAmphidinium carterae is a dinoflagellate of increasing interest for producing bioactive compounds. The aim of this study was to investigate the effect of five irradiances (50, 100, 150, 200 and 250 µmol photon m–2 s–1) on the fatty acid profile of A. carterae. The results show that high irradiances increase the lipid productivity (36.65 mg l–1 d–1 at 50 µmol photon m–2 s–1 to 90 mg l–1 d–1 at 250 µmol photon m–2 s–1). High irradiance increased the content of polyunsaturated fatty acids (PUFAs) relative to the total content of fatty acids (40.0% at 50 µmol photon m–2 s–1 to 53.23% at 250 µmol photon m–2 s–1) and decreased the amount of monounsaturated fatty acids (MUFAs) (14.30% at 50 µmol photon m–2 s–1 to 6.04% at 250 µmol photon m–2 s–1). The higher PUFAs content was due to docosahexaenoic acid (DHA; 20.76% at 50 µmol photon m–2 s–1 to 30.63% at 250 µmol photon m–2 s–1) and eicosapentaenoic acid (EPA; 14.91% at 50 µmol photon m–2 s–1 to 18.96% at 250 µmol photon m–2 s–1). Palmitic acid (C16:0) had the highest content among saturated fatty acids (19.87% at 50 µmol photon m–2 s–1 to 22.29% at 250 µmol photon m–2 s–1). The proportion of neutral lipids tended to increase under high irradiances (100–250 µmol photon m–2 s–1). At the lowest irradiance (50 µmol photon m–2 s–1), the proportion of polar lipids was higher than that of neutral lipids. PUFAs had the main content within polar lipids at the five irradiances (47%–50%). The major neutral lipids were SFAs at the five irradiances (47%–51%). It was concluded that the irradiance levels influence the lipid metabolism of A. carterae. The increase in irradiance level raises the production of lipids and the content of EPA and DHA for potential utilization in the biotechnological, aquaculture and nutraceutical industries.KEYWORDS: DinoflagellatesDocosahexaenoic acidLipid classesPolyunsaturated fatty acids AUTHOR CONTRIBUTIONSA.M.F.: design and implementation of the experiments and manuscript preparation.M.P.S.S. supervision of experiments and data processing, contribution to manuscript preparation, and financial support.STATEMENT OF INFORMED CONSENT, HUMAN/ANIMAL RIGHTSNo conflicts, informed consent, or human or animal rights are applicable to this study.ACKNOWLEDGEMENTSThis work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant Project: SEP-CONACyT 130074), and CICESE (Grant Project: 623108). A.M.F. thanks the postdoctoral scholarship from the Oceanography Division of CICESE and the postdoctoral scholarship from CONACyT.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California [623108].","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Endoplura gyeokpoensis sp. nov</i> . and <i>E. limpeticola sp. nov</i> . (Ralfsiales, Phaeophyceae), two new crustose brown algae from Korea","authors":"Antony Otinga Oteng’o, Boo Yeon Won, Tae Oh Cho","doi":"10.1080/00318884.2023.2247713","DOIUrl":"https://doi.org/10.1080/00318884.2023.2247713","url":null,"abstract":"ABSTRACTEndoplura gyeokpoensis sp. nov. and E. limpeticola sp. nov. from Korea are described based on molecular and morphological analyses. The crustose brown algal genus Endoplura previously comprised three species: E. aurea, E. jejuensis and E. koreana. Our phylogenetic analyses inferred from rbcL and concatenated rbcL and COI-5P data revealed that E. gyeokpoensis sp. nov. and E. limpeticola sp. nov. are placed in the Endoplura clade with robust bootstrap support and Bayesian posterior probabilities. Endoplura gyeokpoensis is characterized by small crustose thalli, thallus outline with a lighter margin, and 2–4 plurangia terminated by 2–3 sterile cells. Endoplura limpeticola is distinguished by epizoic crustose thalli on limpets, thick thalli up to 751 μm, thallus outline with a darker margin, multicellular rhizoids, and two plurangia terminated by 2–3 sterile cells. Our study shows that the two new Endoplura species are molecularly and morphologically distinguished from each other as well as from the congeners.KEYWORDS: COI-5PEpizoicMorphologyPhylogenyrbcLTaxonomy ACKNOWLEDGEMENTSWe are grateful to Dr. Shinya Uwai for his valuable comments on the article and to two anonymous reviewers for improving the manuscript.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2247713Additional informationFundingThis study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A2059577), by the Ministry of Ocean and Fisheries (Marine Biotics Project, 20210469), and by the National Marine Biodiversity Institute of Korea (the management of Marine Fishery Bio-resources Center 2023) to Tae Oh Cho. This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01051909) to Boo Yeon Won.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic revision of the <i>Grateloupia</i> complex (Rhodophyta) from Vietnam using <i>rbc</i> L and LSU sequences, with the description of <i>Phyllymenia nhatrangensis sp. nov.</i>","authors":"Xuan-Vy Nguyen, Lawrence M. Liao, Showe-Mei Lin","doi":"10.1080/00318884.2023.2247175","DOIUrl":"https://doi.org/10.1080/00318884.2023.2247175","url":null,"abstract":"ABSTRACTGrateloupia sensu lato has had a long and troublesome taxonomic history because of superficial similarities in morphology, ill-defined species boundaries and lack of diagnostic characters that leave many gaps that still need to be fully resolved. In this study, we carried out detailed observations on female reproductive structures, and inferred the phylogenetic relationships of the Grateloupia-like species occurring in Vietnam based on rbcL and LSU rDNA sequence analyses. The new species Phyllymenia nhatrangensis sp. nov. differs from its sister species P. gibbesii in the thickness of the cortex. The new species is slightly flattened, composed of two to four main blades, bearing numerous marginal and a few superficial, lanceolate bladelets. Blade sizes of P. nhatrangensis are also smaller than those of P. gibbesii, which are longer and wider. However, the new species shares with species from western Pacific Ocean, such as P. taiwanensis and P. huangiae, the presence of frequent marginal proliferations. Based on rbcL, the divergence between P. nhatrangensis and P. gibbesii was 3.3% (39 bp). With the present addition, Grateloupia sensu lato now comprises 16 species, including nine species of Grateloupia, three species of Phyllymenia, two species of Yonagunia and one species each of Dermocorynus and Prionitis.KEYWORDS: Auxiliary cell ampullaeMorphologyPhylogenetic analysis ACKNOWLEDGEMENTSWe thank Dr. Dao Viet Ha, director of the Institute of Oceanography, the staff of its Department of Marine Botany, and the Vietnam Academy of Science and Technology Key Lab on Food and Environmental Safety (Central Vietnam), Institute of Oceanography, Vietnam for the permission to use their equipment. LML acknowledges the travel support granted by the Graduate School of Integrated Sciences for Life, Hiroshima University. The critical suggestions from two anonymous reviewers have helped improve the manuscript and are gratefully acknowledged.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2247175Additional informationFundingThis work was supported by Vietnam Academy of Science and Technology, under Grant TĐĐTB0.04/21-23.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomy and phylogeny of <i>Chaetoceros</i> species of the section <i>Stenocincta</i> (Bacillariophyceae), with emendation of <i>C. affinis</i> and <i>C. willei</i> and description of three new species","authors":"Xudan Lu, Zuoyi Chen, David U. Hernández-Becerril, Nina Lundholm, Yang Li","doi":"10.1080/00318884.2023.2257128","DOIUrl":"https://doi.org/10.1080/00318884.2023.2257128","url":null,"abstract":"ABSTRACTSpecies of Chaetoceros sect. Stenocincta are frequently observed in marine phytoplankton. To study species diversity within the section Stenocincta, monoclonal strains were established from Chinese coastal waters and Norwegian Sea. Following detailed morphological observations and molecular evidence inferred from nucleus-encoded large subunit ribosomal DNA, the descriptions of C. affinis and C. willei are emended based on isolates from their respective type localities. Additionally, three new species are described. Chaetoceros donghaiensis sp. nov. is characterized by short straight chains, with thin setae only, and terminal setae that tend to cross distally. Chaetoceros forceps sp. nov. is unique by its thick terminal setae, each with an inflation near the end, with each pair forming a V-shape, resembling forceps. Chaetoceros lepidus sp. nov. is characterized by short, straight chains, narrowly elliptical valve faces, setae all located in the apical plane, and arched intercalary setae in opposite directions to the chain axis in broad girdle views. Three other distinct clades, which may also represent new species, were discerned in the LSU rDNA phylogeny, but more morphological characters are needed before formal proposals can be made. These species/clades formed a well-supported monophyletic lineage, supporting the current circumscription of section Stenocincta. This section is characterized by colonies in straight chains, cells with one chloroplast only, narrow lanceolate apertures, and terminal setae generally thicker than intercalary ones or both similar and thin. However, in C. diversus the terminal setae are thin and distinctly thicker pairs of intercalary setae are usual.KEYWORDS: MorphologyLSU rDNAChaetoceros affinisChaetoceros willeiChaetoceros donghaiensisChaetoceros forcepsChaetoceros lepidus DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the National Nature Science Foundation of China [32170206]; Joint Fund of National Science Foundation of China and Chinese Shandong Province [U2106205]; National Key Research and Development Program of China [2022YFC3105201].","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the diversity of the genus <i>Ulva</i> (Ulvales, Chlorophyta) in southeastern Brazil, with a description of <i>Ulva kanagawae sp. nov.</i>","authors":"Victor Andrei Rodrigues Carneiro, Nuno Tavares Martins, Sebastiana Lidielda Albuquerque da Silva, Maria Beatriz de Barros-Barreto, Sonia Barreto Pereira, Valéria Cassano","doi":"10.1080/00318884.2023.2243433","DOIUrl":"https://doi.org/10.1080/00318884.2023.2243433","url":null,"abstract":"ABSTRACTThe green algal genus Ulva is one of the most widely distributed macroalgal genera. The taxonomy of Ulva is problematic due to its simple morphology. The study of the diversity of this genus has undergone great advances using molecular data, leading to changes in the taxonomic status of species, and the recognition of species complexes and cryptic species. Here we reassessed Ulva species from southeastern Brazil using molecular data. We recognized 10 taxa, among which only three previously reported species were confirmed by molecular data: U. compressa, U. lactuca and U. ohnoi, the latter recently recorded from insular waters in Fernando de Noronha Archipelago, northeastern Brazil. Our phylogenetic analyses and species delimitation methods strongly supported the establishment of Ulva kanagawae sp. nov. The species traditionally cited for southeastern Brazil, U. flexuosa, U. linza, U. rigida and U. paradoxa, proved to be misapplied names of U. tepida, U. aragoënsis, U. ohnoi/U. lactuca and U. torta, respectively. One taxon, Ulva sp., remains unnamed and needs further studies. Ulva chaugulei and U. tanneri, both considered here as cryptogenic species, are new occurrences for Brazil expanding their geographic distribution to the Atlantic Ocean and western Atlantic Ocean, respectively. In addition, our analysis of sequences from type materials revealed that U. pseudo-ohnoi is a heterotypic synonym of U. conglobata. This first systematic molecular study of Ulva species in Brazil points out that more extensive sampling is needed to reveal the true diversity of the genus in Brazilian waters.KEYWORDS: PhylogenyrbcLtaxonomytufA ACKNOWLEDGEMENTSWe thank Joel Campos De-Paula, Renan Oliveira, Érico Atílio Teles, Mutue Toyota Fujii, Cristina Aparecida Nassar, Mateus Henrique Oliveira Pinto, Luanda Soares, Fábio Nauer, Maria Irisvalda L. G. Cavalcanti, Helena R. Fragoso, Bruno Sandy and Souto Neto for field assistance.DISCLOSURE STATEMENTThere are no conflicts of interest to be declared by the authors.Supplementary InformationSupplemental data for this article can be accessed online at https://doi.org/10.1080/00318884.2023.2243433Additional informationFundingThis work was supported by São Paulo Research Foundation (FAPESP, 2018/06085-1) to VC, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Productivity Fellowship (proc. 304141/2020-8) to VC. This study was financed in part by the Rio de Janeiro Research Foundation (Biota FAPERJ, E-26/110.019/2011) to MBB-B and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001 (scholarships to VARC, NTM and SLAS).","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134949726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asia Pacific Sporolithon (Corallinophycidae, Rhodophyta) species revised based on DNA sequencing of type specimens and including S. crypticum sp. nov., S. immotum sp. nov. and S. nodosum sp. nov.","authors":"P. Gabrielson, Jeffery R. Hughey, V. Peña, J. Richards, G. Saunders, B. Twist, T. Farr, W. Nelson","doi":"10.1080/00318884.2023.2225309","DOIUrl":"https://doi.org/10.1080/00318884.2023.2225309","url":null,"abstract":"ABSTRACT To address the taxonomic uncertainty of Sporolithon species named in the early to mid-20th century, targeted PCR sequencing was performed on eight historical type specimens and on recently collected specimens. Six type specimens amplified for the rbcL gene and were Sanger sequenced yielding sequences ranging in length from 118 to 280 base pairs (bp). One, S. australasicum, failed to amplify and another, S. howei, was amplified for the psbA gene yielding a sequence 544 bp in length. The 118 bp long rbcL sequence of the lectotype of S. crassiramosum showed that it is a later, heterotypic synonym of S. molle. The rbcL sequences of type specimens of S. episoredion, S. schmidtii, S. sibogae and S. timorense ranged from 118 to 228 bp, and each is a distinct species. The 544 bp long psbA sequence of S. howei is also unique. The 280 bp long rbcL sequence of the lectotype of S. durum did not match any sequence with that name in any public repository, including the previously published complete plastome and mitogenome sequences. However, it was identical in sequence to a specimen in GenBank from the southern coast of Western Australia as well as several other sequences generated from field-collected specimens from the states of South Australia and Western Australia. The rhodolith specimens from New Zealand previously called S. durum are S. nodosum sp. nov. The species is endemic to New Zealand. The epilithic specimens from New Zealand previously called S. durum are S. immotum sp. nov., which is also found along the southeastern coast of Australia. Sporolithon crypticum sp. nov. is described from the southern coast of Western Australia. RAxML and Bayesian phylogenetic analyses of Sporolithon psbA and rbcL sequences are congruent between the two plastid encoded genes. DNA sequencing of type specimens of species of corallines is demonstrated to be the only reliable method to correctly apply names.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46745492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, molecular and toxinological analyses of Alexandrium affine (Dinophyceae) from marine coastal waters of Buenos Aires Province (Argentina)","authors":"J. A. Tardivo Kubis, F. Rodríguez, A. Rossignoli, P. Riobó, Delfina Aguiar Juárez, E. Sar, Inés Sunesen","doi":"10.1080/00318884.2023.2223796","DOIUrl":"https://doi.org/10.1080/00318884.2023.2223796","url":null,"abstract":"ABSTRACT In the framework of a monitoring program of harmful microalgae from the marine coastal waters of Buenos Aires Province to mitigate the impacts of harmful algal blooms on the aquaculture and marine life and to protect human health, a strain of Alexandrium affine (LPCc012) was isolated. Alexandrium affine is a worldwide distributed species reported as harmful algal bloom-forming, with toxin- and non-toxin-producing strains. Field and cultured materials from Buenos Aires Province were examined using light and scanning electron microscopy, morphologically and genetically (LSU rDNA partial sequencing) characterized, and compared with material from different regions worldwide. Toxin analyses using ultra-performance liquid chromatography with fluorescence detection and liquid chromatography-mass spectrometry methods failed to detect any paralytic or lipophilic shellfish toxins. This is the first record of A. affine from Argentina.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"62 1","pages":"341 - 351"},"PeriodicalIF":1.6,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45832836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing genetic variation of Gelidium canariense (Rhodophyta) based on new and historical collections","authors":"B. Alfonso, Olga Ortega-Martínez, José Carlos Hernández, M. Sansón, C. Sangil, Ricardo T. Pereyra","doi":"10.1080/00318884.2023.2242719","DOIUrl":"https://doi.org/10.1080/00318884.2023.2242719","url":null,"abstract":"ABSTRACT Genetic sequencing of herbarium specimens provides invaluable information on species genetic history. However, several factors hinder the extraction of high-quality DNA from long-term preserved specimens. Our goal was to study the genetic variability of the endemic and habitat-forming macroalga Gelidium canariense over the last 40 years using the mitochondrial intergenic marker cox2-3 spacer. We also studied the genetic diversity at the mesoscale (<100 km), i.e. between two localities on the north coast of Tenerife and at the macroscale (>100 km) using specimens collected on the island of La Palma. We found the presence of the same haplotype at the same location for the last 40 years. This haplotype also coincided within populations of Puerto de la Cruz and Garachico (Tenerife Island) and between populations of different islands (Tenerife and La Palma). This study provides a baseline (i.e. extraction method, PCR protocol for cox2-3 spacer molecular marker, level of DNA degradation of herbarium samples) of the genetic characterization of G. canariense that can be used in future molecular studies to better understand the distribution of genetic diversity in this vulnerable species.","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":"62 1","pages":"383 - 390"},"PeriodicalIF":1.6,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44410336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}