光辐照度改变甲藻藻(甲藻科)的脂肪酸组成

IF 1.5 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Phycologia Pub Date : 2023-09-03 DOI:10.1080/00318884.2023.2244813

Armando Mendoza-Flores, M. del Pilar Sánchez-Saavedra

{"title":"光辐照度改变甲藻藻(甲藻科)的脂肪酸组成","authors":"Armando Mendoza-Flores, M. del Pilar Sánchez-Saavedra","doi":"10.1080/00318884.2023.2244813","DOIUrl":null,"url":null,"abstract":"ABSTRACTAmphidinium carterae is a dinoflagellate of increasing interest for producing bioactive compounds. The aim of this study was to investigate the effect of five irradiances (50, 100, 150, 200 and 250 µmol photon m–2 s–1) on the fatty acid profile of A. carterae. The results show that high irradiances increase the lipid productivity (36.65 mg l–1 d–1 at 50 µmol photon m–2 s–1 to 90 mg l–1 d–1 at 250 µmol photon m–2 s–1). High irradiance increased the content of polyunsaturated fatty acids (PUFAs) relative to the total content of fatty acids (40.0% at 50 µmol photon m–2 s–1 to 53.23% at 250 µmol photon m–2 s–1) and decreased the amount of monounsaturated fatty acids (MUFAs) (14.30% at 50 µmol photon m–2 s–1 to 6.04% at 250 µmol photon m–2 s–1). The higher PUFAs content was due to docosahexaenoic acid (DHA; 20.76% at 50 µmol photon m–2 s–1 to 30.63% at 250 µmol photon m–2 s–1) and eicosapentaenoic acid (EPA; 14.91% at 50 µmol photon m–2 s–1 to 18.96% at 250 µmol photon m–2 s–1). Palmitic acid (C16:0) had the highest content among saturated fatty acids (19.87% at 50 µmol photon m–2 s–1 to 22.29% at 250 µmol photon m–2 s–1). The proportion of neutral lipids tended to increase under high irradiances (100–250 µmol photon m–2 s–1). At the lowest irradiance (50 µmol photon m–2 s–1), the proportion of polar lipids was higher than that of neutral lipids. PUFAs had the main content within polar lipids at the five irradiances (47%–50%). The major neutral lipids were SFAs at the five irradiances (47%–51%). It was concluded that the irradiance levels influence the lipid metabolism of A. carterae. The increase in irradiance level raises the production of lipids and the content of EPA and DHA for potential utilization in the biotechnological, aquaculture and nutraceutical industries.KEYWORDS: DinoflagellatesDocosahexaenoic acidLipid classesPolyunsaturated fatty acids AUTHOR CONTRIBUTIONSA.M.F.: design and implementation of the experiments and manuscript preparation.M.P.S.S. supervision of experiments and data processing, contribution to manuscript preparation, and financial support.STATEMENT OF INFORMED CONSENT, HUMAN/ANIMAL RIGHTSNo conflicts, informed consent, or human or animal rights are applicable to this study.ACKNOWLEDGEMENTSThis work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant Project: SEP-CONACyT 130074), and CICESE (Grant Project: 623108). A.M.F. thanks the postdoctoral scholarship from the Oceanography Division of CICESE and the postdoctoral scholarship from CONACyT.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California [623108].","PeriodicalId":20140,"journal":{"name":"Phycologia","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Light irradiance modifies the fatty acid composition of <i>Amphidinium carterae</i> (Dinophyceae)\",\"authors\":\"Armando Mendoza-Flores, M. del Pilar Sánchez-Saavedra\",\"doi\":\"10.1080/00318884.2023.2244813\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACTAmphidinium carterae is a dinoflagellate of increasing interest for producing bioactive compounds. The aim of this study was to investigate the effect of five irradiances (50, 100, 150, 200 and 250 µmol photon m–2 s–1) on the fatty acid profile of A. carterae. The results show that high irradiances increase the lipid productivity (36.65 mg l–1 d–1 at 50 µmol photon m–2 s–1 to 90 mg l–1 d–1 at 250 µmol photon m–2 s–1). High irradiance increased the content of polyunsaturated fatty acids (PUFAs) relative to the total content of fatty acids (40.0% at 50 µmol photon m–2 s–1 to 53.23% at 250 µmol photon m–2 s–1) and decreased the amount of monounsaturated fatty acids (MUFAs) (14.30% at 50 µmol photon m–2 s–1 to 6.04% at 250 µmol photon m–2 s–1). The higher PUFAs content was due to docosahexaenoic acid (DHA; 20.76% at 50 µmol photon m–2 s–1 to 30.63% at 250 µmol photon m–2 s–1) and eicosapentaenoic acid (EPA; 14.91% at 50 µmol photon m–2 s–1 to 18.96% at 250 µmol photon m–2 s–1). Palmitic acid (C16:0) had the highest content among saturated fatty acids (19.87% at 50 µmol photon m–2 s–1 to 22.29% at 250 µmol photon m–2 s–1). The proportion of neutral lipids tended to increase under high irradiances (100–250 µmol photon m–2 s–1). At the lowest irradiance (50 µmol photon m–2 s–1), the proportion of polar lipids was higher than that of neutral lipids. PUFAs had the main content within polar lipids at the five irradiances (47%–50%). The major neutral lipids were SFAs at the five irradiances (47%–51%). It was concluded that the irradiance levels influence the lipid metabolism of A. carterae. The increase in irradiance level raises the production of lipids and the content of EPA and DHA for potential utilization in the biotechnological, aquaculture and nutraceutical industries.KEYWORDS: DinoflagellatesDocosahexaenoic acidLipid classesPolyunsaturated fatty acids AUTHOR CONTRIBUTIONSA.M.F.: design and implementation of the experiments and manuscript preparation.M.P.S.S. supervision of experiments and data processing, contribution to manuscript preparation, and financial support.STATEMENT OF INFORMED CONSENT, HUMAN/ANIMAL RIGHTSNo conflicts, informed consent, or human or animal rights are applicable to this study.ACKNOWLEDGEMENTSThis work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant Project: SEP-CONACyT 130074), and CICESE (Grant Project: 623108). A.M.F. thanks the postdoctoral scholarship from the Oceanography Division of CICESE and the postdoctoral scholarship from CONACyT.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California [623108].\",\"PeriodicalId\":20140,\"journal\":{\"name\":\"Phycologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phycologia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/00318884.2023.2244813\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phycologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/00318884.2023.2244813","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

摘要黄斑双鞭毛藻是一种因生产生物活性化合物而日益受到关注的鞭毛藻。研究了50、100、150、200和250µmol光子m-2 s-1辐照对黄芪脂肪酸谱的影响。结果表明，高辐照度提高了脂质产量(50µmol光子m-2 s-1时为36.65 mg l-1 d-1, 250µmol光子m-2 s-1时为90 mg l-1 d-1)。高辐照增加了多不饱和脂肪酸(PUFAs)相对于脂肪酸总含量的含量(在50µmol光子m-2 s-1下为40.0%，在250µmol光子m-2 s-1下为53.23%)，降低了单不饱和脂肪酸(MUFAs)的含量(在50µmol光子m-2 s-1下为14.30%，在250µmol光子m-2 s-1下为6.04%)。PUFAs含量较高是由于二十二碳六烯酸(DHA);50µmol光子m-2 s-1时为20.76%，250µmol光子m-2 s-1时为30.63%)和二十碳五烯酸(EPA;50µmol光子m-2 s-1时为14.91%，250µmol光子m-2 s-1时为18.96%)。饱和脂肪酸中棕榈酸(C16:0)的含量最高，在50µmol光子m-2 s-1时为19.87%，在250µmol光子m-2 s-1时为22.29%。高辐照度(100-250µmol光子m-2 s-1)下，中性脂的比例有增加的趋势。在最低辐照度(50µmol光子m-2 s-1)下，极性脂质比例高于中性脂质比例。5种辐照度下PUFAs主要含量在极性脂质内(47% ~ 50%)。在5种辐照度(47%-51%)下，主要中性脂质为sfa。综上所述，光照水平影响了黄颡鱼的脂质代谢。辐照水平的增加提高了脂类的产量以及EPA和DHA的含量，可用于生物技术、水产养殖和营养保健工业。关键词:甲藻;二十二碳六烯酸;脂类;多不饱和脂肪酸;监督实验和数据处理，参与稿件准备，并提供资金支持。知情同意、人类/动物权利声明本研究不涉及冲突、知情同意或人类/动物权利。本工作由国家科学委员会Tecnología (CONACyT)(资助项目:SEP-CONACyT 130074)和CICESE(资助项目:623108)资助。A.M.F.感谢中国科学院海洋学部博士后奖学金和中国科学院海洋研究所博士后奖学金。声明作者未报告潜在的利益冲突。本研究得到了Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California的支持[623108]。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Light irradiance modifies the fatty acid composition of Amphidinium carterae (Dinophyceae)

ABSTRACTAmphidinium carterae is a dinoflagellate of increasing interest for producing bioactive compounds. The aim of this study was to investigate the effect of five irradiances (50, 100, 150, 200 and 250 µmol photon m–2 s–1) on the fatty acid profile of A. carterae. The results show that high irradiances increase the lipid productivity (36.65 mg l–1 d–1 at 50 µmol photon m–2 s–1 to 90 mg l–1 d–1 at 250 µmol photon m–2 s–1). High irradiance increased the content of polyunsaturated fatty acids (PUFAs) relative to the total content of fatty acids (40.0% at 50 µmol photon m–2 s–1 to 53.23% at 250 µmol photon m–2 s–1) and decreased the amount of monounsaturated fatty acids (MUFAs) (14.30% at 50 µmol photon m–2 s–1 to 6.04% at 250 µmol photon m–2 s–1). The higher PUFAs content was due to docosahexaenoic acid (DHA; 20.76% at 50 µmol photon m–2 s–1 to 30.63% at 250 µmol photon m–2 s–1) and eicosapentaenoic acid (EPA; 14.91% at 50 µmol photon m–2 s–1 to 18.96% at 250 µmol photon m–2 s–1). Palmitic acid (C16:0) had the highest content among saturated fatty acids (19.87% at 50 µmol photon m–2 s–1 to 22.29% at 250 µmol photon m–2 s–1). The proportion of neutral lipids tended to increase under high irradiances (100–250 µmol photon m–2 s–1). At the lowest irradiance (50 µmol photon m–2 s–1), the proportion of polar lipids was higher than that of neutral lipids. PUFAs had the main content within polar lipids at the five irradiances (47%–50%). The major neutral lipids were SFAs at the five irradiances (47%–51%). It was concluded that the irradiance levels influence the lipid metabolism of A. carterae. The increase in irradiance level raises the production of lipids and the content of EPA and DHA for potential utilization in the biotechnological, aquaculture and nutraceutical industries.KEYWORDS: DinoflagellatesDocosahexaenoic acidLipid classesPolyunsaturated fatty acids AUTHOR CONTRIBUTIONSA.M.F.: design and implementation of the experiments and manuscript preparation.M.P.S.S. supervision of experiments and data processing, contribution to manuscript preparation, and financial support.STATEMENT OF INFORMED CONSENT, HUMAN/ANIMAL RIGHTSNo conflicts, informed consent, or human or animal rights are applicable to this study.ACKNOWLEDGEMENTSThis work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) (Grant Project: SEP-CONACyT 130074), and CICESE (Grant Project: 623108). A.M.F. thanks the postdoctoral scholarship from the Oceanography Division of CICESE and the postdoctoral scholarship from CONACyT.DISCLOSURE STATEMENTNo potential conflict of interest was reported by the authors.Additional informationFundingThis work was supported by the Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California [623108].

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Phycologia 生物-海洋与淡水生物学

CiteScore

3.10

自引率

18.80%

发文量

审稿时长

3 months

期刊介绍： Phycologia is published bimonthly by the International Phycological Society and serves as a publishing medium for information about any aspect of phycology. Membership in the Society is not necessary for publication. Submitted manuscripts cannot be previously published or submitted elsewhere. Copyright ownership of all accepted papers is held by the International Phycological Society.