基于新和历史资料的加那利胶的遗传变异追踪

IF 1.5 4区 生物学 Q3 MARINE & FRESHWATER BIOLOGY
B. Alfonso, Olga Ortega-Martínez, José Carlos Hernández, M. Sansón, C. Sangil, Ricardo T. Pereyra
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引用次数: 0

摘要

植物标本馆标本的基因测序为物种遗传史提供了宝贵的信息。然而,有几个因素阻碍了从长期保存的标本中提取高质量的DNA。本研究的目的是利用线粒体基因间标记cox2-3间隔物研究加那利胶藻(Gelidium canariense)近40年来的遗传变异。我们还利用在拉帕尔马岛采集的样本,在中尺度(100公里)研究了遗传多样性。在过去的40年里,我们在同一地点发现了相同的单倍型。这种单倍型在Puerto de la Cruz和Garachico(特内里费岛)的人群中以及不同岛屿(特内里费岛和拉帕尔马岛)的人群中也有重合。本研究为金丝雀遗传特征提供了一个基线(即提取方法、cox2-3间隔分子标记PCR方案、标本DNA降解水平),可用于未来的分子研究,以更好地了解这一脆弱物种的遗传多样性分布。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tracing genetic variation of Gelidium canariense (Rhodophyta) based on new and historical collections
ABSTRACT Genetic sequencing of herbarium specimens provides invaluable information on species genetic history. However, several factors hinder the extraction of high-quality DNA from long-term preserved specimens. Our goal was to study the genetic variability of the endemic and habitat-forming macroalga Gelidium canariense over the last 40 years using the mitochondrial intergenic marker cox2-3 spacer. We also studied the genetic diversity at the mesoscale (<100 km), i.e. between two localities on the north coast of Tenerife and at the macroscale (>100 km) using specimens collected on the island of La Palma. We found the presence of the same haplotype at the same location for the last 40 years. This haplotype also coincided within populations of Puerto de la Cruz and Garachico (Tenerife Island) and between populations of different islands (Tenerife and La Palma). This study provides a baseline (i.e. extraction method, PCR protocol for cox2-3 spacer molecular marker, level of DNA degradation of herbarium samples) of the genetic characterization of G. canariense that can be used in future molecular studies to better understand the distribution of genetic diversity in this vulnerable species.
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来源期刊
Phycologia
Phycologia 生物-海洋与淡水生物学
CiteScore
3.10
自引率
18.80%
发文量
54
审稿时长
3 months
期刊介绍: Phycologia is published bimonthly by the International Phycological Society and serves as a publishing medium for information about any aspect of phycology. Membership in the Society is not necessary for publication. Submitted manuscripts cannot be previously published or submitted elsewhere. Copyright ownership of all accepted papers is held by the International Phycological Society.
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