Photosynthesis Research最新文献_第8页

Peculiarities of DNP-INT and DBMIB as inhibitors of the photosynthetic electron transport DNP-INT 和 DBMIB 作为光合电子传递抑制剂的特殊性

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-12-18 DOI: 10.1007/s11120-023-01063-5

{"title":"Peculiarities of DNP-INT and DBMIB as inhibitors of the photosynthetic electron transport","authors":"","doi":"10.1007/s11120-023-01063-5","DOIUrl":"https://doi.org/10.1007/s11120-023-01063-5","url":null,"abstract":"<h3>Abstract</h3> Inhibitory analysis is a useful tool for studying cytochrome b6f complex in the photosynthetic electron transport chain. Here, we examine the inhibitory efficiency of two widely used inhibitors of the plastoquinol oxidation in the cytochrome b6f complex, namely 2,4-dinitrophenyl ether of 2-iodo-4-nitrothymol (DNP-INT) and 2,5-dibromo-3-methyl-6-isopropylbenzoquinone (DBMIB). Using isolated thylakoids from pea and arabidopsis, we demonstrate that inhibitory activity of DNP-INT and DBMIB is enhanced by increasing irradiance, and this effect is due to the increase in the rate of electron transport. However, the accumulation of protons in the thylakoid lumen at low light intensity has opposite effects on the inhibitory activity of DNP-INT and DBMIB, namely increasing the activity of DNP-INT and restricting the activity of DBMIB. These results allow for the refinement of the conditions under which the use of these inhibitors leads to the complete inhibition of plastoquinol oxidation in the cytochrome b6f complex, thereby broadening our understanding of the operation of the cytochrome b6f complex under conditions of steady-state electron transport.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138717156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the significance of chlorophyll b in the moss Physcomitrium patens by knocking out two functional chlorophyllide a oxygenase. 通过敲除两个功能性叶绿素a加氧酶揭示叶绿素b在苔藓中的意义。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-12-01 Epub Date: 2023-08-31 DOI: 10.1007/s11120-023-01044-8

Lin Zhang, Chunhong Yang, Cheng Liu

引用次数: 0

Correction to: Derivative-based time-adjusted analysis of diurnal and within-tree variation in the OJIP fluorescence transient of silver birch. 修正：基于导数的时间调整分析，对白桦OJIP荧光瞬态的昼夜变化和树内变化进行分析。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-12-01 DOI: 10.1007/s11120-023-01050-w

Olusegun Olaitan Akinyemi, Jaroslav Čepl, Sarita Keski-Saari, Ivana Tomášková, Jan Stejskal, Sari Kontunen-Soppela, Markku Keinänen

引用次数: 0

The slope of assimilation rate against stomatal conductance should not be used as a measure of water use efficiency or stomatal control over assimilation. 同化率相对于气孔导度的斜率不应被用作衡量水分利用效率或气孔对同化的控制。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-12-01 Epub Date: 2023-10-30 DOI: 10.1007/s11120-023-01054-6

Chandra Bellasio

引用次数: 0

Photosynthetic performance under adaxial and abaxial illumination in three Mediterranean Quercus species differing in branch architecture and individual leaf area. 不同枝型和单叶面积的三种地中海栎在正面和背面光照下的光合性能

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-12-01 Epub Date: 2023-08-31 DOI: 10.1007/s11120-023-01045-7

Sonia Mediavilla, Alfonso Escudero

{"title":"Photosynthetic performance under adaxial and abaxial illumination in three Mediterranean Quercus species differing in branch architecture and individual leaf area.","authors":"Sonia Mediavilla, Alfonso Escudero","doi":"10.1007/s11120-023-01045-7","DOIUrl":"10.1007/s11120-023-01045-7","url":null,"abstract":"Light availability effects on canopy-level carbon balance constitute an especially difficult issue to address, owing to the strong spatial and temporal changes of the light environment within the canopy. One of the least explored aspects in relation to light environment is the interaction between leaf angle and leaf anatomy. The inclination of the leaf may affect the distribution of light between the adaxial and abaxial surface. The purpose of this study is determining the proportions of the leaf area receiving light from the abaxial side in branches of isolated trees in three Mediterranean oaks, as well as the photosynthetic responses to light under adaxial and abaxial illumination. The proportions of the leaf area illuminated from below were low for sun incidence angles near 0° with respect to the main axis of the branch. However, for sun incidence angles about 45°, the proportion of leaves receiving abaxial illumination was considerable. PPFD levels on the sunlit part of the abaxial surface were always lower than those in the upper leaf side, as a consequence of the lower projection efficiency for the leaves facing the sun by the lower side. Light absorptance was also lower on the abaxial side. The differences between both sides of the leaf tended to be stronger for thicker, longer-living leaves. We conclude that mean C assimilation of the canopy is significantly decreased by the presence of leaves facing the sun by the lower side and that this decrease is stronger in evergreen species with thicker leaves.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10477467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The carbon-concentrating mechanism of the extremophilic red microalga Cyanidioschyzon merolae. 更正：极端嗜红微藻Cyanidoschyzon merolae的碳富集机制。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-12-01 DOI: 10.1007/s11120-023-01055-5

Anne K Steensma, Yair Shachar-Hill, Berkley J Walker

引用次数: 0

Tracking the first electron transfer step at the donor side of oxygen-evolving photosystem II by time-resolved infrared spectroscopy. 用时间分辨红外光谱法追踪出氧光系统II供体侧的第一个电子转移步骤。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-11-23 DOI: 10.1007/s11120-023-01057-3

Mohamad Yahia Dekmak, Sarah M Mäusle, Janosch Brandhorst, Philipp S Simon, Holger Dau

{"title":"Tracking the first electron transfer step at the donor side of oxygen-evolving photosystem II by time-resolved infrared spectroscopy.","authors":"Mohamad Yahia Dekmak, Sarah M Mäusle, Janosch Brandhorst, Philipp S Simon, Holger Dau","doi":"10.1007/s11120-023-01057-3","DOIUrl":"https://doi.org/10.1007/s11120-023-01057-3","url":null,"abstract":"In oxygen-evolving photosystem II (PSII), the multi-phasic electron transfer from a redox-active tyrosine residue (TyrZ) to a chlorophyll cation radical (P680+) precedes the water-oxidation chemistry of the S-state cycle of the Mn4Ca cluster. Here we investigate these early events, observable within about 10 ns to 10 ms after laser-flash excitation, by time-resolved single-frequency infrared (IR) spectroscopy in the spectral range of 1310-1890 cm-1 for oxygen-evolving PSII membrane particles from spinach. Comparing the IR difference spectra at 80 ns, 500 ns, and 10 µs allowed for the identification of quinone, P680 and TyrZ contributions. A broad electronic absorption band assignable P680+ was used to trace largely specifically the P680+ reduction kinetics. The experimental time resolution was taken into account in least-square fits of P680+ transients with a sum of four exponentials, revealing two nanosecond phases (30-46 ns and 690-1110 ns) and two microsecond phases (4.5-8.3 µs and 42 µs), which mostly exhibit a clear S-state dependence, in agreement with results obtained by other methods. Our investigation paves the road for further insight in the early events associated with TyrZ oxidation and their role in the preparing the PSII donor side for the subsequent water oxidation chemistry.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138295768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives of cyanobacterial cell factories. 蓝藻细胞工厂的前景。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-11-15 DOI: 10.1007/s11120-023-01056-4

Anastasios Melis, Diego Alberto Hidalgo Martinez, Nico Betterle

引用次数: 0

Theoretical elucidation of the structure, bonding, and reactivity of the CaMn₄O_x clusters in the whole Kok cycle for water oxidation embedded in the oxygen evolving center of photosystem II. New molecular and quantum insights into the mechanism of the O-O bond formation. CaMn4Ox团簇在整个Kok循环中的结构、键合和反应性的理论阐明，用于嵌入光系统II的析氧中心的水氧化。对O-O键形成机制的新的分子和量子见解。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-11-09 DOI: 10.1007/s11120-023-01053-7

Kizashi Yamaguchi, Koichi Miyagawa, Mitsuo Shoji, Takashi Kawakami, Hiroshi Isobe, Shusuke Yamanaka, Takahito Nakajima

{"title":"Theoretical elucidation of the structure, bonding, and reactivity of the CaMn4Ox clusters in the whole Kok cycle for water oxidation embedded in the oxygen evolving center of photosystem II. New molecular and quantum insights into the mechanism of the O-O bond formation.","authors":"Kizashi Yamaguchi, Koichi Miyagawa, Mitsuo Shoji, Takashi Kawakami, Hiroshi Isobe, Shusuke Yamanaka, Takahito Nakajima","doi":"10.1007/s11120-023-01053-7","DOIUrl":"https://doi.org/10.1007/s11120-023-01053-7","url":null,"abstract":"This paper reviews our historical developments of broken-symmetry (BS) and beyond BS methods that are applicable for theoretical investigations of metalloenzymes such as OEC in PSII. The BS hybrid DFT (HDFT) calculations starting from high-resolution (HR) XRD structure in the most stable S1 state have been performed to elucidate structure and bonding of whole possible intermediates of the CaMn4Ox cluster (1) in the Si (i = 0 ~ 4) states of the Kok cycle. The large-scale HDFT/MM computations starting from HR XRD have been performed to elucidate biomolecular system structures which are crucial for examination of possible water inlet and proton release pathways for water oxidation in OEC of PSII. DLPNO CCSD(T0) computations have been performed for elucidation of scope and reliability of relative energies among the intermediates by HDFT. These computations combined with EXAFS, XRD, XFEL, and EPR experimental results have elucidated the structure, bonding, and reactivity of the key intermediates, which are indispensable for understanding and explanation of the mechanism of water oxidation in OEC of PSII. Interplay between theory and experiments have elucidated important roles of four degrees of freedom, spin, charge, orbital, and nuclear motion for understanding and explanation of the chemical reactivity of 1 embedded in protein matrix, indicating the participations of the Ca(H2O)n ion and tyrosine(Yz)-O radical as a one-electron acceptor for the O-O bond formation. The Ca-assisted Yz-coupled O-O bond formation mechanisms for water oxidation are consistent with recent XES and very recent time-resolved SFX XFEL and FTIR results.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic strategies to improve the thermotolerance of photosynthesis. 提高光合作用耐热性的转基因策略。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2023-11-01 Epub Date: 2023-06-05 DOI: 10.1007/s11120-023-01024-y

Amanda P Cavanagh, Donald R Ort

{"title":"Transgenic strategies to improve the thermotolerance of photosynthesis.","authors":"Amanda P Cavanagh, Donald R Ort","doi":"10.1007/s11120-023-01024-y","DOIUrl":"10.1007/s11120-023-01024-y","url":null,"abstract":"Warming driven by the accumulation of greenhouse gases in the atmosphere is irreversible over at least the next century, unless practical technologies are rapidly developed and deployed at scale to remove and sequester carbon dioxide from the atmosphere. Accepting this reality highlights the central importance for crop agriculture to develop adaptation strategies for a warmer future. While nearly all processes in plants are impacted by above optimum temperatures, the impact of heat stress on photosynthetic processes stand out for their centrality. Here, we review transgenic strategies that show promise in improving the high-temperature tolerance of specific subprocesses of photosynthesis and in some cases have already been shown in proof of concept in field experiments to protect yield from high temperature-induced losses. We also highlight other manipulations to photosynthetic processes for which full proof of concept is still lacking but we contend warrant further attention. Warming that has already occurred over the past several decades has had detrimental impacts on crop production in many parts of the world. Declining productivity presages a rapidly developing global crisis in food security particularly in low income countries. Transgenic manipulation of photosynthesis to engineer greater high-temperature resilience holds encouraging promise to help meet this challenge.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9574266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1