Photosynthesis Research最新文献

{"title":"Biochemical and phylogenetic analyses of light-harvesting complexes from Tetraselmis striata.","authors":"Yuma N Yamamoto, Takehiro Suzuki, Yoshifumi Ueno, Tatsuya Tomo, Naoshi Dohmae, Atsushi Takabayashi, Ryo Nagao","doi":"10.1007/s11120-025-01152-7","DOIUrl":"https://doi.org/10.1007/s11120-025-01152-7","url":null,"abstract":"<p><p>Oxygenic photosynthetic organisms employ light-harvesting complexes (LHCs) to capture solar energy and regulate excess excitation. Tetraselmis species belong to Chlorodendrophyceae, one of the earliest-diverging lineages within core Chlorophyta. While these organisms exhibit distinctive pigment compositions, their LHC organization and function remain largely uncharacterized. Here, we examined the biochemical and spectral properties of LHC, PSI-LHCI, and PSII-LHCII complexes from Tetraselmis striata NIES-1019. Pigment analysis identified loroxanthin derivatives, loroxanthin decenoate and loroxanthin dodecenoate, in all three complexes. Notably, these carotenoids are absent in Chlamydomonas reinhardtii and Ostreococcus tauri, implying a lineage-specific adaptation. Fluorescence spectra of PSII-LHCII and PSI-LHCI from T. striata exhibited distinct characteristics compared with their counterparts in C. reinhardtii and land plants, indicating differences in pigment organization. In contrast, LHC fluorescence properties closely resembled those of green-lineage organisms, suggesting conservation of chlorophyll-binding arrangements. Phylogenetic analyses revealed that T. striata possesses LHCBM-based LHCII trimers, consistent with other core Chlorophyta, but its PSI antenna composition diverges from that of these algae. Among LHCIs in the PSI outer belt, only LHCA5a was identified, whereas LHCA4a and LHCA6a were absent, implying structural divergence from C. reinhardtii. These findings provide insights into the evolution of LHCs in Chlorophyta and the distinct pigment-protein interactions underlying Tetraselmis light-harvesting strategies.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"32"},"PeriodicalIF":2.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonpigmented PsbR is involved in the integrity of excitation landscape in higher plant photosystem II, a case study in Arabidopsis thaliana and a mutant.","authors":"Dariusz M Niedzwiedzki, Rupal Singh Tomar, Nikki Cecil M Magdaong, Haijun Liu","doi":"10.1007/s11120-025-01153-6","DOIUrl":"https://doi.org/10.1007/s11120-025-01153-6","url":null,"abstract":"<p><p>PsbR is a nonpigmented 10 kDa protein in Photosystem II (PSII) in algae and plants. A recent structural study clarified its enigmatic structural location in a Photosystem II megacomplex that has baffled the community for more than four decades. Our current study interrogates whether absence of PsbR affects the overall dynamics of excitation energy migration within light harvesting complexes (LHC) and PSII super assemblies using highly-active PSII membrane particles, so-called BBY particles, isolated from a PsbR deletion mutant (ΔPsbR) of Arabidopsis thaliana. A femto-second (fs)-time-resolved transient absorption experimentation recorded at 77 K with selective excitation of Chl b which is exclusively present in LHCs enabled us to resolve the temporal differences in LHC→LHC and LHC→PSII excitation energy transfer steps. By applying specific target spectro-kinetic models to the transient absorption datasets, we demonstrated that the time constants of Chl a_LHC → Chl a_LHC excitation transfer significantly elongates in the ΔPsbR LHC-PSII particles, suggestive of the decreased aggregation level of photosynthetic proteins in the mutant. These findings highlight excitation energy transfer integrity in LHC-PSII assembly is not only determined by the pigmented light-harvesting complexes, but also synergistically by the nonpigmented PSII components. The disturbed integrity in dynamics of excitation energy transfer pathway within LHC-PSII supercomplex is discussed in the context of the altered LHC-PSII megacomplexes type I and II architectures which result from the absence of the PsbR protein in higher plant PSII.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"31"},"PeriodicalIF":2.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic capacity and pigment distribution of a siphonous green alga, Dichotomosiphon tuberosus.","authors":"Soichiro Seki, Koichi Kobayashi, Ritsuko Fujii","doi":"10.1007/s11120-025-01148-3","DOIUrl":"10.1007/s11120-025-01148-3","url":null,"abstract":"<p><p>Dichotomosiphon tuberosus is one of the Bryopsidales, a siphonous, unicellular multinucleate ulvophyte. Bryopsidales typically occur in the ocean and contain unique carbonyl carotenoids siphonaxanthin (Sx) and its ester siphonein (Sn) in their major light-harvesting pigment-protein complexes, allowing them to utilize the green light available in the deep ocean for photosynthesis. However, unlike other Bryopsidales, D. tuberosus occurs in fresh water and is reported to contain Sn but not Sx. D. tuberosus inhabits deep lakes around the world, but in Okinawa, Japan, it inhabits very shallow waterways. Here, we measured the photosynthetic capacity of D. tuberosus collected from Okinawa waterway and compared it with another intertidal Bryopsidale Codium fragile. D. tuberosus had higher photosynthetic electron transport capacity and stronger non-photochemical quenching than C. fragile, consistent with the brighter growth environments for D. tuberosus than C. fragile. We also measured the absorption spectra and the pigment compositions within the photosynthetic pigment-protein complexes from D. tuberosus. Green light absorption of each complex in D. tuberosus was weaker than that in C. fragile. In contrast, Chl b absorption in LHCII was stronger in D. tuberosus than in C. fragile, whereas the opposite was true in photosystems. This implies that a large proportion of the irradiated energy is absorbed by LHCII complex and quenched more efficiently. Our results indicate that the photosynthetic capacity of D. tuberosus is further optimized for higher light environments compared with C. fragile.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"30"},"PeriodicalIF":2.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating photosynthetic and chlorophyll fluorescence responses to light in peanut acclimated to elevated CO₂ and temperature.","authors":"Rajanna G Adireddy, Saseendran S Anapalli, Christopher D Delhom, Naveen Puppala, Krishna N Reddy","doi":"10.1007/s11120-025-01151-8","DOIUrl":"https://doi.org/10.1007/s11120-025-01151-8","url":null,"abstract":"<p><p>In plants, the photo-inhibitory effects of incident lights on the light-harvesting complexes are balanced by photoprotective mechanisms to maintain photosynthesis. With increasing air CO₂ concentrations and temperatures, the balance can tilt either way, with unpredictable consequences for biomass assimilated through photosynthesis. As such, it is critical to assess the photosynthetic responses of crop plants growing in future climates to light for developing strategies for sustaining food production. This study evaluated changes in photosynthetic and chlorophyll fluorescence responses to light intensities in peanuts (Arachis hypogaea L) acclimated to projected future climates by Global Circulation Models (GCM). The plants were grown in plant growth chambers under three climate conditions (CC): (1) ambient air [CO₂] and ambient temperature [Ta] (CC1), (2) [CO₂] at 570 ppm and Ta + 3⁰ C (CC2 climate possible in 2050), and (3) [CO₂] at 780 ppm and Ta + 5⁰C (CC3, climate possible in 2080). Plants growing under all three climates enhanced photosynthetic rates (A) with light intensities from 0 to 1500 µ mol m^- 2 s^- 1 but decreased afterward. Compared to CC1, plants growing under CC2 and CC3 reduced electron transport rates (ETR), A, and transpiration (Tr) between 48 and 190%, 52 and 65%, and 22 and 24%, respectively. Concurrently, the quantum efficiency of photosystem II (ФPS2) was reduced by 88-200% and photochemical quenching (qP) by 55-170%. Non-photochemical quenching increased with increasing light levels from 200 to 1500 µmol m⁻² s⁻¹ and decreased afterward. Results indicated the possibility of reduced photosynthetic efficiencies under CC2 and CC3, which would significantly reduce biomass production in future climates. Gaining insight into these impacts can help understand plant's ability to adapt and assist in developing adaptive strategies for sustainable peanut farming.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"29"},"PeriodicalIF":2.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing substrate water access through the O1 channel of Photosystem II by single site mutations and membrane inlet mass spectrometry.","authors":"A Orkun Aydin, Casper de Lichtenberg, Feiyan Liang, Jack Forsman, André T Graça, Petko Chernev, Shaochun Zhu, André Mateus, Ann Magnuson, Mun Hon Cheah, Wolfgang P Schröder, Felix Ho, Peter Lindblad, Richard J Debus, Fikret Mamedov, Johannes Messinger","doi":"10.1007/s11120-025-01147-4","DOIUrl":"https://doi.org/10.1007/s11120-025-01147-4","url":null,"abstract":"<p><p>Light-driven water oxidation by photosystem II sustains life on Earth by providing the electrons and protons for the reduction of CO₂ to carbohydrates and the molecular oxygen we breathe. The inorganic core of the oxygen evolving complex is made of the earth-abundant elements manganese, calcium and oxygen (Mn₄CaO₅ cluster), and is situated in a binding pocket that is connected to the aqueous surrounding via water-filled channels that allow water intake and proton egress. Recent serial crystallography and infrared spectroscopy studies performed with PSII isolated from Thermosynechococcus vestitus (T. vestitus) support that one of these channels, the O1 channel, facilitates water access to the Mn₄CaO₅ cluster during its S₂→S₃ and S₃→S₄→S₀ state transitions, while a subsequent CryoEM study concluded that this channel is blocked in the cyanobacterium Synechocystis sp. PCC 6803, questioning the role of the O1 channel in water delivery. Employing site-directed mutagenesis we modified the two O1 channel bottleneck residues D1-E329 and CP43-V410 (T. vestitus numbering) and probed water access and substrate exchange via time resolved membrane inlet mass spectrometry. Our data demonstrates that water reaches the Mn₄CaO₅ cluster via the O1 channel in both wildtype and mutant PSII. In addition, the detailed analysis provides functional insight into the intricate protein-water-cofactor network near the Mn₄CaO₅ cluster that includes the pentameric, near planar 'water wheel' of the O1 channel.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"28"},"PeriodicalIF":2.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UVR regulation of photoprotection in Phaeodactylum tricornutum (Bacillariophyceae): roles of light energy doses.","authors":"Herculano Cella, José Bonomi-Barufi, Camila Lisarb Velasquez Bastolla, Camila Nader, Carlos Yure Oliveira, Rafael Garcia Lopes, Jacó Joaquim Mattos, Paulo Antunes Horta Junior, Henrique Cesar Venâncio, Eva Regina de Oliveira Rodrigues, Marcelo Maraschin, Leonardo Rubi Rörig, Afonso Celso Dias Bainy, Maria Risoleta Freire Marques, Roberto Bianchini Derner","doi":"10.1007/s11120-025-01149-2","DOIUrl":"https://doi.org/10.1007/s11120-025-01149-2","url":null,"abstract":"<p><p>The ability of diatoms to adapt to variable light conditions such as photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) is crucial for their survival and ecological role. The study aimed to investigate how different doses of UVR regulate the photoprotection mechanism in Phaeodactylum tricornutum. Five treatments were established: Control (PAR only), PAR + UVR D_6h (17.5 W m^- 2, 6 h, 378 KJ m^- 2), PAR + UVR 2D_6h (35 W m^- 2, 6 h, 756 KJ m^- 2), PAR + UVR 2D_12h (17.5 W m^- 2, 12 h, 756 KJ m^- 2), and PAR + UVR 4D_12h (35 W m^- 2, 12 h, 1.512 KJ m^- 2). The growth of P. tricornutum was significantly affected by UVR doses, with a growth pattern of 42-55% below the control (PAR only). Increasing the UVR dose also had negative effects on photosynthesis parameters and the levels of chlorophyll-a and fucoxanthin. The de-epoxidation state showed a high rate in treatments subjected to higher UVR doses, promoting an attempt to activate cellular protection mechanisms. In the transcriptional genes related to the xanthophyll cycle, a reduction in transcript levels of ZEP1 and ZEP2 genes was observed in PAR + UVR treatments, including a reduction up to 75% at 72 h of exposure. Also, an increase in transcript levels of VDE and VDL1 genes was observed for treatments with the same UVR dose, reaching about 9-fold increase for the 2D_12h dose and 10-fold increase for the 2D_6h dose, both at 72 h, suggesting a modification in the cell's ability to respond to UVR light stress.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"27"},"PeriodicalIF":2.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.","authors":"João Serôdio","doi":"10.1007/s11120-025-01150-9","DOIUrl":"https://doi.org/10.1007/s11120-025-01150-9","url":null,"abstract":"<p><p>In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"26"},"PeriodicalIF":2.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Expression of Concern: Excess copper induces anoxygenic photosynthesis in Anabaena doliolum: A homology based proteomic assessment of its survival strategy.","authors":"","doi":"10.1007/s11120-025-01146-5","DOIUrl":"10.1007/s11120-025-01146-5","url":null,"abstract":"","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 2","pages":"25"},"PeriodicalIF":2.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoperiod-mediated rapid generation advancement in soybean (Glycine max (L.) Merr.).","authors":"Ambika Rajendran, Ayyagari Ramlal, Dhandapani Raju, Manisha Saini, Pinkal Bishnoi, Sreeramanan Subramaniam","doi":"10.1007/s11120-025-01144-7","DOIUrl":"10.1007/s11120-025-01144-7","url":null,"abstract":"<p><p>Soybean is a short-day crop and the long-duration variety takes 120 days for maturity. A protocol for rapid generation advancement in soybean breeding is worthwhile keeping in view its utility. The study emphasizes standardisation of physical conditions, especially using warm white and cool white light-emitting diodes to hasten flowering and pod setting in soybean (DS9712 genotype). Complete open flowers were obtained with a 16 L/8D (dark/light) photoperiod in 30 days. The results also highlighted the application of interventions of physical conditions and inputs, especially during the reproductive phase to shorten the seed-to-seed generation time by around 15 days in a low-cost method for soybean breeding. Breeding will be revolutionised in case economic speed breeding is combined with modern breeding technologies, thereby resulting in more generations per year.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 2","pages":"24"},"PeriodicalIF":2.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly of allophycocyanin from individual subunits: involvement of apo-form in the formation of trimers.","authors":"Daria V Bodunova, Daniil A Gvozdev, Olesya V Bukach, Svetlana V Sidorenko, Kristina V Perfilova, Li Yu, Song Qin, Nikolai N Sluchanko, Baosheng Ge, Eugene G Maksimov","doi":"10.1007/s11120-025-01145-6","DOIUrl":"10.1007/s11120-025-01145-6","url":null,"abstract":"<p><p>In cyanobacteria and red algae, allophycocyanin (APC), as well as other phycobiliproteins, is involved in the energy transfer of photosystems. Since APC is a potent fluorescent protein for imaging and biomedical applications, it is necessary to obtain purified protein in large quantities, which is currently possible by biosynthesis in bacterial systems. Here we emphasize the challenges of obtaining the trimeric form of the protein from α-APC and β-APC subunits of allophycocyanin in vitro. This approach allowed us to study the individual subunits and to perform assembly of allophycocyanin trimers in vitro. Using different spectroscopic techniques, we detected the heterogeneity of the synthesized β-APC and showed the possibility that not only holo-forms may be involved in trimer formation. Data allowed us to provide additional arguments in favor of excitonic coupling of chromophores in APC trimers.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 2","pages":"23"},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}